The Journal of pharmacy and pharmacology最新文献_第4页

Botany, traditional uses, phytochemistry, pharmacological and toxicological effects of Croton tiglium Linn.: a comprehensive review. 巴豆的植物学、传统用途、植物化学、药理和毒理学作用。全面的审查。

IF 3.3

The Journal of pharmacy and pharmacology Pub Date : 2022-08-19 DOI: 10.1093/jpp/rgac040

Ting Zhang, Zibo Liu, Xue Sun, Ziqi Liu, Lilin Zhang, Qing Zhang, Wei Peng, Chunjie Wu

{"title":"Botany, traditional uses, phytochemistry, pharmacological and toxicological effects of Croton tiglium Linn.: a comprehensive review.","authors":"Ting Zhang, Zibo Liu, Xue Sun, Ziqi Liu, Lilin Zhang, Qing Zhang, Wei Peng, Chunjie Wu","doi":"10.1093/jpp/rgac040","DOIUrl":"https://doi.org/10.1093/jpp/rgac040","url":null,"abstract":"Objectives: Croton tiglium Linn. (Euphorbiaceae) is an ancient medicinal plant that has been used for a long time, which is widely distributed in tropical and subtropical regions. And it is widely used for defecation, induced labour, treatment of gastrointestinal diseases, headache, as well as rheumatoid arthritis.Key findings: Approximately 150 compounds have been isolated and identified from the seeds, stems, leaves and branches of C. tiglium, including fatty acids, terpenoids, alkaloids, the plants proteins and other types of components. Based on a wide range of biological properties, C. tiglium has a wide range of pharmacological effects, such as antitumor, anti-HIV, analgesic, anti-inflammatory and antibacterial effects.Summary: The review aims to provide a critical and comprehensive evaluation of the botany, phytochemistry, pharmacology and toxicity of C. tiglium, with a vision for promoting further pharmaceutical research to explore its complete potential for better clinical application. The tigliane diterpenoids have been the most studied compounds isolated from C. tiglium, which showing a variety of biological activities, but there is insufficient evidence to explain the mechanism of action. In addition, C. tiglium may have potential toxic effects, and it is necessary to reduce the toxic effects to ensure the safety of clinical medication, which may promote the discovery and development of new drugs.","PeriodicalId":366080,"journal":{"name":"The Journal of pharmacy and pharmacology","volume":" ","pages":"1061-1084"},"PeriodicalIF":3.3,"publicationDate":"2022-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40071301","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Cell-penetrating peptide-mediated delivery of therapeutic peptides/proteins to manage the diseases involving oxidative stress, inflammatory response and apoptosis. 细胞穿透肽介导的治疗性肽/蛋白递送，以管理涉及氧化应激、炎症反应和细胞凋亡的疾病。

IF 3.3

The Journal of pharmacy and pharmacology Pub Date : 2022-08-19 DOI: 10.1093/jpp/rgac038

