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Nematological Research (Japanese Journal of Nematology)最新文献

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The insecticidal activity of an entomopathogenic nematode Heterorhabditis bacteriophora and its symbiotic bacterium Photorhabdus luminescens against Periplaneta and Blattella cockroaches 昆虫病原线虫嗜菌异habditis bacteriophora及其共生细菌Photorhabdus luminesens对大蠊和小蠊的杀虫活性
Nematological Research (Japanese Journal of Nematology) Pub Date : 2020-12-20 DOI: 10.3725/JJN.50.27
Takahiro Hamaguchi, Daiki Sawanomukai, Kazuki Sato, Sota Ozawa, D. Kalaiselvi, Koichi Hasegawa
{"title":"The insecticidal activity of an entomopathogenic nematode Heterorhabditis bacteriophora and its symbiotic bacterium Photorhabdus luminescens against Periplaneta and Blattella cockroaches","authors":"Takahiro Hamaguchi, Daiki Sawanomukai, Kazuki Sato, Sota Ozawa, D. Kalaiselvi, Koichi Hasegawa","doi":"10.3725/JJN.50.27","DOIUrl":"https://doi.org/10.3725/JJN.50.27","url":null,"abstract":"japonica, however, did not differ from that of the control at all of the tested concentrations; whereas, the mortality of B. germanica was relatively higher than that of Periplaneta cockroaches. Second, we tested the pathogenicity of P. luminescens alone against the three Periplaneta species by injection of bacterial cells. All tested cockroaches were found to be killed within 2 – 4 days. These results would suggest that Periplaneta are susceptible to P. luminescens ; however, in the bait experiment , H. bacteriophora was unable to effectively establish a lethal infection of P. luminescens . Our results indicate that susceptibility to EPN infection and bacterial pathogenicity should be evaluated separately, and that much less H. bacteriophora is required to kill B. germanica than is needed to kill three Periplaneta cockroaches. Nematol. Res. 50 ( 2 ), 27 – 33 . ( 2020 )","PeriodicalId":369433,"journal":{"name":"Nematological Research (Japanese Journal of Nematology)","volume":"83 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132403443","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Does solanoeclepin A act as a host-searching cue for Globodera rostochiensis? solanoeclepin A是否作为罗斯托奇Globodera的宿主搜索线索?
Nematological Research (Japanese Journal of Nematology) Pub Date : 2020-09-20 DOI: 10.3725/JJN.50.9
Itaru Sakata, A. Kushida, T. Narabu, K. Tanino
{"title":"Does solanoeclepin A act as a host-searching cue for Globodera rostochiensis?","authors":"Itaru Sakata, A. Kushida, T. Narabu, K. Tanino","doi":"10.3725/JJN.50.9","DOIUrl":"https://doi.org/10.3725/JJN.50.9","url":null,"abstract":"","PeriodicalId":369433,"journal":{"name":"Nematological Research (Japanese Journal of Nematology)","volume":"28 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116612521","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Development of primers for PCR diagnosis of Xiphinema species associated with Japanese traditional ornamental trees 日本传统观赏树刺蝇属植物PCR诊断引物的开发
Nematological Research (Japanese Journal of Nematology) Pub Date : 2020-09-20 DOI: 10.3725/JJN.50.13
Y. Tateishi, T. Uehara
{"title":"Development of primers for PCR diagnosis of Xiphinema species associated with Japanese traditional ornamental trees","authors":"Y. Tateishi, T. Uehara","doi":"10.3725/JJN.50.13","DOIUrl":"https://doi.org/10.3725/JJN.50.13","url":null,"abstract":"","PeriodicalId":369433,"journal":{"name":"Nematological Research (Japanese Journal of Nematology)","volume":"148 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128440162","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Mermithid nematodes isolated from the shield bug Parastrachia japonensis 从日本盾蝽中分离的Mermithid线虫
Nematological Research (Japanese Journal of Nematology) Pub Date : 2020-09-20 DOI: 10.3725/JJN.50.1
Tomomi Iryu, Ryusei Tanaka, T. Yoshiga
{"title":"Mermithid nematodes isolated from the shield bug Parastrachia japonensis","authors":"Tomomi Iryu, Ryusei Tanaka, T. Yoshiga","doi":"10.3725/JJN.50.1","DOIUrl":"https://doi.org/10.3725/JJN.50.1","url":null,"abstract":"","PeriodicalId":369433,"journal":{"name":"Nematological Research (Japanese Journal of Nematology)","volume":"17 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2020-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125671483","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Identification of the potato cyst nematodes based on two-step multiplex endpoint PCR with the dUTP/UNG system for carry-over prevention 基于dUTP/UNG预防结转的两步多重端点PCR鉴定马铃薯囊肿线虫
