Ecological Genetics and Genomics最新文献

{"title":"Analysis of genetic variability among regional mango varieties grown in Rajshahi district using RAPD markers","authors":"Md Abuhena ,&nbsp;Jubair Al-Rashid ,&nbsp;Abul Kalam Azad","doi":"10.1016/j.egg.2024.100258","DOIUrl":"https://doi.org/10.1016/j.egg.2024.100258","url":null,"abstract":"<div><p>The evaluation of genetic relatedness and diversity is a crucial step in describing and utilizing germplasm for the development of new varieties. In this study, the genetic diversity of four indigenous mango varieties was investigated. Three RAPD primers were used to perform the DNA fingerprint after the DNA was extracted from the leaf sample using the modified CTAB procedure. Cluster analysis was carried out using the unweighted pair-group method of arithmetic means (UPGMA). According to the results, the highest amount of DNA was recovered from the Fazli cultivar (1142.7 ± 0.6 ng/μl). On amplification, the RAPD analysis revealed 25 scoreable bands, of which twenty (20) were monomorphic and five (5) were polymorphic. The primer OPA-03 had the highest polymorphism of 23 %, whereas OPD-04 had no band. The Amropali versus Fazli variety pair had the highest linkage distance (4.69), whilst the Ashawina versus Lengra variety pair had the lowest linkage distance (2.00). At a linkage distance of 4.25, the dendrogram revealed the segregation of the four mango cultivars into two major clusters. Amropali was placed in cluster 1 (C1) and Ashawina, Fazli, and Lengra were placed in cluster 2 (C2) demonstrates that the Amropali variety is distinct from all others. According to the findings, this research could potentially provide insight about the regional mango diversity and be helpful for future mango breeding programs.</p></div>","PeriodicalId":37938,"journal":{"name":"Ecological Genetics and Genomics","volume":"32 ","pages":"Article 100258"},"PeriodicalIF":0.0,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140948064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Estimation of genetic diversity utilizing gene-targeted SCoT markers and morpho-chemotypic analyses in Senna alexandrina Mill. (Senna)","authors":"Ashish Kumar ,&nbsp;Anil Kumar Gupta ,&nbsp;Saba Siddiqui ,&nbsp;Soni Gupta ,&nbsp;R.K. Lal ,&nbsp;Abhilasha Srivastava ,&nbsp;Mohammed Haris Siddiqui","doi":"10.1016/j.egg.2024.100252","DOIUrl":"https://doi.org/10.1016/j.egg.2024.100252","url":null,"abstract":"<div><p>Senna, an industrial crop that generates foreign exchange, is planted predominantly in southern India. Senna leaves and pods grown in India are valued in international trade and the global market because they contain a high concentration of the chemical component sennosides. The current investigation was conducted in the CSIR-CIMAP experimental farm in Hyderabad, India. The experiment was designed in an RCBD and replicated three times using twenty-five senna accessions in the years 2020–21 and 2021–22. The combined ANOVA found that all fourteen of the traits studied were highly significant. The D2 analysis and λ1-λ2 values classified the 25 accessions into five clusters with 91.05 % diversity. The molecular diversity was also examined using SCoT markers. As SCoT markers target expressed portions of the genome, the variation among accessions reflects some of the morphological changes among them. For pharmaceutical applications, various lines Gen-1 and 2 (high in Sennosides A and B content) and Gen-4 can be hybridized with Gen-5 and 25 to produce generations high in pharmaceutically relevant Sennosides A and B. Considering genotypes Gen 1 and 2 rank first and second in terms of sennoside A and B contents in leaves (%) and pods, these two genotypes can be employed for large-scale cultivation and to transfer favorable traits in future breeding programs.