Visnik ukrains'kogo tovaristva genetikiv i selekcioneriv最新文献_第5页

Meiosis analysis in soft winter wheat varieties — carriers of wheat-rye translocations 1BL.1RS and 1AL.1RS 软质冬小麦品种减数分裂分析——小麦-黑麦易位的载体1BL。1RS和1al

Visnik ukrains'kogo tovaristva genetikiv i selekcioneriv Pub Date : 2019-03-01 DOI: 10.7124/VISNYK.UTGIS.16.2.1055

I. I. Lyalko, O. Dubrovna, B. Morgun

{"title":"Meiosis analysis in soft winter wheat varieties — carriers of wheat-rye translocations 1BL.1RS and 1AL.1RS","authors":"I. I. Lyalko, O. Dubrovna, B. Morgun","doi":"10.7124/VISNYK.UTGIS.16.2.1055","DOIUrl":"https://doi.org/10.7124/VISNYK.UTGIS.16.2.1055","url":null,"abstract":"Aim. Investigation of the features of the meiosis flow in winter wheat varieties — carriers wheat-rye translocations 1BL.1RS and 1AL.1RS. Methods. A comparative analysis of the meiosis flow in winter wheat varieties, the carriers of various translocations, was carried out using the method of temporary pressure preparations. Results. The frequency and spectrum of meiosis anomalies in introgressive winter wheat varieties with wheat-rye translocations 1BL.1RS and 1AL.1RS are established. The variability of the frequency of violations at different stages of microsporogenesis in the carrier classes of a certain translocation is shown, which may be due to a different period of stabilization of their genomes. It was found that the frequency of violations in varieties with different translocations did not exceed 10 %, which is normal for stable introgressive varieties. Conclusions. It was found that in all stages of microsporogenesis in the number of cells with violations and their spectrum, varieties with translocation of 1BL.1RS are slightly different from those with translocation of 1AL.1RS. Keywords: bread winter wheat, translocations 1BL.1RS and 1AL.1RS, meiotic flow.","PeriodicalId":379244,"journal":{"name":"Visnik ukrains'kogo tovaristva genetikiv i selekcioneriv","volume":"13 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132850488","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Introduction into in vitro culture and cytogenetic analysis of Iris attica Boiss. & Heldr. and Iris pseudopumila Tineo plants 鸢尾离体培养及细胞遗传学分析。& Heldr。和鸢尾属植物

Visnik ukrains'kogo tovaristva genetikiv i selekcioneriv Pub Date : 2019-03-01 DOI: 10.7124/visnyk.utgis.16.2.1058

M. O. Twardovska, I. Andreev, V. Kunakh

{"title":"Introduction into in vitro culture and cytogenetic analysis of Iris attica Boiss. & Heldr. and Iris pseudopumila Tineo plants","authors":"M. O. Twardovska, I. Andreev, V. Kunakh","doi":"10.7124/visnyk.utgis.16.2.1058","DOIUrl":"https://doi.org/10.7124/visnyk.utgis.16.2.1058","url":null,"abstract":"Aim. The work was aimed at the development of conditions for introduction into in vitro culture of two species of irises, Iris attica and I. pseudopumila to obtain aseptic seedlings with subsequent reintroduction into natural environment, as well as at cytogenetic analysis of the obtained plants. Methods. In vitro seed germination and seedling cultivation. Cytogenetic analysis of cells of root meristem, determination of chromosome number and morphology in mitotic metaphase plates, anaphase analysis. Results. The plants of I. attica and I. pseudopumila were introduced in vitro. Aseptic seedlings were obtained, which were actively growing on MS/2 medium without phytohormones. The experiments on the adaptation of the plants to greenhouse conditions revealed the high survival rate for both species. The chromosome number 2n = 16 was established for the obtained plants of both I. attica and I. pseudopumila. Mixoploidy was detected in root meristem of some of the plants, the incidence of which was 10.9 % for I. pseudopumila and 30.4 % for I. attica. The frequency of cells with chromosomal rearrangements revealed by anaphase analysis in root meristem of I. pseudopumila seedlings was 2.6 %; in I. attica plants, chromosome aberrations were not detected. Conclusions. The plants of I. attica and I. pseudopumila were introduced into in vitro culture, aseptic seedlings were obtained, which showed a high survival level when adapted to greenhouse conditions. Chromosome number 2n = 16 was established for the obtained plants of both species. The root apical meristems of the seedlings were found to be mixoploid, with the incidence of mixoploidy in I. attica identified as three times higher than in I. pseudopumila plants.Keywords: I. attica Boiss. & Heldr., I. pseudopumila Tineo, aseptic seedlings, mixoploidy, anaphase aberration.","PeriodicalId":379244,"journal":{"name":"Visnik ukrains'kogo tovaristva genetikiv i selekcioneriv","volume":"211 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132162536","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genetic-epidemiological study of pediatric population of Krasnohrad district of Kharkiv region 哈尔科夫州克拉斯诺哈拉德地区儿童遗传流行病学研究

