Journal of Translational Autoimmunity最新文献

{"title":"A symposium update on the key perspectives in systemic sclerosis and systemic lupus erythematosus","authors":"Yves Renaudineau ,&nbsp;Christian M. Hedrich ,&nbsp;Jean-Charles Guery ,&nbsp;Makoto Miyara ,&nbsp;Jan Damoiseaux ,&nbsp;Luc Mouthon ,&nbsp;Gregory Pugnet ,&nbsp;Stanislas Faguer ,&nbsp;Antoine Delpuech ,&nbsp;Julie Bellière ,&nbsp;Laurent Sailler ,&nbsp;Bénédicte Puissant ,&nbsp;Mathieu Fusaro","doi":"10.1016/j.jtauto.2026.100379","DOIUrl":"10.1016/j.jtauto.2026.100379","url":null,"abstract":"<div><div>The fourth LBMR-Tim (Toulouse Referral Medical Laboratory of Immunology) symposium was convened on December 15th, 2025, in Toulouse, France, to discuss recent advances in the understanding and management of systemic sclerosis (SSc) and systemic lupus erythematosus (SLE). Pathophysiological mechanisms underlying SSc and SLE were discussed from a genetic perspective, with particular emphasis on the X-chromosomal <em>TLR7</em>/<em>TLR8</em> locus and the interferon signaling pathway. Cellular aspects were explored, highlighting the critical roles of regulatory T cells (Tregs), exhausted T cells, macrophage polarization, and endothelial cells. At the translational research frontier, significant initiatives are underway within the framework of the European Autoimmunity Standardization Initiative (EASI) aimed at enhancing routine biomarker application for diagnosis, disease monitoring, and prognostic considerations. Emerging biomarker candidates promise potential for improving prognostic assessment and follow-up in lupus nephritis (e.g., urinary sCD163/creatinine ratio), cardiovascular complications and vasculopathy associated with SSc (e.g., dephosphorylated-uncarboxylated matrix Gla protein [dp-ucMGP] and endothelial progenitor cells), as well as therapeutic response evaluation (e.g., IGRA-PHA assays and proteomic methodologies). Therapeutically, a paradigm shift is underway with the development of efficacious mono- and multi-targeted antibody treatments alongside cellular therapies designed to eliminate B cells through chimeric antigen receptor (CAR) T cells or to re-establish immune regulation through Treg restoration. The integration of these therapeutic modalities necessitates further investigation to optimize individualized patient selection and management strategies. The multidisciplinary expert panel advocates for a comprehensive approach encompassing fundamental science, translational research, clinical expertise, and therapeutic innovation to advance in the management of these two complex syndromes.</div></div>","PeriodicalId":36425,"journal":{"name":"Journal of Translational Autoimmunity","volume":"12 ","pages":"Article 100379"},"PeriodicalIF":3.6,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148184132","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"T cell proliferative response to a homocitrullinated peptide correlates with joint pathology in collagen induced arthritis","authors":"Jaspreet Kaur ,&nbsp;Sofya Ulanova ,&nbsp;Ewa Cairns ,&nbsp;Lillian Barra","doi":"10.1016/j.jtauto.2025.100345","DOIUrl":"10.1016/j.jtauto.2025.100345","url":null,"abstract":"<div><div>Rheumatoid arthritis (RA) is characterized by autoimmune responses against citrullinated and homocitrullinated proteins/peptides (CitP and HomoCitP respectively), resulting in joint pain, inflammation, and damage. Although the collagen induced arthritis (CIA) model shares pathological features with RA, it remains unclear whether this model is suitable for studying RA-specific immune responses. Therefore, we investigated whether immune responses to CitP and HomoCitP influence arthritis severity in CIA.</div><div>Male DBA/1J mice were immunized with bovine type II collagen (CII) (N = 58) or PBS (N = 14) in adjuvant. Arthritis was assessed by clinical arthritis score, caliper measurements of joint swelling, von Frey pain testing, micro-CT, histopathology, and immunofluorescence. Splenocyte proliferation was measured using flow cytometry and serum antibodies via ELISAs.</div><div>Although anti-bovine and anti-mouse CII IgG developed in all bovine CII-immunized mice, only 31 % developed clinical arthritis. Arthritis closely resembled RA pathology, including joint pain, reduced bone mineral density, and histopathological damage. Immunofluorescence showed higher CitP and HomoCitP in joints from arthritic vs. PBS-injected mice. At day 49 post primary immunization, HomoCitP, but not CitP, stimulation induced higher proliferation in splenic T cells from arthritic mice compared to controls and strongly correlated with joint swelling, pain, and histopathology, as well as with serum anti-CII IgG. Anti-HomoCitP IgG were rare, and anti-CitP IgG were undetected, in arthritic mice.