Visnik ukrains'kogo tovaristva genetikiv i selekcioneriv最新文献

{"title":"Preliminary estimates of the number and diversity of the culturable endophytic bacteria from Deschampsia antarctica and Colobanthus quitensis","authors":"I. Prekrasna, A. Dzhulai, I. Parnikoza","doi":"10.7124/visnyk.utgis.19.1-2.1437","DOIUrl":"https://doi.org/10.7124/visnyk.utgis.19.1-2.1437","url":null,"abstract":"Endophytes are able to promote the plant's growth and are essential for their hosts to overcome biotic and abiotic stress. Plant-promoting capacities of these microorganisms can be crucial for Antarctic plants. The aim of the study was to estimate the number and diversity of culturable endophytic microorganisms from Deschampsia antarctica and Colobanthus quitensis growing in different localities of the West Antarctic Peninsula. Methods. Serial dilutions of the surface-sterilized plant biomass were inoculated on the CASO (Merk, USA) and R2A (Merk, USA) media and cultured at room temperature for a week. Number of colonies and their morphotypes were estimated. Results. The number of colony-forming units in the aboveground part and roots of D. antarctica was 4×106 ±2×106 and 7×106 ±2×106 per g of biomass, respectively. The colony forming units CFU number in the aboveground part of C. quitensis was 3×106±1×106 per g of biomass. The highest number of CFU was in the roots of D. antarctica from Galindez Island on both media (n×107). The highest CFU number in C. quitensis was in plants from Deception Island on nutrient-poor (7×106) and -rich (1×107) media. The lowest value was found for C. quitensis from Cape Pérez (7×103 on R2A and 1×104 on CASO). There was no significant difference in the number of CFU grown on nutrient-poor and nutrient-rich medium, but the morphology of the CFU varied on the two media. 112 pure cultures of endophytes were isolated. The vast majority (78%) of the isolates were gram-negative rods. The number of cultured endophytes of Antarctic vascular plans varied across the samples, which can be affected both by the features of the individual plants and the ecology of sites where they grow. Bacterial communities did not significantly vary in number depending on the medium but did somewhat differ in morphology. A collection of 112 endophyte isolates was developed, which is important to study their genetic and physiological traits and mechanisms of plant-bacteria interaction. Conclusions. Isolation of the endophytic microorganisms is important to study their genetic and physiological traits and mechanisms of plant-bacteria interaction.Keywords: Antarctic hairgrass, Antarctic pearlwort, maritime Antarctica, symbionts of plants","PeriodicalId":379244,"journal":{"name":"Visnik ukrains'kogo tovaristva genetikiv i selekcioneriv","volume":"22 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121715711","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biogenic elements in terrestrial substrates, freshwater pools and subice seamples of the Argentine islands – Kyiv peninsula region, the Maritime Antarctic","authors":"O. Yarovyi, H. Yevchun, D. Pishniak, I. Parnikoza","doi":"10.7124/visnyk.utgis.19.1-2.1440","DOIUrl":"https://doi.org/10.7124/visnyk.utgis.19.1-2.1440","url":null,"abstract":"Aim. The aim was to estimate inflow of nitrogen and phosphorus to the terrestrial substrate, freshwater pools, as well as in the ice core and subglacial waters, from the sea-bird colonies, and locations of non-colonial flying birds, or individual colonial birds activity. Methods. The samples of substrates, fresh waters, ice cores and samples from the subglacial waters collected during the 25th (2019/20) and 26th (2020/21) Ukrainian Antarctic expeditions in the area of the Argentine Islands – Kyiv Peninsula were analyzed by selected indicators of ornithogenic impact – concentration of bioaviable forms of nitrogen and phosphorus. Results. The concentration for nitrogen and phosphorus are shown to be ornitogenic in samples from all explored locations. High concentrations of ornithogenic compounds were detected not only inflowing from birds colonies, in particular penguins, but also in samples without their influence. Conclusions. The obtained