Issa Sadeghian, Reza Heidari, Mohammad Javad Raee, Manica Negahdaripour

{"title":"Cell-penetrating peptide-mediated delivery of therapeutic peptides/proteins to manage the diseases involving oxidative stress, inflammatory response and apoptosis.","authors":"Issa Sadeghian, Reza Heidari, Mohammad Javad Raee, Manica Negahdaripour","doi":"10.1093/jpp/rgac038","DOIUrl":"https://doi.org/10.1093/jpp/rgac038","url":null,"abstract":"Objectives: Peptides and proteins represent great potential for modulating various cellular processes including oxidative stress, inflammatory response, apoptosis and consequently the treatment of related diseases. However, their therapeutic effects are limited by their inability to cross cellular barriers. Cell-penetrating peptides (CPPs), which can transport cargoes into the cell, could resolve this issue, as would be discussed in this review.Key findings: CPPs have been successfully exploited in vitro and in vivo for peptide/protein delivery to treat a wide range of diseases involving oxidative stress, inflammatory processes and apoptosis. Their in vivo applications are still limited due to some fundamental issues of CPPs, including nonspecificity, proteolytic instability, potential toxicity and immunogenicity.Summary: Totally, CPPs could potentially help to manage the diseases involving oxidative stress, inflammatory response and apoptosis by delivering peptides/proteins that could selectively reach proper intracellular targets. More studies to overcome related CPP limitations and confirm the efficacy and safety of this strategy are needed before their clinical usage.","PeriodicalId":366080,"journal":{"name":"The Journal of pharmacy and pharmacology","volume":" ","pages":"1085-1116"},"PeriodicalIF":3.3,"publicationDate":"2022-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40150255","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Hyperoside, a natural flavonoid compound, attenuates Triptolide-induced testicular damage by activating the Keap1-Nrf2 and SIRT1-PGC1α signalling pathway. 金丝桃苷是一种天然类黄酮化合物，通过激活Keap1-Nrf2和SIRT1-PGC1α信号通路，减轻雷公藤甲素诱导的睾丸损伤。

IF 3.3

The Journal of pharmacy and pharmacology Pub Date : 2022-07-15 DOI: 10.1093/jpp/rgac011

Yucheng Wang, Jiaqi Li, Jingyu Gu, Wei He, Bo Ma, Hongqi Fan

{"title":"Hyperoside, a natural flavonoid compound, attenuates Triptolide-induced testicular damage by activating the Keap1-Nrf2 and SIRT1-PGC1α signalling pathway.","authors":"Yucheng Wang, Jiaqi Li, Jingyu Gu, Wei He, Bo Ma, Hongqi Fan","doi":"10.1093/jpp/rgac011","DOIUrl":"https://doi.org/10.1093/jpp/rgac011","url":null,"abstract":"Objectives: Hyperoside (Hyp), as the main ingredient from Semen Cuscutae, Abelmoschus moschatus, Acanthopanax senticosus, its protective effect in testicular dysfunction and mechanisms have not been studied. Here, we explored the action of Hyp in preventing oxidative stress-induced testicular damage and underlying mechanisms.Methods: The testicular injury model caused by oxidative stress was successfully built via Triptolide (TP) intraperitoneal injection in male mice. After Hyp (12.5, 25 and 50 mg/kg/day) treatment, testes weights, sperm count and morphology, histological changes, oxidative stress biomarkers from testicular tissue were detected. Also, the molecular mechanism was investigated by western blotting and immunohistochemistry assay.Key findings: These data suggested that Hyp significantly ameliorated TP-induced testicular atrophy, microstructural injury and spermatogenic dysfunction. Besides, it was shown that apoptosis-related proteins (cleaved caspase-3 and cleaved PARP) were prominently suppressed. The mechanical results indicated that Hyp significantly promoted Nrf2 translocation and elevated antioxidant enzymes expression in the testicular tissue. Meanwhile, this study also found that Hyp could improve TP-induced mitochondrial dysfunction via the SIRT1-PGC-1α signalling pathway.Conclusions: The present study indicated that Hyp exerted a potent ameliorative effect against testicular injury caused by oxidative stress via stimulating Keap1-Nrf2 and SIRT1-PGC1a signalling pathway.","PeriodicalId":366080,"journal":{"name":"The Journal of pharmacy and pharmacology","volume":" ","pages":"985-995"},"PeriodicalIF":3.3,"publicationDate":"2022-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40320033","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Correction to: Circ_UTRN ameliorates caerulein-induced acute pancreatitis in vitro via reducing inflammation and promoting apoptosis through miR-320-3p/PTK2 axis. 更正:Circ_UTRN通过miR-320-3p/PTK2轴减少炎症和促进细胞凋亡，改善体外小蛋白诱导的急性胰腺炎。

The Journal of pharmacy and pharmacology Pub Date : 2022-06-09 DOI: 10.1093/jpp/rgac025

