Journal of Advances in Medical and Biomedical Research最新文献_第7页

Multidrug-Resistant Strains of Acinetobacter baumannii from Intensive Care Unit Patients Show Genetic Diversity and Distribution of Genes Associated with Aminoglycoside Resistance 重症监护病房患者鲍曼不动杆菌多重耐药菌株显示与氨基糖苷耐药相关基因的遗传多样性和分布

Journal of Advances in Medical and Biomedical Research Pub Date : 2022-10-01 DOI: 10.30699/jambs.30.143.544

Leili Osanloo, H. Zeighami, F. Haghi, R. Shapouri, Rasoul Shokri

引用次数: 0

Pseudo Cor Triatriatum Sinistrum due to Dilated Coronary Sinus in Association with Supramitral Ring 冠状窦扩张伴二尖瓣上环引起的假性三尖窦性甲状腺肿

Journal of Advances in Medical and Biomedical Research Pub Date : 2022-10-01 DOI: 10.30699/jambs.30.143.570

Z. Khajali, Nasibeh Mohammadi, Raheleh Kaviani, H. Pouraliakbar, N. Parsa

引用次数: 0

Mesenchymal Stem Cells Trigger Epithelial to Mesenchymal Transition in the HT-29 Colorectal Cancer Cell Line 间充质干细胞触发HT-29结直肠癌癌症细胞系的上皮-间充质转变

Journal of Advances in Medical and Biomedical Research Pub Date : 2022-10-01 DOI: 10.30699/jambs.30.143.477

Sepideh Mirzaei, Kiavash Hushmandi, M. Entezari, A. Bahonar, M. Raei, M. Akbari

{"title":"Mesenchymal Stem Cells Trigger Epithelial to Mesenchymal Transition in the HT-29 Colorectal Cancer Cell Line","authors":"Sepideh Mirzaei, Kiavash Hushmandi, M. Entezari, A. Bahonar, M. Raei, M. Akbari","doi":"10.30699/jambs.30.143.477","DOIUrl":"https://doi.org/10.30699/jambs.30.143.477","url":null,"abstract":"10.30699/jambs.30.143.477 Background & Objective: Mesenchymal stem cells (MSCs) promote metastasis in colorectal cancer; however, the mechanism underlying this process is not fully understood. Epithelial to mesenchymal transition (EMT) is a key step in tumor acquisition of metastatic phenotype. We aimed to investigate the effect of MSCs on the expression of EMT markers, as well as cancer stem cell markers in HT-29 colorectal cancer cells. Materials & Methods: MSCs were isolated from bone marrow tissue, and their multi potency was confirmed. The HT-29 cell line was prepared and co-cultured with MSCs for 3 days using 6-well transwell co-culture plates (membrane pore size: 0.4 µm). Cell morphology was observed by inverted microscopy. The expression levels of EMT-related genes, namely E- cadherin, Vimentin, and β -catenin, were investigated by the RT-qPCR method. Also, the surface expression levels of CD44 and CD133 cancer stem cell markers were analyzed by flow cytometry. Results: The co-culture of HT-29 cells with bone marrow-derived MSCs resulted in changes in cell morphology from epithelial to mesenchymal forms. The expression of mesenchymal stem cell markers, namely Vimentin and β -catenin, were significantly increased (2.25 and 1.83 folds, respectively), while the expression of the epithelial marker, E-cadherin, was reduced (0.3 folds). The expression of CD133 was also increased (51.5%). Conclusion: Tumor-resident mesenchymal stem cells can promote colorectal cancer metastasis inducing EMT as well as increasing cancer stem cell frequency in the tumor microenvironment. It seems that direct contact between MSCs and colorectal cancer cells is not required for the interaction. Our findings may help scientists to find effective strategies against cancer metastasis by targeting tumor-resident MSCs.","PeriodicalId":36550,"journal":{"name":"Journal of Advances in Medical and Biomedical Research","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46489747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Investigating the Therapeutic Effect of β-Interferon and Methylprednisolone on COVID- 19 Patients’Consequence: A Non- Randomized, Double-Blinded, Controlled Study 研究β-干扰素和甲基强的松龙治疗COVID- 19患者的疗效:一项非随机、双盲、对照研究

Journal of Advances in Medical and Biomedical Research Pub Date : 2022-10-01 DOI: 10.30699/jambs.30.143.486

