Journal of Traditional Chinese Medical Sciences最新文献

{"title":"Potential benefits of Qi Gong meditation in quantifiable physiology: A five-year longitudinal observation","authors":"Ashley Xia","doi":"10.1016/j.jtcms.2022.06.005","DOIUrl":"https://doi.org/10.1016/j.jtcms.2022.06.005","url":null,"abstract":"<div><h3>Objective</h3><p>Qi Gong is a mind-and-body intervention to improve health and relieve disease conditions, which has been practiced for thousands of years. Lack of quantitative measures to monitor the quality and progress of Qi Gong practice has hindered the high-quality study to evaluate its health benefits.</p></div><div><h3>Methods</h3><p>To develop such quantitative measures, vital sign changes in Qi Gong meditation practice of a single individual were monitored for 5 years by wearable devices.</p></div><div><h3>Results</h3><p>This retrospective and exploratory analysis revealed significant changes in blood oxygen saturation, pulse rate, respiration rate, and perfusion index in response to Qi Gong practice and physiological adaptation to its long-term training. Among the physiological changes in vital signs, a physiological state with a significant 2-fold increase in pulse-respiration rate ratio was detected during Qi Gong meditation while the ratios were maintained at 4 under resting, sleeping, and mild cycling conditions.</p></div><div><h3>Conclusion</h3><p>This is an inaugural report of a five-year longitudinal observation of this kind. Once validated in a well-designed cohort study, these non-invasive and easily obtained vital signs may be used as biomarkers to assess the adherence to breathing control during Qi Gong practice and can be applied to quantify the quality of Qi Gong practice in clinical trials with Qi Gong intervention.</p></div>","PeriodicalId":36624,"journal":{"name":"Journal of Traditional Chinese Medical Sciences","volume":"9 3","pages":"Pages 340-348"},"PeriodicalIF":0.0,"publicationDate":"2022-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2095754822000539/pdfft?md5=415ed173722e35de52e2f181c8fe600c&pid=1-s2.0-S2095754822000539-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72243945","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Common traditional Chinese medicine therapies for diarrhea-predominant irritable bowel syndrome: An overview of systematic reviews","authors":"Jun Zhao ,&nbsp;Xia Li ,&nbsp;Xiaowei Chen ,&nbsp;Hui Zheng ,&nbsp;Kun Ye ,&nbsp;Yunzhou Shi ,&nbsp;Xin Xie ,&nbsp;Jianfang Wang ,&nbsp;Min Chen ,&nbsp;Zhigang Li","doi":"10.1016/j.jtcms.2022.06.003","DOIUrl":"10.1016/j.jtcms.2022.06.003","url":null,"abstract":"<div><h3>Objective</h3><p>To critically evaluate and summarize the methodological quality of systematic reviews (SRs) and present objective and important outcomes on the effectiveness of traditional Chinese medicine (TCM) therapies, including Chinese herbal medicine (CHM), acupuncture, and moxibustion, for diarrhea-predominant irritable bowel syndrome (IBS-D).</p></div><div><h3>Methods</h3><p>We conducted a comprehensive literature search for SRs in 7 databases until April 16, 2022. Two reviewers independently extracted data and assessed the methodological quality of the reviews according to the Assessing the Methodological Quality of Systematic Reviews 2 (AMSTAR-2), the Risk of Bias in Systematic reviews (ROBIS) tool, and the Preferred Reporting Item for Systematic Review and Meta-analysis (PRISMA) statement. The Grading of Recommendations, Assessment, Development, and Evaluation (GRADE) was used to rate the quality of evidence.