Protistology最新文献_第9页

Recombinant single chain antibodies as an instrument to search proteins involved in the interaction of microsporidia and other intracellular parasites with an infected host cell 重组单链抗体作为一种工具来搜索参与微孢子虫和其他细胞内寄生虫与受感染宿主细胞相互作用的蛋白质

Protistology Pub Date : 2019-01-01 DOI: 10.21685/1680-0826-2019-13-1-1

A. Tsarev, I. Senderskiy, S. A. Timofeev, V. Zhuravlyov, V. Dolgikh

{"title":"Recombinant single chain antibodies as an instrument to search proteins involved in the interaction of microsporidia and other intracellular parasites with an infected host cell","authors":"A. Tsarev, I. Senderskiy, S. A. Timofeev, V. Zhuravlyov, V. Dolgikh","doi":"10.21685/1680-0826-2019-13-1-1","DOIUrl":"https://doi.org/10.21685/1680-0826-2019-13-1-1","url":null,"abstract":"Obligate parasitism of microsporidia close to fungi protists causing widespread diseases of animals and immunodeficient patients, significantly complicates studies of their relationships with an infected host cell. Since microsporidia cannot be cultivated outside the host cell, genetic manipulations with them are extremely difficult. At the same time, long adaptation of microsporidia to the intracellular lifestyle, drastic minimization of their metabolic machinery, acquisition of unique transporters to exploit a host cell make these parasites a very valuable object for such study and require a search for new methods of investigation. Here, we describe our experiment on the construction of the library of recombinant single chain antibodies (scFv fragments) against proteins of fat bodies of locusts Locusta migratoria infected by the microsporidia Paranosema (Antonospora) locustae. The representativeness of this library was about 108 E. coli transformants carrying different combinations of variable fragments of heavy and light chains of immunized mice immunoglobulins. The first results of the selection of scFv fragments from the constructed library by phage display technology demonstrated that this approach may be effective to search proteins involved in the interaction of microsporidia and other intracellular parasites with an infected host cell. Cloning of selected genes into the expression vector, transformation of E. coli and screening two hundred bacterial colonies revealed scFv fragments against several such candidate proteins to begin their study. Further experiments with the library should discover new variants of recombinant antibodies interacting with the parasite and host proteins.","PeriodicalId":37502,"journal":{"name":"Protistology","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67885896","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Ruminal ciliate diversity of domestic cattle in Antalya, Turkey, with special emphasis on morphology of Entodinium rostratum (Entodiniomorphida, Ophryoscolecidae) 土耳其安塔利亚地区家牛瘤胃纤毛虫的多样性及其形态学特征

Protistology Pub Date : 2019-01-01 DOI: 10.21685/1680-0826-2019-13-2-5

G. Gürelli, Nuray Yürücüoğlu

引用次数: 0

Crithidia dobrovolskii sp. n. (Kinetoplastida: Trypanosomatidae) from parasitoid fly Lypha dubia (Diptera: Tachinidae): morphology and phylogenetic position 来自拟寄生蝇(双翅目:锥虫科)的dobrovolskii Crithidia sp. n.:形态和系统发育位置

Protistology Pub Date : 2019-01-01 DOI: 10.21685/1680-0826-2019-13-4-4

A. I. Ganyukova, M. N. Malysheva, P. A. Smirnov, A. Frolov

引用次数: 3

Professor Hans-Dieter Görtz (1945–2019). In memory of my close friend and colleague 汉斯-迪特教授Görtz(1945-2019)。为了纪念我的好朋友和同事

Protistology Pub Date : 2019-01-01 DOI: 10.21685/1680-0826-2019-13-2-6

S. Fokin

引用次数: 0

Cellular mechanisms of dinoflagellate cyst development and ecdysis – many questions to answer 鞭毛鞘囊肿发育和蜕皮的细胞机制-许多问题有待解答

Protistology Pub Date : 2019-01-01 DOI: 10.21685/1680-0826-2019-13-2-1

O. Matantseva

引用次数: 4

Diatom assemblages of the brackish Bolshaya Samoroda River (Russia) studied via light microscopy and DNA metabarcoding 利用光学显微镜和DNA元条形码技术研究了俄罗斯Bolshaya Samoroda河的半咸淡水硅藻组合