Roghaye Razavi, N. Namiranian, M. Samet, Fatemeh Aghaei Meybodi

{"title":"Investigating the Therapeutic Effect of β-Interferon and Methylprednisolone on COVID- 19 Patients’Consequence: A Non- Randomized, Double-Blinded, Controlled Study","authors":"Roghaye Razavi, N. Namiranian, M. Samet, Fatemeh Aghaei Meybodi","doi":"10.30699/jambs.30.143.486","DOIUrl":"https://doi.org/10.30699/jambs.30.143.486","url":null,"abstract":"Background & Objective: Due to the severe inflammatory conditions and cytokine storm in COVID-19 disease, corticosteroids are used worldwide as adjunctive therapy for these patients due to their anti-inflammatory effects. However, due to limited and inconsistent information about the effectiveness of this drug, this study aimed to investigate the benefit of combined use of beta-interferon and methylprednisolone in patients with COVID-19. Material(s) and Method(s): 57 patients infected with SARS coronavirus 2 underwent treatment. Laboratory parameters, hospitalization duration, and clinical outcomes in these patients were studied. Result(s): Statistical analysis showed no correlation between combination therapy with the hospitalization duration (P=0.22) and mortality (P=0.48). Also, the findings of this study showed that the ESR level in patients receiving combination therapy with methylprednisolone and interferon beta decreased significantly at the end of the intervention (P=0.0001). At the end of the study, the levels of neutrophils (P=0.001) and lymphocytes (P-value=0.0001) in the blood in the interferon group showed a significant change. Conclusion(s): This study showed no effect of adding methylprednisolone and interferon beta to the treatment protocol on mortality in the patients. Copyright © 2022, Zanjan University of Medical Sciences and Health Services. All rights reserved.","PeriodicalId":36550,"journal":{"name":"Journal of Advances in Medical and Biomedical Research","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43932526","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Frequency of Consanguinity and Its Related Factors in Parents of Children with Genetic Disorders 遗传性疾病患儿父母的血亲频率及其相关因素

Journal of Advances in Medical and Biomedical Research Pub Date : 2022-10-01 DOI: 10.30699/jambs.30.143.501

Sajjad Biglari, A. Biglari, S. Mazloomzadeh

{"title":"The Frequency of Consanguinity and Its Related Factors in Parents of Children with Genetic Disorders","authors":"Sajjad Biglari, A. Biglari, S. Mazloomzadeh","doi":"10.30699/jambs.30.143.501","DOIUrl":"https://doi.org/10.30699/jambs.30.143.501","url":null,"abstract":"10.30699/jambs.30.143.501 Background & Objective: Consanguinity increases the incidence of genetic disorders. The frequency of consanguinity varies in different societies. There was no data regarding the frequency of consanguinity in Zanjan province. This study aimed to describe the prevalence of consanguineous unions in the parents of children with genetic disorders and its related factors in Zanjan, Iran. Materials & Methods: This cross-sectional study included children with genetic diseases referring to the medical genetics clinic in Zanjan's Musavi Hospital during 2014-2018. Data including consanguineous unions in families (up to three previous generations), types of genetic illnesses, child and parents' age, parental educational level, and occupation were collected and analyzed using descriptive statistics, independent t-test, and chi-square test. Results: Of the 87 children, 41 (47.7%) were male, and 50 (59.5%) resided in urban areas. The mean age of the children was 6.5 years. The educational level of 56.6% of fathers and 50.6% of mothers were highschool diploma or higher. The parents of 44 children (51.8%) had consanguineous unions. The most common type of consanguineous union was between first cousins. The nature of the genetic disorders in 63 (72.4%) of children was molecular. The proportion of consanguinity was significantly higher in parents of children with molecular than chromosomal disorder (P<0.0001). Consanguinity had also a significant relationship with the children's age (P=0.04). Conclusion: This study's outcomes illustrate that parents of more than half of children with genetic disorders had consanguinity and the frequency of consanguinity was more common in parents of children with molecular disease than chromosomal disorder. Given that, it is necessary to avoid consanguineous marriage as much as possible, and families with a history of molecular genetic disease, should be informed of the possible consequences.","PeriodicalId":36550,"journal":{"name":"Journal of Advances in Medical and Biomedical Research","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46740661","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Rheum turkestanicum Janisch Root Extract Mitigates 6-OHDA-Induced Neuronal Toxicity Against Human Neuroblastoma SH-SY5Y Cells 大黄根提取物减轻6-羟多巴胺诱导的对人神经母细胞瘤SH-SY5Y细胞的神经毒性