data confirm the high level of influence of both colonial and non-colonial birds on terrestrial and freshwater ecosystems of the Argentine Islands-Kyiv Peninsula. It is shown that the edifying effect and inflow of significant amounts of nitrogen and phosphorus occurs not only in large penguin colonies, but also in single nesting or feeding and resting areas of non-colonial flying birds, or in the presence of individual colonial birds in the study area. However, in the locations of large penguin colonies, easily disolved bioavailable forms of nitrogen and phosphorus are found in much higher, hypertrophic concentrations. Relatively low concentrations of nutrients are currently found only in areas that have recently been released from the glacier and remain uninhabited, unattractive or inaccessible to birds. Such locations are currently marked only on the coast of the Kyiv Peninsula and are associated with the recent retreat of local glaciers. In case of the presence of penguins or the activity of non-colonial or individual colonial birds, all studied freshwater pools were eutrophied to varying extend. The dynamics of organic matter inflow to some of the studied freshwater pools of the region probably indicates the permanent eutrophication. Studies of the ice core of the Galindez Island Glacier and subglacial freshwaters  have shown that ornithogenic effects have been observed here for a long time.Key words: eutrophication, seabirds, the Antarctic, substrates, freshwater pools.","PeriodicalId":379244,"journal":{"name":"Visnik ukrains'kogo tovaristva genetikiv i selekcioneriv","volume":"99 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124689202","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fragments of different origins mobile genetic elements in the genome of coronavirus SARS-CoV-2","authors":"O. V. Pidpala, L. Lukash","doi":"10.7124/visnyk.utgis.19.1-2.1434","DOIUrl":"https://doi.org/10.7124/visnyk.utgis.19.1-2.1434","url":null,"abstract":"Aims. To analyze the presence of fragments of mobile genetic elements (MGE) of pro- and eukaryotic origin in the nucleotide sequence of the new human coronavirus SARS-CoV-2. Methods. The homology between nucleotide sequences was determined by BLAST 2.6.1. The results of the search and identification of MGE were performed using the ISfinder and CENSOR programs. Results. In the genome of the human coronavirus SARS-CoV-2, fragments of 11 bacterial IS elements (0.68% of the viral genome) and 23 eukaryotic MGEs (4.6%) have been identified. Of the 11 SARS-CoV-2 genes, fragments of IS-elements are present in two genes (ORF1ab and M) and in 3’UTR. Fragments of MGE eukaryotes have been detected within four genes (ORF1ab, S, N, and ORF7b) and in 3’UTR. The highest percentage of MGE was found in the ORF7b gene and in 3’UTR. Analyzing the insertion profiles of IS fragments on the example of known human coronaviruses and their intermediate hosts, we have been concluded that the possibility of using fragments of prokaryotic MGE for phylogenetic studies of the new human coronavirus SARS-CoV-2. Conclusions. Based on the obtained results, we have been suggested that fragments of MGE of pro- and eukaryotic origin may play a role in the evolution of the SARS-CoV-2 genome, in particular in the formation of viral genes. They could also be informative phylogenetic markers.Keywords: human coronaviruses, SARS-CoV-2, MGE, IS-elements, phylogenetic markers","PeriodicalId":379244,"journal":{"name":"Visnik ukrains'kogo tovaristva genetikiv i selekcioneriv","volume":"35 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116805852","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Organization and polymorphysm of 5S rDNA intergenic spacer of blackthorn (Prunus spinosa L.)","authors":"Y. Tynkevich, L. Kozub, R. Volkov","doi":"10.7124/visnyk.utgis.19.1-2.1439","DOIUrl":"https://doi.org/10.7124/visnyk.utgis.19.1-2.1439","url":null,"abstract":"Aim. The 5S rDNA repeated units consist of conserved regions encoding 5S rRNA and variable intergenic spacers (IGS). The IGS sequences are commonly used as molecular markers for low-ranking phylogenetic, phylogeographical and microevolutionary