</div><div>This study identifies RA-specific antigens and immune responses to HomoCitP as potentially relevant in CIA. These results support the use of CIA to study RA-specific immunopathogenic mechanisms and highlight HomoCitP-specific T cells as potential contributors to disease.</div></div>","PeriodicalId":36425,"journal":{"name":"Journal of Translational Autoimmunity","volume":"12 ","pages":"Article 100345"},"PeriodicalIF":3.6,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145926250","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antinuclear antibody targets in autoimmune hepatitis and drug-induced liver injury: Diagnostic relevance of nucleosome antibodies","authors":"Mirjam Kolev ,&nbsp;Michèle Freiburghaus ,&nbsp;Guido Stirnimann ,&nbsp;Henning Nilius ,&nbsp;Martin Wartenberg ,&nbsp;Juliette Schlatter ,&nbsp;Michael Nagler ,&nbsp;Michael P. Horn ,&nbsp;Nasser Semmo","doi":"10.1016/j.jtauto.2026.100354","DOIUrl":"10.1016/j.jtauto.2026.100354","url":null,"abstract":"<div><h3>Background &amp; Aims</h3><div>Anti-nuclear antibodies (ANA) are detected in 70–100 % of patients with autoimmune hepatitis (AIH) and in 22–33 % of patients with drug-induced liver injury (DILI). However, little is known about the specific antigens which they bind to. This study aimed to investigate the antigen specificity of ANA in patients with AIH and DILI and to assess the diagnostic value of these antibodies in differentiating between the two diseases.</div></div><div><h3>Methods</h3><div>We performed a retrospective, cross-sectional analysis of ANA-positive patients with AIH or DILI treated at a tertiary referral center. ANA patterns were determined by indirect immunofluorescence, followed by antigen-specific characterization using ELISA.</div></div><div><h3>Results</h3><div>A total of 81 patients were included (54 AIH, 27 DILI). A homogeneous ANA pattern was more frequently observed in AIH (75.9 %) than in DILI (48.1 %). Among patients with a homogeneous pattern, anti-nucleosome antibodies were present in 51.2 % of AIH patients and 15.4 % of DILI patients, yielding a specificity of 84.6 % (95 % confidence interval (CI) 54.6–98.1) and a sensitivity of 51.2 % (95 % CI: 35.1–67.1). Anti-nucleosome antibodies demonstrated the highest diagnostic accuracy in this subgroup (area under the curve (AUROC): 0.85; 95 % CI: 0.70–1.00), outperforming or equaling Immunoglobulin G (IgG) (AUROC: 0.85; 95 % CI: 0.74, 0.95), anti-F-actin antibodies (AUROC: 0.81; 95 % CI: 0.69, 0.93) and anti-smooth muscle antibodies (SMA) (AUROC: 0.80; 95 % CI: 0.67, 0.92).</div></div><div><h3>Conclusions</h3><div>In ANA-positive patients with a homogeneous ANA pattern, anti-nucleosome antibodies provide high diagnostic accuracy in distinguishing AIH from DILI. These findings suggest that anti-nucleosome antibodies may aid in the diagnostic workup of ANA-positive liver injury.</div></div>","PeriodicalId":36425,"journal":{"name":"Journal of Translational Autoimmunity","volume":"12 ","pages":"Article 100354"},"PeriodicalIF":3.6,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146167296","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"How loss-of-function mutations in IFIH1 contribute to infectious and/or inflammatory disease – a systematic review","authors":"Isabelle Ince ,&nbsp;Francesca Sposito ,&nbsp;Amandine Charras ,&nbsp;Liza J. McCann ,&nbsp;Christian M. Hedrich","doi":"10.1016/j.jtauto.2026.100353","DOIUrl":"10.1016/j.jtauto.2026.100353","url":null,"abstract":"<div><div>The <em>IFIH1</em> gene encodes for the cytoplasmic innate immune receptor <em>Melanoma Differentiation-Associated protein 5</em> (MDA5) that detects viral double-stranded RNA to initiate type I interferon (IFN) responses. While gain-of-function mutations in <em>IFIH1</em> have been linked with systemic inflammatory diseases, loss-of-function remains less well understood.</div><div>This systematic review, following <em>Preferred Reporting Items for Systematic reviews and Meta-Analyses</em> (PRISMA) guidance, explored how <em>IFIH1</em>/MDA5 loss-of-function affects susceptibility to virus infections and/or contributes to inflammatory diseases.