Journal of Advances in Medical and Biomedical Research Pub Date : 2022-10-01 DOI: 10.30699/jambs.30.143.553

Roghayeh Rashidi, A. Afshari, H. Mollazadeh, A. Hosseini

{"title":"Rheum turkestanicum Janisch Root Extract Mitigates 6-OHDA-Induced Neuronal Toxicity Against Human Neuroblastoma SH-SY5Y Cells","authors":"Roghayeh Rashidi, A. Afshari, H. Mollazadeh, A. Hosseini","doi":"10.30699/jambs.30.143.553","DOIUrl":"https://doi.org/10.30699/jambs.30.143.553","url":null,"abstract":"10.30699/jambs.30.143.553 Background & Objective: Rheum turkestanicum ( R. turkestanicum ) has been known to reduce inflammation and has antioxidant properties such as protective effect in neurons. This study aimed to determine the effects of R. turkestanicum on neuronal toxicity induced by the pro-parkinsonian neurotoxin 6-hydroxydopamine (6-OHDA) in neuroblastoma SH-SY5Y cells. Materials & Methods: MTT and DNA fragmentation by PI staining (sub-G1 peak) assays were used to determine cell viability and induction of apoptosis, respectively. Fluorimetry methods measured lipid peroxidation malondialdehyde (MDA) and reactive oxygen species (ROS) levels. The amount of glutathione (GSH) and the activity of superoxide dismutase (SOD) were measured by DTNB (5, 5′ -Dithiobis (2-nitrobenzoic acid) and pyrogallol respectively. Results: Pretreatment with 12.5 to 100 μg/mL of R. turkestanicum extract for 24 hours attenuated 6- OHDA (final concentration 42.5 μg/mL) -induced cytotoxicity. Also, the pretreatment of SH-SY5Y cells with R. turkestanicum inhibited 6-OHDA-stimulated apoptosis in a dose-dependent manner. Additionally, R. turkestanicum extract repressed 6-OHDA-induced oxidative stress as measured by the MDA, ROS, GSH, and SOD levels. Conclusion: The findings suggest that R. turkestanicum extract has neuroprotective activity on 6-OHDA-induced neuronal toxicity of neuroblastoma cells.","PeriodicalId":36550,"journal":{"name":"Journal of Advances in Medical and Biomedical Research","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46747541","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Association of Physical Activity level with the COVID-19 Incidence in Patients with Type 2 Diabetes 2型糖尿病患者体力活动水平与COVID-19发病率的关系

Journal of Advances in Medical and Biomedical Research Pub Date : 2022-10-01 DOI: 10.30699/jambs.30.143.513

R. Hoseini, Z. Hoseini, Elahe Bahmani, Mahsa Ahmadi Darmian

{"title":"Association of Physical Activity level with the COVID-19 Incidence in Patients with Type 2 Diabetes","authors":"R. Hoseini, Z. Hoseini, Elahe Bahmani, Mahsa Ahmadi Darmian","doi":"10.30699/jambs.30.143.513","DOIUrl":"https://doi.org/10.30699/jambs.30.143.513","url":null,"abstract":"Background & Objective: Type 2 Diabetes Mellitus (T2DM) is among the epidemic metabolic chronic diseases that attenuates physiological functions, including hematopoiesis and immune responses, developing a higher risk of COVID-19 incidence. This study purposed to study the association of physical activity level (PAL) with the COVID-19 incidence in patients with T2DM. Material(s) and Method(s): All patients with T2DM from Kermanshah (Iran) were among the statistical population of this study. Using Cochran's formula, 424 patients with T2DM (220 women, 224 men) were chosen. The International Physical Activity Questionnaire (IPAQ) assessed the PAL. To compare the variables between men and women the independent t-test was used. Also, to evaluate the relationship between variables the Pearson correlation coefficient test was used (SPSS software version 24) at a significant level of (P<=0.05). Result(s): Based on the results, no significant difference was observed in PAL and COVID-19 incidence between women and men (P=0.342). Also, a significant positive relationship was observed between low and high PAL with COVID-19 incidence in men (r=0.638, P=0.039;r=0.231, P=0.048) and women (r=0.728, P=0.011;r=0.331, P=0.018), respectively. However, there was a significant inverse relationship between the moderate PAL and the incidence of COVID-19 among women (r=-0.791, P=0.021) and men (r=-0.731, P=0.029). Conclusion(s): Most of the patients with T2DM had an inadequate PAL. Compared with low and high PALs, moderate PAL might have lowered the incidence of COVID-19 more effectively. Finally, a moderate PAL is recommended to patients with T2DM to boost the immune system and prevent COVID-19 infection than high or low PAL. Copyright © 2022, Rastegar Hoseini et al.","PeriodicalId":36550,"journal":{"name":"Journal of Advances in Medical and Biomedical Research","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42516585","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Repair of a Scalp Defect with Punctate Skull Fenestration and Skin Grafting Puntate颅骨开窗植皮修复头皮缺损

