Ecological Genetics and Genomics最新文献

{"title":"Reviews on Asian citrus species: Exploring traditional uses, biochemistry, conservation, and disease resistance","authors":"Piumi Chathurika Palangasinghe ,&nbsp;Wasantha Kumara Liyanage ,&nbsp;Manupa Pabasara Wickramasinghe ,&nbsp;Hasini Ruweeeka Palangasinghe ,&nbsp;Huie-Chuan Shih ,&nbsp;Meng-Shin Shiao ,&nbsp;Yu-Chung Chiang","doi":"10.1016/j.egg.2024.100269","DOIUrl":"https://doi.org/10.1016/j.egg.2024.100269","url":null,"abstract":"<div><p>The species of genus <em>Citrus</em> mostly originated in Southeast Asia, a region with a remarkable diversity of <em>Citrus</em> species. They have been cultivated and harnessed worldwide and are commonly known as mandarins, oranges, lemons, and pomelos. The major products of the plants include the juice from fruits, leaves, and the rind as flavour enhancement of food in many regional culinary cultures. Different parts of the plants are commonly used in traditional medicines with a long history ascribed to the potential medicinal benefits, including antimicrobial, antihypertensive, anti-inflammatory, and antioxidant properties. In this article, we aim to provide a comprehensive review of studies on <em>Citrus</em> species in the following aspects. First, we reviewed studies on various <em>Citrus</em> species in different geographic regions of Asia including their uses in medicinal, culinary, and other cultural purposes. The phylogeny of the <em>Citrus</em> species was proposed to be complicated, which might be attributed to the extensive interbreeding between ancestral taxa and/or being cultivated for different economic strains. Secondly, we summarized and discussed the studies of <em>Citrus depressa</em> in Japan and Taiwan, emphasizing the maintenance of genetic diversity and the potential health benefits of bioactive compounds. Finally, we reviewed the studies discovering the species or cultivars that may provide a source of disease-resistant genes to help <em>Citrus</em> plants from Huanglongbing (HLB), a common disease of <em>Citrus</em> species. The use of the latest genomic biotechnology to introduce disease-resistant genes to cultivars will significantly reduce the damage to the soil and environment from using chemical reagents for disease prevention.</p></div>","PeriodicalId":37938,"journal":{"name":"Ecological Genetics and Genomics","volume":"32 ","pages":"Article 100269"},"PeriodicalIF":0.0,"publicationDate":"2024-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141241767","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome-wide association studies reveals new candidate genes associated with resistance to Striga gesneroides in Cowpea [Vigna unguiculata (L.) Walp.] accessions from sub-Saharan Africa","authors":"Abdoulaye Ali Koura ,&nbsp;Alexander Wireko Kena ,&nbsp;Benjamin Annor ,&nbsp;Idris I. Adejumobi ,&nbsp;Abdoul- Raouf Sayadi Maazou ,&nbsp;Fredercik Justice Awuku ,&nbsp;Patrick Attamah ,&nbsp;Ousmane Boukar ,&nbsp;Akromah Richard","doi":"10.1016/j.egg.2024.100267","DOIUrl":"https://doi.org/10.1016/j.egg.2024.100267","url":null,"abstract":"<div><p>Parasitic plant <em>Striga gesneroides</em> reduces cowpea productivity in sub-Saharan Africa, leading to substantial yield losses. This weed is the major reason for land abandonment by many farmers. While various methods have been proposed for managing <em>Striga</em> invasion and parasitism, host-plant resistance remains the most effective and affordable option for small-scale farmers in the sub-region. This study aims to expedite the improvement of <em>Striga</em> resistance breeding through marker-assisted selection by identifying genomic regions and candidate genes associated with <em>Striga</em> resistance indicator traits using association mapping. A panel of 188 cowpea accessions assembled from five gene banks in West Africa were phenotyped for two years under <em>Striga</em>-infested and non-infested research conditions. These accessions were equally genotyped using the medium-density genotyping of the Diversity Arrays Technology sequencing (DArTseq) platform to obtain marker information. High broad-sense heritability estimates were observed for <em>Striga</em> resistance