Indonesian Journal of Life Sciences | ISSN: 2656-0682 (online)最新文献_第4页

Inulin from Several Tubers Available in Indonesia and the Growth of Gut Microbiota 印度尼西亚几种块茎中的菊粉与肠道菌群的生长

Indonesian Journal of Life Sciences | ISSN: 2656-0682 (online) Pub Date : 2020-03-31 DOI: 10.54250/IJLS.V2I1.33

I. Crespo, Augusto Arindra, Jessica Tashia Lualdi, Phoebe Anadita Rimba, Agus Budiawan Naro Putra, R. Rahardja

引用次数: 4

Prediction Methods of the Protein Subcellular Localization: A Systematic Reviews 蛋白质亚细胞定位的预测方法综述

Indonesian Journal of Life Sciences | ISSN: 2656-0682 (online) Pub Date : 2019-09-30 DOI: 10.54250/ijls.v1i2.20

A. A. Parikesit, Gabriella Patricia, Nanda Rizqia Pradana Ratnasari

引用次数: 0

Comparison of Antimicrobial Activity between Ethanolic Extract and Essential Oil of Ginger (Zingiber officinale) against Food-borne Bacteria 生姜醇提物与精油对食源性细菌抑菌活性的比较

Indonesian Journal of Life Sciences | ISSN: 2656-0682 (online) Pub Date : 2019-09-30 DOI: 10.54250/ijls.v1i2.21

Shania Foustine, Andre Andre, Bastian Setiadi, L. Hartiadi, Agnes Anania Triavika Sahamastuti

{"title":"Comparison of Antimicrobial Activity between Ethanolic Extract and Essential Oil of Ginger (Zingiber officinale) against Food-borne Bacteria","authors":"Shania Foustine, Andre Andre, Bastian Setiadi, L. Hartiadi, Agnes Anania Triavika Sahamastuti","doi":"10.54250/ijls.v1i2.21","DOIUrl":"https://doi.org/10.54250/ijls.v1i2.21","url":null,"abstract":"Background: Ginger (Zingiber officinale) is one of the most well-known spices with antimicrobial activity. However, different extraction methods of ginger will result in different antimicrobial properties due to the various substances extracted. This study aimed to compare antimicrobial activity between ethanolic extract and essential oil of ginger against food-borne bacteria grown in 2.1% Mueller Hinton agar. Methods: Fresh ginger rhizomes were extracted either by using vapor distillation method or maceration using ethanol to obtain ginger essential oils (GEO) and ginger ethanolic extract (GEE), respectively. Ethanolic extract and essential oil of ginger at different concentrations were then tested for their antimicrobial activity using disk diffusion method against Escherichia coli, Bacillus subtilis, Salmonella typhi, and Staphylococcus aureus. Tetracycline was also used as a standard of antibacterial agent. Results: Ginger essential oil in 25%, 50%, and 100% showed significant growth inhibition of four types of bacteria compared to ginger ethanolic extract. This antimicrobial effect of ginger essential oil was shown to be dose-dependent. However, it has been demonstrated that ginger ethanolic extract 50% and 100% has a stronger antimicrobial effect against B.subtilis. Conclusion: Ginger essential oil and ethanolic extract showed different degree of antibacterial activity against food-borne bacteria due to compound contained within respective extracts, with a higher degree of activity found in ginger essential oil. These may show that different ginger extract may have different antibacterial activity.","PeriodicalId":375737,"journal":{"name":"Indonesian Journal of Life Sciences | ISSN: 2656-0682 (online)","volume":"21 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122205367","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Combination of Piper betel Leaves and Areca catechu Nuts Ethanolic Extract Effects on In Vitro Antimicrobial Test against Aerobic Mouth Microbiota 槟榔叶与槟榔醇提物组合对需氧口腔微生物群体外抗菌试验的影响

Indonesian Journal of Life Sciences | ISSN: 2656-0682 (online) Pub Date : 2019-09-30 DOI: 10.54250/ijls.v1i2.24

