Research in Plant Disease最新文献_第9页

Biological and Molecular Characterization of Tomato brown rugose fruit virus (ToBRFV) on Tomato Plants in the State of Palestine 巴勒斯坦番茄褐皱果病毒(ToBRFV)的生物学和分子特性

Research in Plant Disease Pub Date : 2022-06-30 DOI: 10.5423/rpd.2022.28.2.98

R. Jamous, S. Y. A. Zaitoun, O. Mallah, M. Ali-Shtayeh

{"title":"Biological and Molecular Characterization of Tomato brown rugose fruit virus (ToBRFV) on Tomato Plants in the State of Palestine","authors":"R. Jamous, S. Y. A. Zaitoun, O. Mallah, M. Ali-Shtayeh","doi":"10.5423/rpd.2022.28.2.98","DOIUrl":"https://doi.org/10.5423/rpd.2022.28.2.98","url":null,"abstract":"The incidence of Tomato brown rugose fruit virus (ToBRFV) and biological and molecular characterization of the Palestinian isolates of ToBRFV are described in this study. Symptomatic leaf samples obtained from Solanum lycopersicum L. (tomatoes) and Nicotiana tabacum L. (cultivated tobacco) plants were tested for tobamoviruses infection by reverse transcription polymerase chain reaction. Tomato leaf samples collected from Tulkarm and Qalqilia are infected with ToBRFV-PAL with an infection rate of 76% and 72.5%, respectively. Leaf samples collected from Jenin and Nablus were found to be mixed infected with ToBRFV-PAL and Tobacco mosaic virus (TMV) (100%). Sequence analysis of the ToBRFV-PAL genome showed that the net average nucleotide divergence between ToBRFV/F48-PAL strain and the Israeli and Turkish strains was 0.0026398±0.0006638 (±standard error of mean), while it was 0.0033066±0.0007433 between ToBRFV/F42-PAL and these two isolates. In the phylogenetic tree constructed with the complete genomic sequence, all the ToBRFV isolates were clustered together and formed a sister branch with the TMV. The sequenced Palestinian isolates of ToBRFV-PAL shared the highest nucleotide identity with the Israeli ToBRFV isolate suggesting that the virus was introduced to Palestine from Israel. The findings of this study enhance our understanding of the biological and molecular characteristics of ToBRFV which would help in the management of the disease.","PeriodicalId":36349,"journal":{"name":"Research in Plant Disease","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45360565","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Stem and Leaf Rot of Tomato Caused by Boeremia linicola 番茄布尔菌引起的茎叶腐病

Research in Plant Disease Pub Date : 2022-06-30 DOI: 10.5423/rpd.2022.28.2.108

Gyo-Bin Lee, Taek-Hyeon Oh, Jae-Taek Ryu, Wan-Gyu Kim

引用次数: 0

Biological Efficacy of Endophytic Bacillus velezensis CH-15 from Ginseng against Ginseng Root Rot Pathogens 人参内生威氏芽孢杆菌CH-15对人参根腐病病原菌的生物学效应

Research in Plant Disease Pub Date : 2022-03-31 DOI: 10.5423/rpd.2022.28.1.19

Dohyun Kim, Taiying Li, Jungkwan Lee, Seunghoon Lee

{"title":"Biological Efficacy of Endophytic Bacillus velezensis CH-15 from Ginseng against Ginseng Root Rot Pathogens","authors":"Dohyun Kim, Taiying Li, Jungkwan Lee, Seunghoon Lee","doi":"10.5423/rpd.2022.28.1.19","DOIUrl":"https://doi.org/10.5423/rpd.2022.28.1.19","url":null,"abstract":"Ginseng is an important medicinal plant cultivated in East Asia for thousands of years. It is typically cultivated in the same field for 4 to 6 years and is exposed to a variety of pathogens. Among them, ginseng root rot is the main reason that leads to the most severe losses. In this study, endophytic bacteria were isolated from healthy ginseng, and endophytes with antagonistic effect against ginseng root rot pathogens were screened out. Among the 17 strains, three carried antagonistic effect, and were resistant to radicicol that is a mycotoxin produced by ginseng root rot pathogens. Finally, Bacillus velezensis CH-15 was selected due to excellent antagonistic effect and radicicol resistance. When CH-15 was inoculated on ginseng root, it not only inhibited the mycelial growth of the pathogen, but also inhibited the progression of disease. CH-15 also carried biosynthetic genes for bacillomycin D, iturin A, bacilysin, and surfactin. In addition, CH-15 culture filtrate significantly inhibited the growth and conidial germination of pathogens. This study shows that endophytic bacterium CH-15 had antagonistic effect on ginseng root rot pathogens and inhibited the progression of ginseng root rot. We expected that this strain can be a microbial agent to suppress ginseng root rot.","PeriodicalId":36349,"journal":{"name":"Research in Plant Disease","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44911360","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Post-harvest Green Pea Pod Rot Caused by Sclerotinia sclerotiorum in Korea 韩国青豌豆菌核病引起的采后腐病

