Electrophoresis Letters最新文献

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Characteristic analysis of a secondary structure of primary microRNA bound to a double-strand RNA-binding protein, NF90 与双链rna结合蛋白NF90结合的初级microRNA二级结构的特征分析
Electrophoresis Letters Pub Date : 1900-01-01 DOI: 10.2198/ELECTROPH.65.13
Takuma Higuchi, R. Kiernan, S. Sakamoto
{"title":"Characteristic analysis of a secondary structure of primary microRNA bound to a double-strand RNA-binding protein, NF90","authors":"Takuma Higuchi, R. Kiernan, S. Sakamoto","doi":"10.2198/ELECTROPH.65.13","DOIUrl":"https://doi.org/10.2198/ELECTROPH.65.13","url":null,"abstract":"unclear. In this study, we attempted to characterize the structure of NF90 binding pri-miRNAs by informatics analysis and RNA-electrophoretic mobility shift assay (RNA-EMSA). We used genome-wide approaches including ENCODE and small RNA-seq to identify pri-miRNAs that are associated with NF90. As a result, we found that NF90-targerted pri-miRNAs are highly stable, having a lower free energy and fewer mismatches compared to all pri-miRNAs. To confirm this, we performed RNA-EMSA probed with mutated pri-miRNAs. Pri-miR-3173 and pri-miR-186, the NF90-targeted pri-miRNA, were mutated to unstable structure by making bulges in the stem structure, whereas pri-miR-200a, which exhibits low affinity to NF90, was changed to more stable structure by forming long duplex in the stem. Consequently, we found that the mutated pri-miR-3173 and pri-miR-186 lead to a reduction in binding activity of NF90 to the pri-miRNAs. On the other hand, the binding affinity of NF90 to pri-miR-200a was dramatically elevated by forming the long duplex in the structure. These results suggest that NF90 preferentially binds pri-miRNAs having less mismatches and highly stable stem structure.","PeriodicalId":369290,"journal":{"name":"Electrophoresis Letters","volume":"9 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128548568","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Trace omics analysis by ultra-sensitive capillary electrophoresis 超灵敏毛细管电泳的痕量组学分析
Electrophoresis Letters Pub Date : 1900-01-01 DOI: 10.2198/electroph.64.23
T. Kawai
{"title":"Trace omics analysis by ultra-sensitive capillary electrophoresis","authors":"T. Kawai","doi":"10.2198/electroph.64.23","DOIUrl":"https://doi.org/10.2198/electroph.64.23","url":null,"abstract":"Firstly, small number of cells (1–100) were manually collected, and they were chemically and/or enzymatically processed in a PCR tube with a micropi-pette. The obtained sample solution was then introduced into a capillary, preconcentrated by LDIS, electrophoretical-ly separated, and detected by highly sensitive detector like laser-induced fluorescence (LIF) or mass spectrometry (MS). The obtained data were processed via a database search and finally metabolome and glycome analyses were achieved for 1–100 cells.","PeriodicalId":369290,"journal":{"name":"Electrophoresis Letters","volume":"77 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134058736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Development of a fully automated enzymatic lipoprotein fractionation method for staining total cholesterol and triglycerides 一种全自动酶促脂蛋白分离方法的发展,用于染色总胆固醇和甘油三酯
Electrophoresis Letters Pub Date : 1900-01-01 DOI: 10.2198/electroph.65.69
M. Matsushita, Natsumi Yamaguchi, Marina Tanaka
{"title":"Development of a fully automated enzymatic lipoprotein fractionation method for staining total cholesterol and triglycerides","authors":"M. Matsushita, Natsumi Yamaguchi, Marina Tanaka","doi":"10.2198/electroph.65.69","DOIUrl":"https://doi.org/10.2198/electroph.65.69","url":null,"abstract":"","PeriodicalId":369290,"journal":{"name":"Electrophoresis Letters","volume":"20 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131757394","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Trajectory of autoantibody-based cancer biomarker research 基于自身抗体的癌症生物标志物研究轨迹
Electrophoresis Letters Pub Date : 1900-01-01 DOI: 10.2198/electroph.66.43
Makoto Kobayashi, K. Sugimoto, H. Chiba
