R. Nawaz, N. Abbasi, I. Hafiz, Z. Khan, Muhammad Riaz Khan, A. Khalid
{"title":"IMPACT OF PEEL COLORATION ON INTERNAL QUALITY OF KINNOW MANDARIN (Citrus nobilis Lour x Citrus deliciosa Tenora) AT EARLY RIPENING STAGE","authors":"R. Nawaz, N. Abbasi, I. Hafiz, Z. Khan, Muhammad Riaz Khan, A. Khalid","doi":"10.33865/WJB.004.01.0186","DOIUrl":"https://doi.org/10.33865/WJB.004.01.0186","url":null,"abstract":"At earlier maturation, Kinnow mandarin color-break fruits were analyzed for internal quality. Ten fruits were harvested from dark green to fully red or yellow colored at field on visual observation and arranged in order of dark green to deep red or yellow through Chroma meter after being numbered as 1 to 10. Triplicated determinations of all samples were analyzed. In red colored fruits juice, a decreasing trend of titratable acidity, ascorbic acid and TDS was found with increasing pattern of sugars, TSS and pH. Similarly in peel, less chlorophyll and more carotenoids were recorded in red colored fruits. Total phenolic contents in juice remained constant with declining trend in peel from green to red colored fruits. In juice both flavonoids and flavonols were constant while in peel flavonols increase was noted in red colored fruits. Total anthocyanins showed an increasing trend in red colored fruit for both, the peel and juice. Antioxidant activity was increased in juice and decreased in peel for red colored fruits. Nutritional values of green color peel fruits are alike those of red colored, but less sugars, reduced sweetness were duly noted regarding their domestic consumers acceptance. Green color fruits have more ascorbic acid, polyphenols and reducing sugars are equally valuable like red colored produce, which is especially good for the purpose of export","PeriodicalId":366978,"journal":{"name":"World Journal of Biology and Biotechnology","volume":"55 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115895048","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"VERMINOUS PNEUMONIA IN A CALF FROM A DAIRY FARM UNIT, A CASE STUDY","authors":"N. Nour, Salehi Alireza","doi":"10.33865/WJB.004.01.0196","DOIUrl":"https://doi.org/10.33865/WJB.004.01.0196","url":null,"abstract":"Following a report of a respiratory problem in a calf’s head with an approximate age of 60 days, a livestock unit has undergone basic examinations. Symptoms such as coughing, epistaxis and fever of 40°C were observed. Antibiotics along with serum were prescribed as treatment but the calf has been wasted after a week. With an autopsy and the lung examination, a huge amount of white and thick mucosal secretions as well as a number of parasite larvae have also been isolated within the bronchi. The morphological characteristics of these larvae have been confirmed by Dictyocaulus viviparus parasite infection. Basically, the role of parasitic agents in the development of pneumonia in calves is very significant. Due to the lesser consideration of the role of parasitic agents in the calf’s pneumonia and in addition to the high cost of treatment, this, ultimately leads to the death of calves. Therefore, in dealing with such cases, Para clinical tests such as stool test is also suggested","PeriodicalId":366978,"journal":{"name":"World Journal of Biology and Biotechnology","volume":"68 S25","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"113973187","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Kouser Majeed Malik, S. Y. Naz, S. M. Mehdi, A. Munir, Ahmad Khan, Saba Akbar, S. Andleeb, Z. Rehman
{"title":"EXPLORING DIFFERENT FERTILIZER COMBINATIONS FOR POTATO GROWTH AND YIELD IN RAWALPINDI REGION OF PAKISTAN","authors":"Kouser Majeed Malik, S. Y. Naz, S. M. Mehdi, A. Munir, Ahmad Khan, Saba Akbar, S. Andleeb, Z. Rehman","doi":"10.33865/WJB.003.03.0171","DOIUrl":"https://doi.org/10.33865/WJB.003.03.0171","url":null,"abstract":"Potato (Solanum tuberosum L.) is one of the most important tuber crops produced in Pakistan. Due to its nutritive importance, it ranks as fourth after rice, wheat and maize in the world. However, production of the crop is far below the average due to poor crop stand, soil fertility and water management practices. There is a strong need for improvement in the fair yield of potato through managed production techniques and best soil nutrient combinations. To find out the best combination of fertilizer level of NPK on growth, yield and quality of the potato crop, nine field experiments on potato crop were conducted at Rawalpindi division, Punjab Pakistan for three successive Rabi seasons during 2014-2017. Treatments consisted of four levels of N (244, 300, 356 and 412 