Trace omics analysis by ultra-sensitive capillary electrophoresis

Firstly, small number of cells (1–100) were manually collected, and they were chemically and/or enzymatically processed in a PCR tube with a micropi-pette. The obtained sample solution was then introduced into a capillary, preconcentrated by LDIS, electrophoretical-ly separated, and detected by highly sensitive detector like laser-induced fluorescence (LIF) or mass spectrometry (MS). The obtained data were processed via a database search and finally metabolome and glycome analyses were achieved for 1–100 cells.