Q. Sun, Ran Liang, Mingdong Li, Hua Zhou

引用次数: 0

Epidermal growth factor receptor as a potential target of momordin Ic to promote apoptosis of cholangiocarcinoma cells. 表皮生长因子受体作为苦瓜素Ic促进胆管癌细胞凋亡的潜在靶点。

The Journal of pharmacy and pharmacology Pub Date : 2022-05-30 DOI: 10.1093/jpp/rgac033

L. Senggunprai, V. Kukongviriyapan, A. Prawan, S. Kongpetch

{"title":"Epidermal growth factor receptor as a potential target of momordin Ic to promote apoptosis of cholangiocarcinoma cells.","authors":"L. Senggunprai, V. Kukongviriyapan, A. Prawan, S. Kongpetch","doi":"10.1093/jpp/rgac033","DOIUrl":"https://doi.org/10.1093/jpp/rgac033","url":null,"abstract":"OBJECTIVES\u0000Strategies that induce apoptosis of malignant cells are recognized as effective cancer treatments. This study evaluated the apoptosis-inducing ability of momordin Ic against cholangiocarcinoma (CCA) cells and the respective underlying mechanisms.\u0000\u0000\u0000METHODS\u0000Quantification of apoptotic cells was performed using Annexin V/7-AAD double dye staining followed by flow cytometry. The effect of momordin Ic on the expression of epidermal growth factor receptor (EGFR) and its downstream signalling molecules was determined via Western blot analysis. The RT2 Profiler PCR Array was used to determine the expression of cell death-associated genes. Expression levels of apoptosis-related proteins were examined using an apoptosis antibody array.\u0000\u0000\u0000KEY FINDINGS\u0000Momordin Ic potently limited the ability of CCA cells to thrive by promoting apoptotic cell death. This apoptosis-inducing activity was accompanied with suppression of expression of EGFR, p-EGFR, c-Myc and other downstream EGFR signalling-related molecules. Additional molecular analyses demonstrated that momordin Ic modified the expression profile of cell death-associated genes in CCA cells. Moreover, significant upregulation of apoptosis-activating proteins and downregulation of apoptosis-inhibiting protein were also observed after exposure to momordin Ic.\u0000\u0000\u0000CONCLUSIONS\u0000These results suggest that momordin Ic has a potential therapeutic opportunity for CCA treatment by acting as an EGFR suppressant.","PeriodicalId":366080,"journal":{"name":"The Journal of pharmacy and pharmacology","volume":"100 6 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123131646","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Circular RNA hsa_circ_0026344 suppresses gastric cancer cell proliferation, migration and invasion via the miR-590-5p/PDCD4 axis. 环状RNA hsa_circ_0026344通过miR-590-5p/PDCD4轴抑制胃癌细胞增殖、迁移和侵袭。

The Journal of pharmacy and pharmacology Pub Date : 2022-05-30 DOI: 10.1093/jpp/rgac032