Kg N ha-1), P (163, 200, 237 and 275 kg P ha-1) and K (136, 167, 198 and 229 Kg K ha-1) laid out in Randomized Complete Block Design (RCBD) with three replications. Results showed that there was no significant difference of location in terms of yield (T ha-1). However, mean data showed that the fertilizer combinations significantly (<0.05) affected yield and yield components of the potato crop. Among the treatments, NPK level (412, 275 and 229 Kg ha-1) gave maximum (100 tuber m-2), potato yield (3.2 Kg m-2), dry matter (1.3 Kg m-2), while the least number of tuber m-2 (63), potato yield m-2 (2.02 Kg), dry matter yield m-2 (0.9 Kg) were recorded where combination NPK (244, 163and 136 Kg ha-1) were applied. From the results, it can be concluded that farming community can obtain the maximum potato yield by adopting NPK (412, 275, 229) levels of fertilizers","PeriodicalId":366978,"journal":{"name":"World Journal of Biology and Biotechnology","volume":"2 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126883213","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Asim, E. Haque, T. Ashraf, Amina, A. Hayat, A. Aziz
{"title":"APPLICATION OF PLANT GROWTH REGULATOR AND POTASSIUM NITRATE TO IMPROVE THE QUALITY AND YIELD IN WASHINGTON NAVEL ORANGES (Citrus sinensis)","authors":"M. Asim, E. Haque, T. Ashraf, Amina, A. Hayat, A. Aziz","doi":"10.33865/WJB.003.03.0168","DOIUrl":"https://doi.org/10.33865/WJB.003.03.0168","url":null,"abstract":"Fruit yields of traditional navels like Washington Navels are lower as compared to other sweet oranges due to low fruit set and quality problems like creasing. Effect of plant growth regulator and Potassium (GA3 and KNO3) alone and in combination were tested in Sargodha, Punjab conditions at full bloom stage of Washington navel oranges. Impact of these treatments was tested on fruit yield, quality and to reduce the physiological disorder; incidence of creasing. Fruit set and yields were increased over non-treated trees, while combine treatment of GA3 and KNO3 (GA3 25% and KNO3 2%) had best results in fruit yields and quality i.e. fruit weight, size, TSS, acidity and other qualitative characteristics of the fruit. Creasing was effectively minimized by spraying plant in combination of GA3 and KNO3, thus reducing the quantitative and qualitative post-harvest fruit losses.","PeriodicalId":366978,"journal":{"name":"World Journal of Biology and Biotechnology","volume":"38 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128859965","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"EVALUATION OF DIFFERENT PRESERVATIVE SOLUTIONS AND PACKAGING MATERIAL FOR IMPROVING POST HARVEST QUALITY OF GLADIOLUS (Gladiolus grandiflorus) CUT SPIKES","authors":"A. Manzoor, A. Rahman, M. Qamar, Sharmin Ashraf","doi":"10.33865/WJB.003.03.0165","DOIUrl":"https://doi.org/10.33865/WJB.003.03.0165","url":null,"abstract":"Gladiolus is an important cut flower in the world, and its preference in Pakistan is next to roses. But the main problem that occurs in gladiolus is that it has short vase life. The present experiment was conducted with an objective to investigate the effect of four preservative solutions [distilled water, Sucrose (3%), AgNO3 (250 ppm), AgNO3 (250 ppm) + Sucrose (3%)] and packaging material on postharvest quality of three gladiolus varieties, “Tissue, White Prosperity and Alexandra”. Packaging material consists of control treatment (without packaging), packaging of cut spikes with 100 ppm acetic acid soaked cotton and packaging of polyethylene sheet after sucrose pulsing. The experiment was arranged as two factorial laid out according to completely randomized design (CRD) having three replications. Results showed that preservative solution having combination of AgNO3 (250 ppm) + Sucrose (3%) significantly improves days to open basal floret, floret opening percentage (%), bloom spread (inch), floret length (inch), floret diameter (inch), fresh spike weight (g), dry spike weight (g), fresh weight loss (%) and vase life (days). Moreover, this treatment was also effective in reducing the wilting (%) in all gladiolus varieties. However, for solution uptake (mL/spike) and solution balance (ml/spike), AgNO3 (250 ppm)alone gives the best results. For packaging treatment, the pulsing of a cut spike with 20% sucrose followed by polyethylene sheet wrapping proves to be effective. Among the varieties, Alexandra performed better for all quality parameters in comparison to White Prosperity and Tissue","PeriodicalId":366978,"journal":{"name":"World Journal of Biology and Biotechnology","volume":"44 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133948364","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Shafiq, R. Fatima, S. Mushtaq, H. M. Salman, M. Talha, Saira Razaq, M. Haider