Nematological Research (Japanese Journal of Nematology) Pub Date : 2019-12-20 DOI: 10.3725/jjn.49.19
Hiromichi Sakai, A. Kushida, T. Narabu
{"title":"Identification of the potato cyst nematodes based on two-step multiplex endpoint PCR with the dUTP/UNG system for carry-over prevention","authors":"Hiromichi Sakai, A. Kushida, T. Narabu","doi":"10.3725/jjn.49.19","DOIUrl":"https://doi.org/10.3725/jjn.49.19","url":null,"abstract":"Multiplex endpoint PCR techniques are among the essential diagnostic tools used for identifying the potato cyst nematodes (PCNs: Globodera rostochiensis and G. pallida ). Multiplex endpoint PCR assays for PCNs published to date, based on three-step PCR, are not integrated with primers to verify successful PCR in testing non-target species. Besides, carry-over contamination is a serious problem in diagnostic PCR assays. To improve the run time and reliability of the endpoint PCR test, we developed a two-step multiplex PCR identification method for PCNs using the dUTP/UNG carry-over prevention system. For this purpose, primers with high melting temperatures were newly designed to amplify mitochondrial DNA fragments specific to the respective PCN species and the nuclear ribosomal RNA gene fragments as PCR positive controls across cyst nematodes. In addition, the DNA preparation method from juveniles and cysts was simplified using sodium dodecyl sulfate and disposable homogenizers. This multiplex amplification generated amplicons of 150 bp, 287 bp and ca . 450 bp for G. rostochiensis , G. pallida and the non-target cyst nematode species, respectively. No cross-reactions were observed among the tested nematodes including 11 species and 22 populations. In examining mixed juveniles and cysts of PCNs, our method successfully detected both species even in the ratio of one to ten. These PCR runs took ca . 1 h, faster than the other three-step PCR protocols. The new PCR diagnostic method described here is reliable, fast and thus a good alternative to the other assays based on conventional PCR for diagnosis of PCNs. Nematol. Res. 49 ( 2 ), 19 – 27 . ( 2019 )","PeriodicalId":369433,"journal":{"name":"Nematological Research (Japanese Journal of Nematology)","volume":"6 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122624834","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 5
Insect parasitic nematodes, Contortylenchus sp. (Tylenchida: Allantonematidae) from the spruce bark beetle Ips typographus and the large larch bark beetle I. cembrae (Coleoptera: Scolytidae) in Japan 日本云杉树皮甲虫Ips typographus和大落叶松树皮甲虫I. cembrae(鞘翅目:柱头虫科)的昆虫寄生线虫(柱头虫目:Allantonematidae
Nematological Research (Japanese Journal of Nematology) Pub Date : 2019-12-20 DOI: 10.3725/jjn.49.45
H. Kosaka, N. Kanzaki
{"title":"Insect parasitic nematodes, Contortylenchus sp. (Tylenchida: Allantonematidae) from the spruce bark beetle Ips typographus and the large larch bark beetle I. cembrae (Coleoptera: Scolytidae) in Japan","authors":"H. Kosaka, N. Kanzaki","doi":"10.3725/jjn.49.45","DOIUrl":"https://doi.org/10.3725/jjn.49.45","url":null,"abstract":"Parasitic nematodes were found in the two bark beetle species Ips typographus and I. cembrae in Japan. Based on their ecological and morphological characteristics, these nematodes are identified as members of the genus Contortylenchus . They show some differences in not only parasitic manners but also molecular profiles. Ribosomal DNA sequences for small subunit (SSU), and D 2 -D 3 expansion segments of the large subunit (D 2 -D 3 LSU) of the nematodes in the two different beetle species are almost identical, but they show one base-pair substitution in the D 2 -D 3 LSU. These results suggest that the nematodes from I. typographus and I. cembrae are closely related but separate species. Interestingly, the D 2 -D 3 LSU of the nematode in I. typographus was identical to those of both Contortylenchus sp. from the Moscow region, the Russian Federation, and C. diplogaster in I. cembrae from the Czech Republic. This result indicates that the nematode in I. typographus is closely related to or could be conspecific to the European Contortylenchus species, including especially C. diplogaster or a related species, C. pseudodiplogaster . Nematol. Res. 49 ( 2 ), 45 – 47 . ( 2019 )","PeriodicalId":369433,"journal":{"name":"Nematological Research (Japanese Journal of Nematology)","volume":"155 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122159409","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Sugarcane yield loss in the ratoon crop carried over from the plant crop damaged by plant-parasitic nematode in a heavy clay field in Okinawa, Japan 在日本冲绳的一块重粘土田，甘蔗产量损失是由植物寄生线虫对植物作物造成的