</p></div>","PeriodicalId":37938,"journal":{"name":"Ecological Genetics and Genomics","volume":"31 ","pages":"Article 100252"},"PeriodicalIF":0.0,"publicationDate":"2024-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140813457","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Developments in extremophilic bacterial genomics: A post next generation sequencing era","authors":"Digvijay Verma ,&nbsp;Swati Joshi ,&nbsp;Priyanka Ghimire ,&nbsp;Archana Mishra ,&nbsp;Vinay Kumar","doi":"10.1016/j.egg.2024.100255","DOIUrl":"https://doi.org/10.1016/j.egg.2024.100255","url":null,"abstract":"<div><p>Advancements in sequencing technology, especially the emergence of next-generation sequencing (NGS), have immensely revolutionized the search for extremophilic microorganisms. The complementation of metagenomics and next-generation sequencing tools provides an unprecedented opportunity to explore hidden information about microbial diversity in extreme environments. NGS techniques allow us to understand the genomics, physiology, and evolution of extremophiles. Whole-genome/metagenome-based sequencing has revealed several mysteries of extremophiles that have enhanced our understanding of the evolution of extremophilic microbes. The present article attempts to provide extensive information on bacterial type extremophiles in the context of advanced NGS platforms.</p></div>","PeriodicalId":37938,"journal":{"name":"Ecological Genetics and Genomics","volume":"32 ","pages":"Article 100255"},"PeriodicalIF":0.0,"publicationDate":"2024-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141241769","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unveiling thymosin antimicrobial peptide from pearl spot, Etroplus suratensis: Molecular characterization, phylogenetic analysis, and functional implications","authors":"Kavya Gokul,&nbsp;Raniya Samad,&nbsp;Anne Maria Thomas,&nbsp;Swapna P. Antony","doi":"10.1016/j.egg.2024.100253","DOIUrl":"10.1016/j.egg.2024.100253","url":null,"abstract":"<div><h3>Background</h3><p>Antimicrobial peptides are small, cationic, amphipathic peptides composed of 10–50 amino acids, playing a crucial role in innate immunity. While they are found in both vertebrates and invertebrates, they are particularly effective in invertebrates. This study presents the identification and molecular characterization of the potential antimicrobial peptide thymosin from Pearl spot, <em>Etroplus suratensis</em> (<em>Es-Thyβ</em>). Phylogenetic analysis and structural characterization of the identified peptide are analyzed and substantiated through the study.</p></div><div><h3>Results</h3><p>The sequence analysis of <em>Es-Thyβ</em> revealed an Open Reading Frame (ORF) of 132 nucleotides, encoding a 43-amino acid peptide. The hydrophobicity of the peptide, determined using PepDraw, was found to be +68.93 Kcal*mol⁻¹. Through <em>in silico</em> analysis the identified Thymosin was determined to be <em>Es-Thyβ</em>. The structure and characteristics of the peptide sequence were further analyzed using multiple <em>in silico</em> software tools, providing confirmation of its identity as <em>Es-Thyβ.</em></p></div><div><h3>Conclusion</h3><p>The study identified and described a potential antimicrobial peptide <em>Es-Thyβ</em>, a member of the thymosin β4 family, with conserved sequences and evolutionary links across teleost fishes.</p></div>","PeriodicalId":37938,"journal":{"name":"Ecological Genetics and Genomics","volume":"31 ","pages":"Article 100253"},"PeriodicalIF":0.0,"publicationDate":"2024-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140786975","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Estimation of standard heterosis and heterotic grouping of elite sorghum [Sorghum bicolor (L) Moench] inbred lines under moisture stress conditions","authors":"Temesgen Begna ,&nbsp;Techale Birhan ,&nbsp;Taye Tadesse","doi":"10.1016/j.egg.2024.100242","DOIUrl":"10.1016/j.egg.2024.100242","url":null,"abstract":"<div><p>The development and selection of appropriate parents are prerequisites for sorghum hybrid variety development. The lack of a broad genetic base is the most significant constraint to sorghum crop improvement. The assignment of sorghum germplasm lines to appropriate heterotic groups is critical for increasing sorghum productivity. The experiment was conducted to determine the amount of standard heterosis and to categorize sorghum inbred lines into distinct heterotic groups. In the 2019 main cropping season, 42 sorghum genotypes were tested using an alpha-lattice design with two replications. Combined analysis of variance showed that there was a highly significant difference (p &lt; 0.01) among the genotypes for all studied traits. The maximum grain yield was obtained from the hybrids 4x14 (6.32 tha^-1), followed by the hybrids 8x15 (5.92 tha^-1), 1x15 (5.88 tha^-1), 13x14 (5.78 tha^-1) and 6x15 (5.57 tha^-1), with an average value of 5.0 tha^-1. Similarly, the 4x14 hybrid exhibited a maximum grain yield with 30.71 % heterosis, which was greater than the standard check (ESH-4) for grain yield. The two heterotic (A and B) groups were identified based on their specific combining ability effects, whereas three heterotic groups were identified based on their general combining ability effects to develop superior hybrids from broad base and suitable parents. Finally, based on yield performance, heterotic response and combining ability estimates for grain yield and its components, the hybrid crosses 4x14, 8x15, 1x15, 11x14, 11x15, 13x14, and 6x15 were found to be the most promising and potential hybrids that could be exploited commercially after critical evaluation for their superiority and yield stability across locations over the years.