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First report of anthracnose on Fagus sylvatica caused by Colletotrichum fioriniae in Italy 意大利森林Fagus sylvatica炭疽病首次报道

New Disease Reports Pub Date : 2023-10-01 DOI: 10.1002/ndr2.12226

I. Giubilei, F. Brugneti, S. Turco, M. I. Drais, A. Mazzaglia

{"title":"First report of anthracnose on Fagus sylvatica caused by Colletotrichum fioriniae in Italy","authors":"I. Giubilei, F. Brugneti, S. Turco, M. I. Drais, A. Mazzaglia","doi":"10.1002/ndr2.12226","DOIUrl":"https://doi.org/10.1002/ndr2.12226","url":null,"abstract":"European beech (Fagus sylvatica) is one of the most common deciduous forest species in Italy and across Europe, important for its economic, cultural and environmental value (Siddique et al., 2021). In June 2023, leaves showing anthracnose symptoms (Figure 1) were collected from the UNESCO site “Faggeta Vetusta di Monte Raschio” located in Oriolo Romano, Viterbo in central Italy. To identify the causal agent, leaves were surface sterilised by dipping successively in 70% ethanol for two minutes, 1% sodium hypochlorite for three minutes and 70% ethanol for one minute, and finally rinsed three times with sterile water (SDW). Slices obtained with a sterile scalpel were plated on potato dextrose agar plates and incubated at 25°C for 8 to 15 days. Developing fungal colonies had white to grey cottony mycelium and were pale orange with dark flecking from the reverse side (Figure 2). Conidia were hyaline, smooth-walled, aseptate, narrowly elliptical pointed at both ends, measuring 10.5-21 μm (mean 16.4 μm) × 3.5-7 μm (mean 4.3 μm) (Figure 3). The morphological characteristics fit published descriptions of Colletotrichum fioriniae (Shivas & Yu, 2009; Damm et al., 2012). Genomic DNA of three representative isolates (FAG14, FAG15 and FAG16) were extracted from 100 mg of fresh mycelium using the NucleoSpin®PlantII kit manufacturer's protocol for fungi (Macherey-Nagel,Germany). The ribosomal internal transcribed spacer (ITS), β-tubulin (TUB2), actin (ACT), partial chitin synthase (CHS-1), histone 3 (HIS3) and a 200-bp intron of the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) regions were amplified and sequenced. A BLASTn search of the sequences (GenBank Accession Nos. OR587916-18 for ITS, OR596663-65 for TUB2, OR596666-68 for ACT, OR596669-71 for CHS-1, OR596672-74 for HIS3, OR596675:77 for GAPDH) revealed 100% identity with C. fioriniae. Pathogenicity tests were done using pure cultures of the FAG14 isolate. Healthy beech leaves were surface sterilised and inoculated with 10 μL of a spore suspension (106 conidia/mL) on the surface. Leaves inoculated with SDW were used as a control. The inoculated samples were placed in closed sterile boxes and kept at 25°C and 100% relative humidity. Seven to ten days after inoculation, the leaves had necrotic spots, whereas the controls remained healthy (Figure 4). To fulfil Koch's postulates, C. fioriniae was reisolated from the symptomatic leaves and its identity was confirmed both morphologically and molecularly. To the best of our knowledge, this pathogen has only previously been reported on Fagus sylvatica in seedlings in a nursery in Poland (Pszczółkowska et al., 2017). Thus, this is the first report of C. fioriniae causing anthracnose on adult trees of Fagus sylvatica in a natural forest in Italy. This study is part of the Agritech National Research Center and funded by the European Union Next-Generation EU.","PeriodicalId":36931,"journal":{"name":"New Disease Reports","volume":"40 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136167596","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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First report of Colletotrichum coccodes causing black dot on potato in Mauritius 毛里求斯马铃薯炭疽病菌黑点首次报道

New Disease Reports Pub Date : 2023-10-01 DOI: 10.1002/ndr2.12224

S. D. Takooree, H. Neetoo, V. M. Ranghoo‐Sanmukhiya

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First report of a naturally occurring isolate of Pepper yellow vein Mali virus causing tobacco yellow leaf curl disease in Burkina Faso 在布基纳法索首次报道一种引起烟草黄叶卷曲病的自然产生的辣椒黄脉马里病毒分离物

