International Journal of Medicine and Biomedical Sciences最新文献

{"title":"Antibacterial and Antifungal Activities of Micromeria biflora (Leaves).","authors":"Muhammad Aurang Zeb, A. Wahab, N. Ullah, S. Pandey, T. Muhammad","doi":"10.55530/ijmbiosnepal.v1i1.7","DOIUrl":"https://doi.org/10.55530/ijmbiosnepal.v1i1.7","url":null,"abstract":"\u0000Background: Herbal medicines have been practiced for thousands of centuries by tribes all over the world. We aim to study extraction, fractionation, antibacterial and antifungal activity of micromeriabiflora. \u0000Methodology: Sample was washed properly with de-ionized water, solvents used (i.e. chloroform, ethyl acetate, n-hexane and methanol) were filtered and added. Some amounts of dehydrated reagents to purify the solvents before using. Shad dried plant powder was chopped and soaked in methanol for 10  days Dimethyl sulfoxide(DMSO) was used as solvent because it did not show any activity against bacteria and also it possess polar and non-polar groups therefore, in dimethyl sulfoxide most of the polar and non-polar compounds are soluble. The antibacterial bioassay was done by Agar Well Diffusion method, by measuring the zone of inhibition against the test microorganisms. Two fungal strains i.e. Alternaria, Fusariumoxysporium were used for antifungal activities. \u0000Results: At 5mg/mm the detailed spectrum of antibacterial activity of various fraction i.e. n-hexane, chloroform, ethyl acetate, and methanol was determined. All fractions showed activity against fungal species. Highest activity was shown by Chloroform fraction against Fusariumoxysporium giving wider zone of 10.2 (mm). Ethyl acetate fraction against Alternaria was very less active giving lowest value of 5.9 (mm). \u0000Conclusion: The results of antibacterial activity revealed that methanol show highest activity among all fractions against salmonella typhi. The methanol extract of Micromeriabiflora gave the widest zone of inhibition (16.1mm) against salmonella typhi using agar well diffusion. The results therefore established a good support for the use of Micromeriabiflora in traditional medicine. \u0000","PeriodicalId":359742,"journal":{"name":"International Journal of Medicine and Biomedical Sciences","volume":"42 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115345008","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Editorial Publication Matters","authors":"K. Sapkota","doi":"10.55530/ijmbiosnepal.v1i1.3","DOIUrl":"https://doi.org/10.55530/ijmbiosnepal.v1i1.3","url":null,"abstract":"International Journal of Medicine and Biomedical Sciences (IJMBioS) is a platform for researchers to publish their research across various disciplines. The journal has been initiated by academicians who specialize in different fields such as cell biology, biochemistry, medical chemistry, medical science, microbiology and public health to name but a few. The primary aim of the journal is to publish original research to enhance our current knowledge of medical, biomedical and public health sciences. \u0000A researcher spends a significant time in doing research. After the completion of the study, it is the time for the publication of the hard work. However, many researchers are reluctant to write and publish the research paper once the project is completed. The famous saying “publish or perish” is true because timely publication of research paper is essential to progress in their carriers. \u0000With the aim of promoting both students and scholars’ research, we have made a publication process much easier and faster. That is to say, a researcher will upload the final draft of the research paper. Once the research paper is received, it will be sent to the reviewers for feedback and evaluation. The article will be accepted, returned for revision or rejected. All these process are managed through the online system to expedite the review and publication process. \u0000As a publication of a research article concludes the research process, we invite scholars and researchers to submit original research articles, reviews, commentaries and case studies. Our experts review board panel will, and the editorial board will be available to answer any queries you have. Additionally, feel free to explore the webpage of the journal to know about the submission process, requirement for publication and get updates on your submission.","PeriodicalId":359742,"journal":{"name":"International Journal of Medicine and Biomedical Sciences","volume":"4 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132160024","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prospective Study on Bacterial Isolates with their Antibiotic Susceptibility Pattern from Pus (Wound) Sample in Kathmandu Model Hospital","authors":"P. Chaudhary, Chetan Shakya, S. Pokhrel, Sushil Karki, B. Shrestha, B. Timalsina","doi":"10.55530/ijmbiosnepal.v1i1.5","DOIUrl":"https://doi.org/10.55530/ijmbiosnepal.v1i1.5","url":null,"abstract":"\u0000Background: Wound infection is a very common infection throughout the world and is causing a great fear in developing countries