Genetics of Aquatic Organisms最新文献_第3页

Liver Transcriptome and Molecular Responses of Giant Gourami (Osphronemus goramy) Fed with Different Dietary Carbohydrate Levels 饲粮不同碳水化合物水平对巨Gourami肝脏转录组和分子反应的影响

Genetics of Aquatic Organisms Pub Date : 2022-03-11 DOI: 10.4194/ga494

D. N. Sari, J. Ekasari, H. Nasrullah, M. Suprayudi, A. Alimuddin

{"title":"Liver Transcriptome and Molecular Responses of Giant Gourami (Osphronemus goramy) Fed with Different Dietary Carbohydrate Levels","authors":"D. N. Sari, J. Ekasari, H. Nasrullah, M. Suprayudi, A. Alimuddin","doi":"10.4194/ga494","DOIUrl":"https://doi.org/10.4194/ga494","url":null,"abstract":"Giant gourami (Osphronemus goramy) has been known to have a high ability to utilize high dietary carbohydrates. However, its molecular information related to carbohydrate metabolism is still limited. The present study elucidated Giant gourami's liver transcriptome and molecular responses to high dietary carbohydrates. Two semi-purified diets with different levels of dietary carbohydrate, normal carbohydrate (34%, N34) and high carbohydrate (53%, H53), were offered to the fish for 60 days. A total of 87,430,553 clean reads were collected and assembled into 49,034 cDNA contigs. Annotation was performed, and a total of 33,104 unigenes were obtained. High dietary carbohydrates generally down-regulated the differentially expressed genes (DEGs). The genes expression related to glycolysis and the antioxidant system were significantly upregulated (P<0.05). Meanwhile, the genes expression related to insulin signaling and gluconeogenesis were significantly downregulated (P>0.05). The results showed that Giant gourami has several adaptabilities for high carbohydrates. The upregulation of the glycolytic, gpx, and noa1 genes, downregulation of insulin signaling, and gluconeogenic genes indicated a good glucose homeostasis maintenance in Giant gourami.","PeriodicalId":36569,"journal":{"name":"Genetics of Aquatic Organisms","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45783287","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Functional Annotation of Uncharacterized Protein from Photobacterium damselae subsp. piscicida (Pasteurella piscicida) and Comparison of Drug Target Between Conventional Medicine and Phytochemical Compound Against Disease Treatment in Fish: An In-silico Approach 光杆菌damselae亚种未鉴定蛋白的功能注释piscicida (piscicida)和传统药物与植物化学化合物治疗鱼类疾病的药物靶点比较:一种计算机方法

Genetics of Aquatic Organisms Pub Date : 2022-01-18 DOI: 10.4194/ga453

Sk Injamamul Islam, M. Jahan

{"title":"Functional Annotation of Uncharacterized Protein from Photobacterium damselae subsp. piscicida (Pasteurella piscicida) and Comparison of Drug Target Between Conventional Medicine and Phytochemical Compound Against Disease Treatment in Fish: An In-silico Approach","authors":"Sk Injamamul Islam, M. Jahan","doi":"10.4194/ga453","DOIUrl":"https://doi.org/10.4194/ga453","url":null,"abstract":"Photobacterium damselae is one of the deadliest bacterial pathogens in the world of aquaculture. P. damselae has an uncharacterized protein (UniprotKb ID: A0PB24) that can enter the host cell and induce infection in fish, according to research. Domains of unknown function are proteins that have been identified experimentally but do not have a known functional or structural domain. Targeting this uncharacterized protein, we found that this protein may have link with other virulent proteins of P. damselae. So, the main objective of the study was to reveal the functional annotation of the protein and compare the inhibitory potentiality of conventional drug (Flumequine, Oxytetracycline and Florfenicol) and four potential antibacterial compounds from Syzygium aromaticum which reported previously. The uncharacterized protein was modeled, refined, and validated in this experiment. The protein-protein interaction network suggested that this uncharacterized protein may serve as a DNA/RNA hydrase and conserved domain search predict two different domains. Furthermore, the binding affinity of conventional drugs and phytochemical compounds were calculated against the protein with the docking approaches and predict S. aromaticum inhibitory compounds bind strongly with the protein. In addition, the ADMET properties of the compounds confirmed the drug likeness properties of the four compounds.","PeriodicalId":36569,"journal":{"name":"Genetics of Aquatic Organisms","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46496552","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