Visnik ukrains'kogo tovaristva genetikiv i selekcioneriv Pub Date : 2018-09-07 DOI: 10.7124/VISNYK.UTGIS.16.1.902

O. Fedota, Y. O. Sadovnychenko, N. Movchan, O. V. Kolodiazhnyi, R. S. Dolzhenkova, L. Roshcheniuk, I. M. Kasian

{"title":"Genetic-epidemiological study of pediatric population of Krasnohrad district of Kharkiv region","authors":"O. Fedota, Y. O. Sadovnychenko, N. Movchan, O. V. Kolodiazhnyi, R. S. Dolzhenkova, L. Roshcheniuk, I. M. Kasian","doi":"10.7124/VISNYK.UTGIS.16.1.902","DOIUrl":"https://doi.org/10.7124/VISNYK.UTGIS.16.1.902","url":null,"abstract":"Aim. Study of genetic pathology burden, both monogenic and chromosomal, of pediatric population of Krasnohrad district of Kharkiv region. Methods. Epidemiological and clinical-genealogical methods have been used. The information on 252 patients aged 0– 17 years from all major sources of their registration has been analyzed. Results. The monogenic pathology burden of pediatric population was 0.28 %, chromosomal disorders burden — 0.13 %, 0.41 % in total. There were 14 nosological forms of single-gene diseases, the most common ones were sensorineural hearing loss (1:1295), congenital glaucoma (1:3884), congenital hypothyroidism (1:3884), ichthyosis (1:3884). For the past ten years there was a change in the spectrum of single-gene pathology in pediatric population of Krasnohrad district. Chromosomal abnormalities in the area were represented by Down syndrome (1:868) and Prader-Willi syndrome (1:7767). Conclusions. The genetic pathology burden of the pediatric population of the Krasnohrad district of Kharkiv region, the spectrum of monogenic and chromosomal pathology, and prevalence of their individual nosological forms are comparable to those in most European countries. Keywords: genetic pathology, prevalence, single-gene diseases, chromosomal disorders, Kharkiv region.","PeriodicalId":379244,"journal":{"name":"Visnik ukrains'kogo tovaristva genetikiv i selekcioneriv","volume":"297 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121376397","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Intron length polymorphism of beta-tubulin genes in Ulmus pumila L. plants in the Steppe Prydniprov'ia 普雷德尼普罗夫草原矮榆-微管蛋白基因内含子长度多态性

Visnik ukrains'kogo tovaristva genetikiv i selekcioneriv Pub Date : 2018-09-07 DOI: 10.7124/visnyk.utgis.16.1.900

Y. Pirko, L. Kalafat, N. Pirko, A. N. Rabokon, S. Privalikhin, A. Demkovych, Yu. O. Bilonozhko, O. Kravets, A. Alexeyeva, N. Khromykh, Y. Lykholat

