Protistology最新文献_第7页

Phylogenetic analysis of the protein homologs of hemoxygenases HO-1 and HO-2 inferred from the transcriptomes of dinoflagellates 甲藻转录组中氧合酶HO-1和HO-2蛋白同源物的系统发育分析

Protistology Pub Date : 2021-01-01 DOI: 10.21685/1680-0826-2021-15-2-2

S. Pechkovskaya, N. Filatova

引用次数: 1

Comparative analysis of zoosporogenesis’ genes of the bastoclad Blastocladiella emersonii and the aphelid Paraphelidium tribonematis reveals the new directions of evolutionary research 对生孢子虫和非生孢子虫的动物孢子发生基因的比较分析揭示了进化研究的新方向

Protistology Pub Date : 2021-01-01 DOI: 10.21685/1680-0826-2021-15-1-2

I. Pozdnyakov, A. Zolotarev, S. Karpov

{"title":"Comparative analysis of zoosporogenesis’ genes of the bastoclad Blastocladiella emersonii and the aphelid Paraphelidium tribonematis reveals the new directions of evolutionary research","authors":"I. Pozdnyakov, A. Zolotarev, S. Karpov","doi":"10.21685/1680-0826-2021-15-1-2","DOIUrl":"https://doi.org/10.21685/1680-0826-2021-15-1-2","url":null,"abstract":"The aphelids, intracellular parasitoids of algae, have a life cycle similar to zoosporic fungi Chytridiomycota and Blastocladiomycota, and are positioned as a sister clade to all fungi on the recent multigene phylogenetic tree. The fungi and aphelids might possibly have a common ancestor with a complex life cycle somewhat similar to modern zoosporic fungi. To investigate this possibility we have analyzed the genes that increase expression during zoosporogenesis of the blastoclad fungus Blastocladiella emersonii based on the transcriptomic data of Vieira and Gomes (2013), and described the course of sporogenesis at the molecular level. Homologs of genes from the B. emersonii gene set were found in various lineages of the Opisthokonta group, and specifically in the transcriptome of Paraphelidium tribonematis . We calculated the percentage ratios of genes that formed common functional groups and the genes with homologs in various clades of related organisms. We found that zoospore production of a blastoclad fungus is a multi-phase process, where switching of the regulatory elements takes place. The analyzed genes are distributed as follows: 81% are common for all Opisthokonta, 16% are specific for Fungi and only 3% are common to Fungi and aphelids but none are found in the Holozoa lineage. Based on these data we propose a hypothesis on the independent origin of the life cycle in Fungi and Aphelida from a polymorphic ancestor.","PeriodicalId":37502,"journal":{"name":"Protistology","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67888911","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

In shadow of Holospora – The continuous quest for new Holosporaceae members 在全息孢子的阴影下-不断寻找新的全息孢子科成员

Protistology Pub Date : 2021-01-01 DOI: 10.21685/1680-0826-2021-15-3-3

A. Potekhin, I. Nekrasova, Felicitas E. Flemming

引用次数: 3

Brefeldin A disturbs Golgi complex integrity but does not impair ecdysis in the dinoflagellates Prorocentrum cordatum Brefeldin A干扰高尔基复合体的完整性，但不损害双鞭毛藻心状原的代谢

Protistology Pub Date : 2021-01-01 DOI: 10.21685/1680-0826-2021-15-2-3

I. Pozdnyakov, M. Berdieva, V. Kalinina, S. Pechkovskaya, O. Matantseva

{"title":"Brefeldin A disturbs Golgi complex integrity but does not impair ecdysis in the dinoflagellates Prorocentrum cordatum","authors":"I. Pozdnyakov, M. Berdieva, V. Kalinina, S. Pechkovskaya, O. Matantseva","doi":"10.21685/1680-0826-2021-15-2-3","DOIUrl":"https://doi.org/10.21685/1680-0826-2021-15-2-3","url":null,"abstract":"Summary The marine planktonic dinoflagellate Prorocentrum cordatum (syn. Prorocentrum minimum ) is a thecate species capable of ecdysis, i.e. rearrangement of the cell covering in response to different stressors. During the process of ecdysis, P. cordatum cells shed the plasma membrane and outer amphiesmal vesicle membrane, and become immotile, covered only by thecal plates and the new plasma membrane. At this stage, the cells can be considered as thecal cysts. Then, they complete ecdysis by leaving the old thecal plates and restoring motility, i.e. excyst. To elucidate the role of vesicular transport in the cell covering rearrangement, we tested the effect of brefeldin A, the inhibitor of vesicular transport from the endoplasmic reticulum to the Golgi complex widely used in cell biology, on the cellular structure of P. cordatum and its ability to ecdyse. We found out that brefeldin A blocked vesicular trafficking from the endoplasmic reticulum to the Golgi complex, since we observed disassembly of the Golgi complex in cells treated by brefeldin A at 0.1 µg/ml for 1 h. Moreover, such a treatment did not increase mortality in P. cordatum culture at the time scale of 6 h. Nevertheless, our experiments demonstrated that brefeldin A affected neither shedding of outer membranes nor discarding of thecal plates during the stressor-induced ecdysis. Based on our findings, we conclude that vesicle trafficking from the endoplasmic reticulum to the Golgi complex and further to the plasma membrane is not essential to the process of membrane and theca shedding. However, this route still can be involved in the process of new amphiesma’ maturation, which is discussed in this work.","PeriodicalId":37502,"journal":{"name":"Protistology","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67889594","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A possible molecular mechanism of Chlamydomonas reinhardtii adaptation to different trophic conditions 莱茵衣藻适应不同营养条件的可能分子机制

Protistology Pub Date : 2021-01-01 DOI: 10.21685/1680-0826-2021-15-3-7

R. Puzanskiy, D. Romanyuk, A. Shavarda, V. Yemelyanov, M. Shishova

引用次数: 2

Boris Vasilievich Gromov (1933–2001): a microbiologist for all seasons 鲍里斯·瓦西里耶维奇·格罗莫夫(1933-2001):四季的微生物学家

Protistology Pub Date : 2021-01-01 DOI: 10.21685/1680-0826-2021-15-3-1

A. Pinevich

引用次数: 0

The impact of nuisance planktonic invaders on pelagic communities: a review of the Baltic Sea case studies 有害浮游生物入侵对远洋生物群落的影响:波罗的海案例研究综述

Protistology Pub Date : 2021-01-01 DOI: 10.21685/1680-0826-2021-15-4-2

I. Telesh, E. Naumenko