The Mitochondrial Genome of the Freshwater Crab Potamon fluviatile, the First Sequenced Representative of the Subfamily Potaminae and Its Phylogenetic Position within Potamidae 马铃薯亚科第一序列代表河蟹的线粒体基因组及其在马铃薯亚科中的系统发育地位

Genetics of Aquatic Organisms Pub Date : 2022-01-04 DOI: 10.4194/ga460

A. Vella, N. Vella

引用次数: 1

Identification and Molecular Investigation of Diplostomum in Capoeta umbla Caught from Freshwater Sources, Turkey 从土耳其淡水资源中捕获的圆腹蛛双口虫的鉴定和分子研究

Genetics of Aquatic Organisms Pub Date : 2021-12-20 DOI: 10.4194/ga454

Sibel Doğan Barata, M. Dörücü, Murad Gürses

引用次数: 0

Modulation of Muscle Growth Related Genes in Fish in Response to Plant Based Ingredients 鱼类肌肉生长相关基因对植物成分的调节

Genetics of Aquatic Organisms Pub Date : 2021-12-06 DOI: 10.4194/ga413

C. Ayisi, Gyamfua Afriyie, Gilbert Owusu-Afriyie

引用次数: 0

First Record of a Novel Mitochondrial DNA Haplotype of a Marble Trout Lineage of Salmo Trutta in Turkey 土耳其Salmo Trutta大理鳟鱼系新线粒体DNA单倍型的首次记录

Genetics of Aquatic Organisms Pub Date : 2021-08-26 DOI: 10.4194/ga452

F. Bardakci, Nazan Acar, T. Arslan, R. Badraoui

引用次数: 0

Assessment of Responses of Common Carp, Cyprinus carpio, Exposed to Sub-Lethal Concentrations of Lead, by Molecular and Mucosal Biomarkers 用分子和粘膜生物标志物评价鲤鱼对亚致死浓度铅的反应

Genetics of Aquatic Organisms Pub Date : 2021-07-09 DOI: 10.4194/ga448

Fakhriyeh Omidi, H. Jafaryan, R. Patimar, M. Harsij, H. Paknejad

{"title":"Assessment of Responses of Common Carp, Cyprinus carpio, Exposed to Sub-Lethal Concentrations of Lead, by Molecular and Mucosal Biomarkers","authors":"Fakhriyeh Omidi, H. Jafaryan, R. Patimar, M. Harsij, H. Paknejad","doi":"10.4194/ga448","DOIUrl":"https://doi.org/10.4194/ga448","url":null,"abstract":"Heavy metals are one of the most persistent pollutants in waters, and molecular biomarkers as bioindicators could uncover early warning signals of stress suffered by organisms exposed to these pollutants. This research was conducted to explore the impact of lead contaminant on expression levels of hsp70 gene as well as protein pattern and innate immune indicators of skin mucus in Common carp as a biological model. The fish were exposed to different concentrations of Pb(NO3)2 as a source of lead (0, 7.875, 15.75, and 31.5 mg/L of lead) for 14 days. After the completion of the experiment, the liver and gill tissue plus mucus samples were collected from the fish. Next, the effects of exposure to sublethal doses of lead were investigated on expression levels of hsp70 gene, protein pattern, and innate immune indicators of skin mucus. The results indicated that the expression level of hsp70 gene significantly increased (at 7.5% & 30% LC50) in the liver tissue compared to the control group (P<0.05). The protein pattern of treatment samples was different from that of the control group. Also, in the mucus innate immune parameters, the levels of alkaline phosphatase increased significantly at high concentrations of lead contamination in comparison with the control group (P<0.05). The protein levels first revealed an ascending trend (P<0.05), and then a descending trend (at 30% LC50), though it was not a significant downward trend compared with the control group (P>0.05). The results suggested that these physiological indicators in Cyprinus carpio could be used as molecular and mucosal biomarkers for ecotoxicological studies.","PeriodicalId":36569,"journal":{"name":"Genetics of Aquatic Organisms","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46603519","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Cost-effective DNA Isolation Strategy from Crustaceans Enables the First Molecular Phylogenetic Identification of Moina macrocopa from India 一种从甲壳类动物中分离DNA的低成本策略使来自印度的Moina macrocopa首次分子系统发育鉴定成为可能

Genetics of Aquatic Organisms Pub Date : 2021-06-18 DOI: 10.4194/2459-1831-v5_2_04