Nematological Research (Japanese Journal of Nematology) Pub Date : 2019-09-20 DOI: 10.3725/jjn.49.1
Masanori Kawanobe, N. Miyamaru, Koichi Yoshida, Takeshi Kawanaka, Tomonori Fujita, K. Toyota
{"title":"Sugarcane yield loss in the ratoon crop carried over from the plant crop damaged by plant-parasitic nematode in a heavy clay field in Okinawa, Japan","authors":"Masanori Kawanobe, N. Miyamaru, Koichi Yoshida, Takeshi Kawanaka, Tomonori Fujita, K. Toyota","doi":"10.3725/jjn.49.1","DOIUrl":"https://doi.org/10.3725/jjn.49.1","url":null,"abstract":"","PeriodicalId":369433,"journal":{"name":"Nematological Research (Japanese Journal of Nematology)","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130226740","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 5
First record of Cyathostoma (Hovorkonema) sp. (Nematoda: Syngamidae) from a red-crowned crane, Grus japonensis, in Kushiro, Hokkaido, Japan 日本北海道Kushiro地区首次记录日本丹顶鹤Cyathostoma (Hovorkonema) sp.(线虫纲:Syngamidae)
Nematological Research (Japanese Journal of Nematology) Pub Date : 2019-09-20 DOI: 10.3725/jjn.49.7
T. Yoshino, Hiroko Iima, Fumio Matsumoto, M. Asakawa
{"title":"First record of Cyathostoma (Hovorkonema) sp. (Nematoda: Syngamidae) from a red-crowned crane, Grus japonensis, in Kushiro, Hokkaido, Japan","authors":"T. Yoshino, Hiroko Iima, Fumio Matsumoto, M. Asakawa","doi":"10.3725/jjn.49.7","DOIUrl":"https://doi.org/10.3725/jjn.49.7","url":null,"abstract":"","PeriodicalId":369433,"journal":{"name":"Nematological Research (Japanese Journal of Nematology)","volume":"24 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123861255","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Occurrence of Meloidogyne microcephala in Oita Prefecture 大分县小头畸形发生情况
Nematological Research (Japanese Journal of Nematology) Pub Date : 2019-09-20 DOI: 10.3725/jjn.49.13
Tomonori Suzuki, H. Yamano, H. Iwahori
{"title":"Occurrence of Meloidogyne microcephala in Oita Prefecture","authors":"Tomonori Suzuki, H. Yamano, H. Iwahori","doi":"10.3725/jjn.49.13","DOIUrl":"https://doi.org/10.3725/jjn.49.13","url":null,"abstract":"","PeriodicalId":369433,"journal":{"name":"Nematological Research (Japanese Journal of Nematology)","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129491519","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Population densities of the pine wood nematode, Bursaphelenchus xylophilus, in dead pine trees caused by pine wilt disease in cool areas of Japan 日本寒区松树枯萎病引起的枯死松树中松材线虫种群密度
Nematological Research (Japanese Journal of Nematology) Pub Date : 2018-12-20 DOI: 10.3725/jjn.48.63
Y. Mamiya, Kazumi Kobayashi, Kazuhiko Hoshizaki, Asami Yoshida, Kazumasa Ohta
{"title":"Population densities of the pine wood nematode, Bursaphelenchus xylophilus, in dead pine trees caused by pine wilt disease in cool areas of Japan","authors":"Y. Mamiya, Kazumi Kobayashi, Kazuhiko Hoshizaki, Asami Yoshida, Kazumasa Ohta","doi":"10.3725/jjn.48.63","DOIUrl":"https://doi.org/10.3725/jjn.48.63","url":null,"abstract":"Pine wilt disease caused by the pine wood nematode, Bursaphelenchus xylophilus , (PWN), is the most devastating disease of pine trees ( Pinus densiflora and P. thunbergii ) in Japan. The disease has spread to the northern part of Japan, where the cool climate (annual mean temperature < 12℃ ) delays disease development. The current study, which was conducted in northern Japan, demonstrated PWN population dynamics in dead pine trees infected with PWN in the previous year and died in early spring through early summer. PWN numbers in dead trees were lower in the cold climate than previously reported in warm climates. PWNs were widely distributed throughout each dead tree and had a contagious distribution. Oviposition scars of the PWN vector, Monochamus alternatus , were not detected on the dead trees killed in spring to early summer because the time of disease development and tree death did not coincide with the time of insect oviposition. Nematol. Res. 48 ( 2 ), 63 – 70 ( 2018 ).","PeriodicalId":369433,"journal":{"name":"Nematological Research (Japanese Journal of Nematology)","volume":"12 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129341168","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
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