</p></div><div><h3>Results</h3><p>IBS-D patients included in 12 reviews were diagnosed in accordance with the Rome criteria, 9 reviews focused on CHM, 2 articles observed moxibustion and heat-sensitive moxibustion, 1 article studied acupuncture and CHM. The outcomes of the SRs were the effectiveness rate, the total effectiveness rate, global symptom improvement, and adverse effects. Based on AMSTAR-2, which measures the quality of methodology, all of the included studies were of low or critically low quality. According to the ROBIS tool, 10 SRs (83.33%) had a high risk of bias. With the PRISMA checklist, only 3 SRs reached over 90% compliance. Based on GRADE, most evidence was of low quality, and there was a moderate quality of evidence that the effectiveness rate of modified-Tongxie Yaofang was superior to Western medicine in the treatment of IBS-D.</p></div><div><h3>Conclusion</h3><p>Given the suboptimal reporting and methodological quality of existing SRs, more studies are needed to clarify whether TCM therapies are more effective or safe than pharmacological medicine. Future studies should combine evidence-based medicine with TCM research according to the characteristics of TCM.</p></div>","PeriodicalId":36624,"journal":{"name":"Journal of Traditional Chinese Medical Sciences","volume":"9 3","pages":"Pages 330-339"},"PeriodicalIF":0.0,"publicationDate":"2022-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2095754822000515/pdfft?md5=384257239d7115ecb57d40ad6b528b10&pid=1-s2.0-S2095754822000515-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47919632","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reporting guidelines of Chinese medicine: Current situation and future development","authors":"Xuan Zhang ,&nbsp;Wai Chak Andrew Chung ,&nbsp;Chung Tai Lau ,&nbsp;Nana Wang","doi":"10.1016/j.jtcms.2022.06.008","DOIUrl":"10.1016/j.jtcms.2022.06.008","url":null,"abstract":"<div><h3>Objective</h3><p>Reporting research is as important a part of a study as its design or analysis. Reporting guidelines (RGs) provide structured advice on how to report research studies clearly and adequately. This study aimed to review the development of RGs of Chinese medicine (CM) and to provide recommendations for improvement.</p></div><div><h3>Methods</h3><p>Through a systematic search of the Enhancing the QUAlity and Transparency Of health Research (EQUATOR) Network and electronic databases up to January 1, 2022, we identified a total of 15 RGs of CM, and further summarized their characteristics and applications. In addition, we reviewed the development of international RGs and analyzed its impact for CM.</p></div><div><h3>Results</h3><p>Compared with the generic RGs, the reporting standards of CM have been rapidly developed over the last 10 years, of which 57% were issued in recent 3 years (2019–2021). Currently, the system of RGs of CM has been established, especially for clinical trials, including various CM interventions and covering the guidelines from trial registration, protocol, results publication to the evidence synthesis and clinical practice guideline. However, the application of RGs of CM is suboptimal.</p></div><div><h3>Conclusion</h3><p>It is necessary to take further measures to promote practical application, improve journals’ endorsement, and establish quality monitoring procedures for RGs of CM in the future.</p></div>","PeriodicalId":36624,"journal":{"name":"Journal of Traditional Chinese Medical Sciences","volume":"9 3","pages":"Pages 209-216"},"PeriodicalIF":0.0,"publicationDate":"2022-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2095754822000564/pdfft?md5=e6a3d29b10417d603ad3d17c7f1dccfe&pid=1-s2.0-S2095754822000564-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44544090","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Loganin regulates glycolipid metabolism by influencing intestinal microbiota and AMPK signaling in obese mice","authors":"Bingrui Xu ,&nbsp;Zimengwei Ye ,&nbsp;Tian Tian ,&nbsp;Ruyuan Zhu ,&nbsp;Chenyue Liu ,&nbsp;Xin Fang ,&nbsp;Dongwei Zhang ,&nbsp;Min Fu ,&nbsp;Sihua Gao ,&nbsp;Dandan Zhao","doi":"10.1016/j.jtcms.2022.06.012","DOIUrl":"10.1016/j.jtcms.2022.06.012","url":null,"abstract":"<div><h3>Objective</h3><p>We aimed to observe the effects of loganin (Log) on serum glycolipid levels and probe the mechanisms focusing on intestinal flora and AMP-activated protein kinase (AMPK) signaling in obese mice.