studies. However, this genomic region still remains undescribed for the waist majority of genera in the Rosaceae family. Here we present the first report of the IGS molecular organization and polymorphism for the widespread member of the Rosaceae family, Prunus spinosa. Methods. PCR amplification, cloning and sequencing of 5S rDNA, bioinformatics analysis. Results. The 5SrDNA IGS of P. spinosa was cloned sequenced and compared with the IGS of three other representatives of the genus Prunus. It was found that the IGS variants present in the genome of P. spinosa differ by the number of subrepeats located at the 5’end. Each of these subrepeats contain RNA Pol III transcription termination signal. The IGS sequences of Prunus species evolved mainly by accumulation of nucleotide substitutions. The level of intragenomic similarity of P. spinosa IGS is 96.5-100%, while the similarity between the IGS of different Prunus species ranges from 73.0 to 87.3%. Conclusions. The IGS of 5S rDNA represents a useful marker for phylogenetic and taxonomic studies in the genus Prunus.Keywords: 5S rDNA, molecular evolution, Prunus spinosa.","PeriodicalId":379244,"journal":{"name":"Visnik ukrains'kogo tovaristva genetikiv i selekcioneriv","volume":"13 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122158589","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimization of the fusion protein rhIL7-BAPmut renaturation process from the Escherichia coli inclusion bodies and its practical application","authors":"M. Usenko, O. Okunev, K. Bentsionova, O. Gorbatiuk, D. M. Irodov, M. Kovalchuk, V. Kordium","doi":"10.7124/visnyk.utgis.17.1.1199","DOIUrl":"https://doi.org/10.7124/visnyk.utgis.17.1.1199","url":null,"abstract":"Aim. The aim of our work was to optimize the renaturation method of the rhIL7-BAPmut fusion protein based on recombinant human interleukin-7 (rhIL7) and bacterial alkaline phosphatase with enhanced catalytic properties (BAPmut) for its obtaining in functionally active form. Methods. The cells of E. coli strain BL21(DE3) were transformed with pET24-IL7-BAPmut plasmid vector. Protein synthesis was induced by autoinduction protocol. Immobilized-metal affinity chromatography (IMAС) and slow dilution methods were applied for rhIL7-BAPmut fusion protein renaturation from bacterial inclusion bodies in vitro. Results. Combination of IMAС method and slow dilution at the presence of arginine, GSH/ GSSG and Mg2+ ions provided obtaining of rhIL7-BAPmut in pure and active form. Bifunctional activity of rhIL7-BAPmut after refolding is confirmed immunochemically by binding with specific antibodies. Conclusions. It was shown that application of rhIL7-BAPmut allows to reduce the time of the screening of immune combinatory libraries of variable genes of IgG and does not require specific primary and secondary antibodies. The rhIL7-BAPmut fusion protein also can be used for qualitative and quantitative analysis of IL-7 receptors.Keywords: IL-7, BAPmut, inclusion bodies, renaturation.","PeriodicalId":379244,"journal":{"name":"Visnik ukrains'kogo tovaristva genetikiv i selekcioneriv","volume":"79 5","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"113944412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The role of jasmonate signaling pathway in plant’s flowering genes response to ionizing radiation","authors":"M. Kryvokhyzha, K. Krutovsky, N. Rashydov","doi":"10.7124/visnyk.utgis.17.1.1200","DOIUrl":"https://doi.org/10.7124/visnyk.utgis.17.1.1200","url":null,"abstract":"Aim. This study aimed to characterize the role of the jasmonate signaling pathway in flowering genes response to acute and chronic ionizing irradiation in plants. Methods. We used the wild-type Arabidopsis thaliana and jasmonic pathway defective jin mutant of Col0 ecotype in our experiments. The chronic irradiation was provided by 137СsCl with a total dose of 17 cGy and a dose rate of 6.8×10-6 cGy/s. The acute irradiation experiment was performed on 21 days old plants at the 5.0 stage (Boyes 2001) by X-rays in a total dose of 5 Gy with a dose rate of 89 cGy/s. The length of stems and leaves was measured in post-irradiation period. The molecular genetic analysis