{"title":"Intron length polymorphism of beta-tubulin genes in Ulmus pumila L. plants in the Steppe Prydniprov'ia","authors":"Y. Pirko, L. Kalafat, N. Pirko, A. N. Rabokon, S. Privalikhin, A. Demkovych, Yu. O. Bilonozhko, O. Kravets, A. Alexeyeva, N. Khromykh, Y. Lykholat","doi":"10.7124/visnyk.utgis.16.1.900","DOIUrl":"https://doi.org/10.7124/visnyk.utgis.16.1.900","url":null,"abstract":"Aim. Using DNA markers related to the genes encoding β-tubulin in plants, to evaluate the intraspecific genetic polymorphism of Ulmus pumila L. in the Steppe Prydniprov'yia and compare it with the polymorphism of this species within the natural range. Method. Analysis of the intron length of polymorphism of β-tubulin genes (TBPmethod). Results. It was established that the plants differ from each other slightly in terms of the number of identified amplicons and the nature of their distribution when comparing electrophoretic profiles obtained on the basis of the TBP analysis for U. pumila. Half of the fragments found in the samples are rare. The average number of fragments (alleles) on the locus (Ne), Shannon information index (I) and polymorphism information content value (PIC) amounted respectively: 1.26, 0.27, 0.21, and were lower than in natural populations, analyzed using microsatellite markers. Conclusions. U. pumila plants growing in the Steppe Prydniprov'yi for the TBP markers have a lower level of genetic diversity than was found during the analysis of natural populations using other molecular markers. Among the possible reasons may be the nature (methodology) of the creation and age of the tree stands examined, as well as the nature of the genetic markers used to analyze the genetic polymorphism of the species.Keywords: TBP-method, introns, β-tubulin, Ulmus pumila, genetic diversity.","PeriodicalId":379244,"journal":{"name":"Visnik ukrains'kogo tovaristva genetikiv i selekcioneriv","volume":"2 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126291121","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Evolution of Darwinism. The classical stage: 1859 — 1926 years 达尔文主义的进化。古典阶段:1859 - 1926年

Visnik ukrains'kogo tovaristva genetikiv i selekcioneriv Pub Date : 2018-09-07 DOI: 10.7124/visnyk.utgis.16.1.904

Y. V. Vagyn

引用次数: 0

Expression analisis of the ITSN2 and TKS5 mRNA isoforms in human malignant breast tumors 人乳腺恶性肿瘤中ITSN2和TKS5 mRNA亚型的表达分析

Visnik ukrains'kogo tovaristva genetikiv i selekcioneriv Pub Date : 2018-09-07 DOI: 10.7124/visnyk.utgis.16.1.899

S. Kropyvko, L. Tsyba, O. Novokhatska, L. Syvak, T. Tarasenko, A. Grabovoy, A. Rynditch

引用次数: 0

Adhesion proteins are able to controle the proliferation and size of neonatal cardiomoycytes in Mus musculus 黏附蛋白能够控制小家鼠新生心肌细胞的增殖和大小

Visnik ukrains'kogo tovaristva genetikiv i selekcioneriv Pub Date : 2018-09-07 DOI: 10.7124/VISNYK.UTGIS.16.1.897

V. Balatskyi, L. Macewicz, T. Ruban, O. Piven

引用次数: 0

Molecular organization of 5S rDNA of Brunfelsia uniflora (Pohl.) D. Don 单花褐藻5S rDNA的分子结构d .不

Visnik ukrains'kogo tovaristva genetikiv i selekcioneriv Pub Date : 2018-09-07 DOI: 10.7124/VISNYK.UTGIS.16.1.903

A. Y. Shelyfist, Y. Tynkevich, R. Volkov

{"title":"Molecular organization of 5S rDNA of Brunfelsia uniflora (Pohl.) D. Don","authors":"A. Y. Shelyfist, Y. Tynkevich, R. Volkov","doi":"10.7124/VISNYK.UTGIS.16.1.903","DOIUrl":"https://doi.org/10.7124/VISNYK.UTGIS.16.1.903","url":null,"abstract":"Aim. The 5S rDNA represents a convenient model for studying of the molecular evolution of tandemly arranged repeated sequences. However, in many groups of angiosperms this genomic region still remains poorly studied. So far, in the family Solanaceae the 5S rDNA was described only for five genera. In order to elucidate the 5S rDNA organization in representatives of other genera of Solanaceae, we decided to explore organization of this region in Brunfelsia uniflora (tribe Petunieae). Methods. PCR amplification, cloning and sequencing of 5S rDNA. Results. Three clones of 5S rDNA of Brunfelsia uniflora were sequenced. It was found that only one class of repeats ranging in length from 343 to 347 bp is present in the genome of B. uniflora. The level of intragenomic similarity for the 5S rDNA intergeneric spacer regions (IGS) ranges from 86.7 to 96.4 %. The IGSs of B. uniflora and of other members of Solanaceae family differ significantly: the sequence homology was detected only for sequence motives required for RNA polymerase III transcription initiation and termination. Conclusions. The molecular evolution of the 5S rDNA IGS occurs at a high rate in the Solanaceae family. Accordingly, the comparison of the IGS should be used to clarify the phylogenetic relationship between taxa of low rank, in particular between species and genera of the tribe Petunieae. Keywords: 5S rDNA, intergeneric spacer, molecular evolution, Brunfelsia, Solanaceae.","PeriodicalId":379244,"journal":{"name":"Visnik ukrains'kogo tovaristva genetikiv i selekcioneriv","volume":"271 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116248259","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Determination of the reference values of amino acids and acylcarnitines level in the newborn dry blood spots in Ukraine 乌克兰新生儿干血斑中氨基酸和酰基肉碱水平参考值的测定