</p></div><div><h3>Methods</h3><p>A high-fat diet was given for 12 consecutive weeks to generate the obesity model in institute of cancer research (ICR) mice. Body weight was measured weekly and fasting blood glucose (FBG) was determined every 2 weeks. Both the oral glucose tolerance test and the intraperitoneal insulin tolerance test were performed. The serum levels of total cholesterol (TC), triglyceride, high-density lipoprotein-cholesterol, low-density lipoprotein-cholesterol (LDL-C), and free fatty acids (FFA) were measured. The expression of key proteins in the AMPK signaling pathway in skeletal muscle tissue was detected by immunoblotting, and gut microbiota were characterized using 16S rDNA sequencing.</p></div><div><h3>Results</h3><p>Log significantly decreased the body weight and the FBG in obese mice (<em>P</em> &lt; .05), and it could restore FBG to normal levels. The total cholesterol, LDL-C, and FFA levels were significantly reduced by Log compared with the obese controls (TC: <em>P</em> = .0020; LDL-C: <em>P</em> = .0233; FFA: <em>P</em> = .0127), and the glucose tolerance of animals was significantly improved (<em>P</em> = .0477). The western blot results showed that Log could upregulate the protein expression of Adenosine 5‘-monophosphate (AMP)-activated protein kinase (AMPKα), Sirtuin 1 (SIRT1), and peroxisome proliferator-activated receptor-gamma coactivator -1alpha (PGC1α) in skeletal muscle tissue of obese mice. 16S rDNA sequencing indicated that Log reduced the diversity of the gut flora in feces and altered the floral composition of obese mice.</p></div><div><h3>Conclusions</h3><p>Log was effective in reducing body weight and improving glucolipid metabolism in obese mice, probably through activating AMPK signaling and regulating intestinal microbial diversity.</p></div>","PeriodicalId":36624,"journal":{"name":"Journal of Traditional Chinese Medical Sciences","volume":"9 3","pages":"Pages 321-329"},"PeriodicalIF":0.0,"publicationDate":"2022-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2095754822000606/pdfft?md5=9bd0bfecdeecd84da984fa31c61885f4&pid=1-s2.0-S2095754822000606-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46036027","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of the clinical effect features of Han-Ku-Gan and Wen-Xin-Gan based on the efficacy of promoting blood circulation and removing blood stasis","authors":"Mengqi Huo ,&nbsp;Sha Peng ,&nbsp;Jing Li ,&nbsp;Yanfeng Cao ,&nbsp;Zhao Chen ,&nbsp;Yanling Zhang ,&nbsp;Yanjiang Qiao","doi":"10.1016/j.jtcms.2022.05.001","DOIUrl":"10.1016/j.jtcms.2022.05.001","url":null,"abstract":"<div><h3>Objective</h3><p>The application of Chinese materia medica (CMM) in clinical diseases is the embodiment and continuation of the property theory of CMM (PTCMM). However, due to a lack of precise quantitative description methods, it is difficult to systematically analyze the property of CMM (PCMM) and clinical effect features at the micro molecular level.</p></div><div><h3>Methods</h3><p>The therapeutic drugs and targets were obtained from the Drugbank database. The molecular descriptors of these drugs were calculated based on Dragon software. Drug–effect relationships that integrated the molecular descriptors and effect descriptors were plotted as grayscale images. These images were used to train the LeNet-5 model and the AlexNet model. The best-performing model was used to predict the effect features of the CMM compounds. Finally, the effect features of the PCMM combinations were calculated based on the support vector machine recursive feature elimination algorithm.