New Disease Reports最新文献_第4页

First report of Tamarillo fruit ring virus in Solanum spp. in Uganda, Tanzania and Rwanda 首次报告在乌干达、坦桑尼亚和卢旺达的茄科植物中发现玉蜀黍果环病毒

New Disease Reports Pub Date : 2024-01-01 DOI: 10.1002/ndr2.12256

K. Alraiss, A. Fowkes, L. Frew, G. Webster, A. Skelton, C. Conyers, B. Barrett, I. Adams, S. McGreig, M. Mynett, K. Chisnall, R. Ward, M. Dixon, S. Forde, Z. Golding, J. Bailey, V. Pugliese, S. K. Waziri, T. Alicai, A. Fox

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First report of Ti ringspot‐associated virus infecting peach in Greece 希腊首次发现感染桃子的蒂环斑相关病毒

New Disease Reports Pub Date : 2024-01-01 DOI: 10.1002/ndr2.12240

D. Beris, I. Malandraki, C. Varveri

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The first report of Alternaria cantlous causing leaf spot on lentil (Lens culinaris) 引起扁豆叶斑病的cantlous Alternaria (Lens culinaris)首次报道

New Disease Reports Pub Date : 2023-10-01 DOI: 10.1002/ndr2.12220

M. E. A. Kouadri, A. A. Bekkar, S. Zaim

{"title":"The first report of Alternaria cantlous causing leaf spot on lentil (Lens culinaris)","authors":"M. E. A. Kouadri, A. A. Bekkar, S. Zaim","doi":"10.1002/ndr2.12220","DOIUrl":"https://doi.org/10.1002/ndr2.12220","url":null,"abstract":"During a survey in May 2019, lentil (Lens culinaris cv. Syria 229) plants cultivated in an open field in Tiaret, Algeria showed chlorotic leaf spots with an incidence of about 10%. The symptoms began as small, irregular lesions and expanded with time from the edge to the centre of the leaves. To identify the causal agent, diseased leaves were disinfected in 2% NaOCl for three minutes, rinsed thrice with sterile water, plated onto potato dextrose agar and incubated at 25±1°C for seven days. Fungal colonies were dark grey with dense growth. Conidiophores were brown, erect, simple or branched, 2.5-3.75 × 21–42.5 μm. Conidia were mostly ovoid or ellipsoidal, light to dark brown with 1 to 3 transverse septa and 0 to 2 longitudinal septa and ranged in size from 8.75-17.5 × 7.5-15 μm (n = 25) (Fig. 1). Based on morphological characters, isolates were identified as Alternaria sp. (Simmons, 2007). To confirm the identity of the fungus, a PCR was done to amplify ITS and tef1 regions of a representative isolate, ST2 (White et al., 1990; Carbone & Kohn, 1999). The obtained sequences (GenBank Accession Nos. OQ256241, OQ269665) were analysed with BLASTn and had 99.58 and 99.52% identity, 479/481(99%) and 209/210 (99%) pairs matching, with the ex-type sequences of Alternaria cantlous (CBS 123007) for ITS (KC584245) and tef1 (KC584739), respectively. Phylogenetic analysis of concatenated sequences of ITS and tef1 using the maximum likelihood method, clustered isolate ST2 consistently with the ex-type strain of Alternaria cantlous (Yong Wang bis & X.G. Zhang) (Woudenberg et al., 2013), with bootstrap support of 96% (Fig. 2). Based on the BLAST search and phylogenetic analysis, isolate ST2 was identified as Alternaria cantlous. A pathogenicity test was performed under greenhouse conditions by spraying a conidial suspension of 105 conidia/ml onto mature leaves of three lentil (cv. Syria 229) plants. Control plants were sprayed with sterile water. All plants were covered with a transparent plastic bag for 72 hours. After 20 days, the inoculated plants showed similar symptoms to those observed in the field (Fig. 3), while no symptoms were observed on control plants. The fungus was reisolated from all inoculated plants to fulfill Koch's postulates. To the best of our knowledge this is the first report of A. cantlous causing leaf spot on lentil in Algeria and worldwide. The authors wish to express their sincerest gratitude to Professor Madani Benouycef from University Mustapha Stambouli of Mascara, Department of Earth Sciences for providing the necessary facilities and equipment for the research work.","PeriodicalId":36931,"journal":{"name":"New Disease Reports","volume":"105 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135656202","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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First report of Colletotrichum liriopes on Liriope muscari (lily turf) in the United Kingdom 英国百合草皮上炭疽菌首次报道

New Disease Reports Pub Date : 2023-10-01 DOI: 10.1002/ndr2.12222

A. Ozolina, C. J. Field, H. M. Beaton, C. Cunningham, A. V. Barnes

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Clonostachys chloroleuca: A novel pathogen causing cassava root rot disease in Misiones Province, Argentina Clonostachys chloroleuca：导致阿根廷米西奥内斯省木薯根腐病的新型病原体

New Disease Reports Pub Date : 2023-10-01 DOI: 10.1002/ndr2.12229

L. M. Madrassi, R. D. González, C. I. Mónaco, P. Zapata, A. E. Alvarenga

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Stenotrophomonas sp. associated with offshoot decline of date palm in Iran 与伊朗枣椰树枝条衰退有关的 Stenotrophomonas sp.