</p></div>","PeriodicalId":37938,"journal":{"name":"Ecological Genetics and Genomics","volume":"31 ","pages":"Article 100242"},"PeriodicalIF":0.0,"publicationDate":"2024-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140797005","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigation of post-immunization immune response gene expression kinetics in lymphoid tissues of White Leghorn and Indian native chicken","authors":"Laxmikant Sambhaji Kokate ,&nbsp;Sanjeev Kumar ,&nbsp;Ananta Kumar Das ,&nbsp;Abdul Rahim ,&nbsp;Jowel Debnath","doi":"10.1016/j.egg.2024.100251","DOIUrl":"10.1016/j.egg.2024.100251","url":null,"abstract":"<div><p>Understanding the basal expression kinetics of genes related to innate immunity aids effective genetic selection for immune response and how host genetics behaves with tolerance to Newcastle disease (ND) is obscure. It was hence aimed to investigate post-immunization mRNA expression kinetics of some immune response genes in White Leghorn, Aseel and Kadaknath chicken. Day-old chicks were vaccinated with ND RDF-1 strain with booster dose at 28-days of age at the experimental layer farm of ICAR-Central Avian Research Institute, Izatnagar (India) and lymphoid tissues were collected in RNAlater at 42-days of age. Total RNA was extracted and first strand cDNA was synthesized. Relative quantification of mRNA expression of IL1-β, IFN-γ, iNOS and TLR15 gene was assessed by qRT-PCR. The mRNA expressions significantly varied among the genes in spleen and thymus, and moderate expression of IFN-γ in all chicken genotypes suggested good protection against Newcastle disease virus. IL1-β-mRNA expressed at the highest level (P˂ 0.05) in spleen followed by thymus and bursa, whereas IFN-γ and iNOS expressed highly (P˂ 0.05) in thymus followed by spleen and bursa. In case of TLR15, the expression was significantly the highest in spleen followed by bursa and thymus. Kadaknath had the highest (P˂ 0.05) expression of IL1-β in thymus and that of iNOS in spleen, whereas Aseel showed the least (P &gt; 0.05) expression than Kadaknath and White Leghorn. The study revealed the existence of wide variation in basal expression levels of immune response genes among different lymphoid tissues and lack of discernible differences in the expression between the chicken genotypes.</p></div>","PeriodicalId":37938,"journal":{"name":"Ecological Genetics and Genomics","volume":"31 ","pages":"Article 100251"},"PeriodicalIF":0.0,"publicationDate":"2024-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140782120","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rootstock influences on growth and bearing precocity in juvenile cocoa","authors":"Esther Anokye,&nbsp;Ebenezer Obeng-Bio,&nbsp;Abraham Akpertey,&nbsp;Moses Kwame Aidoo,&nbsp;Margaret Saka Aduama-Larbi,&nbsp;Francis Kwame Padi","doi":"10.1016/j.egg.2024.100254","DOIUrl":"10.1016/j.egg.2024.100254","url":null,"abstract":"<div><p>Within the cocoa germplasm used for variety development in West Africa, knowledge of varieties that are suitable as rootstocks to promote growth and precocity of clones is lacking. Six cocoa varieties were used to generate 36 clones for evaluation. For each of the six scions selected as ortets from previous progeny trials, the corresponding seed-derived family was regenerated through manual pollinations. The scions were grafted in a full diallel fashion on each of the six seed-derived family, and field evaluated in four replications following a randomized complete block design. Also, seed-derived varieties were evaluated in the nursery for vigour characteristics, and under similar conditions as the 36 clones in the field. Genotype heterozygosity at 72 SNP loci was significantly correlated with vigour characteristics of the six varieties. Seed-derived varieties