status (SRS), number of <em>Striga</em> per plot (NSPlot), and Pod weight (PW) while low heritability estimates were observed for fodder weight (FW) and Plant Aspect (PASP). Twenty-four SNP markers were identified across SRS, NSPlot, FW, PASP, and PW under <em>Striga</em>-infested conditions. Under non-infested conditions, 17 SNPs were identified across FW, PASP, and PW. Gene annotation of the significant SNPs revealed candidate genes such as ubiquitin ligase activity, cell wall reinforcement protein, and pathogenesis-related protein. These genes function in plant growth regulation, development, and defense mechanisms. This study offers valuable insights for marker selection, validation, and deployment in cowpea improvement for <em>Striga</em> resistance in SSA.</p></div>","PeriodicalId":37938,"journal":{"name":"Ecological Genetics and Genomics","volume":"32 ","pages":"Article 100267"},"PeriodicalIF":0.0,"publicationDate":"2024-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141241766","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Two novel heat-tolerant, culturable rhizosphere-inhabiting, plant growth-promoting ascomycete fungi associated with native plants in desert habitats of southwestern USA","authors":"Mojtaba Mohammadi ,&nbsp;Michael E. Stanghellini","doi":"10.1016/j.egg.2024.100265","DOIUrl":"https://doi.org/10.1016/j.egg.2024.100265","url":null,"abstract":"<div><p>During an investigations on <em>Monosporascus cannonballus</em>, the causal agent of root rot and vine decline of melons, in 1999, a previously unknown rhizosphere fungus was encountered in the desert soils of southern California. The fungus, herein named <em>Desertella arida</em> Stanghellini &amp; Mohammadi sp. nov., was associated with roots of diverse plants including brassica (Sahara mustard (<em>Brassica tournefortii</em>), shepherd's purse (<em>Capsella bursa</em>-<em>pastoris</em>)) and plantain (<em>Plantago ovata</em> Forsk). The fungus forms extensive multinucleate, septate hyphae (6–17 μm in diameter) bearing hundreds of large spherical to pear-shaped unicellular asexual spores (50–120 μm in diameter), either singly or in large clusters. The spores are beige to cream-colored, thin-walled, and contain a dense, granular cytoplasm. Further investigation on the distribution of <em>D. arida</em> in soils of desert habitats in the southwestern USA led to the discovery of a second fungus belonging to a different species herein named <em>Desertella americanense</em> Mohammadi &amp; Stanghellini sp. nov.<em>,</em> in California, Arizona, Nevada, and Utah. The two species are morphologically similar. Spores from both fungi in native soil survived heat treatment up to 100 °C. Both species are culturable and colonized roots of monocots and dicots forming abundant hyphae and spores as early as 10 days post-inoculation. <em>Desertella arida</em> significantly enhanced shoot growth in broccoli, lettuce, <em>Plantago ovata</em> and flax. In conclusion, we discovered two desert-dwelling ascomycete fungi that were associated with the roots of native plant species in southern California. They were culturable on a synthetic medium, heat-tolerant and promoted growth on several crop species. These fungi appeared to exist in other neighboring states such as Arizona and Nevada.</p></div>","PeriodicalId":37938,"journal":{"name":"Ecological Genetics and Genomics","volume":"32 ","pages":"Article 100265"},"PeriodicalIF":0.0,"publicationDate":"2024-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2405985424000491/pdfft?md5=ddca020cf8030d48fe75e77e41a9d3bf&pid=1-s2.0-S2405985424000491-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141095083","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessing salinity tolerance and genetic variation in mung bean (Vigna radiata) through CAAT box and SCoT marker analysis","authors":"Elangovan Dilipan ,&nbsp;A. Jawahar Nisha","doi":"10.1016/j.egg.2024.100266","DOIUrl":"10.1016/j.egg.2024.100266","url":null,"abstract":"<div><p><em>Vigna</em> is a genus of approximately 100 wild species found in tropical and sub-tropical region. The three varieties of green gram (<em>Vigna radiata</em> L.) (VBN3, VBN6 &amp; CO8) was