</p></div><div><h3>Results</h3><p>The AlexNet model showed a superior prediction performance. The results showed that its accuracy, precision, sensitivity, F-measure, and Matthews correlation coefficient on the training set were 0.940, 0.936, 0.945, 0.940, and 0.880, respectively, and those of the test set were 0.909, 0.901, 0.920, 0.910, and 0.819, respectively. A total of 399 compounds in the 42 CMMs for promoting blood circulation and removing blood stasis were predicted by this model. The key effect features of the <em>Han</em>-<em>Ku</em>-<em>Gan</em> combination were anti-inflammatory, anti-tumor, anti-atherosclerosis, anti-Parkinson, hypoglycemic, and anti-coagulant properties, as well as excitation of uterine smooth muscle. The key effect features of the <em>Wen</em>-<em>Xin</em>-<em>Gan</em> combination were anti-inflammatory, anti-atherosclerosis, anti-hypertensive, anti-coagulant, anti-tumor, and anti-cardiac insufficiency effects, as well as enhanced immunity, sedation and hypnosis, and analgesia.</p></div><div><h3>Conclusion</h3><p>This study provides a new method for the further exploration of the relationship between the PCMM and clinical effect features.</p></div>","PeriodicalId":36624,"journal":{"name":"Journal of Traditional Chinese Medical Sciences","volume":"9 3","pages":"Pages 237-245"},"PeriodicalIF":0.0,"publicationDate":"2022-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2095754822000485/pdfft?md5=e00df1780ad8692f78bc30fcab26852d&pid=1-s2.0-S2095754822000485-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46190385","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Xiaoyu Jiangzhi capsule protects against heart failure via Ca2+/CaMKII signaling pathways in mice","authors":"Jianwei Wu ,&nbsp;Yafang Tan ,&nbsp;Dongyuan Kang ,&nbsp;Juan Yu ,&nbsp;Jianyong Qi ,&nbsp;Jiashin Wu ,&nbsp;Minzhou Zhang","doi":"10.1016/j.jtcms.2022.06.002","DOIUrl":"https://doi.org/10.1016/j.jtcms.2022.06.002","url":null,"abstract":"<div><h3>Objective</h3><p>Heart failure (HF), a worldwide health condition, is the result of many cardiovascular diseases. The traditional Chinese medicine (TCM) Xiaoyu Jiangzhi capsule (XYC) has long been in use in China to treat hyperlipidemia and inhibit platelet aggregation. This study explores the effects of XYC on heart failure (HF) and its detailed mechanisms.</p></div><div><h3>Methods</h3><p>Isoproterenol (ISO, 30 mg/kg) was injected intraperitoneally for 7 days to copy a HF model of 10–12 weeks old, 20–30 g male mice. We then compared the CON (control) group, ISO (HF model) group, MET (metoprolol) group, and XYC group. Cardiac systolic function and left wall thickness were evaluated by echocardiograph. Using western blot analysis, we detected the proteins of calmodulin dependent protein kinase II (CaMKII) and sarco/endoplasmic reticulum Ca²⁺-ATPase (Serca). Furthermore, tsA201 cells were cultured and the human CaV1.2 calcium channel current (hCaV1.2) were detected by patch clamp experiments.</p></div><div><h3>Results</h3><p>XYC reduced HF, inhibiting the protein expression of CaMKII, but Serca did not change significantly. Moreover, XYC inhibited the peak amplitude of the hCaV1.2 current, depolarizing shifted the activation curve 27.6 mV, and shifted the inactivation curve toward a positive potential 17.6 mV. The fraction recovered from inaction was reduced in XYC group compared with that in CON group.</p></div><div><h3>Conclusion</h3><p>XYC could inhibit ISO-induced HF by reducing the Ca²⁺/CaMKII signaling pathway in mice.