New Disease Reports Pub Date : 2023-10-01 DOI: 10.1002/ndr2.12217

A. Ouattara, T. A. Nana, K. H. Sogoba, K. Koïta, P. Lefeuvre, J. M. Lett

{"title":"First report of a naturally occurring isolate of Pepper yellow vein Mali virus causing tobacco yellow leaf curl disease in Burkina Faso","authors":"A. Ouattara, T. A. Nana, K. H. Sogoba, K. Koïta, P. Lefeuvre, J. M. Lett","doi":"10.1002/ndr2.12217","DOIUrl":"https://doi.org/10.1002/ndr2.12217","url":null,"abstract":"Pepper yellow vein disease (PYVD) has been reported to be associated with the African monopartite begomovirus Pepper yellow vein Mali virus (PepYVMLV) in West Africa (Tiendrébéogo et al., 2008; Zhou et al., 2008). Recently, vegetable isolates of PepYVMLV have also been shown to be associated with a DNA-B component in Burkina Faso (Ouattara et al., 2019) and Cote d'Ivoire (Soro et al., 2021). In September 2021, severe leaf yellowing, curling and deformation symptoms (Figure 1), resembling those of pepper yellow vein disease were observed on tobacco (Nicotiana tabacum) bordering pepper and tomato fields in the locality of Tabtenga in Burkina Faso. Tobacco leaves with (n = 3) and without (n = 2) symptoms were collected. The samples were tested for the presence of begomoviruses using a PCR assay with degenerate primers designed to amplify the coat protein gene of Old World begomoviruses (Séka et al., 2016) followed by direct sequencing of PCR amplicons. PCR products of the expected size were only obtained from the three diseased tobacco plants. BLASTn analyses of the amplicon sequences (676, 687 and 694 base pairs (bp) in length; GenBank Accession Nos. OR483371 - OR483373) showed the highest pairwise identity (98 to 99%) with PepYVMLV isolates from Burkina Faso (MH778652; MH778653). To confirm these results, specific primers for the detection of both DNA-A and -B components of PepYVMLV were used for PCR testing (Ouattara et al., 2019). Both components were detected from the three diseased samples confirming the initial diagnosis. To obtain the complete sequences of DNA-A and -B components, samples were subjected to nanopore MinION sequencing as described by Ben Chehida et al. (2021). Global similarity search analysis resulted in 84/3076 and 99/3076 raw reads assigned to PepYVMLV DNA-A and DNA-B, respectively. Based on sequence assembly only one contig of 2781 bp (DNA-A component) was obtained (OR483374) with 96.7% identity to PepYVMLV isolated from Burkina Faso (MH778653, FN555171). A maximum-likelihood phylogenetic tree constructed with publicly available begomovirus genome sequences and the sequences obtained in this study (OR483371 - OR483374) confirmed the genetic relationship of the isolates of PepYVMLV from tobacco with previously characterised isolates from West Africa (Figure 2). To our knowledge, this is the first report of PepYVMLV naturally associated with tobacco yellow leaf curl disease in Burkina Faso and globally. Our results highlight the potential existence of alternative natural hosts for PepYVMLV. This work was supported by The World Academy of Sciences, Trieste, Italy (TWAS) (Grant n°_21-147 RG/BIO/AF/AC_I-FR3240319486), the European Union (ERDF), the Conseil Régional de La Réunion and CIRAD, and conducted on the Plant Protection Platform (3P, IBISA). Dr Ouattara is a recipient of a 12-month visiting scientist fellowship of the program Make Our Planet Great Again (MOPGA-postdoc4-4610922765) from the French Ministry for Europe and ","PeriodicalId":36931,"journal":{"name":"New Disease Reports","volume":"99 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135606839","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Leaf spot of Ficus maclellandii caused by Penicillium crocicola ‐ a first report from Iran 由青霉菌（Penicillium crocicola）引起的榕树叶斑病--伊朗的首次报告

New Disease Reports Pub Date : 2023-10-01 DOI: 10.1002/ndr2.12238

A. Esmaeilzadeh, D. Zafari, M. Ketabchi

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First report of Neopestalotiopsis rosae causing leaf spot and crown rot on strawberry (Fragaria × ananassa) in Paraguay 在巴拉圭首次报告 Neopestalotiopsis rosae 在草莓（Fragaria × ananassa）上引起叶斑病和冠腐病

New Disease Reports Pub Date : 2023-10-01 DOI: 10.1002/ndr2.12239

Y. A. Fernández‐Ozuna, A. R. Gini Álvarez, H. Lopez-Nicora, A. A. Arrúa Alvarenga, A. A. Colmán

引用次数: 0

First report of strawberry anthracnose caused by Colletotrichum nymphaeae in Egypt 埃及草莓炭疽病报道首例

New Disease Reports Pub Date : 2023-07-01 DOI: 10.1002/ndr2.12205

T. M. Abo‐Elwafa, S. S. Ragab, Y. Nehela, T. A. Essa

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First report of Phytopythium vexans and Phytopythium litorale associated with root rot symptoms on red raspberry (Rubus idaeus) 与红树莓（Rubus idaeus）根腐症状相关的叶腐霉和李腐霉的首次报道