Visnik ukrains'kogo tovaristva genetikiv i selekcioneriv Pub Date : 2018-09-07 DOI: 10.7124/VISNYK.UTGIS.16.1.898

O. Barvinska, N. Olkhovych, N. Gorovenko

{"title":"Determination of the reference values of amino acids and acylcarnitines level in the newborn dry blood spots in Ukraine","authors":"O. Barvinska, N. Olkhovych, N. Gorovenko","doi":"10.7124/VISNYK.UTGIS.16.1.898","DOIUrl":"https://doi.org/10.7124/VISNYK.UTGIS.16.1.898","url":null,"abstract":"Aim. Implementation of neonatal screening of the group of severe inherited disorders of amino acids, fatty and organic acids metabolism in Ukraine involves determination of the biological variation of amino acids and acylcarnitines level in the blood that depends on the gender, geographical origin of the samples and the gestation term of newborn. Methods. The main method of rapid and reliable diagnosis of this group of inherited disorders is determination of amino acids and acylcarnitines level by liquid chromatography tandem mass spectrometry. Results. Reference intervals of amino acids and acylcarnitines concentration in dry blood samples of newborns from different regions of Ukraine haven’t differed. However, it was found necessity to use separate reference intervals of some amino acids and acylcarnitines (citrulline, arginine, methionine, leucine, tyrosine, alanine, histidine, C0, C3, C3DC, C4, C5, C6, C8, C8:1, C10:2, C12, C14:1, C16, C18OH, C18:1OH) for premature and full-term newborns. Also, it was revealed that for male and female newborns it is necessary to use different reference intervals of citrulline, leucine, C8:1, arginine, methionine, C5, C8. Conclusions. In this study it was found that variation of amino acids and acylcarnitines concentration in dry blood spots depends on the newborns gestation term and sex. We have proposed a two-stage procedure of biomarkers interpretation for which the values variation in different neonatal groups was detected. Keywords: amino acids and acylcarnitines reference intervals, inherited disorders of amino acids, fatty and organic acids metabolism, newborn screening.","PeriodicalId":379244,"journal":{"name":"Visnik ukrains'kogo tovaristva genetikiv i selekcioneriv","volume":"42 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130640882","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Genetic-physiological basis of legume crops resistance to drought stress 豆科作物抗旱的遗传生理基础

Visnik ukrains'kogo tovaristva genetikiv i selekcioneriv Pub Date : 2018-09-07 DOI: 10.7124/VISNYK.UTGIS.16.1.901

V. Sichkar, S. Pasichnyk

{"title":"Genetic-physiological basis of legume crops resistance to drought stress","authors":"V. Sichkar, S. Pasichnyk","doi":"10.7124/VISNYK.UTGIS.16.1.901","DOIUrl":"https://doi.org/10.7124/VISNYK.UTGIS.16.1.901","url":null,"abstract":"Goal. Identify the effective criteria for drought tolerances of leguminous crops, based on their basis evaluate the recommendation for cultivating soybean cultivars and discuss the mechanisms of resistance on the organism and molecular levels. Methods. The collection and breeding genotypes of soybean, chickpea and pea were grown in field and laboratory experiments as well as cultivars included in the State register of plant varieties suitable for dissemination in Ukraine. The reaction of soybean plants on the effect of water stress was determined in climatic chambers at the temperature of 30–32 °C. Results. The genetic variability in absorption of water by seeds of various soybean cultivars was detected especially at the initial stages of soaking. The tolerance to drought is associated with the level of free proline accumulation and water-keeping proteins in the leaves, the area of the leaf surface, the loss of moisture by the plant for a certain period, the development of the root system. Soybean cultivars Arcadia odesskaya and Hodson distinguished by increased resistance to drought. Conclusions. Tested in the field and laboratory conditions methods for determining resistance to water stress may be recommended for use in breeding research with agricultural crops. Keywords: breeding of leguminous crops, drought tolerance, adaptability to high temperature.","PeriodicalId":379244,"journal":{"name":"Visnik ukrains'kogo tovaristva genetikiv i selekcioneriv","volume":"27 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133098564","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1