</p></div>","PeriodicalId":36624,"journal":{"name":"Journal of Traditional Chinese Medical Sciences","volume":"9 3","pages":"Pages 289-297"},"PeriodicalIF":0.0,"publicationDate":"2022-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2095754822000503/pdfft?md5=5621e4c7652f907d19934dfc2397d18a&pid=1-s2.0-S2095754822000503-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72244002","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Qinzhi Zhudan formula improves memory and alleviates neuroinflammation in vascular dementia rats partly by inhibiting the TNFR1-mediated TNF pathway","authors":"Shuling Liu ,&nbsp;Fafeng Cheng ,&nbsp;Beida Ren ,&nbsp;Wenxiu Xu ,&nbsp;Congai Chen ,&nbsp;Chongyang Ma ,&nbsp;Xiaole Zhang ,&nbsp;Feifei Tang ,&nbsp;Qingguo Wang ,&nbsp;Xueqian Wang","doi":"10.1016/j.jtcms.2022.06.011","DOIUrl":"10.1016/j.jtcms.2022.06.011","url":null,"abstract":"<div><h3>Objective</h3><p>The Qinzhi Zhudan formula (QZZD) exhibits a prominent therapeutic effect in the treatment of vascular dementia (VaD). This study combined a network pharmacology approach and experimental validation to identify the underlying biological mechanism of QZZD against VaD.</p></div><div><h3>Methods</h3><p>Male Wistar rats received bilateral common carotid artery occlusion (BCCAO) surgery, and after 4 weeks of intragastric administration of QZZD, the therapeutic effect was assessed using the Morris water maze test and cerebral blood flow (CBF) assessment. Hematoxylin and eosin staining, Nissl staining, and electron microscopy were used to measure the histopathological changes in the neurons of rats. The effect of QZZD treatment on hippocampal neurotransmitters was assessed by high-performance liquid chromatography with electrochemical detection and liquid chromatography mass spectrometry. Immunofluorescence was used to observe VaD-induced microglia activation. The inflammatory cytokine levels were assessed by enzyme linked immunosorbent assay. Western blot was used to examine the TNFR1-mediated TNF pathway, which was screened out by network pharmacology analysis.</p></div><div><h3>Results</h3><p>QZZD treatment alleviated pathological changes and neuronal damage in VaD rats and attenuated their cognitive impairment. In addition, QZZD increased CBF and the expression of acetylcholine and 5-hydroxytryptamine in the hippocampal region. Notably, QZZD inhibited microglial activation and the expression of IL-6 and TNF-α. Network pharmacology and western blot indicated that QZZD inhibited the levels of TNFR1, NF-κBp65, p-ERK, TNF-α, and IL-6, which are related to the TNFR1-mediated TNF signaling pathway.</p></div><div><h3>Conclusion</h3><p>QZZD clearly improved learning and memory function, reduced brain pathological damage, elevated CBF and hippocampal neurotransmitter levels, and alleviated neuroinflammation of VaD rats partly by inhibiting the TNFR1-mediated TNF pathway, indicating its potential value in the clinical therapy of VaD.</p></div>","PeriodicalId":36624,"journal":{"name":"Journal of Traditional Chinese Medical Sciences","volume":"9 3","pages":"Pages 298-310"},"PeriodicalIF":0.0,"publicationDate":"2022-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S209575482200059X/pdfft?md5=f1346d900933d790d7dc1685aea63410&pid=1-s2.0-S209575482200059X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46459993","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Structural and functional characteristics of microbiota in oropharynx of sub-healthy children with gastrointestinal heat retention syndrome differentiated by traditional Chinese medicine","authors":"Jianhua Zhen ,&nbsp;He Yu ,&nbsp;Xiaofei Li ,&nbsp;Fei Dong ,&nbsp;Zi'an Zheng ,&nbsp;Xueyan Ma ,&nbsp;Yuxiang Wan ,&nbsp;Tiegang Liu ,&nbsp;Lu Fan ,&nbsp;Xiaohong Gu","doi":"10.1016/j.jtcms.2022.06.010","DOIUrl":"10.1016/j.jtcms.2022.06.010","url":null,"abstract":"<div><h3>Objective</h3><p>To explore the structural and functional characteristics of microbiota in oropharynx of sub-healthy children with gastrointestinal heat retention syndrome (GHRS) differentiated by traditional Chinese medicine, and screen the biometric operational taxonomic units (OTUs) to assist the clinical diagnosis.