of high vigour benefited clone performance for vigour, and dry bean yield when used as rootstocks but not as scion. The maximum photochemical efficiency of photosystem II (Fv/Fm) was different among seed-derived varieties and clones in the dry season but not the rainy season. Among clones, higher Fv/Fm values were associated with higher-vigour rootstock varieties. Diallel grafting model enabled partitioning of total variance into several sub-components. The variance due to specific compatibility ability was not significant for any of the measured traits. Decomposition of the reciprocal variance revealed that significant reciprocal effects were mainly due to significant maternal (rootstock) effects. The key finding is that, for any scion, the most precocious clone is obtained by grafting on high vigour rootstock varieties.</p></div>","PeriodicalId":37938,"journal":{"name":"Ecological Genetics and Genomics","volume":"31 ","pages":"Article 100254"},"PeriodicalIF":0.0,"publicationDate":"2024-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140781796","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recent advances in the genetic parameters and prospects of cultivation of the climate-smart herb senna (Senna alexandrina Mill.): A significant industrial medicinal crop","authors":"A. Kumar,&nbsp;A.K. Gupta,&nbsp;R.K. Lal","doi":"10.1016/j.egg.2024.100248","DOIUrl":"https://doi.org/10.1016/j.egg.2024.100248","url":null,"abstract":"<div><p>The bulk of senna plant components are used to make herbal medications to treat infections and other ailments. Except for grading, more than 80% of dried leaves are exported from India. The current study examined the genetic variability, correlations, and character contributions of 25 senna accessions. The amplitude of the GCV was larger than that of the PCV, showing that the selected factors interact genetically. X1 had substantial and positive connections with X4, X5, X6, X8, X11, and X13, while X2 had significant and positive links with X7, X10, X11, X12, X13, and X14. The characteristic X11 made the largest direct contribution to the X14 attribute. In X1 and X13, the indirect effect was greater. Fresh and dry leaf weight had high heritability and medium GA over the mean, respectively, whereas pod weight had high heritability and GA over the mean, at 93.62 and 94.93%. The mean, heritability, and GA over the mean of genotypes Gen 1, 2, 3, 4, and 25 were selected for high yield in the most economically significant traits. As a result, the senna genotypes mentioned above may be effectively exploited.</p></div>","PeriodicalId":37938,"journal":{"name":"Ecological Genetics and Genomics","volume":"31 ","pages":"Article 100248"},"PeriodicalIF":0.0,"publicationDate":"2024-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140646609","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative resistance of wheat genotypes to aphid infestation under varied sowing dates","authors":"Muhammad Nawaz ,&nbsp;Irfanullah Khan ,&nbsp;Farman Ullah Dawar ,&nbsp;Ibni Amin Khalil ,&nbsp;Zahid Hussain ,&nbsp;Jan Saad ,&nbsp;Syed Majid Rasheed ,&nbsp;Khan Rahamdad","doi":"10.1016/j.egg.2024.100245","DOIUrl":"https://doi.org/10.1016/j.egg.2024.100245","url":null,"abstract":"<div><p>Aphid infestations pose a significant threat to wheat production worldwide, leading to substantial yield losses. Understanding the variation in wheat genotype response to aphids’ infestation across different sowing dates is crucial for effective insect pest management approaches. In this study, we investigated the relative resistance of various wheat genotypes to aphid infestations under varying sowing dates over multiple growing seasons 2019–20 and 2020–21 at Cereal Crops Research Institute, Pirsabak, Nowshera, Pakistan. The objectives were to assess the impact of sowing date on aphid infestations and to identify wheat genotypes that exhibit consistent resistance to aphids. The results revealed significant variations in aphid infestations across different sowing dates, with early-sown (October) wheat genotypes; Khaista-17 and PR-123 having 5.53 and 5.22 aphids/tiller, respectively, experiencing higher aphid pressure among all sowing dates, while lower aphid infestation 1.91 and 2.30 aphids/tiller observed on PR-125 and PR-127 respectively in late-sown (January). In contrast, Khaista-17 and PR-126 sown in October attained higher yield (4041 and 3639 kg ha⁻¹), while the same genotypes attained 448 and 441 kg ha⁻¹ respectively, that late-sown at January 2019–20. Similarly, these genotypes, Khaista-17 and PR-131 with 4.44 and 4.31 aphids/tiller not only sustained lower aphid populations but also demonstrated minimal yield loss due to aphid damage during 2020–21. This research highlights the importance of considering sowing date as a critical factor in wheat-aphid interactions. Furthermore, this study offers valuable insights for pest management strategies aimed at mitigating aphid-related yield losses and improve overall wheat production sustainability.