Raymond Christanto, Andre Andre, Shania Foustine, L. Hartiadi

{"title":"Combination of Piper betel Leaves and Areca catechu Nuts Ethanolic Extract Effects on In Vitro Antimicrobial Test against Aerobic Mouth Microbiota","authors":"Raymond Christanto, Andre Andre, Shania Foustine, L. Hartiadi","doi":"10.54250/ijls.v1i2.24","DOIUrl":"https://doi.org/10.54250/ijls.v1i2.24","url":null,"abstract":"Introduction: Oral health awareness in Indonesia is still low with more and more oral and/or dental infection becomes more prevalent such as gingivitis, endodontitis, and pericoronitis. These infections could be caused by mouth microflora such as lactobacilli, Streptococcus mutans, Streptococcus mitis, Streptococcus oralis, and Streptococcus sobrinus. As time goes by, these bacteria could also become more problematic as antibacterial resistance emerges. Betel nut and betel leaf are well known traditional herbs that is often used in combination for \"nyirih\"; an Indonesian tradition to chew both plants often after meal. It is believed that these plants treat bad breath and also increases oral health as it could inhibit bacterial growth. Method: Betel nut and betel leaf were dried, powdered and macerated using 96% ethanol. The extract was tested on mouth microflora of volunteers grown in BHI using agar using disk diffusion test and modified E-test strip. Results: The betel leaves extract showed antibacterial activity in most of concentration used (1.25%, 2.5%, 5%, 10%) while betel nuts only gave antimicrobial effect in 10% concentration. The E-test results also showed the synergistic action between Piper betel leaves and Areca catechu nuts with some giving significant difference compared to the single treatment (P<0.05). Conclusion: Piper betel leaves gives antimicrobial effect with MIC value of 1.25% while Areca catechu have MIC value of 10%. Furthermore, combination of both resulted in synergistic effect as indicated with larger diameter of inhibition compared to the single treatments.","PeriodicalId":375737,"journal":{"name":"Indonesian Journal of Life Sciences | ISSN: 2656-0682 (online)","volume":"15 2","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"113935843","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Synergistic Antibacterial Activities of Ginger and Lemongrass Essential Oils as an Alternative Prevention to Food-Borne Disease 生姜和柠檬草精油对食源性疾病的协同抑菌作用

Indonesian Journal of Life Sciences | ISSN: 2656-0682 (online) Pub Date : 2019-09-30 DOI: 10.54250/ijls.v1i2.25

Agnes Anania Triavika Sahamastuti, Andre Andre, Shania Foustine, Anton Sumarpo, L. Hartiadi

{"title":"Synergistic Antibacterial Activities of Ginger and Lemongrass Essential Oils as an Alternative Prevention to Food-Borne Disease","authors":"Agnes Anania Triavika Sahamastuti, Andre Andre, Shania Foustine, Anton Sumarpo, L. Hartiadi","doi":"10.54250/ijls.v1i2.25","DOIUrl":"https://doi.org/10.54250/ijls.v1i2.25","url":null,"abstract":"Background: Lemongrass and ginger are traditional food ingredients in Asian countries, including Indonesia, Thailand, India, and Malaysia. Although their single essential oil has been assessed for its antibacterial activities, no report has been done for their combination. Material and methods: Our study evaluated single and combination of these herbs for their antibacterial properties against food-borne bacteria E. coli (NEB® catalog No. C2989K), B. subtilis (ATCC 6633), S. typhi (ATCC 14028) and S. aureus (InaCC B4). Essential oil of lemongrass and ginger were obtained by steam distillation and their antimicrobial were evaluated using disk diffusion assay with chloramphenicol as the standard antibiotic. Synergistic activity was assessed using the combination of materials at two or four-fold dilution from their respected MIC value. Results: We confirmed that single lemongrass and ginger essential oils inhibited the bacteria growth with MIC value of about 1-5% and 2.5-5%, respectively. Moreover, their synergism activities were observed when both were mixed at two-fold dilution from their respective MIC. Conclusion: We conclude that the combination of the ginger essential oils and lemongrass essential oils may have potential as a natural preservative to prevent food-borne diseases.","PeriodicalId":375737,"journal":{"name":"Indonesian Journal of Life Sciences | ISSN: 2656-0682 (online)","volume":"22 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114983560","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Toxicity of the Organophosphorus Pesticide Temephos 有机磷农药双硫磷的毒性研究

Indonesian Journal of Life Sciences | ISSN: 2656-0682 (online) Pub Date : 2019-09-30 DOI: 10.54250/ijls.v1i2.26