Shital Bhanushali, Kritika Katti, J. Ramchandani, S. Sen

{"title":"A Cost-effective DNA Isolation Strategy from Crustaceans Enables the First Molecular Phylogenetic Identification of Moina macrocopa from India","authors":"Shital Bhanushali, Kritika Katti, J. Ramchandani, S. Sen","doi":"10.4194/2459-1831-v5_2_04","DOIUrl":"https://doi.org/10.4194/2459-1831-v5_2_04","url":null,"abstract":"We report the first barcode identification of water flea species Moina macrocopa (strain JSK1) from the Indian subcontinent. The strain isolated from a freshwater pond near Mumbai by collective efforts of CUBE (Collaborative Undergraduate Biology Education), was morphologically characterized as Moina species. Keeping up with the growing demand of affordable science in colleges and to avoid hazardous technologies, we established a home grown porcelain-abrasion DNA extraction strategy for crustaceans, and compared it against routinely used protocols employing liquid nitrogen or insect-DNA isolation kits. Our method successfully yielded high quality genomic DNA from Moina, comparable to published protocols as further analysed by restriction digestion, ligation and PCR efficiency. We also sequenced two barcoding loci namely, COI and ITS sequences, duly submitted and curated by Genbank (accession numbers MH734122.1 and MH745035.1). Molecular phylogenetic analyses identified the Indian species as Moina macrocopa and related it closely to the Russian clade. Protein prediction analyses of the variations in COI revealed that the missense mutations lie in the aliphatic membrane helices and should not affect the catalytic capacity of cytochrome oxidase. The strain JSK1 is currently disseminated and maintained collectively by CUBE students across India, towards developing it as a model system.","PeriodicalId":36569,"journal":{"name":"Genetics of Aquatic Organisms","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-06-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47760267","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Clinical Investigation and Molecular Diagnosis of Microsporidian Enterocytozoon hepatopenaei (EHP) in Shrimps from Selangor, Malaysia 马来西亚雪兰莪虾肝原肠细胞微孢子虫(EHP)的临床研究及分子诊断

Genetics of Aquatic Organisms Pub Date : 2021-06-02 DOI: 10.4194/2459-1831-v5_2_03

S. Marimuthu, C. S. Raju, S. Bhassu

{"title":"Clinical Investigation and Molecular Diagnosis of Microsporidian Enterocytozoon hepatopenaei (EHP) in Shrimps from Selangor, Malaysia","authors":"S. Marimuthu, C. S. Raju, S. Bhassu","doi":"10.4194/2459-1831-v5_2_03","DOIUrl":"https://doi.org/10.4194/2459-1831-v5_2_03","url":null,"abstract":"In recent years, shrimp aquaculture production in Southeast Asia countries was highly infected by, microsporidian parasite, EHP. Recently, shrimps from farms located in Selangor were encountering growth retardation and it highly concerned us to carry out a clinical investigation in both farms (Location A and B). EHP infected P. monodon samples were collected from both locations. A total of 43 shrimp samples were collected and diagnosed via PCR using the 18S rRNA gene. Environmental parameters were found relatively higher than the advised values. It indicates that the farms are highly infected and toxic which had led to growth retardation and mortality. However, no significant differences were observed (except water temperatures) between the locations. The phylogenetic analysis expressed EHP detected from Malaysia formed in one clade and all the reference sequences clustered based on geographical continents (Asian-Latin America). Minimum-spanning network analysis shows that Malaysia samples Location A are genetically related with other Asian EHP samples even though the total positive rate of EHP infection was lower. However, samples from Location B were not clustered within the EHP clade and shows the highest genetic variance within Malaysia and among Asian samples. This preliminary investigation results confirmed the incidence of EHP outbreak in Malaysia.","PeriodicalId":36569,"journal":{"name":"Genetics of Aquatic Organisms","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42661497","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

In Silico Prediction of Molecular Interaction Within PmCBP-VP24 Complex to Understand Initial Instigation of WSSV into Shrimps PmCBP-VP24复合物内分子相互作用的原位硅预测，以了解WSSV最初向虾体内的诱导作用

Genetics of Aquatic Organisms Pub Date : 2021-05-13 DOI: 10.4194/2459-1831-v5_2_05

Kanika Yadav, A. K. Verma, A. Pathak, Abhishek Awasthi

引用次数: 3