</div><div>Sixteen loss-of-function variants affecting <em>IFIH1</em> were discussed across 33 studies. Loss-of-function variants were consistently associated with increased susceptibility and/or severity of <strong>virus infections</strong>, including severe acute respiratory syndrome coronavirus (SARS-CoV2) and human immunodeficiency virus (HIV). Several rare biallelic <em>IFIH1</em> mutations lead to profound immunodeficiency, while heterozygous mutations associate with milder clinical presentations. Likely through dampening IFN responses, several variants <strong>protect from the development of inflammatory diseases, including</strong> type 1 diabetes and hypothyroidism. However, <em>IFIH1</em> deficiency is also implicated in the development of inflammatory diseases, including i<strong>nflammatory bowel disease. Moreover,</strong> the presence of <strong>inactivating</strong> anti-MDA5 antibodies may alter the clinical phenotypes and prognosis of dermatomyositis and infections with SARS-CoV2. Though their exact impact on MDA5 function has not been confirmed experimentally, anti-MDA5 antibodies may result in loss-of-function and impaired host defence against viruses.</div><div>Loss of <em>IFIH1</em>/MDA5 activity has diverse effects on anti-viral immunity, associated damage and susceptibility to inflammatory disease, but also protection against organ-specific immune-mediated pathology. Findings highlight the importance of <em>IFIH1</em> in immune regulation and warrant future studies exploring its potential as a diagnostic and therapeutic target.</div></div>","PeriodicalId":36425,"journal":{"name":"Journal of Translational Autoimmunity","volume":"12 ","pages":"Article 100353"},"PeriodicalIF":3.6,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146173501","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Autoantibodies reactive with glomerular endothelial cells and podocytes in patients with membranous nephropathy","authors":"Vojtech Petr ,&nbsp;Shrey Purohit ,&nbsp;Felix Poppelaars ,&nbsp;Brandon Renner ,&nbsp;Jennifer Laskowski ,&nbsp;Russell Whelan ,&nbsp;Liudmila Kulik ,&nbsp;Jessica Kendrick ,&nbsp;Ashley Frazer-Abel ,&nbsp;Diana Jalal ,&nbsp;Barbara Marcolin ,&nbsp;Isabelle Schmelzer ,&nbsp;Hanna Debiec ,&nbsp;Pierre Ronco ,&nbsp;Moin A. Saleem ,&nbsp;Simon C. Satchell ,&nbsp;Joshua M. Thurman","doi":"10.1016/j.jtauto.2025.100342","DOIUrl":"10.1016/j.jtauto.2025.100342","url":null,"abstract":"<div><h3>Rationale &amp; objective</h3><div>Membranous nephropathy (MN) is a glomerular disease caused by autoantibodies reactive with podocyte antigens. The most common antigen is the M-type phospholipase A2 receptor (PLA2R), but autoantibodies to other podocyte antigens have also been identified. Investigators have reported elevated levels of complement fragments in plasma. However, most complement fragments generated on podocytes are likely to pass into the urine and not enter the bloodstream. Further, anti-PLA2R antibodies are usually IgG4 subclass and do not activate the classical pathway of complement. To look for additional autoantibodies capable of generating endovascular complement fragments, we examined whether MN patients have antibodies reactive with endothelial cell antigens.</div></div><div><h3>Study design</h3><div>Retrospective cohort study.</div></div><div><h3>Setting &amp; participants</h3><div>We analyzed plasma samples from 64 patients with MN, and results were compared to healthy controls and patients with chronic kidney disease.</div></div><div><h3>Exposure</h3><div>Plasma and urine complement activation fragments, glomerular endothelial cell and podocyte antibody binding assays, anti-cardiolipin antibody enzyme linked immunosorbent assay.</div></div><div><h3>Outcome</h3><div>Proteinuria, estimated glomerular filtration rate.</div></div><div><h3>Analytical approach</h3><div>Groups were compared with Wilcoxon, Kruskal-Wallis or chi-square tests. Correlations were performed using Pearson's correlation.</div></div><div><h3>Results</h3><div>Plasma C3a, C4a, C5a, and sC5b-9 levels were elevated in MN patients. Some patients had IgG reacted with glomerular endothelial cells or with podocytes. These antibodies were seen in distinct subsets of patients and did not correlate with the presence of anti-PLA2R antibodies. Higher titers of anti-glomerular endothelial cell antibodies correlated with systemic complement activation, seen by sC5b-9, and disease severity, determined by proteinuria. Anti-cardiolipin IgG levels associated with proteinuria.</div></div><div><h3>Limitations</h3><div>Assays used immortalized cell lines, and target antigens have not yet been identified.