New Disease Reports Pub Date : 2023-10-01 DOI: 10.1002/ndr2.12228

A. Abedinzadeh, N. Falahi Charkhabi, M. Aeini

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First report of Diplodia seriata associated with branch canker dieback on Cupressus sempervirens in Tunisia 突尼斯首次报告与半柏松枝干腐烂枯死有关的 Diplodia seriata

New Disease Reports Pub Date : 2023-10-01 DOI: 10.1002/ndr2.12234

S. Hlaiem, I. Yangui, H. Khadraoui, M. Jamâa, O. Ezzine

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First report of Plasmopara sphagneticolae causing downy mildew on bhringraj (Eclipta alba) in India 印度引起白霜霉病的白霜霉浆菌首次报道

New Disease Reports Pub Date : 2023-10-01 DOI: 10.1002/ndr2.12221

P. Sonavane, K. Hima Bindu, V. Venkataravanappa, S. Sriram, None Shivakumar

{"title":"First report of Plasmopara sphagneticolae causing downy mildew on bhringraj (Eclipta alba) in India","authors":"P. Sonavane, K. Hima Bindu, V. Venkataravanappa, S. Sriram, None Shivakumar","doi":"10.1002/ndr2.12221","DOIUrl":"https://doi.org/10.1002/ndr2.12221","url":null,"abstract":"Bhringraj (false daisy, family Asteraceae) is a valuable herb commonly used in ayurvedic and traditional medicine in the Indian subcontinent (Jahan et al., 2014); the leaf extract has antibacterial, antifungal and antiviral activity (Dalal et al., 2010). In experimental plots of false daisy at the ICAR-Indian Institute of Horticultural Research in Bengaluru, Karnataka, India, symptoms of severe downy growth were observed in September 2022 (post monsoon season). In the initial stage of infection, leaves showed yellowing and necrosis on adaxial surfaces and sparse mycelia growth was observed on the abaxial surface of older leaves. Later, thick white mycelial growth was observed on the lower side of young leaves (Figure 1). As the disease progressed, leaves dried and turned brown, then blackened, leading to 80–100 % loss in yield. One infected leaf was collected from each of three plants for further examination and pathogen identification. Microscopic observation revealed downy growth on the abaxial surface of leaves with numerous sporangia. The mycelium was hyaline, aseptate and branched, 5–15 μm in diameter. Sporangiophores were slender with monopodial branching at right angles and with a slight bulbous base. The sporangia were hyaline, smooth, globose to ellipsoidal with apical papilla and basal hilum on three sterigma, measuring 18–30 × 14–20 µm (Figure 2). Based on these characteristics, the pathogen was identified as Plasmopara sphagneticolae (Davis et al., 2020; Wang et al., 2021). To confirm the identity of the pathogen, total DNA was isolated from two of the samples (PSB-1 and PSB-2) using the CTAB method and the COX 2 region was amplified using primers Cox2F and Cox2R (Hudspeth et al., 2000). Analysis of the sequences using BLASTn showed the highest identity to P. sphagneticolae isolates BRIP61010 and BPI919166 (Genbank Accession Nos. KM085175 and MT292790, respectively). The consensus sequences were submitted to Genbank (OQ200475 and OQ200476). Phylogenetic analysis showed that the sequences of PSB-1 and PSB-2 clustered closely with isolates of P. sphagneticolae. Both P. sphagneticolae and its sister species P. halstedii cause downy mildew on many crops in the Asteraceae. Plasmopara sphagneticolae was first identified in Australia infecting Sphagenticola trilobata (McTaggart et al., 2015), and was then found on Lipochaeta integrifolia in Hawaii (Davis et al., 2020) and Taiwan (Wang et al., 2021). This is the first report of P. sphagneticolae in India and the first record of this pathogen causing downy mildew on bhringraj. The authors are grateful to the Director, Indian Institute of Horticultural Research, Bangalore. Financial assistance to K. Hima Bindu from NMPB, India (Project No; Z. 18017) is acknowledged.","PeriodicalId":36931,"journal":{"name":"New Disease Reports","volume":"12 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136054899","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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First report of quinone outside inhibitor stigmatellin binding type (QoSI) resistance in Plasmopara viticola in India 印度葡萄浆原菌对醌外抑制剂柱头蛋白结合型(QoSI)的耐药性首次报道