{"title":"MOLECULAR CHARACTERIZATION OF ASIAN CITRUS PSYLLID (Diaphorina citri) USING MITOCHONDRIAL CYTOCHROME OXIDASE 1 (MTCO1) GENE FROM PUNJAB PAKISTAN","authors":"M. Shafiq, R. Fatima, S. Mushtaq, H. M. Salman, M. Talha, Saira Razaq, M. Haider","doi":"10.33865/WJB.003.03.0170","DOIUrl":"https://doi.org/10.33865/WJB.003.03.0170","url":null,"abstract":"Citrus greening caused by (Candidatus liberibacter spp.) is one of the most destructive bacterial disease of citrus all over the world and it is transmitted by grafting and psyllid (Diaphorina citri) is responsible for its spread. This study was aimed to detect the prevalence of CLas in different varieties of citrus in Pakistan and molecular characterization of mtco1 gene in Diaphorina citri. DNA was extracted from fourteen different citrus cultivars infected with citrus greening from different orchards of Sargodha Punjab, Pakistan. Specific primers were used to amplify (Mtco1 gene) in Diaphorina citri and also Las specific primers were also used to detect HLB pathogen. Amplified fragments of 507bp, 1500bp and 2500bp were observed in CLas and Mtco1 gene of Asian isolates. The resulted fragments were TA cloned and sequenced from both strands. The infectious bacterium was found in 3 samples out of 14 (21.5 %) while from psyllid it was found in 5 samples (37.5%). The weather changes effect the prevalence of CLas in citrus but usually it decrease during autumn and found Las positive vector in spring or summer season up to 42oC. So there is a need to research on management of citrus greening disease in Pakistan as it caused major yield loses","PeriodicalId":366978,"journal":{"name":"World Journal of Biology and Biotechnology","volume":"25 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125054592","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Shabir Ahmed, M. Asim, T. Ashraf, A. Bakhsh, E. Haque, A. Hayat, M. N. Khan
{"title":"PERFORMANCE OF MUSAMBI SWEET ORANGE ON ROUGH LEMON AND VOLKAMERIANA ROOTSTOCKS AT SARGODHA, PAKISTAN","authors":"Shabir Ahmed, M. Asim, T. Ashraf, A. Bakhsh, E. Haque, A. Hayat, M. N. Khan","doi":"10.33865/WJB.003.03.0167","DOIUrl":"https://doi.org/10.33865/WJB.003.03.0167","url":null,"abstract":"Musambi (Citrussinensis) is the commercial variety of the sweet oranges group well flourishing inPakistan. Formany years Musambi has beenon the way of rapid decline with reduced life span besides drastic reduction in yield and fruit quality grafted on rough lemon rootstock. To overcome this problem, Volkameriana as an alternative rootstock to rough lemon was tested with the focus to find out more compatible rootstock to prevent mentioned issues. Observations on different plant growth parameters such as plant height, stock and scion circumference, canopy volume, plant fruit yield and important quality characteristics of fruit were made. The rough lemon depicted statistically significant result in plant height, spread and canopy volume in responses recorded for 10 years consecutively from 2008 to 2017. The degree of compatibility in both rootstocks in the year 2008 to 2009 were statistically non-significant. The yield was measured in terms of average No. of fruits per plant depicted that Rough Lemon and Volkameriana rootstock produced average 362.62 and 228.04 No. of fruits per plant respectively for the years 2012 to 2017. Moreover, results in different important quality characters i.e. fruit weight (g), Peel thickness (mm), Juice percentage, TSS and acidity were found better in the case of the plants grafted on Rough Lemon with the mean values of 176.23, 3.35, 46.56, 10.51 and 0.41respectively for the six years consecutive 2012-2017. Results of studied rootstocks with context to given parameters remained at par; so other promising rootstocks should be taken in account for further studies","PeriodicalId":366978,"journal":{"name":"World Journal of Biology and Biotechnology","volume":"76 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123020472","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Sharmin Ashraf, A. Manzoor, Bushra Zulfiqar, M. Tariq