Research in Plant Disease Pub Date : 2022-03-31 DOI: 10.5423/rpd.2022.28.1.46

M. Aktaruzzaman, T. Afroz, Byung-Sup Kim

引用次数: 0

Incidence and Distribution of Barley yellow dwarf virus Infecting Oats in Korea 大麦黄矮病毒在韩国燕麦的发病及分布

Research in Plant Disease Pub Date : 2022-03-31 DOI: 10.5423/rpd.2022.28.1.32

Na-kyeong Kim, Hyo-jeong Lee, Sang-Min Kim, R. Jeong

引用次数: 1

Aromatic Agriculture: Volatile Compound-Based Plant Disease Diagnosis and Crop Protection 芳香农业:基于挥发性化合物的植物病害诊断和作物保护

Research in Plant Disease Pub Date : 2022-03-31 DOI: 10.5423/rpd.2022.28.1.1

M. Riu, Jin-Soo Son, Sang-Keun Oh, C. Ryu

{"title":"Aromatic Agriculture: Volatile Compound-Based Plant Disease Diagnosis and Crop Protection","authors":"M. Riu, Jin-Soo Son, Sang-Keun Oh, C. Ryu","doi":"10.5423/rpd.2022.28.1.1","DOIUrl":"https://doi.org/10.5423/rpd.2022.28.1.1","url":null,"abstract":"Volatiles exist ubiquitously in nature. Volatile compounds produced by plants and microorganisms confer inter-kingdom and intra-kingdom communications. Autoinducer signaling molecules from contact-based chemical communication, such as bacterial quorum sensing, are relayed through short distances. By contrast, biogenic volatiles derived from plant-microbe interactions generate long-distance (>20 cm) alarm signals for sensing harmful microorganisms. In this review, we discuss prior work on volatile compound-mediated diagnosis of plant diseases, and the use of volatile packaging and dispensing approaches for the biological control of fungi, bacteria, and viruses. In this regard, recent developments on technologies to analyze and detect microbial volatile compounds are introduced. Furthermore, we survey the chemical encapsulation, slow-release, and bio-nano techniques for volatile formulation and delivery that are expected to overcome limitations in the application of biogenic volatiles to modern agriculture. Collectively, technological advances in volatile compound detection, packaging, and delivery provide great potential for the implementation of ecologically-sound plant disease management strategies. We hope that this review will help farmers and young scientists understand the nature of microbial volatile compounds, and shift paradigms on disease diagnosis and management to aromatic (volatile-based) agriculture.","PeriodicalId":36349,"journal":{"name":"Research in Plant Disease","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43759823","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

First Report of Bacterial Wilt by Ralstonia pseudosolanacearum on Peanut in Korea 伪青枯菌在韩国花生上引起青春病的首次报告

Research in Plant Disease Pub Date : 2022-03-31 DOI: 10.5423/rpd.2022.28.1.54

S. Choi, Nam-Goo Kim, Sang-Min Kim, Bong-Choon Lee

引用次数: 0

Mutation of rpsL Gene in Streptomycin-Resistant Pseudomonas syringae pv. actinidiae Biovar 3 Strains Isolated from Korea 耐链霉素丁香假单胞菌rpsL基因突变研究。actinidiae Biovar 3韩国分离株