Protistology Pub Date : 2019-01-01 DOI: 10.21685/1680-0826-2019-13-4-5

Selivanov Ea, M. Ignatenko, T. Yatsenko-Stepanova, A. Plotnikov

{"title":"Diatom assemblages of the brackish Bolshaya Samoroda River (Russia) studied via light microscopy and DNA metabarcoding","authors":"Selivanov Ea, M. Ignatenko, T. Yatsenko-Stepanova, A. Plotnikov","doi":"10.21685/1680-0826-2019-13-4-5","DOIUrl":"https://doi.org/10.21685/1680-0826-2019-13-4-5","url":null,"abstract":"Diatoms are highly diverse and widely spread aquatic photosynthetic protists. Studies of regional patterns of diatom diversity are substantial for understanding taxonomy and biogeography of diatoms, as well as for ecological perspectives and applied purposes. DNA barcoding is a modern approach, which can resolve many problems of diatoms identification and can provide valuable information about their diversity in different ecosystems. However, only few studies focused on diatom assemblages of brackish rivers and none of them applied the genetic tools. Herein, we analyzed taxonomic composition and abundance of diatom assemblages in the brackish mixohaline Bolshaya Samoroda River flowing into the Elton Lake (Volgograd region, Russia) using light microscopy and high-throughput sequencing of the V4 region of the 18S rDNA gene amplicons. In total, light microscopy of the samples taken in 2011–2014 and 2018 allowed to distinguish 39 diatom genera, represented by 76 species and infraspecies taxa. Twenty three species of diatoms were recorded in the river for the first time. Next-generation sequencing revealed a larger number of diatom taxa (26 genera and 47 OTUs in two samples vs. 20 genera and 37 species estimated by light microscopy). As a result, sequences of Haslea, Fistulifera, Gedaniella were recorded in the river for the first time. Significant differences in the data obtained with molecular and light microscopy approaches are discussed. Some V4 18S rDNA sequences were characterized by a low similarity with homologues from the reference database. We revealed high spatial-temporal heterogeneity of the diatom assemblages, occurrence of freshwater species together with brackish and marine ones, and predominance of benthic and plankto-benthic species. Thus, investigations of diatoms in brackish rivers based on both morphological and molecular approaches provide a good chance of improving an understanding of diversity, ecology and biogeography of Bacillariophyta.","PeriodicalId":37502,"journal":{"name":"Protistology","volume":"47 7 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67888036","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Alexander Petrovich Mylnikov (1952–2019) 亚历山大·彼得罗维奇·米尔尼科夫（1952–2019）

Protistology Pub Date : 2019-01-01 DOI: 10.21685/1680-0826-2019-13-2-7

D. Tikhonenkov

引用次数: 0

A new species of Diderma from Bidoup Nui Ba National Park (southern Vietnam) 越南Bidoup Nui Ba国家公园Diderma属一新种

Protistology Pub Date : 2019-01-01 DOI: 10.21685/1680-0826-2019-13-3-2

Y. Novozhilov, I. Prikhodko, O. Shchepin

{"title":"A new species of Diderma from Bidoup Nui Ba National Park (southern Vietnam)","authors":"Y. Novozhilov, I. Prikhodko, O. Shchepin","doi":"10.21685/1680-0826-2019-13-3-2","DOIUrl":"https://doi.org/10.21685/1680-0826-2019-13-3-2","url":null,"abstract":"A new species of Diderma, described herein as D. dalatense, was found in ground leaf litter in mixed montane tropical forests (Bidoup Nui Ba National Park) of southern Vietnam. This species was discovered during intensive studies on the distribution and ecology of fungi and myxomycetes in tropical forests of Vietnam in the context of a long-term project Ecolan 1.2. The morphology of representative specimens was examined by light and scanning electron microscopy, and micrographs of relevant details are provided. D. dalatense has unique combination of morphological characters of the sporocarps among species of Diderma including very small (0.2–0.4 mm in diam.) dark-yellow, globose to subglobose sessile sporocarps, large conical or subglobose yellow columella, smooth peridium with three layers resembling that of Leocarpus and irregularly warted spores. In addition to the morphological description, partial sequences of three genetic markers of this new species (SSU, EF1A, COI) were obtained and submitted to GenBank. The stability of the taxonomic characters of the species was confirmed by several collections obtained in different localities of the National Park.","PeriodicalId":37502,"journal":{"name":"Protistology","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67886887","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 7

Mitochondrial genomes of Amoebozoa 变形虫的线粒体基因组

Protistology Pub Date : 2019-01-01 DOI: 10.21685/1680-0826-2019-13-4-1

N. Bondarenko, A. Smirnov, E. Nassonova, A. Glotova, A. Fiore-Donno

引用次数: 0

Real-time observations on the development of intranuclear parasite Nucleophaga amoebae (Rozellomycota) in the culture of Thecamoeba quadrilineata 四线变形虫培养过程中核内寄生阿米巴核食虫(Rozellomycota)发育的实时观察

Protistology Pub Date : 2019-01-01 DOI: 10.21685/1680-0826-2019-13-4-6

Olga Gordetskaya, Y. Mesentsev, O. Kamyshatskaya, R. Michel, J. Walochnik, A. Smirnov, E. Nassonova

引用次数: 3