</div></div><div><h3>Conclusions</h3><div>MN is a disease of autoimmunity directed against podocyte antigens, but some patients may also produce autoantibodies that target antigens on glomerular endothelial cells. The level of these antibodies correlates with adverse clinical findings.</div></div>","PeriodicalId":36425,"journal":{"name":"Journal of Translational Autoimmunity","volume":"12 ","pages":"Article 100342"},"PeriodicalIF":3.6,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145791081","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Downregulated ECHS1 and HADH-mediated fatty acid β-oxidation contributes to mitochondrial dysfunction in salivary glands of Sjögren's syndrome","authors":"Min Zhou ,&nbsp;Yu Liu ,&nbsp;Jinxia He ,&nbsp;Chengyin Li ,&nbsp;Bin Wu","doi":"10.1016/j.jtauto.2026.100350","DOIUrl":"10.1016/j.jtauto.2026.100350","url":null,"abstract":"&lt;div&gt;&lt;h3&gt;Background&lt;/h3&gt;&lt;div&gt;Sjögren's syndrome (SS) is a common autoimmune disease characterized clinically by dry mouth and dry eyes resulting from impaired exocrine gland function. However, its precise pathogenesis remains incompletely understood. Mitochondria, as cellular energy hubs, maintain homeostasis via fatty acid β-oxidation (FAO), the tricarboxylic acid (TCA) cycle, and oxidative phosphorylation. ECHS1 and HADH are key enzymes in the mitochondria that catalyze the FAO process, playing crucial regulatory roles in cellular energy metabolic balance. This study aims to systematically investigate alterations in mitochondrial structure and function in the salivary glands of SS patients and mice and to elucidate the potential mechanisms of ECHS1 and HADH in SS-related mitochondrial dysfunction.&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Methods&lt;/h3&gt;&lt;div&gt;To systematically evaluate the association between mitochondrial abnormalities and salivary gland secretory function in SS, human salivary gland samples from SS patients and non-SS controls, alongside non-obese diabetic (NOD) mice and ICR-control mice, were analyzed. Mitochondrial ultrastructure was examined via transmission electron microscopy (TEM), and functional indicators were measured. Transcriptome sequencing identified differentially expressed genes (DEGs), which were cross-referenced with MitoCarta3.0 to pinpoint mitochondrial-related DEGs (MR-DEGs). KEGG pathway enrichment analysis and GO functional annotation were performed. A protein-protein interaction (PPI) network was then constructed using the TCMNP database to identify key hub genes. Finally, Key hub genes ECHS1 and HADH were validated at both the transcriptional and protein levels using the GEO dataset (GSE40611), qPCR, and immunofluorescence.&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Results&lt;/h3&gt;&lt;div&gt;The results revealed significant mitochondrial structural and functional abnormalities in both SS patients with salivary gland dysfunction and NOD mice. Compared to controls, salivary gland mitochondria in NOD mice exhibited marked ultrastructural damage, including swelling and cristae fragmentation, accompanied by increased ROS levels and decreased mitochondrial membrane potential. Transcriptomic analysis identified 81 MR-DEGs. KEGG enrichment analysis indicated these genes were significantly enriched in energy metabolism-related pathways such as Fatty acid biosynthesis, Fatty acid metabolism, and TCA cycle. GO analysis showed that MR-DEGs were primarily involved in biological processes like the fatty acid metabolic process, localized to the mitochondrial matrix, and possessed molecular functions such as ligase activity. PPI network analysis further screened seven hub genes (ECHS1, HADH, PCX, ACADM, ACACA, FASN, ACLY), among which ECHS1 and HADH exhibited the highest interaction score. Consistent results from GEO database analysis and qPCR validation demonstrated significantly downregulated mRNA expression levels of ECHS1 and HADH in the salivary glands of both SS patien","PeriodicalId":36425,"journal":{"name":"Journal of Translational Autoimmunity","volume":"12 ","pages":"Article 100350"},"PeriodicalIF":3.6,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146022432","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Liraglutide prevents lupus-associated diffuse alveolar hemorrhage via inhibiting lymphocyte infiltration and promoting macrophage M2 polarization","authors":"Li Jiang ,&nbsp;Liting He ,&nbsp;Duo Li ,&nbsp;Xin Luo ,&nbsp;Ming Yang ,&nbsp;Haijing Wu ,&nbsp;Hai Long","doi":"10.1016/j.jtauto.2026.100349","DOIUrl":"10.1016/j.jtauto.2026.100349","url":null,"abstract":"<div><h3>Objectives</h3><div>This study aimed to explore the prophylactic and therapeutic role of liraglutide for lupus-associated diffuse alveolar hemorrhage (DAH).