Journal of Advances in Medical and Biomedical Research Pub Date : 2022-10-01 DOI: 10.30699/jambs.30.143.566

M. Ghoncheh, Forood Salehi

引用次数: 0

Evaluation of Probiotic Properties of Lactobacilli in Breast Milk and Their Inhibitory Effect on Pathogenic Bacteria of the Gastrointestinal Tracts 母乳乳酸菌益生菌特性的评价及其对胃肠道致病菌的抑制作用

Journal of Advances in Medical and Biomedical Research Pub Date : 2022-10-01 DOI: 10.30699/jambs.30.143.536

M. Soltan-dallal, Z. Rajabi, Sarah SHARİFİ YAZDİ, Parisa Torabi-Bonab, Alireza Monadi Sefidan, Sina Sharifi Yazdi

{"title":"Evaluation of Probiotic Properties of Lactobacilli in Breast Milk and Their Inhibitory Effect on Pathogenic Bacteria of the Gastrointestinal Tracts","authors":"M. Soltan-dallal, Z. Rajabi, Sarah SHARİFİ YAZDİ, Parisa Torabi-Bonab, Alireza Monadi Sefidan, Sina Sharifi Yazdi","doi":"10.30699/jambs.30.143.536","DOIUrl":"https://doi.org/10.30699/jambs.30.143.536","url":null,"abstract":"10.30699/jambs.30.143.536 Background & Objective: Breast milk contains nutrients such as carbohydrates, essential fatty acids, proteins, vitamins, minerals, and a source of communal bacteria with probiotic potential that is very effective in the prevention and treatment of neonatal infections. The aim of this study was the evaluation of probiotic properties of lactobacilli in breast milk and their inhibitory effect on pathogenic bacteria of the gastrointestinal tracts. Materials & Methods: In a cross-sectional descriptive study, during 10 months from January to October 2018, 100 breast milk samples were collected by referring to health centers after isolation. Lactobacilli strains were evaluated based on morphological characteristics, catalase, and hot staining tests, survival tests in acidic conditions, and bile salt tolerance to evaluate probiotic properties. Antibiotic resistance of probiotic strains and ability to inhibit pathogenic bacteria was evaluated by well method and growth inhibition zone. Results: 122 lactobacilli belonging to 12 species were identified from 100 samples of breast milk by phototypical methods. The predominant species belonging to casei and other lactobacilli were Fermentum, Plantarum, and Gasseri, respectively.The highest antibiotic resistance was related to vancomycin (63.15%). The 3 isolates L4, L14 and L16 were able to strongly inhibit all the studied gastrointestinal pathogens. Conclusion: Breast milk is a rich source of beneficial probiotic lactobacilli, which can be useful in breast milk for infants who are not breastfed to prevent neonatal infections.","PeriodicalId":36550,"journal":{"name":"Journal of Advances in Medical and Biomedical Research","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43039545","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigation of Gold Nanoparticles Effects in Radiation Therapy of Cancer: A Systematic Review 金纳米粒子在癌症放射治疗中的作用研究：系统综述

Journal of Advances in Medical and Biomedical Research Pub Date : 2022-09-01 DOI: 10.30699/jambs.30.142.388

H. Khosravi, A. Doosti-Irani, H. Bouraghi, S. Nikzad

引用次数: 2