Visnik ukrains'kogo tovaristva genetikiv i selekcioneriv最新文献

{"title":"Academician of the National Academy of Sciences of Ukraine Sybirny Andriy Andriyovych – a scientist, teacher, public figure","authors":"M. L. Bars’ka, D. V. Fedorovych","doi":"10.7124/visnyk.utgis.21.1-2.1600","DOIUrl":"https://doi.org/10.7124/visnyk.utgis.21.1-2.1600","url":null,"abstract":"","PeriodicalId":379244,"journal":{"name":"Visnik ukrains'kogo tovaristva genetikiv i selekcioneriv","volume":"8 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140508421","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Properties of lectin from tissue culture of Ungernia victoris and its ultrasonic extraction","authors":"I. S. Karpova, I. Konvalyuk, L. Mozhylevska, V. Lylo, V. A. Kunakh","doi":"10.7124/visnyk.utgis.21.1-2.1597","DOIUrl":"https://doi.org/10.7124/visnyk.utgis.21.1-2.1597","url":null,"abstract":"The aim was to investigate biomass extracts of U. victoris strain UV-2 tissue culture for the content of lectins, to study their general characteristics and to optimize the method of ultrasonic extraction to obtain a soluble form of the lectin. Methods. Tissue culture method, direct hemagglutination test for detection of lectin activity (LA), determination of lectin carbohydrate specificity, ultrasonic extraction method. The results. Lectin activity was detected in the soluble (supernatant) and insoluble (sediment) fractions of the extract of biomass U. victoris tissue culture. In the soluble fraction LA was significantly less compared to that in the sediment, which indicated the presence of the membrane-bound form of the lectin. LA was characterized by pronounced species specificity: the reaction with mouse erythrocytes was the most intense. The study of carbohydrate specificity revealed a weak affinity for monosugars (galactose and galactosamine) and pronounced suppression of LA in the case of polysaccharides - hyaluronic acid, heparin and mucin. The possibilities of the ultrasound extraction method for separation the detected lectin from the the cell surface in the sediment fraction were investigated and the extraction procedure was optimized. The transition of LA into the soluble phase under the influence of ultrasound depended on the following parameters: the concentration and volume of the extract, as well as the time of exposure to ultrasound. It was established that the LA of the soluble phase doubled compared to the initial one with an extract concentration of 50 mg/ml and a volume of 20 ml under the action of ultrasound 40 kHz/70 W in the interval of 15-45 minutes. Longer ultrasound treatment had a negative effect on LA. Conclusions. Lectin activity of U. victoris tissue culture biomass extracts was discovered for the first time, which can become a promising source of lectin with a wide spectrum of pharmacological properties. The general characteristics of the lectin were given, and its species and carbohydrate specificity was revealed. The lectin was generally characterized and its species and carbohydrate specificity were established. Possibilities of ultrasound extraction were used and the procedure was optimized to obtain a soluble form of the lectin, necessary for its further fundamental and practical research.","PeriodicalId":379244,"journal":{"name":"Visnik ukrains'kogo tovaristva genetikiv i selekcioneriv","volume":"56 6","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140507055","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The use of the 5S rDNA IGS region for DNA barcoding and molecular taxonomy of two Ukrainian species of the genus Tulipa L.","authors":"Y. Tynkevich, N. Roshka, E. E. Timkanych, I. Moysiyenko, R. A. Volkov","doi":"10.7124/visnyk.utgis.21.1-2.1596","DOIUrl":"https://doi.org/10.7124/visnyk.utgis.21.1-2.1596","url":null,"abstract":"Taxonomic identification of closely related representatives of the genus Tulipa L. is complicated by the convergent similarity of morphological characters, interspecific hybridization, polyploidy and asexual reproduction. Among the tulips in the flora of Ukraine, the most problematic is the status of taxa of the complex species T. sylvestris L. Aim. To examine the organization and evolution of the nuclear 5S rDNA IGS region for four closely related Tulipa taxa and to evaluate the possibility of its use in the molecular taxonomy of this plant group. Methods. PCR amplification, cloning and sequencing of 5S rDNA repeat units, bioinformatic and phylogenetic analysis. Results. In this work, 5S rDNA repeats were sequenced for the species T. graniticola and T. ophiophylla, which are considered to be synonyms of T. sylvestris L. by many authors. We also assembled the main ribotypes from full-genome libraries of short Illumina reads for T. patens, and T. saxatilis, the species related to T. sylvestris. A comparative analysis of the structure of 5S rDNA sequences and a phylogenetic analysis based on the IGS region were carried out. Conclusions. A high level of 5S rDNA IGS variability has been demonstrated for representatives of the subgenus Eriostemones of the genus Tulipa. The use of 5S rDNA IGS for phylogenetic analysis enabled the clear delimitation of the closely related species T. saxatilis, T. patens, and the complex species T. sylvestris, as well as the delineation of the taxa with the controversial status, T. graniticola and T. ophiophylla. Therefore, the use of this region of the nuclear genome is a promising approach to the molecular taxonomy of the genus Tulipa.","PeriodicalId":379244,"journal":{"name":"Visnik ukrains'kogo tovaristva genetikiv i selekcioneriv","volume":"57 8","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140507032","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"XVIII International scientific conference «Factors in experimental evolution of organisms», dedicated to the 50th anniversary of the Institute of Molecular Biology and Genetics of NAS of Ukraine","authors":"I. Konvalyuk, M. O. Twardovska, A. Holubenko, N. Drobyk, V. A. Kunakh","doi":"10.7124/visnyk.utgis.21.1-2.1603","DOIUrl":"https://doi.org/10.7124/visnyk.utgis.21.1-2.1603","url":null,"abstract":"","PeriodicalId":379244,"journal":{"name":"Visnik ukrains'kogo tovaristva genetikiv i selekcioneriv","volume":"57 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140507290","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"VII