{"title":"EFFECT OF CYTOKININS ON IN VITRO SHOOT PROLIFERATION OF OLIVE CULTIVARS “EARLIK” AND “BARI ZAITOON-2”","authors":"Sharmin Ashraf, A. Manzoor, Bushra Zulfiqar, M. Tariq","doi":"10.33865/wjb.003.02.0156","DOIUrl":"https://doi.org/10.33865/wjb.003.02.0156","url":null,"abstract":"In olive, micro-propagation is an effective technique for mass multiplication of disease free, true to type plants, but shoot proliferation in mature tissues of olive is a major difficulty encountered during culture establishment. Thus an experiment was designed with an objective to study the effect of different cytokinins (BAP and Zeatin) on shoot proliferation of two olive cultivars (Earlik and BARI Zaitoon-2). In olive Rugini medium, BAP and Zeatin were added alone and in combination at a concentration of 1 mg L-1 and 2 mg L-1. Experiments were arranged according to completely randomized design (CRD) with three replications per treatment. Results showed that both the olive cultivars performed well when BAP + Zeatin were supplemented to the medium at 2+2 mg L-1 concentration. However, the cultivar “BARI Zaitoon-2” had the highest shooting percentage (90.67 %) and number of nodes per shoot (8.33) in treatment containing BAP (2 mg L-1) + Zeatin (2 mg L-1), whereas, “Earlik” at same concentration had more shoot length (9.10 cm) and number of shoots per explant (3.67). Thus, it was concluded that the findings of these results will help in the future for developing shoot proliferation protocols for other olive cultivars. Moreover, these protocols can also be further be used in other woody plants micro propagation","PeriodicalId":366978,"journal":{"name":"World Journal of Biology and Biotechnology","volume":"14 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122078370","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Abid, Muhammad Umer Khan, S. Mushtaq, S. Afzaal, M. Haider
{"title":"A COMPREHENSIVE REVIEW ON MYCOVIRUSES AS BIOLOGICAL CONTROL AGENT","authors":"M. Abid, Muhammad Umer Khan, S. Mushtaq, S. Afzaal, M. Haider","doi":"10.33865/WJB.003.02.0146","DOIUrl":"https://doi.org/10.33865/WJB.003.02.0146","url":null,"abstract":"Mycoviruses are viruses that infect fungi. They have been reported in all major taxa of fungi. Generally extracellular phase is not present in mycoviruses and are transmitted during cell division intracellularly in sporogenesis, and/or hyphal anastomosis. The genome of mycoviruses has dsRNA, but a number of positive- or negative-strand ssRNA and ssDNA viruses have been isolated and characterized. There is an increasing interest for use of mycoviruses as novel biocontrol agent as they reduce the virulence of their phytopathogeinc fungal hosts. Mycoviruses can also infect endophytic fungi and encode killer toxins. Better understanding of mycoviruses assembly, function, and evolution have been done through their structural analyses. Recent report on virus taxonomy suggested that mycoviruses genome mostly consists of double-stranded RNA (dsRNA), and about 30% of mycoviruses genome is composed of a positive, single-stranded RNA (+ssRNA). Recently a mycovirus that is related to Gemini viruses has been reported. Scientists have reported mycoviruses in Chytridiomycota, Zygomycota, Ascomycota, Deuteromycota, and Basidiomycota. Many mycoviruses and their fungal hosts still remain unknown recently developed metagenomics approaches will be useful for detecting and identifying new mycoviruses","PeriodicalId":366978,"journal":{"name":"World Journal of Biology and Biotechnology","volume":"4 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132181686","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. Mushtaq, M. Shafiq, F. Khan, T. Ashraf, M. Haider
{"title":"EFFECT OF BACTERIAL ENDOPHYTES ISOLATED FROM THE CITRUS ON THE PHYSICAL PARAMETER OF BITTER GOURD (Momordica charantia L.)","authors":"S. Mushtaq, M. Shafiq, F. Khan, T. Ashraf, M. Haider","doi":"10.33865/WJB.003.02.0151","DOIUrl":"https://doi.org/10.33865/WJB.003.02.0151","url":null,"abstract":"Bacterial endophytes have a symbiotic relationship between different parts of the plant and could play an important role to improve plant health. The effects of endophytic bacterial communities in bitter gourd have not been studied yet in Pakistan. In the present study, ten different bacteria that belong to class Alpha, beta and gamma Proteobacteria as well as bacilli were injected into bitter gourd seedlings under glass house conditions and five weeks after physical parameters (SL, RL, leaf area, leaf number, wet and dry weight of plant, root and shoot ratio) were studied. According to results Azomonas agilis and Ensifer adhaerens give good results as compared to control for all parameters studied. These inoculants could be used as bio fertilizer for sustainable crop production system.","PeriodicalId":366978,"journal":{"name":"World Journal of Biology and Biotechnology","volume":"71 2 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132363535","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}