{"title":"Trajectory of autoantibody-based cancer biomarker research","authors":"Makoto Kobayashi, K. Sugimoto, H. Chiba","doi":"10.2198/electroph.66.43","DOIUrl":"https://doi.org/10.2198/electroph.66.43","url":null,"abstract":"","PeriodicalId":369290,"journal":{"name":"Electrophoresis Letters","volume":"100 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127067680","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Novel signaling pathways that regulate suppression of insulin gene expression in type 2 diabetes 调节2型糖尿病胰岛素基因表达抑制的新信号通路
Electrophoresis Letters Pub Date : 1900-01-01 DOI: 10.2198/electroph.65.47
Yasunori Sugiyama, Tatsuto Nakane, Shuji Sakmoto, K. Murao
{"title":"Novel signaling pathways that regulate suppression of insulin gene expression in type 2 diabetes","authors":"Yasunori Sugiyama, Tatsuto Nakane, Shuji Sakmoto, K. Murao","doi":"10.2198/electroph.65.47","DOIUrl":"https://doi.org/10.2198/electroph.65.47","url":null,"abstract":"","PeriodicalId":369290,"journal":{"name":"Electrophoresis Letters","volume":"54 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129466367","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Multi-omics analysis based on proteomics is fun! : development of proteogenomic software generating sample-specific proteome database and comprehensive kinase activity analysis 基于蛋白质组学的多组学分析很有趣!:开发蛋白质基因组软件,生成样品特异性蛋白质组数据库和全面的激酶活性分析
Electrophoresis Letters Pub Date : 1900-01-01 DOI: 10.2198/electroph.67.5
Rei Noguchi
{"title":"Multi-omics analysis based on proteomics is fun! : development of proteogenomic software generating sample-specific proteome database and comprehensive kinase activity analysis","authors":"Rei Noguchi","doi":"10.2198/electroph.67.5","DOIUrl":"https://doi.org/10.2198/electroph.67.5","url":null,"abstract":"","PeriodicalId":369290,"journal":{"name":"Electrophoresis Letters","volume":"35 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123827612","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
患者腫瘍移植(Patient derived xenograft; PDX)モデルバンクの樹立 患者肿瘤移植(Patient derived xenograft;PDX)建立示范银行
Electrophoresis Letters Pub Date : 1900-01-01 DOI: 10.2198/electroph.67.13
Ryusho Kariya, Seiji Okada
{"title":"患者腫瘍移植(Patient derived xenograft; PDX)モデルバンクの樹立","authors":"Ryusho Kariya, Seiji Okada","doi":"10.2198/electroph.67.13","DOIUrl":"https://doi.org/10.2198/electroph.67.13","url":null,"abstract":"","PeriodicalId":369290,"journal":{"name":"Electrophoresis Letters","volume":"11 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121206829","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Heat-denatured protein analysis by SDS-PAGE with low concentration of SDS. in the extraction medium 低浓度SDS- page热变性蛋白分析。在萃取介质中
Electrophoresis Letters Pub Date : 1900-01-01 DOI: 10.2198/electroph.66.59
M. Oh‐ishi, Sahara Watanabe, Kaimu Matsumoto
{"title":"Heat-denatured protein analysis by SDS-PAGE with low concentration of SDS. in the extraction medium","authors":"M. Oh‐ishi, Sahara Watanabe, Kaimu Matsumoto","doi":"10.2198/electroph.66.59","DOIUrl":"https://doi.org/10.2198/electroph.66.59","url":null,"abstract":"","PeriodicalId":369290,"journal":{"name":"Electrophoresis Letters","volume":"80 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126259073","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Pineal allopregnanolone saves cerebellar Purkinje cells from apoptosis 松果体异孕醇酮保护小脑浦肯野细胞免于凋亡
Electrophoresis Letters Pub Date : 1900-01-01 DOI: 10.2198/ELECTROPH.63.19
S. Haraguchi
{"title":"Pineal allopregnanolone saves cerebellar Purkinje cells from apoptosis","authors":"S. Haraguchi","doi":"10.2198/ELECTROPH.63.19","DOIUrl":"https://doi.org/10.2198/ELECTROPH.63.19","url":null,"abstract":"","PeriodicalId":369290,"journal":{"name":"Electrophoresis Letters","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115243514","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Diagnostic significance of NAP1L1 expression in patients with lung adenocarcinoma 肺腺癌患者NAP1L1表达的诊断意义
Electrophoresis Letters Pub Date : 1900-01-01 DOI: 10.2198/electroph.66.9
R. Nagashio, Yuki Kuchitsu
{"title":"Diagnostic significance of NAP1L1 expression in patients with lung adenocarcinoma","authors":"R. Nagashio, Yuki Kuchitsu","doi":"10.2198/electroph.66.9","DOIUrl":"https://doi.org/10.2198/electroph.66.9","url":null,"abstract":"","PeriodicalId":369290,"journal":{"name":"Electrophoresis Letters","volume":"4 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125547565","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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