</p></div><div><h3>Methods</h3><p>We recruited children according to the “GHRS diagnostic scale”, collected their oropharyngeal swabs, and sequenced the 16S rDNA V4 region. We described the bacterial structure with alpha-indexes, beta-distances, and relative abundances; moreover, we screened the differential genera/OTUs with Wilcoxon rank-sum test, MetagenomeSeq analysis, and linear discriminant analysis effect size (LEfSe) analysis, in which biometric OTUs were selected to construct the receiver operating characteristic curve to verify the diagnostic value. The bacterial function was predicted with Kyoto Encyclopedia of Genes and Genomes pathways according to 16S rDNA gene by using Phylogenetic Investigation of Communities by Reconstruction of Unobserved States.</p></div><div><h3>Results</h3><p>The study population was composed of 10 children with GHRS and 10 healthy control children. GHRS children were more likely to overeat (gluttony, <em>P</em> = .033). Alpha-indexes, such as Sobs, abundance-based coverage estimator, Bootstrap, and Qstat, were significantly higher in the GHRS group, while beta-distances did not exhibit any significant intergroup differences. There were 9 differently distributed non-predominant genera between the groups in Wilcoxon rank-sum test, as well as 13 non-predominant genera in MetagenomeSeq analysis and 3 non-predominant OTUs in LEfSe analysis. OTU44 and OTU196 were used to construct the receiver operating characteristic curve, and the area under curve was 0.92. Predicted functions showed that pathways related to oxidative phosphorylation and carbon metabolism were enriched in healthy control samples, while the pathway related to renin secretion was remarkably enriched in GHRS samples.</p></div><div><h3>Conclusion</h3><p>Unique oropharyngeal microbial structure and function were identified in GHRS children. OTU44 and OTU196 were specific OTUs, which could be used as biomarkers of GHRS to assist clinical diagnosis.</p></div>","PeriodicalId":36624,"journal":{"name":"Journal of Traditional Chinese Medical Sciences","volume":"9 3","pages":"Pages 230-236"},"PeriodicalIF":0.0,"publicationDate":"2022-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2095754822000588/pdfft?md5=7f2c8980bbd76052102b05477348704e&pid=1-s2.0-S2095754822000588-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41992088","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fecal-associated microbiome differences between phlegm-dampness constitution and balanced constitution","authors":"Yini Li ,&nbsp;Pengfei Zhao ,&nbsp;Yunan Zhang ,&nbsp;Jianhua Zhen ,&nbsp;Lu Zhao ,&nbsp;Yanan Cai ,&nbsp;Qingyi Lu ,&nbsp;Guangrui Huang","doi":"10.1016/j.jtcms.2022.06.009","DOIUrl":"10.1016/j.jtcms.2022.06.009","url":null,"abstract":"<div><h3>Objective</h3><p>This study aimed to explore the structural and functional characteristics of the fecal-associated microbiome (FAM) between the phlegm-dampness constitution (PDC) and balanced constitution (BC), and to screen the related specific operational taxonomic unit (OTU) biomarkers.</p></div><div><h3>Methods</h3><p>This was a cross-sectional study. After strictly identifying the constitution of subjects, their clinical index was recorded and counted. Fecal samples were collected for 16S rDNA sequencing. Alpha diversity, beta diversity, and the relative abundance of dominant bacterial taxa were used to describe the FAM structure, and the Wilcoxon rank-sum test, MetagenomeSeq, and linear discriminant analysis effect size (LEfSe) were used to screen specific bacterial taxa. Specific OTUs were screened to construct receiver operating characteristic (ROC) curves.</p></div><div><h3>Results</h3><p>Thirty-two subjects were enrolled, including 22 subjects with BC and 10 subjects with PDC. There were significant differences in cold preference, levels of aspartate transaminase, β2-microglobulin, and creatine kinase MB, and alpha diversity indices (Shannon and Shannoneven) between the two groups. In principal coordinate analysis by abund-jaccard distance measure and partial least squares discriminant analysis, bacterial communities clustered separately between the two groups. Furthermore, based on MetagenomeSeq, LEfSe, and the Wilcoxon rank-sum test, a total of 43, 18, and 130 OTUs were differentially distributed between BC group and PDC group, respectively, and OTU200, OTU133, and OTU353 were screened when <em>P</em>≤ .01. The area under the ROC curve constructed from the 3 selected OTUs was 0.93.