widely cultivated in Tamil Nadu. Soil salinity is a significant issue in food production because excessive salt concentrations in soil inhibit plant development, delaying maturity and reducing crop yield or causing wilting. In the present study, we investigate the effective screening of saline tolerance in green gram, carried out the mechanism of physiological, biochemical and genetic variation using molecular markers of salinity stress of green gram under different concentration of NaCl (control, 50, 100, 150 and 200 mM). The result shows that, the salinity stress significantly reduced the physiological, biochemical and plant growth characteristics. Besides, the gene-targeted like Start Codon Targeted (SCoT) and CAAT box derived polymorphism (CBDP) markers were used to examine the genetic variability under saline stress of green gram. Herein, the SCoT and CBDP primers produced 54 and 90 polymorphic bands with an average of 5.7 and 7.8 bands per primer, respectively. The Analysis of Molecular Variance (AMOVA) and Principal Coordinate Analysis (PCoA) revealed genetic diversity within species was greater than between them, indicating that all of the saline-treated samples from the three varieties displayed genetic variation. The present study concludes that the increased concentration of NaCl reduced the plant growth and significantly affects the physiological, biochemical and genetic variation.</p></div>","PeriodicalId":37938,"journal":{"name":"Ecological Genetics and Genomics","volume":"32 ","pages":"Article 100266"},"PeriodicalIF":0.0,"publicationDate":"2024-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141139869","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unraveling genotype-by-environment interaction in maize using cutting edge statistical tools: Innovative empirical selection for increased yield stability","authors":"Chitra Bahadur Kunwar ,&nbsp;Bikas Basnet ,&nbsp;Samjhana Sunuwar ,&nbsp;Deep Narayan Mahato ,&nbsp;Ramdas Chaudhari ,&nbsp;Jharna Upadhya ,&nbsp;Pragya Pokhrel","doi":"10.1016/j.egg.2024.100249","DOIUrl":"https://doi.org/10.1016/j.egg.2024.100249","url":null,"abstract":"<div><p>By prioritizing high yields in ideal conditions, traditional breeding might miss out that genotypes with superior yield and stability. Thus,this study seeks to identify stable genotypes that offer more predictable and reliable yields; under both stress and resourceful environments, leading to not only greater food security but financial stability as well. Incorporating both fixed (AMMI&amp;GGI) and random effects models (BLUP &amp; MTSI), not only leveraged theoretical but also empirical comparisons-weakness of each model is covered by all 4 models-to select elites hybrids Maize. Furthermore, a multi-environment trial assessed 41 hybrids across 4 research stations in 2 winter seasons using RCB design. Likewise, compiled cutting-edge statistical methods for GEI analyses from the “metan” package (R-Software), selected hybrids with consistent performance with meticulous analysis. AMMI(Additive Main effects and Multiplicative Interaction)analysis revealed that of significant (p ≤ 0.05) effects of genotype, environment, &amp; genotype-environment interaction (GEI), explaining yield variance with interaction effects were captured by 3 interaction principal components (IPCs). Moreover,GEI graphics of AMMI biplots elucidates four hybrids(MRM-4062, Super-22, PAC-744, and Gen-4118) with high yield stability and above-average performance. Additionally, eight hybrids (4118, 4558, 5454, NMH1258, PAC 746, PAC 745 Gold, and SUPER 9090) exhibited superior yield, regardless of their stability. Nepalgunj exhibited the lowest GEI value. Conversely, Rampur displayed an above-average GEI, indicating a strong ability to modulate varieties performance. Besides this, GGE biplot revealed two potential mega-environments based on yield similarities. Nine high-yielding hybrids (SUPPER 6768, SUPPER 9396, PAC750, MBS5622, PAC745 Gold, MRM4065, NMH8392, Gen-4118, and Gen-4558) were identified as vertex hybrids, signifying their superior performance across wide environments. Even supposing, mixed effects model, both the best linear unbiased prediction (BLUP) weighted with average absolute score standardized by yields (WAASBY) models, Gen-4118 ranked highest in terms of average yield, followed by NMH-8392, TMH2858, and others, and Multi-Trait Stability Index (MTSI) identified SUPPER-9090 as the most stable and high-yielding genotype, followed by NMH4040, Super-22, Gen-4118, MBS-1144, and NMH1255. In a nutshell, Gen-4118, Super-9090, MRM-4062, NMH1258, NMH-8392, TMH2858, PAC 746, PAC 745 Gold emerge as the highest-yielding and most consistently high-performinghybrids, offering promise for commercial farming across diverse environments. Clearly,this study has potential to mitigate risks for farmers (economic burden, crop failure) and bolster food security/climate resilience. What's more, it paves the way for future research on cold tolerance genes for even greater crop improvement.</p></div>","PeriodicalId":37938,"journal":{"name":"Ecological Genetics and Genomics","volume":"32 ","pages":"Article 100249"},"PeriodicalIF":0.0,"publicationDate":"2024-05-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141084627","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluating the genetic variability in selected accessions of medicinal legume Mucuna pruriens (L) DC through ISSR markers","authors":"N. Seema Devi ,&nbsp;Ravi Rajwanshi ,&nbsp;K.P. Mohapatra","doi":"10.1016/j.egg.2024.100262","DOIUrl":"10.1016/j.egg.2024.100262","url":null,"abstract":"<div><p><em>Mucuna pruriens</em>, a widely available but underutilized legume in India, stands out for its nutritional and medicinal potential. Understanding the genetic diversity of <em>M. pruriens</em> is vital for its conservation and utilization in breeding programs aimed at enhancing its nutritional and agronomic traits. In this regard, molecular markers like ISSRs offer a reliable and informative tool for assessing genetic diversity. In the present study, 23 <em>M. pruriens</em> accessions from northeastern India were evaluated using ISSR markers, revealing high levels of polymorphism and significant genetic diversity. A total of 512 amplicons were generated across 33 ISSR primers, with an average polymorphic percentage of 98.05 %. PIC values ranged from 0.22 to 0.50, indicating substantial genetic variation within the accessions. Supplementary indicators such as EMR and MI were employed to evaluate the marker system's efficacy. EMR ranged from 6 to 22, averaging 14.95, while MI ranged from 2.64 to 9.02, averaging 6.04, with a positive correlation observed between EMR and MI. Cluster analysis based on Jaccard's similarity coefficient classified the accessions into two major groups, with the northeastern Indian accessions displaying higher diversity compared to those from other regions of India. Interestingly, geographical proximity did not consistently correlate with genetic similarity, suggesting complex patterns of gene flow and historical germplasm exchange. Overall, this study provides valuable insights into the genetic diversity of <em>M. pruriens</em> and highlights the importance of conserving and utilizing this underutilized legume for addressing global food security challenges.</p></div>","PeriodicalId":37938,"journal":{"name":"Ecological Genetics and Genomics","volume":"32 ","pages":"Article 100262"},"PeriodicalIF":0.0,"publicationDate":"2024-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141051860","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genotype-by-environment interaction analysis for grain yield of barley genotypes in the warm climate of Iran","authors":"Alireza Pour-Aboughadareh ,&nbsp;Akbar Marzooghian ,&nbsp;Ahmad Gholipour ,&nbsp;Hassan Zali ,&nbsp;Shirali Koohkan ,&nbsp;Ali Omrani ,&nbsp;Masoome Kheirgoo ,&nbsp;Alireza Askari-Kelestani ,&nbsp;Ali Barati","doi":"10.1016/j.egg.2024.100264","DOIUrl":"10.1016/j.egg.2024.100264","url":null,"abstract":"<div><p>In the present study, multi-environmental trials were performed on 19 newly developed barley genotypes at five test regions across the warm climate in Iran for two consecutive years. The results of additive main effects and multiplicative interaction (AMMI), GGE biplot (GGE) model, and several BLUP-based stability parameters and integrated models (WAASB and WAASBY) were used to reveal GEI patterns and identify superior genotypes with stable grain yield across test environments. Statistical analyses revealed that grain yield was influenced by significant environments (E), genotypes (G), and GEI effects. Among AMMI-, BLUP-, and grain mean-based stability parameters, the harmonic means of genotypic values (HMGV), relative performance of genotypic value (RPGV), and harmonic mean of RPGV parameters indicated a <em>dynamic</em> concept of stability. Moreover, results of GGE biplot analysis identified three mega-environments with different winner genotypes in test environments. Regardless of the different years, the Ahvaz, Darab, and Moghan regions were identified as Type-II environments due to their highest discrimination power and representative ability. The grouping pattern considerably followed the ranking of genotypes in GGE biplot analysis and WAASB model. In conclusion, our results revealed that genotypes G9 and G10 had grain yield higher than the reference genotype and relative stability in specific environments with favorable environmental conditions. Genotype G5 had general adaptability and stability across all test environments. This genotype can be recommended for cultivation in a wide range of environments in the warm climate of Iran.</p></div>","PeriodicalId":37938,"journal":{"name":"Ecological Genetics and Genomics","volume":"32 ","pages":"Article 100264"},"PeriodicalIF":0.0,"publicationDate":"2024-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141047194","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Distribution and diversity studies of endophytic and rhizospheric fungi associated with the mangrove, Acanthus ilicifolius, using amplicon sequencing-based metagenomic approach","authors":"Sagar P. Shah ,&nbsp;Jacinta Teresa George ,&nbsp;Jayaprada Rao Chunduri","doi":"10.1016/j.egg.2024.100261","DOIUrl":"https://doi.org/10.1016/j.egg.2024.100261","url":null,"abstract":"<div><p>Fungal endophytes exhibit a broad diversity and are found universally associated with all plant species, playing a vital role in enhancing the host plant's resilience against both biotic and abiotic stressors. In this investigation conducted in Mumbai, India, the Illumina MiSeq sequencing platform was employed to study the endophytic fungal diversity of the common mangrove, <em>Acanthus ilicifolius</em> and its rhizosphere utilizing the amplification of the fungal ribosomal ITS2 region. The results revealed the creation of 307 Operational Taxonomic Units (OTUs) from a total of 663,600 sequencing reads, clustered at a 97 percent similarity level. Evaluation using Simpson and Shannon indices indicated that the highest community diversity was observed in stem tissue compared to root and leaf samples. The total OTUs were categorized into five phyla, 41 orders, 89 families, and 133 genera, with <em>Ascomycota</em> being the predominant phylum at 76.9% relative abundance, followed by <em>Basidiomycota</em> at 22.87%. The most abundant genera in the soil were <em>Malassezia</em> (35%) and <em>Aspergillus</em> (29.5%), while <em>Vishniacozyma</em> (61%) dominated in leaf samples. Unlike stem and root, no single genus dominated these tissues. The findings demonstrated significant distinctions between the fungal endophytic communities in plant tissues and the fungal microbiome in the soil. Notably, the endophyte fungi community in roots exhibited a closer resemblance to stem samples than to leaf samples. FunGuild analysis also revealed characteristic communities in different samples indicating specific functions of these genera. Overall, this study provides novel insights into the intricate composition of endophytic fungi within the tissues of <em>A. ilicifolius</em> and its rhizosphere.