was done using real-time PCR. We determined the relative expression of key flowering genes AP1, GI, FT, CO, ACT2 with UBQ10 used as reference genes. Statistical analysis of phenotypic parameters was done using Student’s t-test in GraphPad Prism 8 software. The quantitative PCR data were analyzed in the REST 2009 software, QIAGEN. Results. The plant groups differed significantly by the stem length (p>0,05). The study revealed decreased expression of CO, GI and FT genes in jin mutants. The overexpression of AP1 in jin mutants under chronic irradiation may cause cell division errors and impact flower development. In contrast, AP1 expression in WT plants was near to normal =1 under chronic irradiation. These results suggest the involvement of the jasmonate pathway in the regulation of plants flowering during the irradiation. Сonclusion. Based on the results of our study, we hypothesize that jasmonic acid has a stabilizing effect on the rate of cell differentiation in plants under chronic irradiation. Despite the uncovered role of jasmonic acid in Arabidopsis thaliana flowering the exact mechanism of its action remains unclear and requires further investigation.Keywords: jasmonate signaling, jasmonic acid, JA, flowering, ionizing radiation, real-time PCR, relative expression.","PeriodicalId":379244,"journal":{"name":"Visnik ukrains'kogo tovaristva genetikiv i selekcioneriv","volume":"53 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124741106","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dynamics of activity of energy supply enzymes of rat brain against the background of exposure to stress factors","authors":"A. Rashidova","doi":"10.7124/visnyk.utgis.17.1.1197","DOIUrl":"https://doi.org/10.7124/visnyk.utgis.17.1.1197","url":null,"abstract":"Aim. The aim of this work was to give a review of own studies of dynamics of the activity of energy metabolism enzymes in the brain during postnatal ontogenesis which characterizes the functional relations within the analysers, the destruction of their functions and the impact of adverse environmental factors on the body as well. Methods. The methods for the determination of the activity of energy metabolism enzymes in the brain of animals under the impact of adverse environmental factors and dysfunction of the analysers have been used. Results. It has been stated that the enzyme activity is regionally different in the tissues and sub cellular fractions of the brain depending on the animal species and the degree of the intensity of the stress factors, age and sex of the animal, circadian rhythms, season andmoreover, the resulting changes in the enzyme activity in most casesare irreversible. Conclusions. The data allow the dynamics of the activity of the energy supply enzymes to be considered as a determinant of cell reaction in response to exposure to adverse environmental factors and disturbance of sensory impulsation, which results in temporary increasing in the protection of intracellular energy metabolism through the development of an adaptive brain response.Key words: ontogenesis, brain, analysers, enzymes, environmental factors.","PeriodicalId":379244,"journal":{"name":"Visnik ukrains'kogo tovaristva genetikiv i selekcioneriv","volume":"50 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114333710","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evolution of darwinism. Synthetic theory of evolutions: 1926 – 1975 years","authors":"Y. V. Vagyn","doi":"10.7124/visnyk.utgis.17.1.1201","DOIUrl":"https://doi.org/10.7124/visnyk.utgis.17.1.1201","url":null,"abstract":"The process of combining Darwinism and genetics, which entered the history of biology as a synthetic theory of evolution, is considered.Key words: synthetic theory of evolution, neo-Darwinism, the concept of a biological species, population genetics, genetic polymorphism, the theory of dominance, gene drift.","PeriodicalId":379244,"journal":{"name":"Visnik ukrains'kogo tovaristva genetikiv i selekcioneriv","volume":"40 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123264247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Introduction to in vitro culture and callus initiation in Salvia