Nematological Research (Japanese Journal of Nematology)最新文献_第3页

Limited distribution of Caenorhabditis japonica in Japan 日本隐杆线虫在日本的有限分布

Nematological Research (Japanese Journal of Nematology) Pub Date : 2018-12-20 DOI: 10.3725/jjn.48.71

T. Yoshiga

引用次数: 0

The composition of southern root-knot nematode (Meloidogyne incognita) race in the resistance test field used for sweetpotato breeding 甘薯抗性试验田南方根结线虫(Meloidogyne incognita)小种的组成

Nematological Research (Japanese Journal of Nematology) Pub Date : 2018-07-25 DOI: 10.3725/jjn.48.31

T. Kuranouchi, Y. Momota, Takada Akiko, K. Katayama

引用次数: 1

Application of a simple and high-throughput DNA extraction method to real-time PCR quantification of target plant-parasitic nematodes in nematode communities 一种简单、高通量的DNA提取方法在线虫群落中目标植物-寄生线虫实时PCR定量中的应用

Nematological Research (Japanese Journal of Nematology) Pub Date : 2018-07-25 DOI: 10.3725/JJN.48.1

Masanori Kawanobe, K. Toyota

{"title":"Application of a simple and high-throughput DNA extraction method to real-time PCR quantification of target plant-parasitic nematodes in nematode communities","authors":"Masanori Kawanobe, K. Toyota","doi":"10.3725/JJN.48.1","DOIUrl":"https://doi.org/10.3725/JJN.48.1","url":null,"abstract":"Molecular techniques are prevailing in nematode identification and quantification, for which DNA extraction from nematodes is essential. However, the published DNA extraction methods are laborious and often require various expensive consumables and high-end equipment. In order to prepare DNA templates for a high-throughput real-time PCR assay, the present study modified a conventional DNA extraction method of Naklha et al. (2010) from nematode suspensions with ordinary lab equipment and achieved results and advantages comparable with two other conventional methods. The results of real-time PCR assays for quantifying Pratylenchus zeae, Tylenchorhynchus leviterminalis and Hoplolaimus sp. using the new protocol were highly correlated with those obtained by morphological counts and comparable to or more sensitive than those obtained by two conventional methods. Although the new protocol took over 100 min for DNA extraction, the manual processing took less than 10 min, i.e., half to one-fourth of the other methods. The running cost was less than half to one-tenth of the other methods. Nematol. Res. 48(1), 1–10 (2018).","PeriodicalId":369433,"journal":{"name":"Nematological Research (Japanese Journal of Nematology)","volume":"11 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127910901","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The composition of hindgut microbiota of Periplaneta japonica in the presence of thelastomatid parasitic nematodes 寄生线虫存在下日本大蠊后肠菌群的组成

Nematological Research (Japanese Journal of Nematology) Pub Date : 2018-07-25 DOI: 10.3725/JJN.48.19

Cláudia S. L. Vicente, Sota Ozawa, Koichi Hasegawa

{"title":"The composition of hindgut microbiota of Periplaneta japonica in the presence of thelastomatid parasitic nematodes","authors":"Cláudia S. L. Vicente, Sota Ozawa, Koichi Hasegawa","doi":"10.3725/JJN.48.19","DOIUrl":"https://doi.org/10.3725/JJN.48.19","url":null,"abstract":"Thelastomatid nematodes (Nematoda: Oxyurida: Thelastomatoidea) are obligatory parasites that occur naturally in the hindgut of arthropods. Their origin and impact in the host is still unknown. Previous studies showed that the presence of thelastomatid nematodes in the gut of cockroaches ( Periplaneta fuliginosa and P. americana ) could influence the composition of their hindgut microflora. Through a metagenomic approach ( 16 S rRNA V 3 -V 4 sequencing), we have characterized the hindgut microbiome of P. japonica in the presence of thelastomatid nematodes (L 1986 , natural parasitic nematode Protrellus sp. present as a natural infection condition; and L 1987 , non-native parasitic nematode Leidynema appendiculatum present as an artificial infection condition). The hindgut microbiome of P. japonica in both conditions were mainly composed of Bacteroidetes, Firmicutes and Proteobacteria. Moreover, the natural and artificial infection by thelastomatid nematodes lead to shifts in the relative abundance of these main resident flora as seen in P. americana . The OTUs percentage of Bacteroidetes and Proteobacteria were higher in P. japonica infected with Protrellus sp. (L 1986 ) than in P. japonica infected by L. appendiculatum (L 1987 ), while OTUs from Firmicutes phylum was higher in L 1987 than in L 1986 . This study fosters a detailed investigation in the role played by these animal parasites in their host insect.","PeriodicalId":369433,"journal":{"name":"Nematological Research (Japanese Journal of Nematology)","volume":"56 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123821680","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Reproduction of tobacco cyst nematode Globodera tabacum on several Japanese tomato cultivars 烟草囊线虫在几个日本番茄品种上的繁殖

Nematological Research (Japanese Journal of Nematology) Pub Date : 2018-07-25 DOI: 10.3725/JJN.48.27

T. Uehara, Y. Momota

引用次数: 2

Case study on a modified method to quantify the density of some soil-borne plant-parasitic nematodes in a simpler and less expensive way 一种改良方法的案例研究，以更简单和更便宜的方式量化一些土壤传播的植物寄生线虫的密度

Nematological Research (Japanese Journal of Nematology) Pub Date : 2018-07-25 DOI: 10.3725/JJN.48.11

Zejun Cheng, Sayo Shirai, K. Toyota, K. Ritz

{"title":"Case study on a modified method to quantify the density of some soil-borne plant-parasitic nematodes in a simpler and less expensive way","authors":"Zejun Cheng, Sayo Shirai, K. Toyota, K. Ritz","doi":"10.3725/JJN.48.11","DOIUrl":"https://doi.org/10.3725/JJN.48.11","url":null,"abstract":"Quantification of plant-parasitic nematodes (PPN) in soil with real-time PCR is a useful diagnosis to estimate damage to crops. However, previously reported methods involve high consumable and labor costs. The objectives of this study were to combine previously reported methods for soil pretreatment, DNA extraction and real-time PCR to quantify the density of soil-borne PPN in a simpler and less expensive way and to confirm the usefulness of a new simple method. Soils infested with either Heterodera glycines (soybean cyst nematode), Ditylenchus destructor (potato rot nematode) or Meloidogyne incognita (root-knot nematode) were ball-milled. DNA was then extracted with phosphate buffer and purified with a commercially available column. Real-time PCR was conducted to quantify the target nematodes. The cycle threshold (Ct) values obtained by the new method showed highly significant correlations with those by the conventional method for all three species (R2 > 0.75). Significant correlations (R2 > 0.987) were also obtained between the Ct values and the numbers of nematodes inoculated into soils. The DNA extraction from 6 samples by the new simple method required only 1 hr and about $4.8 of consumables, while that by the conventional method required 3 hr and about $12 of consumables. These results demonstrate that the method consisting of ballmilling and simple DNA extraction enables rapid and less expensive quantification of nematodes in soils. Nematol. Res. 48(1), 11–17 (2018).","PeriodicalId":369433,"journal":{"name":"Nematological Research (Japanese Journal of Nematology)","volume":"7 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125348485","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Suppressive Bacteria Associated with Plant Parasitic Nematodes in Egyptian Agriculture 埃及农业中与植物寄生线虫相关的抑制细菌

Nematological Research (Japanese Journal of Nematology) Pub Date : 1994-12-22 DOI: 10.3725/JJN1993.24.2_55

M. El-Sherif, A. Ali, M. Barakat

引用次数: 26