</div></div><div><h3>Methods</h3><div>A lupus-associated DAH model was established in 6-8-week-old female C57BL/6 mice via intraperitoneal injection of 0.5 mL pristane. Mice were randomized to receive daily intraperitoneal injections of either saline or liraglutide (2 mg/kg/day), starting either 14 days before or on the day of pristane injection, and continuing until 14 days post-injection. Body weight and blood glucose were monitored. Serum levels of anti-dsDNA, ANA, total IgM and IgG were quantified using ELISA. Lung tissues were collected for histopathological analysis with H&amp;E and Prussian blue staining, respectively, characterization of different types of immune cells infiltration with flow cytometry (t-SNE for subset dimensionality reduction) and immunofluorescence, and quantification of protein levels with Western blot.</div></div><div><h3>Results</h3><div>Liraglutide significantly alleviated pulmonary hemorrhage, as evidenced by reduced lung wet weight, incidence of lung hemorrhage, pulmonary hemosiderin-laden macrophages, and total serum IgG, without affecting body weight or glucose. Mechanistically, liraglutide treatment reduced pulmonary infiltration of multiple T and B lymphocyte subsets (e.g., CD4⁺, CD8⁺, B220⁺), while concurrently increasing alveolar macrophages and promoting M2 macrophage polarization. The phenotypic shift was demonstrated by increased M2 cell numbers, elevated CD206 expression, and downregulation of M1 marker CD86 and upregulation of M2 marker CD163 as confirmed at the protein level.</div></div><div><h3>Conclusion</h3><div>Liraglutide treatment effectively ameliorates lupus-associated DAH, potentially through modulation of T cells, B cells, and macrophages polarization, offering a novel prophylactic and therapeutic strategy for this fatal complication of lupus.</div></div>","PeriodicalId":36425,"journal":{"name":"Journal of Translational Autoimmunity","volume":"12 ","pages":"Article 100349"},"PeriodicalIF":3.6,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146022431","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identifying subtype-specific molecular pathways in Crohn’s disease through RNA-seq and protein–protein interaction network analysis","authors":"Sree Ishan Kolukula","doi":"10.1016/j.jtauto.2025.100337","DOIUrl":"10.1016/j.jtauto.2025.100337","url":null,"abstract":"<div><div>Crohn’s Disease (CD) is a chronic autoinflammatory disease of the gastrointestinal tract. Anatomical labels like Ileal Crohn’s Disease (ICD) and Colonic Crohn’s Disease (CCD) do not capture the molecular heterogeneity which contributes to trial and error therapy. This trial and error pattern costs patients who switch biologics higher annual expenses. We analyzed bulk RNA-seq from 2353 biopsies across two independent data sets (GSE193677 and GSE57945) using a standardized pipeline. Principal component analysis confirmed clear molecular separation between ICD and CCD samples. Differential expression modeling (DESeq2, FDR <span><math><mo>≤</mo></math></span> 0.05) identified the top 300 differentially expressed genes (DEGs) across subtype specific signatures. Pathway analysis confirmed known subtype biology, with ICD driven by autophagy-related processes and CCD by immune activation pathways. Subtype-specific PPI networks diverged sharply, with CKB driving barrier-related processes in ICD and SPP1 coordinating immune activation in CCD. Known CD susceptibility genes (e.g., NOD2, ATG16L1, IL23R) were recovered within leading-edge sets, supporting construct validity. Proteomic validation using ProteomeXchange PXD012284 confirmed concordant enrichment of ICD-associated autophagy and lysosomal modules and CCD-associated innate immune pathways at the protein level. Single-cell transcriptomic validation further localized leading-edge genes to epithelial lineages in ICD and to myeloid and glial populations in CCD, supporting cellular specificity of subtype programs. Together, these results indicate that ICD and CCD are biologically distinct at the transcriptome, proteome, and network levels. The prioritized hubs and pathways nominate tractable, subtype-specific hypotheses for prospective validation and provide a framework for precision therapeutics in CD.