like Nepal. The present study was carried out in Kathmandu Model Hospital with an aim to find out the bacteriological episodes in pus samples in relation to age and sex, Antibiotic Susceptibility Testing (AST) and drug resistance pattern among the isolates. \u0000Methodology: A total of 157 pus samples included in the study were processed in the Microbiology laboratory of Kathmandu Model Hospital using standard microbiological techniques. Identification of organisms was done on the basis of microscopy, colony morphology, and biochemical tests. The antibiotic sensitivity testing of all isolates was performed by Kirby Bauer’s disc diffusion method on Muller Hinton agar and interpreted as per CLSI guidelines. \u0000Results: Analysis of 157 pus samples showed (93, 59.24%) culture positive cases with (63, 67.74%) Multi Drug Resistance (MDR) isolates. Age group (21-30) was found to be the most vulnerable age group. Staphylococcus aureus (29, 28.71%) was the predominant organism isolated among gram positive bacteria and Escherichia coli (26, 25.74%) among gram negative bacteria. Gentamycin was the most sensitive antibiotic among gram positive bacteria whereas Amikacin being the most sensitive Antibiotic among gram negative bacteria. \u0000Conclusion: Antibiograms of isolates can serve as a tool for physicians to start an empirical treatment and minimize the drug resistance problem. \u0000","PeriodicalId":359742,"journal":{"name":"International Journal of Medicine and Biomedical Sciences","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130854273","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Initiation of IJMBioS","authors":"S. Gupte","doi":"10.55530/ijmbiosnepal.v1i1.2","DOIUrl":"https://doi.org/10.55530/ijmbiosnepal.v1i1.2","url":null,"abstract":"\u0000It gives me a great pleasure to be a small contributor to the first inaugural issue of “International Journal of Medicine & Biomedical Sciences” being published from Nepal with efforts put in by Nepalese Researches. As the name suggests, this journal is mainly devoted to Biomedical Sciences & Medical sciences and related topics. On this special auspicious occasion I would like to take opportunity to share some of my thoughts.\u0000The medical microbiology revolves around three main areas and topics such as type's infectious diseases, diagnosis methods andtreatment options.  Over the past decades, major advances in the field of molecular biology, coupled with advances in multiplex real time PCR technologies have facilitated the development of high performing,innovative, low cost and syndrome based tests for the diagnosis of various bacteria, viruses and parasites  with the aid of   fast track diagnostics.  These technologies are particularly specialised in symptoms based disease approach as different pathogens can cause similar clinical pictures while diagnosed for  respiratory infections, gastroenteritis, sexually transmitted infections, fever, rash, childhood infections, hepatitis, meningitis, infections of the immune suppressed, tropical fever and many other infections. Fungal infections are increasing worldwide specially with increase in immune compromised patients. Correct and accurate laboratory diagnosis of fungal infections has become an essential part of the laboratory services.\u0000 \u0000After achieving success in diagnosis,it's now a turn of a clinician to give most appropriate antimicrobial treatment from the available armament of antimicrobial agents.  At this point the gloomy scenario surfaces. Due to ever increasing number of resistant organisms treatment options for clinicians are very limited. Emergence of MRSA, VRSA, ESBL and MBL including NDM1 and so on have made one time considered most powerful antibiotics blunt in action. The number of strains resistant to different antibiotics and their ever increasing number revealed in several publications worldwide are really scary. The situation is really bad in Asian countries. Lot of discussion goes and finally blame points at uncontrolled misuse of antibiotics. Though it is true, there are certain associated socio-economic issues that need to be considered. The  clinician is often forced to  go for empirical  antibiotic treatment due to high cost of susceptibility tests needed to be performed before starting the appropriate antibiotic treatment and moreover even if he wants,  it not easily  available particularly in rural areas. \u0000Additionally, leading international Pharma companies have either stopped their search for new antibiotics or slowed down   and instead prefer to invest in lifestyle  diseases  such as high blood pressure, diabetes and asthma due to their higher shelf life, unlike that of  antibiotics. The    safety issues and very high cost of clinical trials coupled with uncertainty of ","PeriodicalId":359742,"journal":{"name":"International Journal of Medicine and Biomedical Sciences","volume":"43 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2015-10-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116016846","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}