Long Lv, Jinghu Du, Daorong Wang, Zeqiang Yan

{"title":"Circular RNA hsa_circ_0026344 suppresses gastric cancer cell proliferation, migration and invasion via the miR-590-5p/PDCD4 axis.","authors":"Long Lv, Jinghu Du, Daorong Wang, Zeqiang Yan","doi":"10.1093/jpp/rgac032","DOIUrl":"https://doi.org/10.1093/jpp/rgac032","url":null,"abstract":"OBJECTIVES\u0000Circular RNA (CircRNA) is a class of non-coding RNA transcripts, with multiple pathophysiological functions. Instead, the mechanism and function of circRNA in gastric cancer (GC) are not fully deciphered.\u0000\u0000\u0000METHODS\u0000CircRNA_0026344 (circ_0026344), microRNA (miR)-590-5p and programmed cell death 4 (PDCD4) mRNA expression levels in GC tissues and cells were probed by quantitative real-time PCR. Cell viability, migration and aggressiveness were examined by cell counting kit-8 and transwell assays. Additionally, the interplay among circ_0026344, miR-590-5p and PDCD4 was verified with bioinformatics and dual-luciferase reporter gene assay. Western blot was conducted to probe PDCD4 protein expression.\u0000\u0000\u0000KEY FINDINGS\u0000Circ_0026344 expression was underexpressed in GC tissues and cells, which was associated with clinicopathological characteristics such as tumour size, tumor-node-metastasis stage and lymph node metastasis. Circ_0026344 overexpression restrained the malignant biological behaviours of GC cells, while circ_0026344 knockdown functioned oppositely. Circ_0026344 could act as a competing endogenous RNA of miR-590-5p to negatively modulate its expression, and this miRNA could mitigate the impact of circ_0026344 on GC cells. In addition, PDCD4 was identified as the downstream target of miR-590-5p, and PDCD4 expression was positively modulated by circ_0026344.\u0000\u0000\u0000CONCLUSIONS\u0000Circ_0026344 up-regulates PDCD4 expression via sponging miR-590-5p, thus inhibiting the progression of GC. This study further expounds the underlying molecular mechanism in the GC progression.","PeriodicalId":366080,"journal":{"name":"The Journal of pharmacy and pharmacology","volume":"16 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127277183","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Lianhuaqingwen alleviates p53-mediated apoptosis in alveolar epithelial cells to prevent LPS-induced ALI. 连花清文可减轻p53介导的肺泡上皮细胞凋亡，预防lps诱导的ALI。

The Journal of pharmacy and pharmacology Pub Date : 2022-05-30 DOI: 10.1093/jpp/rgac035

Ruhao Yang, Haizhen Yang, Wenqiang Li, F. Yue, Hao Chen, Yueying Hao, Ke Hu

{"title":"Lianhuaqingwen alleviates p53-mediated apoptosis in alveolar epithelial cells to prevent LPS-induced ALI.","authors":"Ruhao Yang, Haizhen Yang, Wenqiang Li, F. Yue, Hao Chen, Yueying Hao, Ke Hu","doi":"10.1093/jpp/rgac035","DOIUrl":"https://doi.org/10.1093/jpp/rgac035","url":null,"abstract":"BACKGROUND\u0000Our previous study found that Lianhuaqingwen reduces lipopolysaccharide (LPS)-induced acute lung injury (ALI) in mice by suppressing p53-mediated apoptosis. To identify the type of lung cells affected by Lianhuaqingwen, we conducted a cell experiment.\u0000\u0000\u0000METHODS\u0000C57/B6 mice and A549 cells were administered Lianhuaqingwen and LPS. A549 cells were transfected with p53 siRNA to inhibit p53. The degree of ALI in mice was validated by haematoxylin and eosin staining as well as measurement of IL-1β and MCP-1 levels. In A549 cells, Cell Counting Kit-8 (CCK-8), DHE and TUNEL assays were used to assess cell viability, reactive oxygen species (ROS) production and apoptosis, respectively. Western blot analysis was used to evaluate the protein expression of p53, Bcl-2, Bax, caspase-9 and caspase-3. Co-immunofluorescence was used to detect cytochrome C distribution.\u0000\u0000\u0000KEY FINDINGS\u0000Lianhuaqingwen alleviated LPS-induced ALI in vivo. Lianhuaqingwen at 300 μg/ml increased cell viability, lowered ROS production and reduced apoptotic cells in vitro. Lianhuaqingwen enhanced Bcl-2 expression and reduced Bax, caspase-9 and caspase-3 expression as well as blocked cytochrome C release under LPS stimulation. Treatment with a combination of Lianhuaqingwen and p53 siRNA was more effective than treatment with Lianhuaqingwen alone.\u0000\u0000\u0000CONCLUSION\u0000Lianhuaqingwen inhibits p53-mediated apoptosis in alveolar epithelial cells, thereby preventing LPS-induced ALI.","PeriodicalId":366080,"journal":{"name":"The Journal of pharmacy and pharmacology","volume":"2017 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122217124","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Development of a comprehensive method based on quantitative 1 H NMR for quality evaluation of Traditional Chinese Medicine injection: a case study of Danshen Injection. 基于核磁共振定量评价中药注射剂质量的综合方法建立——以丹参注射液为例。