Research in Plant Disease Pub Date : 2022-03-31 DOI: 10.5423/rpd.2022.28.1.26

Young Sun Lee, Gyoung-Hee Kim, Y. Koh, J. Jung

{"title":"Mutation of rpsL Gene in Streptomycin-Resistant Pseudomonas syringae pv. actinidiae Biovar 3 Strains Isolated from Korea","authors":"Young Sun Lee, Gyoung-Hee Kim, Y. Koh, J. Jung","doi":"10.5423/rpd.2022.28.1.26","DOIUrl":"https://doi.org/10.5423/rpd.2022.28.1.26","url":null,"abstract":"Pseudomonas syringae pv. actinidiae (Psa) is the causal agent responsible for the bacterial canker disease of kiwifruit plants. Psa strains are divided into five different biovars based on genetic and biochemical characteristics. Among them, biovar 2 and 3 strains of Psa were isolated and have been causing widespread damages in Korea. One of the most effective ways to control Psa is to use an antibiotic such as streptomycin. However, Psa strains resistant to this antibiotic were isolated in Korea, and an earlier study revealed that the resistance in the biovar 2 is associated with strA-strB genes. This study aimed to determine the molecular resistance mechanism of Psa biovar 3 strains to streptomycin. Sequencing the rpsL gene encoding ribosomal protein S12 from three streptomycin-resistant strains screened in the laboratory revealed that a spontaneous mutation occurred either at codon 43 or 88. Meanwhile, in four streptomycin-resistant strains of Psa biovar 3 isolated from two kiwifruit orchards, a single nucleotide in codon 43 of the rpsL, which is AAA in streptomycin-sensitive strain, was substituted for AGA causing an amino acid change from lysine to arginine. The resistant mechanism in all biovar 3 strains obtained in Korea was identified as a mutation of the rpsL gene.","PeriodicalId":36349,"journal":{"name":"Research in Plant Disease","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45931534","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Shoot Rot of Spikenard Caused by Rhizoctonia solani AG-2-1 茄枯丝核菌AG-2-1致穗草茎腐病的研究

Research in Plant Disease Pub Date : 2022-03-31 DOI: 10.5423/rpd.2022.28.1.51

Youn-Gi Moon, H. Seo, K. Park, Wan-Gyu Kim

引用次数: 0

Patterns of the Occurrence of TYLCV and ToCV with Whitefly on Summer-Cultivated Tomato in Greenhouse in Gwangju, Gyeonggi Province 京畿道光州市温室栽培番茄TYLCV和ToCV与白蝇的发生规律

Research in Plant Disease Pub Date : 2022-03-31 DOI: 10.5423/rpd.2022.28.1.39

Y. Kwon, B. Cha, Mikyeong Kim

{"title":"Patterns of the Occurrence of TYLCV and ToCV with Whitefly on Summer-Cultivated Tomato in Greenhouse in Gwangju, Gyeonggi Province","authors":"Y. Kwon, B. Cha, Mikyeong Kim","doi":"10.5423/rpd.2022.28.1.39","DOIUrl":"https://doi.org/10.5423/rpd.2022.28.1.39","url":null,"abstract":"Patterns of occurrence of tomato yellow leaf curl virus (TYLCV) and tomato chlorosis virus (ToCV) with whitefly on summer-cultivated tomato in Gwangju-si, Gyeonggi Province were surveyed using multiplex reverse transcription-polymerase chain reaction in 2020. In addition, distribution of the whiteflies species and their viral transmission rates were investigated throughout the tomato growing season. The infection rates of TYLCV and ToCV increased sharply during harvest, and the single infection rates were 30.9% and 5.0%, respectively, with a mixed infection rate of the two viruses being the highest at 52.2%. Single infection with TYLCV and double infections with TYLCV and ToCV accounted for the majority with 83.1%. Bemisia tabaci were dominant over Trialeurodes vaporariorum in greenhouse grown plants, and all of the investigated B. tabaci biotypes were identified as Mediterranean (MED, formerly known as Q biotype). The transmission rate of TYLCV, detected in every sampled B. tabaci MED population, was 21.4%, and the mixed transmission rate with ToCV was 35.5%. Viruliferous MED whiteflies with ToCV showed a higher rate than that of T. vaporariorum. In the transplant stage, viruliferous rate of both TYLCV and ToCV of B. tabaci was 42.7%; this rate was highest in the harvest stage. In examination of tomato yield, the increase in the mixed infection rate of TYLCV and ToCV led to complete yield loss. When the mixed infection rate increased by 10%, the yield decreased by 405.4 kg/10a.","PeriodicalId":36349,"journal":{"name":"Research in Plant Disease","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46128284","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0