Dina Satriawan, Wibi Sindjaja, Timmy Richardo

{"title":"Toxicity of the Organophosphorus Pesticide Temephos","authors":"Dina Satriawan, Wibi Sindjaja, Timmy Richardo","doi":"10.54250/ijls.v1i2.26","DOIUrl":"https://doi.org/10.54250/ijls.v1i2.26","url":null,"abstract":"Dengue is a major public health problem in tropical urban areas, not only because it can quickly progress from the mild dengue fever to the deadly dengue hemorrhagic fever, but also because there is no single cure or licensed vaccine available to this day. To control the disease, the World Health Organization has recommended insecticides to control the number of mosquito vectors Aedes aegypti and Aedes albopictus. One of the main insecticides used is temephos, which inhibits the progression of the mosquito life cycle at the larvae stadium. Temephos is a member of the organophosphorus group of insecticides which is known to exhibit neurotoxicity through a common cholinergic pathway to insects and mammals. Despite its possible toxicity towards humans and other non-target organisms, temephos has been used widely to treat household water, including drinking-water and bath-water. Although clinical studies have yet shown any detrimental effects due to chronic consumption of temephos, studies on animal models have shown neurodevelopmental toxicity, while at the molecular level, exposure to tempehos has demonstrated genotoxic effects. Temephos is also considered an environmental contaminant and accumulation in soil and water have caused toxicity towards water organisms. Considering the extensive and repeated usage of temephos in public health, understanding and confirming the safety of temephos towards human health is crucial. Therefore, the objective of this paper is to review the current body of work available on the toxicity of temephos as a common dengue vector control.","PeriodicalId":375737,"journal":{"name":"Indonesian Journal of Life Sciences | ISSN: 2656-0682 (online)","volume":"102 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132424671","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Immunostimulatory effect of ikan peda (fermented Rastrelliger sp.) water extract on IgM production by HB4C5 cells 酵母水提物对HB4C5细胞产生IgM的免疫刺激作用

Indonesian Journal of Life Sciences | ISSN: 2656-0682 (online) Pub Date : 2019-01-25 DOI: 10.54250/ijls.v1i1.12

Michaela Maria Kanya Anindita, Agus Budiawan Naro Putra, T. Sugahara

{"title":"Immunostimulatory effect of ikan peda (fermented Rastrelliger sp.) water extract on IgM production by HB4C5 cells","authors":"Michaela Maria Kanya Anindita, Agus Budiawan Naro Putra, T. Sugahara","doi":"10.54250/ijls.v1i1.12","DOIUrl":"https://doi.org/10.54250/ijls.v1i1.12","url":null,"abstract":"Ikan peda is one of Indonesia’s traditional fermented food products. It uses mackerel fish (Rastrelliger sp.), also known as ikan kembung in Indonesia, as its main ingredient. It is made through fermentation by adding high concentration of salt in order to extend the shelf-life and bring out the desired flavor of the fish. Although fermented foods are known to be beneficial towards health, fewer studies have been reported regarding the actual health promoting potential of Indonesian fermented foods, especially ikan peda. Thus, the immunostimulatory effects of ikan peda extract were examined. Water-extract of ikan peda was tested to human-human hybridoma HB4C5 cells. It was found that ikan peda extract stimulates the production of IgM by HB4C5 cells according to ELISA results. Data from real-time RT-PCR result showed that ikan peda extract has the ability to increase IgM gene expression by 3-fold at the highest concentration treatment. Results from the heat and enzyme treatments showed that the active substance contained in the ikan peda extract was expected to be a heat-stable protein. Although, the active substance and the mechanism is yet to be determined, and can be considered for future experiments. Our findings suggest that consumption of ikan peda would contribute to the promotion of health.","PeriodicalId":375737,"journal":{"name":"Indonesian Journal of Life Sciences | ISSN: 2656-0682 (online)","volume":"62 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-01-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133273040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bioinformatics tools to Predict N-myristoylation Site a Comparison Study 生物信息学工具预测n -肉豆蔻酰化位点的比较研究

Indonesian Journal of Life Sciences | ISSN: 2656-0682 (online) Pub Date : 2019-01-17 DOI: 10.54250/ijls.v1i1.10

Muhammad Zainul Arifin, A. A. Parikesit

引用次数: 0

Computational Software for Assessing Allelic Droput 评估等位基因产量的计算软件

Indonesian Journal of Life Sciences | ISSN: 2656-0682 (online) Pub Date : 2019-01-17 DOI: 10.54250/ijls.v1i1.11

Jeremias Ivan, A. A. Parikesit

引用次数: 0