New Disease Reports Pub Date : 2023-10-01 DOI: 10.1002/ndr2.12223

N. Sagar, M. M. Jamadar, C. N. L. Reddy, B. R. Sayiprathap, M. Bharath, N. H. Shalini, S. B. Jagginavar, P. S. Pattar, C. R. Jahir Basha

{"title":"First report of quinone outside inhibitor stigmatellin binding type (QoSI) resistance in Plasmopara viticola in India","authors":"N. Sagar, M. M. Jamadar, C. N. L. Reddy, B. R. Sayiprathap, M. Bharath, N. H. Shalini, S. B. Jagginavar, P. S. Pattar, C. R. Jahir Basha","doi":"10.1002/ndr2.12223","DOIUrl":"https://doi.org/10.1002/ndr2.12223","url":null,"abstract":"Grape (Vitis vinifera) is an important fruit crop in India with about 3.5 million tonnes produced annually from an area of 111,000 ha. The Indian state of Karnataka is the major producer of grapes contributing about 25% of the national production. Downy mildew disease caused by Plasmopara viticola (Berk. & Curt.) is a threat to grape production throughout the world. Several fungicides have been used to manage downy mildew in grapevine, the most important being the quinone outside inhibitor stigmatellin binding type (QoSI) fungicide, ametoctradin, which has been used successfully in India for about a decade. However, in recent years’ farmers have noticed the failure of ametoctradin in managing downy mildew, and evolved resistance has been observed in France (Fontaine et al., 2019). In the present study, 41 downy mildew samples were collected from the major grapevine-growing districts of Karnataka state during 2022–23 and analysed for the presence of QoSI resistance. Sensitivity to QoSI fungicide was determined using a modified 24-well leaf-disc bioassay (Fungicide Resistance Action Committee, 2003). Healthy leaves were taken from the 6th node from the apex of a growing shoot of a downy mildew-susceptible grapevine cultivar (cv. Thomson Seedless) and 15 mm discs were cut from the leaves. The leaf discs were placed upside down in wells containing 1 mL of 0.5% water agar amended with 0, 100, 500, 1500, 2500 or 3000 µg mL−1 of either ametoctradin (300 g/l active ingredient) or dimethomorph (225 g/l active ingredient) (Zampro, BASF) as described by Sawant et al. (2016). Each treatment was repeated four times. Leaf disks were then inoculated with 10 µL of a suspension of P. viticola sporangia (50,000 sporangia mL−1) collected from a single lesion. Plates were incubated at 22°C with alternating periods of 12 hours light and dark. After six days, lesion area was measured and EC50 value was calculated by regression analysis of the per cent area of infection versus log10 fungicide concentration and resistance factor was also determined as described by Massi et al. (2021). The EC50 value of a sensitive isolate to the ametoctradin and dimethomorph mix was 2.36 µg/mL. The EC50 value of moderately resistant isolates ranged from 389 to 874 g/mL with RF (164-370), for resistant isolates the value ranged from 569 to 1126 µg/mL with RF (241-477) and for highly resistant isolates it ranged from 956 to 1423 µg/mL with RF (405-603). The BN-2 isolate from Babanagar, Vijayapur district had the largest EC50 value (1423 µg/mL). The resistance to QoSI fungicides in P. viticola developed due to a mutation in the cytochrome b (Pv-Cyt b) gene at the S34L site. This was detected using allele-specific primers 5′-ATTATTTTTATGGATTCGGTTT-3′ (forward) and 5′-ACCAACCGTTATTTACATCAC-3′ (reverse) as described by Fontaine et al. (2019). Total DNA was isolated from the isolates using a NucleoSpin Plant II kit as per the manufacturer's protocol (Macherey-Nagel, Germany) and the quality wa","PeriodicalId":36931,"journal":{"name":"New Disease Reports","volume":"54 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136093471","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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First report of Pineapple mealybug wilt‐associated virus 1 and 3 associated with mealybug wilt of pineapple disease on pineapple in Malaysia 马来西亚首次报告菠萝蚧枯萎病相关病毒 1 和 3 与菠萝蚧枯萎病有关

New Disease Reports Pub Date : 2023-10-01 DOI: 10.1002/ndr2.12231

I. Mohd Anuar, S. A. Nusaibah, G. Vadamalai, A. S. Mokhtar, Z. Sapak

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