</p></div>","PeriodicalId":37938,"journal":{"name":"Ecological Genetics and Genomics","volume":"31 ","pages":"Article 100261"},"PeriodicalIF":0.0,"publicationDate":"2024-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140914219","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Precise and consistent genotype identification for essential oil yield and ursolic acid in a multi-year assessment of basil","authors":"R.K. Lal,&nbsp;C.S. Chanotiya,&nbsp;Pankhuri Gupta,&nbsp;Ashish Kumar","doi":"10.1016/j.egg.2024.100260","DOIUrl":"https://doi.org/10.1016/j.egg.2024.100260","url":null,"abstract":"<div><p>In the current study, sixteen basil genotypes in India were analyzed over three years using the AMMI stability model. The study years explained the bulk of the variation in all nine variables. The vast amount of data required for this inquiry hinders plant breeding processes. For basil's two most critical features, ursolic acid and essential oil yield, we found that genotypes 6, 11, and 12 were the best and most consistent options for practically every analyzed property. Furthermore, there were substantial + ve and -ve correlations among the major characteristics that drove stable line selection. Trait associations and stability-related factors both influence stable genotype selection in basil. Based on the results, genotypes 6, 11, and 12 may be recommended for large-area cultivation.</p></div>","PeriodicalId":37938,"journal":{"name":"Ecological Genetics and Genomics","volume":"31 ","pages":"Article 100260"},"PeriodicalIF":0.0,"publicationDate":"2024-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140900933","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genealogical structure and genetic variability: A step towards conservation of white Morada Nova sheep in semi-arid region","authors":"Daniel Caetano Sales ,&nbsp;José Ernandes Rufino de Sousa ,&nbsp;Marcos Aurelio Victor de Assunção ,&nbsp;Natanael Silva Félix ,&nbsp;Bianca Ferreira do Nascimento ,&nbsp;Wanderson Lucas Alves dos Santos ,&nbsp;Antonia Gessica Beatriz de Araujo Noronha ,&nbsp;Robson Mateus Freitas Silveira ,&nbsp;Concepta McManus ,&nbsp;Débora Andréa Evangelista Façanha","doi":"10.1016/j.egg.2024.100256","DOIUrl":"https://doi.org/10.1016/j.egg.2024.100256","url":null,"abstract":"<div><p>Morada Nova breed has a low effective herd, and its white variety is in risk of extinction in Brazilian semi-arid region. This study aimed to evaluate white Morada Nova sheep’s genetic variability and population structure in four flocks. These are the only flocks in the world. The assessed parameters of pedigree integrity and a genealogy record of 219 animals. 44.74 % did not have pedigree information on their mother or father, and 50.47 % did not have available information on their grandparents. 52.97 % had a pedigree in their first ancestry (obtained through the average of known parents), 23.74 % in the second, 13.58 % in the third, and 5.33 % in the fourth. The effective size was 10.7, considering the complete equivalent generation. The effective number of founding animals (fe) and ancestors (fa) was 10, indicating the effective participation of all founding animals in the population over the generations, reducing losses of genetic variability. Out of all the ancestors, only 3 explained 50 % of the population’s genetic variability, reflecting the low values of fa and fe. The population’s average inbreeding and kinship coefficients were 2.61 and 4.54. The F statistics of Wright, Fis, Fst, and Fit, were −0.012, 0.016, and 0.003. The genetic conservation index (GCI) had an average of 2.01 ± 1.29, with minimum and maximum values of 1.0 and 6.38, respectively. Approximately, 71 % of the population had GCI below 2.0, while only 5.9 % of sheep had an GCI bigger than 5.0. This indicates the absence of sub-structures inf the population. There is no population subdivision, considering the genealogical structure of the evaluated flocks. The inbreeding and kinship coefficient values are controlled and serve as a starting point for establishing a future management plan for the genetic variability of the flock. It is recommended to use strategies to increase the effective population size, to meet the minimum number recommended by FAO and to promote the conservation and genetic improvement of the breed.</p></div>","PeriodicalId":37938,"journal":{"name":"Ecological Genetics and Genomics","volume":"31 ","pages":"Article 100256"},"PeriodicalIF":0.0,"publicationDate":"2024-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140894267","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}