hispanica L. (chia)","authors":"A. Z. Revutskaya, A. Holubenko, N. Nuzhyna, H. O. Rudik, N. Taran","doi":"10.7124/visnyk.utgis.17.1.1198","DOIUrl":"https://doi.org/10.7124/visnyk.utgis.17.1.1198","url":null,"abstract":"Aim. Preparation of aseptic seedlings Salvia hispanica L., callus initiation in vitro and establishment of primary explants suitable for the callus production. Methods. Seeds are sprouted on our own modification of conventional methods. The non-hormonal Murashige-Skoog agarized nutrient medium was used as basic medium for the experiments. Parts of one-month seedlings (roots, hypocotyl, cotyledon leaves) were used as explants for the use of the colza. We added growth regulators (BAP, 2,4-D) in different concentration combinations into the nutrient medium for callus initiation. Statistical processing was performed in Microsoft Office Excel. Results. Aseptic S. hispanica seedlings have been obtained. The callus growth was initiated on all types of explants, the dependence of the callus intensity on the type of explants and the growth regulators content in the nutrient medium was established. Morphogenic callus and root-regenerants have been obtained. Conclusions. Hypocotyl was the most suitable primary explant for callus growth. Seedlings, leaves and roots showed low morphogenetic capacity. The nutrient medium with an elevated 2,4-D content was the most effective for initiation of callus genesis and proliferation of non-morphogenous callus. A high concentration of 2,4-D in the medium improves S. hispanica callus growth but suppresses its morphogenic ability.Keywords: Salvia hispanica (Chia), in vitro culture, callus.","PeriodicalId":379244,"journal":{"name":"Visnik ukrains'kogo tovaristva genetikiv i selekcioneriv","volume":"30 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123155801","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Callus formation and organogenesis in tissue culture Deschampsia antarctica E. Desv.","authors":"I. Konvalyuk, L. P. Mozhylevs’ka, V. Kunakh","doi":"10.7124/visnyk.utgis.17.1.1196","DOIUrl":"https://doi.org/10.7124/visnyk.utgis.17.1.1196","url":null,"abstract":"Aim. The aim of the work was to determine the optimal conditions for induction and proliferation of tissue culture obtained from D. antarctica plants from various localities of the Maritime Antarctica. Methods. Tissue and organ culture techniques. Results. The media В5 supplemented with 2 mg/l 2,4-D + 0,1 mg/l BAP, В5 supplemented with 10 mg/l 2,4-D + 0,2 mg/l BAP and МС, supplemented with 5 mg/l 2,4-D + 0,1 mg/l Kin were optimal for callus induction from different types of explants. The media with a reduced concentrations of auxins and cytokinins were the most effective for maintenance of continuous tissue culture compared to the media for callus induction: B5 + 2 mg/l 2,4-D mg/l + 0,1 mg/l BAP and MC + 1 mg/l 2,4-D + 0.1 mg/l Kin. Tissues from shoot growth point and leaf explants of genotypes DAR12a and G/D12-2a on medium B5 with 2 mg/l 2,4-D + 0.1 mg/l BAP and B5 with 10 mg/l 2,4-D + 0,2 mg/l BAP demonstrated the ability to spontaneous organogenesis and formed separate shoots. Conclusions. Conditions have been determined for the induction and proliferation of tissue culture from leaf, root, and shoot growth point explants of D. antarctica. The frequency of callus formation depended on the mineral composition of medium, ratios and concentrations of growth regulators, type of explant, and genotype of a donor-plant. As a result of spontaneous organogenesis, regenerated plants were obtained, conditions for their rooting in vitro were elaborated. The proposed methods for induction and proliferation tissue culture of D. antarctica in vitro can be used to produce the plant material useful for a various investigations. Keywords: Deschampsia antarctica E. Desv., tissue culture, organogenesis in vitro, frequency of callogenesis.","PeriodicalId":379244,"journal":{"name":"Visnik ukrains'kogo tovaristva genetikiv i selekcioneriv","volume":"38 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-07-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115893804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}