New Disease Reports Pub Date : 2023-07-01 DOI: 10.1002/ndr2.12197

E. Y. Browne, S. Edwards, C. Nellist

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First report of an aggressive species of Neopestalotiopsis affecting strawberry in Canada 加拿大首次报道了一种影响草莓的新estestalotiopsis侵染种

New Disease Reports Pub Date : 2023-07-01 DOI: 10.1002/ndr2.12210

J. McNally, K. Prapagar, K. Goldenhar, E. Pate, S. Shan, M. Kalischuk

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First report of Xanthomonas euvesicatoria pv. euvesicatoria causing bacterial leaf spot in chilli pepper (Capsicum sp.) in Indonesia 黄单胞菌pv。印度尼西亚辣椒（Capsicum sp.）中引起细菌性叶斑的euvesicatoria

New Disease Reports Pub Date : 2023-07-01 DOI: 10.1002/ndr2.12208

D. Utami, N. N. S. Jayasanti, S. Meale, A. Young

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First report of Pepo aphid‐borne yellows virus on watermelon plants in Spain 西班牙西瓜蚜传黄病毒首例报道

New Disease Reports Pub Date : 2023-07-01 DOI: 10.1002/ndr2.12215

C. De Moya‐Ruiz, M. Juárez, P. Gómez

{"title":"First report of Pepo aphid‐borne yellows virus on watermelon plants in Spain","authors":"C. De Moya‐Ruiz, M. Juárez, P. Gómez","doi":"10.1002/ndr2.12215","DOIUrl":"https://doi.org/10.1002/ndr2.12215","url":null,"abstract":"In 2021, fifty-two watermelon (Citrullus lanatus) samples exhibiting yellowing virus-like symptoms (Figures 1, 2) were collected from thirteen open-field plots in Murcia (southeastern Spain). Total RNA extraction and dot-blot molecular hybridization were performed to detect Cucumber vein yellowing virus, Cucurbit aphid-borne yellows virus (CABYV), Cucurbit chlorotic yellows virus, Cucurbit yellow stunting disorder virus and Zucchini yellow mosaic virus (Rabadán et al., 2023), but none of these viruses were detected. Leaf yellowing symptoms may also be confused with plant nutrient deficiency. However, considering the large aphid populations in the affected crops and the inconspicuous symptoms that poleroviruses often cause in affected cucurbits, we sought to check for the possibility of polerovirus infection. Five random samples were subjected to RT-PCR using the polerovirus primers PolGenUp2 and PolGen RT3 (Lotos et al., 2014). For three samples, a 593 bp amplicon was produced, encompassing part of the polerovirus RNA-dependent RNA polymerase (RdRp) and sequenced (STAB VIDA, Portugal). The sequencing results from the three amplicons confirmed the presence of a polerovirus and identified the virus as Pepo aphid-borne yellows virus (PABYV), with 96.7% nucleic acid identity to GenBank Accession No. LN865082, reported from Greece. PABYV is a phloem-restricted RNA plant viruses that is transmitted by aphids and has been detected in West Africa and the Mediterranean, including Greece, Italy and Syria (Lotos et al., 2016; Parrella et al., 2023). To understand the extent and distribution of PABYV in these watermelon production areas, we amplified the coat protein (CP) from a Spanish PABYV isolate as described by Masika et al. (2022). The CP was cloned into the pGEMT-easy vector to facilitate the synthesis of a specific RNA-probe labeled with digoxigenin. All the watermelon samples collected in 2021, and a further 68 symptomatic samples collected during the 2022 and 2023 seasons, were tested for PABYV by dot-blot molecular hybridization, as described by De Moya-Ruiz et al. (2021). The analysis revealed that the occurrence of PABYV ranged from 75 to 100% in watermelon crops during the three seasons. Dot-blot hybridization was also used to detect the other cucurbit viruses listed above. Only PABYV was detected in 2021 and 2022, while PABYV and CABYV were found in mixed infections in all samples from 2023. Three PABYV isolates per year were selected randomly, and the CP gene was amplified, sequenced and deposited in GenBank (OR253793-OR253801). Phylogenetic analysis was done using the maximum likelihood method in MEGA X. The nine PABYV Spanish isolates clustered in a group along with the Greek (LN865082), Italian (OP973153) and Syrian (KC960436) isolates (Figure 3). This would suggest a common origin for the PABYV isolates spreading in the Mediterranean area. This is the first report of PABYV in Spain. The emergence of this aphid-borne virus in a new geograp","PeriodicalId":36931,"journal":{"name":"New Disease Reports","volume":"55 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135805127","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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