Louis Groignet, David Dellemme, Quentin Duez, Afaf Fizazi, Jean-Marie Colet, Patrick Brocorens, Mathieu Surin, Pascal Gerbaux and Julien De Winter*,
{"title":"Impact of Post-Translational Succination on Small Ubiquitin-Like Modifier 1 Structure: A Dual Approach Combining Gas Phase and Solution Studies","authors":"Louis Groignet, David Dellemme, Quentin Duez, Afaf Fizazi, Jean-Marie Colet, Patrick Brocorens, Mathieu Surin, Pascal Gerbaux and Julien De Winter*, ","doi":"10.1021/acsptsci.5c00259","DOIUrl":"https://doi.org/10.1021/acsptsci.5c00259","url":null,"abstract":"<p >SUMO1 (small ubiquitin-like modifier 1) is a key protein involved in the post-translational modification of a wide range of substrate proteins. SUMOylation plays a pivotal role in regulating various cellular processes such as protein localization, functional modulation, and complex formation. Notably, SUMO1 contains a cysteine residue in its sequence, making it susceptible to succination, i.e., a Michael addition of cysteine onto fumarate, forming a succinated cysteine. This could occur under conditions of elevated intracellular fumarate concentration, a hallmark of metabolic dysregulation. To investigate this hypothesis, we employ a multidisciplinary approach integrating advanced analytical techniques such as mass spectrometry (including liquid chromatography, ion mobility spectrometry, and hydrogen–deuterium exchange experiments), circular dichroism spectroscopy, and molecular dynamics simulations. We demonstrate that SUMO1 undergoes succination <i>in vitro</i>, leading to important conformational changes. These findings provide insights into the susceptibility of SUMO1 to metabolic alterations.</p>","PeriodicalId":36426,"journal":{"name":"ACS Pharmacology and Translational Science","volume":"8 8","pages":"2683–2693"},"PeriodicalIF":3.7,"publicationDate":"2025-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144806207","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ketaki Deshpande, Varun Sai Tadimarri, Juliette Ramirez-Rangel, Shrikrishnan Sankaran and Sara Trujillo*,
{"title":"","authors":"Ketaki Deshpande, Varun Sai Tadimarri, Juliette Ramirez-Rangel, Shrikrishnan Sankaran and Sara Trujillo*, ","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":36426,"journal":{"name":"ACS Pharmacology and Translational Science","volume":"8 7","pages":"XXX-XXX XXX-XXX"},"PeriodicalIF":4.9,"publicationDate":"2025-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/pdf/10.1021/acsptsci.5c00216","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144595286","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Levi N. Kanu*, Amit Chatterjee, Mohamed Y. Mahmoud, Sushma V. Mudigunda, Shweta Gupta, Vincent Yeung, Nikolay Boychev, Xinyao Hu, Amy E. Ross and Joseph B. Ciolino,
{"title":"","authors":"Levi N. Kanu*, Amit Chatterjee, Mohamed Y. Mahmoud, Sushma V. Mudigunda, Shweta Gupta, Vincent Yeung, Nikolay Boychev, Xinyao Hu, Amy E. Ross and Joseph B. Ciolino, ","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":36426,"journal":{"name":"ACS Pharmacology and Translational Science","volume":"8 7","pages":"XXX-XXX XXX-XXX"},"PeriodicalIF":4.9,"publicationDate":"2025-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/pdf/10.1021/acsptsci.5c00287","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144595298","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Anne Chenchar, Vitoria Mattos Pereira, Anisha Apte, Bailey D. Lyttle, Alyssa E. Vaughn, Tanner Lehmann, James R. Bardill, Carlos Zgheib, Kenneth W. Liechty, Kelly C. S. Roballo, Zhaojie Zhang, Brenda M. Alexander* and Sreejayan Nair*,
{"title":"","authors":"Anne Chenchar, Vitoria Mattos Pereira, Anisha Apte, Bailey D. Lyttle, Alyssa E. Vaughn, Tanner Lehmann, James R. Bardill, Carlos Zgheib, Kenneth W. Liechty, Kelly C. S. Roballo, Zhaojie Zhang, Brenda M. Alexander* and Sreejayan Nair*, ","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":36426,"journal":{"name":"ACS Pharmacology and Translational Science","volume":"8 7","pages":"XXX-XXX XXX-XXX"},"PeriodicalIF":4.9,"publicationDate":"2025-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://pubs.acs.org/doi/pdf/10.1021/acsptsci.5c00295","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144595269","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}