</p></div><div><h3>Conclusion</h3><p>The FAM structure and related functional characteristics of the PDC group differed from those of the BC group. In particular, OTU200, OTU133, and OTU353 can be used as unique markers of PDC to assist clinical diagnosis.</p></div>","PeriodicalId":36624,"journal":{"name":"Journal of Traditional Chinese Medical Sciences","volume":"9 3","pages":"Pages 257-266"},"PeriodicalIF":0.0,"publicationDate":"2022-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2095754822000576/pdfft?md5=7f11a5a1d10fc71a8eb9cea87ce23fdf&pid=1-s2.0-S2095754822000576-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42988832","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrated network pharmacology and experimental verification to explore the mechanism of Sangqi Qingxuan formula against hypertensive vascular remodeling","authors":"Lingling Li ,&nbsp;Jiayun Wu ,&nbsp;Ruiqi Yao ,&nbsp;Deshuang Yang ,&nbsp;Ying Chen ,&nbsp;Jin Zhang ,&nbsp;Li Huang","doi":"10.1016/j.jtcms.2022.06.007","DOIUrl":"https://doi.org/10.1016/j.jtcms.2022.06.007","url":null,"abstract":"<div><h3>Objective</h3><p>To investigate the bioactive components of Sangqi Qingxuan formula (SQQX), predict the pharmacological targets, and explore the mechanism of hypertensive vascular remodeling (HVR).</p></div><div><h3>Methods</h3><p>Network pharmacology was adopted to predict how SQQX acts in HVR. The effectiveness was assessed by blood pressure measurements and pathological morphology observation based on a spontaneously hypertensive rat model, while the mechanism of SQQX on HVR was validated by immunohistochemistry (IHC) and western blot (WB) according to the results of network pharmacology.</p></div><div><h3>Results</h3><p>There were 130 bioactive components of SQQX and 231 drug targets predicted by the Traditional Chinese Medicine Systems Pharmacology Database. Subsequently, 181 common targets were identified for SQQX against HVR, with TP53, MAPK1, and AKT1 as the core targets. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analyses was employed to identify the top 20 enriched functions and the top 20 pathways (<em>P</em> &lt; .01). Finally, the key role of the ERK/MAPK signaling pathway in HVR was determined. The <em>in vivo</em> results suggested that SQQX reduced systolic blood pressure and increased the ratio of thoracic aortic wall thickness to lumen diameter. Additionally, compared with the model group, SQQX increased the expression of smooth muscle 22 alpha (IHC: <em>P</em> &lt; .001; WB: <em>P</em> &lt; .05) and decreased the expression of osteopontin (IHC: <em>P</em> &lt; .001; WB: <em>P</em> &lt; .05), ERK1/2 (IHC: <em>P</em> &lt; .001; WB: ERK1 &amp; ERK2, all <em>P</em> &lt; .05), p-ERK1/2 (IHC: <em>P</em> &lt; .001; WB: ERK1 &amp; ERK2, all <em>P</em> &lt; .05), and the ratio of p-ERK1/2 to ERK1/2 protein (IHC: <em>P &lt;</em> .001).</p></div><div><h3>Conclusions</h3><p>SQQX, which has multiple bioactive ingredients and potential targets, is an effective treatment for HVR. The mechanism of antihypertensive and vascular protection may be related to the inhibition of phenotypic transformation of vascular smooth muscle cells and the ERK/MAPK signaling pathway.</p></div>","PeriodicalId":36624,"journal":{"name":"Journal of Traditional Chinese Medical Sciences","volume":"9 3","pages":"Pages 277-288"},"PeriodicalIF":0.0,"publicationDate":"2022-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2095754822000552/pdfft?md5=4d69ac438e89f34cabfb51eac8f4fad1&pid=1-s2.0-S2095754822000552-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72244003","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}