</p></div>","PeriodicalId":37938,"journal":{"name":"Ecological Genetics and Genomics","volume":"31 ","pages":"Article 100245"},"PeriodicalIF":0.0,"publicationDate":"2024-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140646608","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Role of Reproductive Technologies and Cryopreservation of Genetic Materials in the Conservation of Animal Genetic Resources","authors":"Aweke Engdawork,&nbsp;Teklewold Belayhun,&nbsp;Tesfalem Aseged","doi":"10.1016/j.egg.2024.100250","DOIUrl":"https://doi.org/10.1016/j.egg.2024.100250","url":null,"abstract":"<div><p>The conservation of animal genetic resources guarantees the continual existence of diversified nature and thereby ensures the achievement of valuable economic, ecological, social, and cultural demands. Animal genetic resources can be conserved in their natural environments (in-situ conservation) and outside their natural habitats (ex-situ conservation) through different techniques such as zoo, captive breeding, aquarium, and in gene banks by collecting and preserving their germplasm. Reproductive technologies and cryopreservation of genetic materials play a pivotal role in preserving genetic diversity, supporting breeding programs, contributing to biodiversity conservation, adapting to changing environments, and fostering advancements in agriculture and animal husbandry. Its importance extends to ensuring food security, resilience in the face of climate change, and economic opportunities for communities. The collection and cryopreservation of genetic materials, including sperm, oocytes, embryos, and other biological materials capable of generating new offspring, have critical importance in supporting in-situ conservation efforts of threatened animals and the rehabilitation of extinct species and breeds. Advancements in reproductive technologies such as artificial insemination, in-vitro fertilization, embryo transfer, and nuclear transfer have greatly promoted efficiencies in animal reproduction and serve as a significant tool for domestic and wildlife animal conservation, particularly those threatened with extinction. Cryopreservation of germplasm and the application of reproductive technologies possess great success in breeding improvements and genetic resource conservation strategies, increasing the possibility of population rehabilitation. Good achievements have been observed in reconstituting animal populations in different parts of the world, while successful attempts have also been recorded in recovering extinct animals from cryopreserved genetic materials. In Ethiopia, reproductive technologies, such as cryopreservation and artificial insemination, are limited to the collection and distribution of bovine semen for genetic improvement and/or restocking activity. However, other reproductive technologies, including in vitro fertilization, ovulation induction, and reproductive surgeries, are not yet fully implemented due to various limitations. Initiative efforts are started in semen collection from indigenous cattle breeds, horses, and the endangered Ethiopian wolf for the conservation of the animal species or breed. Therefore, animal conservation strategies should adopt and benefit from advances in animal reproduction and cryopreservation technologies. Furthermore, proper emphasis and attention should be given to the development and use of cryopreservation and reproductive technologies in the conservation of animal genetic resources. Therefore, the purpose of this review is to emphasize the commonly utilized reproductive technologies","PeriodicalId":37938,"journal":{"name":"Ecological Genetics and Genomics","volume":"31 ","pages":"Article 100250"},"PeriodicalIF":0.0,"publicationDate":"2024-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140646882","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}