The Journal of pharmacy and pharmacology Pub Date : 2022-05-27 DOI: 10.1093/jpp/rgac034

Wenzhu Li, Fang Zhao, Jiayu Yang, Jianyang Pan, H. Qu

{"title":"Development of a comprehensive method based on quantitative 1 H NMR for quality evaluation of Traditional Chinese Medicine injection: a case study of Danshen Injection.","authors":"Wenzhu Li, Fang Zhao, Jiayu Yang, Jianyang Pan, H. Qu","doi":"10.1093/jpp/rgac034","DOIUrl":"https://doi.org/10.1093/jpp/rgac034","url":null,"abstract":"OBJECTIVES\u0000This study aimed to establish a rapid and comprehensive method for quantitative determination of complex ingredients in Traditional Chinese Medicine injections.\u0000\u0000\u0000METHODS\u0000A 1H quantitative nuclear magnetic resonance method was developed to simultaneously quantify comprehensive chemical components in Danshen Injection. Multivariate statistical analysis technique was applied to quality evaluation of multiple batches of Danshen injection.\u0000\u0000\u0000KEY FINDINGS\u0000A complete signal attribution to the 1H nuclear magnetic resonance spectrum of Danshen injection was developed and performed for the first time. A total of 32 chemical components were identified from Danshen Injection. Among them, 20 were quantified simultaneously, accounting for up to 80% (w/w) of the total solids and 95% (w/w) of total organic matter, representing success compared to the previous studies. The developed method was further applied to analyze 13 batches of Danshen Injection from three manufacturers to make a realistic analysis.\u0000\u0000\u0000CONCLUSION\u0000It was found that the comprehensive chemical information provides an adequate characterization for quality profiles among different commercial batches of Danshen Injection. The developed method further offered a guarantee for improving the consistency and safety of Traditional Chinese Medicine injections.","PeriodicalId":366080,"journal":{"name":"The Journal of pharmacy and pharmacology","volume":"46 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134060863","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Effect of carrier material on the thermodynamic properties and recrystallization kinetics of andrographolide-solid dispersion. 载体材料对穿心莲内酯-固体分散体系热力学性质和再结晶动力学的影响。

IF 3.3

The Journal of pharmacy and pharmacology Pub Date : 2022-05-20 DOI: 10.1093/jpp/rgab179

Guowei Zhao, Junfang Zhang, Ping Cai, Liquan Ou, Xinli Liang, Wei Dong, Zhenggen Liao

