{"title":"Genetic Variation Study within (Ompok bimaculatus and Clupisoma sinensis) Populations in Samarra Dam Middle of Iraq and Shatt Al-Arab Southern of Iraq belonging to Tigris River Using Mitochondrial Cytochrome b Gene","authors":"Rabeeha Mankhi Jebur","doi":"10.4194/2459-1831-v3_2_03","DOIUrl":"https://doi.org/10.4194/2459-1831-v3_2_03","url":null,"abstract":"This study was specifically aimed at Genetic Variation Study Within (Ompok bimaculatus & Clupisoma sinensis) Populations, and evaluated partial Cytb gene sequence of mtDNA for determining the genetic variation within Ompok bimaculatus & Clupisoma sinensis populations in Samarra Dam Middle of Iraq and Shatt Al-Arab Southern of Iraq belonging to Tigris River Using Mitochondrial Cytochrome b Gene. DNA extracted from 80 specimens of the two species of interest from two stations were analyzed. The Size of gene was (amplified Cytb gene) 1,118 bp long and 1,138bp long for O. bimaculatus and C. sinensis, respectively. By using Software DnaSP v5.1, the study revealed that there are two individual species in Samarra Dam and 3 in Shatt-AlArab river whereas 2 haplotypes in C. sinensis population in Shatt Al-Arab and only one hap. In Samarra Dam, there is also Sequencing of Cytb fragments. According to the molecular variance analysis, analyzes of nucleotide diversity and haplotypes pattern were performed separately for each community using the DnaSP v5.10 program. The analysis revealed that there are two types of specific haplotypes in (O. bimaculatus) in the Samarra Dam and 3 of the haplotypes patterns observed in the Shatt al-Arab River, while there are two haplotypes patterns in (St. Sinensis) in the Shatt al-Arab and 1 of haplotypes in Samarra Dam. Low range values of nucleotide and haplotype diversity for both species in Shatt Al-Arab river populations were revealed which refer to low genetic variation within each population in Shatt Al-Arab. As well as low range values of π and Hd for (O. bimaculatus) species in Samarra Dam populations which revealed low genetic variation while no genetic variation in (C. sinensis) population.","PeriodicalId":36569,"journal":{"name":"Genetics of Aquatic Organisms","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42471754","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"A Magnetic Bead-Based DNA Extraction Protocol Suitable for High-Throughput Genotyping in Shrimp Breeding Programs","authors":"Cheryl K. Y. Tan, J. Cowley, D. Jerry","doi":"10.4194/2459-1831-v3_2_02","DOIUrl":"https://doi.org/10.4194/2459-1831-v3_2_02","url":null,"abstract":"Due to their convenience, magnetic bead-based nucleic acid extraction kits are commonly used in shrimp genotyping and pathogen screening applications. However, in advanced breeding programs requiring the testing of many thousands of shrimp, their cost can be prohibitive. Various permutations of different Proteinase K digestion, tissue lysis and bead washing buffers as well as magnetic bead types were thus evaluated to devise a high-throughput shrimp DNA extraction (SDE) protocol capable of recovering high-purity DNA using a KingFisher™ Flex Magnetic Particle processor. When genotyped using a MassARRAY® platform (Agena Bioscience) requiring 60-61 genome regions to be co-amplified in a single multiplexed PCR, DNA extracted from shrimp muscle tissue using either the SDE protocol or a commercial kit generated comparable single-nucleotide polymorphism (SNP) call data. The SDE protocol also extracted high-purity DNA from salmon fin clips. It thus offers potential to markedly reduce the costs of large-scale genotyping in shrimp and salmon breeding programs.","PeriodicalId":36569,"journal":{"name":"Genetics of Aquatic Organisms","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2019-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42409594","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}