International scientific and practical conference Ternopil Bioscience – 2023","authors":"M. Z. Prokopiak, О. І. Bodnar, N. Drobyk","doi":"10.7124/visnyk.utgis.21.1-2.1604","DOIUrl":"https://doi.org/10.7124/visnyk.utgis.21.1-2.1604","url":null,"abstract":"The article summarizes the results of the VII International Scientific and Practical Conference Ternopil Bioscience – 2023, dedicated to the 100th anniversary of the birth of the renowned botanist, taxonomist, and florist, Ph.D., Associate Professor, Head of the Department of Botany Valentina Omelianivna Shymanska. The event took place from May 11 to 13, 2023, in Ternopil at the Ternopil Volodymyr Hnatiuk National Pedagogical University. The conference covered the following thematic areas: experimental botany and plant physiology, biology and ecology of animals, microbiology and virology, cytogenetics and histomorphology, human anatomy and physiology, biochemistry and molecular biology, genetics of microorganisms, plants, animals, and humans, hydrobiology, biotechnology in plant cultivation, animal husbandry and veterinary medicine, environmental protection and biological aspects of biodiversity reproduction, applied aspects of adaptation of organisms, ecological biotechnology, and rational nature management, the impact of natural and social factors on human health, biomedicine and pharmacognosy, agronomy, plant protection, and quarantine, the history of biology, and the methodology of teaching natural sciences. A collection of abstracts was published before the conference opening. Over 102 participants took part in the conference. There were 8 plenary and 38 sectional reports. Following the discussions, it was decided to schedule and conduct the next conference in the Spring of 2024.","PeriodicalId":379244,"journal":{"name":"Visnik ukrains'kogo tovaristva genetikiv i selekcioneriv","volume":"21 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140506884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Some moments in the history of the Institute of Molecular Biology and Genetics of the National Academy of Sciences of Ukraine (to the 50th anniversary of the foundation)","authors":"V. A. Kunakh","doi":"10.7124/visnyk.utgis.21.1-2.1599","DOIUrl":"https://doi.org/10.7124/visnyk.utgis.21.1-2.1599","url":null,"abstract":"The article briefly reviews the background and history of the creation of the Institute of Molecular Biology and Genetics of the National Academy of Sciences of Ukraine (IMBG), organized in July 1973, in particular the state and trends of the development of genetics and molecular biology in Ukraine in the 1960s and 1970s and further in the IMBG until 2020s Some events that preceded the creation of the IMBG, the stages of the formation of new scientific directions, the creation of scientific departments and laboratories, the main scientific achievements and achievements over the 50 years of the institute's existence are analyzed. Special attention is paid to various stages of formation and development of scientific departments, achievements of individual outstanding scientists of the institute, role of IMBG in the development of molecular biology, genetics and biotechnology.","PeriodicalId":379244,"journal":{"name":"Visnik ukrains'kogo tovaristva genetikiv i selekcioneriv","volume":"5 4","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140508524","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"On the 50th anniversary of the Institute of Molecular Biology and Genetics of NAS of Ukraine","authors":"V. A. Kunakh","doi":"10.7124/visnyk.utgis.21.1-2.1595","DOIUrl":"https://doi.org/10.7124/visnyk.utgis.21.1-2.1595","url":null,"abstract":"","PeriodicalId":379244,"journal":{"name":"Visnik ukrains'kogo tovaristva genetikiv i selekcioneriv","volume":"1 11","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140506705","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evolution of the MGMT gene in primates: from structure to mobile genetic elements","authors":"O. Pidpala, L. Lukash","doi":"10.7124/visnyk.utgis.21.1-2.1598","DOIUrl":"https://doi.org/10.7124/visnyk.utgis.21.1-2.1598","url":null,"abstract":"The article collects data on the evolution of the reparative MGMT gene in primates and traces the evolutionary fate of a number of mobile genetic elements (MGEs). It was concluded that the evolution of the MGMT gene in primates underwent various changes from acquisition to loss of exon/intron sequences. As for primate-specific MGEs, they have a different evolutionary history and path - from the formation of species/genus-specific sequences to deletional degradation and can be components not only of intronic or exonic sequences, but also components of exons in the form of fragmented sequences and not be identified as MGEs.","PeriodicalId":379244,"journal":{"name":"Visnik ukrains'kogo tovaristva genetikiv i selekcioneriv","volume":"26 1-2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-01-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140507234","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biological section «The importance of G. Gamow’s ideas for biology of the 21st century»\" of the ХХІI International Gamov School Conference at Odesa I.I. Mechnikov National University","authors":"S. Chebotar","doi":"10.7124/visnyk.utgis.20.1-2.1523","DOIUrl":"https://doi.org/10.7124/visnyk.utgis.20.1-2.1523","url":null,"abstract":"","PeriodicalId":379244,"journal":{"name":"Visnik ukrains'kogo tovaristva genetikiv i selekcioneriv","volume":"42 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134413378","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"International scientific-practical conference “Theory and practice of improvement of farmed animals genetic resources”, dedicated to the celebration of the 30th anniversary of independence of Ukraine and the 110th anniversary of the birth of Igor Smirnov","authors":"O. Shcherbak, S. Kovtun, O. Lyzohub, I. Liuta","doi":"10.7124/visnyk.utgis.20.1-2.1522","DOIUrl":"https://doi.org/10.7124/visnyk.utgis.20.1-2.1522","url":null,"abstract":"","PeriodicalId":379244,"journal":{"name":"Visnik ukrains'kogo tovaristva genetikiv i selekcioneriv","volume":"11 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131037462","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}