</div></div>","PeriodicalId":36425,"journal":{"name":"Journal of Translational Autoimmunity","volume":"12 ","pages":"Article 100337"},"PeriodicalIF":3.6,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145926251","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Autoantibody and biomarker detection in the follow-up of autoimmune diseases: state of the art and future perspectives","authors":"Jan Damoiseaux ,&nbsp;Yves Renaudineau","doi":"10.1016/j.jtauto.2025.100346","DOIUrl":"10.1016/j.jtauto.2025.100346","url":null,"abstract":"<div><div>Originally developed for the diagnosis of autoimmune diseases, the application of autoantibodies and related biomarkers has been extended in certain cases to monitor therapeutic efficacy and to predict disease recurrence and/or severity. Several critical considerations must be addressed prior to employing autoantibodies for monitoring purposes: (i) whether the autoantibody is pathogenic, as exemplified by anti-glomerular basement membrane and anti-acetylcholine receptor autoantibodies; (ii) whether the autoantibody level is essential for tracking disease activity, which may necessitate establishing prognostic thresholds and/or adapting detection methodologies; (iii) whether there is added value in combining autoantibodies, as demonstrated with anti-dsDNA and anti-chromatin antibodies in systemic lupus erythematosus; (iv) which immunoglobulin isotype is optimal for monitoring; and (v) whether alternative biomarkers exist that provide greater accuracy for patient follow-up. Additional issues remain unresolved, including appropriate intervals between measurements, intra- and inter-laboratory reproducibility, inter-assay variations, and ethnic variability, among others. To address these challenges, the European Autoimmunity Standardization Initiative (EASI) has proposed adapted strategies for utilizing autoantibodies in the longitudinal assessment of selected autoimmune diseases, as presented in this special issue.</div></div>","PeriodicalId":36425,"journal":{"name":"Journal of Translational Autoimmunity","volume":"12 ","pages":"Article 100346"},"PeriodicalIF":3.6,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145926254","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Female cancer incidence before and after diagnosis of primary Sjögren's disease: A retrospective cohort study","authors":"Bitte Sjöström ,&nbsp;Anders Bredberg ,&nbsp;Peter Olsson ,&nbsp;Susann Ullén ,&nbsp;Johannes H. van der Stoep ,&nbsp;Gunnel Henriksson","doi":"10.1016/j.jtauto.2026.100352","DOIUrl":"10.1016/j.jtauto.2026.100352","url":null,"abstract":"<div><div>In primary Sjögren's disease (pSjD), there is a well-documented increased risk of hematological malignancies, particularly lymphomas, whereas studies examining the incidence of solid tumors have often yielded conflicting results. Data concerning the incidence of breast and gynecological cancers are similarly inconsistent. The aim of this study was to further investigate the incidence of these so-called female cancers by assessing not only their occurrence following the diagnosis of pSjD but also prior to it. By linking the Malmö Sjögren's disease register with the Swedish National Cancer Register, 25 cases of female cancers were identified among patients with pSjD (mean follow-up time: 45.8 years), compared to 50.70 expected cases (SIR, 0.49; 95 % CI, 0.30–0.69; <em>P</em> &lt; 0.001). The incidence of female cancers was significantly reduced both prior to (<em>P</em> &lt; 0.001) and following (<em>P</em> &lt; 0.001) the diagnosis of pSjD. The decrease was most evident for breast cancer (SIR, 0.46; 95 % CI, 0.24–0.69; <em>P</em> &lt; 0.001). A reduction was also noted for ovarian cancer (SIR, 0.21; 95 % CI, 0.00–0.62; <em>P</em> &lt; 0.001); however, the reliability of this estimate is limited by the small number of observed cases. Our findings suggest a reduced risk of female cancers, primarily driven by a decrease in breast cancer incidence, not only following but also preceding the diagnosis of pSjD. These results, combined with the observation that autoantibodies characteristic of pSjD often emerge several years before clinical onset, raise the question of whether an autoimmune component may confer a protective effect against breast cancer in pSjD.</div></div>","PeriodicalId":36425,"journal":{"name":"Journal of Translational Autoimmunity","volume":"12 ","pages":"Article 100352"},"PeriodicalIF":3.6,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146077625","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}