{"title":"Effect of carrier material on the thermodynamic properties and recrystallization kinetics of andrographolide-solid dispersion.","authors":"Guowei Zhao, Junfang Zhang, Ping Cai, Liquan Ou, Xinli Liang, Wei Dong, Zhenggen Liao","doi":"10.1093/jpp/rgab179","DOIUrl":"https://doi.org/10.1093/jpp/rgab179","url":null,"abstract":"Objectives: This work investigated the effect of the lipophilic long carbon chain of carrier material on the thermodynamic properties and the recrystallization kinetics of solid dispersion (SD).Methods: The thermodynamic properties and recrystallization kinetic parameters of amorphous andrographolide (AG)-PEG8000 laurate (SM12)-SD, AG-PEG8000 palmitate (SM16)-SD and AG-PEG8000 behenate (SM22)-SD were determined and calculated by differential scanning calorimetry combining AGV equation and Avrami equation.Key findings: From AG-SM12-SD to AG-SM22-SD with the increase of the carbon chain length of carrier material, the glass transition temperature, the maximum relaxation enthalpy and the mean relaxation time of SD increased at first and then decreased; the isothermal crystallization rate constants at different temperatures and non-isothermal crystallization rate constants at different heating rates of SD showed a trend of decreasing at first and then increasing.Conclusions: Increasing the carbon chain length of polyethylene glycol fatty acid ester can improve the space-limiting effect of the carrier material on the AG molecule, but the carbon chain length of carrier was not the longer the better. SM16 had the appropriate spatial scale, which better limited the molecular mobility of AG in SD, so AG-SM16-SD has better thermodynamic stability and recrystallization kinetic stability.","PeriodicalId":366080,"journal":{"name":"The Journal of pharmacy and pharmacology","volume":" ","pages":"761-768"},"PeriodicalIF":3.3,"publicationDate":"2022-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39607893","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pharmacological investigation of antioxidant and anti-inflammatory activities of aqueous extract from Mitracarpus frigidus (Rubiaceae). 水提物抗氧化和抗炎活性的药理研究。

IF 3.3

The Journal of pharmacy and pharmacology Pub Date : 2022-05-20 DOI: 10.1093/jpp/rgac005

Ari Sérgio de O Lemos, Lara M Campos, Thalita de F Souza, Priscila de L Paula, Juliana da T Granato, João Victor G da Silva, Danielle M O Aragão, Vinicius N Rocha, Elaine S Coimbra, Rodrigo L Fabri

{"title":"Pharmacological investigation of antioxidant and anti-inflammatory activities of aqueous extract from Mitracarpus frigidus (Rubiaceae).","authors":"Ari Sérgio de O Lemos, Lara M Campos, Thalita de F Souza, Priscila de L Paula, Juliana da T Granato, João Victor G da Silva, Danielle M O Aragão, Vinicius N Rocha, Elaine S Coimbra, Rodrigo L Fabri","doi":"10.1093/jpp/rgac005","DOIUrl":"https://doi.org/10.1093/jpp/rgac005","url":null,"abstract":"Objectives: This study aimed to evaluate the potential of aqueous extract from Mitracarpus frigidus aerial parts (MFAq) in the treatment of inflammation and oxidative stress, as well as to characterize its chemical constituents.Methods: Total phenolic and flavonoid contents were determined, and phytoconstituents were detected by ultra-fast liquid chromatography/quadrupole-time-of-flight tandem mass spectrometry (UFLC-QTOF-MS). The antioxidant activity was evaluated by DPPH, TAC and β-carotene/linoleic acid assays. In-vitro anti-inflammatory activity, cell viability and cell cycle were performed in J774A.1 cell line. In-vivo anti-inflammatory activity was investigated by two ear oedema assays (croton oil and phenol).Key findings: Chlorogenic acid, clarinoside, quercetin-hexosylpentoside, rutin, kaempferol-3-O-rutinoside, kaempferol-rhamnosylhexoside, quercetin-pentosylrhamnosylhexoside, harounoside, 2-azaanthraquinone and sucrose were identified by UFLC-QTOF-MS. MFAq showed antioxidant activity, which was positively correlated to the content of phenolic compounds. MFAq significantly inhibited the production of nitric oxide, did not decrease viability in MTT assay (all concentrations) and showed no changes in membrane permeability and cell cycle of J774A.1 cell line. Furthermore, MFAq showed a reduction in ear oedema in all tested doses.Conclusion: MFAq was effective in some antioxidant and inflammatory parameters, in the experimental conditions that were used in the study. This is the first report of chemical composition and bioactivities from this extract.","PeriodicalId":366080,"journal":{"name":"The Journal of pharmacy and pharmacology","volume":" ","pages":"750-760"},"PeriodicalIF":3.3,"publicationDate":"2022-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40320036","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1