The Maillard Reaction in Foods and Medicine最新文献_第2页

Endocytic uptake of AGE-modified low-density lipoprotein by macrophages leads to cholesteryl ester accumulation in vitro 巨噬细胞内吞摄取age修饰的低密度脂蛋白导致胆固醇酯在体外积累

The Maillard Reaction in Foods and Medicine Pub Date : 1900-01-01 DOI: 10.1533/9781845698447.7.391

Y. Jinnouchi, T. Higashi, K. Ikeda, H. Sano, R. Nagai, H. Hakamata, Masaki Yoshida, Shoichi Ucda, S. Horiuchi

{"title":"Endocytic uptake of AGE-modified low-density lipoprotein by macrophages leads to cholesteryl ester accumulation in vitro","authors":"Y. Jinnouchi, T. Higashi, K. Ikeda, H. Sano, R. Nagai, H. Hakamata, Masaki Yoshida, Shoichi Ucda, S. Horiuchi","doi":"10.1533/9781845698447.7.391","DOIUrl":"https://doi.org/10.1533/9781845698447.7.391","url":null,"abstract":"Recent studies disclosed that proteins modified by advanced glycation endproducts (AGE) are taken up by macrophages or macrophage-derived cells by the macrophage scavenger receptor (MSR) in vitro and that AGE-proteins are accumulated in foam cells in the human atherosclerotic lesions in vivo , suggesting a possibility that AGE-modified low density lipoprotein (AGE-LDL) in situ might be involved in atherogenic processes in vivo . As a first step, AGE-LDL was prepared by incubating LDL with glycolaldehyde (GA), a highly reactive intermediate of the Maillard reaction. GA-modified LDL (GA-LDL) is characterized by an increase in negative charge, fluorescent intensity and reactivities to anti-AGE antibodies, suggesting that these physicochemical and immunochemical properties of GA-LDL were highly similar to those of AGE-proteins. Furthermore, studies of cellular interaction of GA-LDL with mouse peritoneal macrophages showed that GA-LDL is recognized and endocytosed, followed by intralysosomal degradation by these cells. Endocytic uptake of GA-LDL by these cells was competitively inhibited by acetylated LDL (acetyl-LDL), a representative ligand for MSR. Endocytic degradation of 125 I-acetyl-LDL was competed for by GA-LDL. Furthermore, incubation of GA-LDL effectively converted them into foam cells. Similar results were obtained from CHO cells overexpressing MSR. These results suggest that LDL modified by AGE in situ is taken up by macrophages mainly via MSR and contributed to foam cell formation in the early atherosclerotic lesions.","PeriodicalId":359473,"journal":{"name":"The Maillard Reaction in Foods and Medicine","volume":"83 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125421341","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Commitment of the European Commission to Finance: “Optimisation of the Maillard Reaction - A Way to Improve Quality and Safety of Thermally Processed Foods” 欧盟委员会对资助的承诺:“优化美拉德反应——提高热加工食品质量和安全的一种方式”

The Maillard Reaction in Foods and Medicine Pub Date : 1900-01-01 DOI: 10.1533/9781845698447.8.401A

A. Arnoldi

引用次数: 0

Pro-Inflammatory Cytokine Synthesis by Human Monocytes Induced by Proteins Minimally-Modified by Methylglyoxal 甲基乙二醛微修饰蛋白诱导单核细胞合成促炎性细胞因子

The Maillard Reaction in Foods and Medicine Pub Date : 1900-01-01 DOI: 10.1533/9781845698447.7.357

E. A. Abordo, Paul J Thornalley

{"title":"Pro-Inflammatory Cytokine Synthesis by Human Monocytes Induced by Proteins Minimally-Modified by Methylglyoxal","authors":"E. A. Abordo, Paul J Thornalley","doi":"10.1533/9781845698447.7.357","DOIUrl":"https://doi.org/10.1533/9781845698447.7.357","url":null,"abstract":"Methylglyoxal is a reactive α-oxoaldehyde, physiological metabolite and potent glycating agent. It reacts with lysine and arginine residues irreversibly to form advanced glycation endproducts (AGE): N δ -(1-carboxyethyl)lysine, 4-methylimidazolium crosslinks, hydroimidazolone, N δ -(5-hydro-5-methyl-4-imidazolon-2-yl)ornithine and imidazolone N δ -(5-methyl-4-imidazolon-2-yl)ornithine residues. The induction of pro-inflammatory cytokines by AGE-modified protons has been suggested as a mechanism by which AGE accumulation in vivo may contribute to the development of diabetic complications and other disease mechanisms. Proteins typically have only minimal modification by AGE (1-3 AGE per protein molecule) in vivo , even in pathological states. We therefore studied the induction of cytokines in human monocytes in vitro by minimally advanced glycated proteins. Human serum albumin minimally modified by methylglyoxal stimulated the synthesis and secretion of interleukin-1β tumour necrosis factor-α and macrophage-colony stimulating factor. Human serum albumin minimally- or highly-modified by glucose-derived AGE gave a much weaker response under the same conditions. This suggests that the modification of proteins by methylglyoxal produced a potent pharmacophore for activation of the cytokine response. Cytokine induction in vivo provides a mechanism by which the accumulation of AGE, particularly methylglyoxal-modified proteins, may contribute to the development of diabetic complications, chronic renal insufficiency, Alzheimer's disease and aging.","PeriodicalId":359473,"journal":{"name":"The Maillard Reaction in Foods and Medicine","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129388963","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Cross-Linking of Proteins by Maillard Processes: Model Reaction of an Amadori Compound with Nα-Acetyl-L-Arginine 美拉德过程中蛋白质的交联:Amadori化合物与n - α-乙酰- l-精氨酸的模型反应

The Maillard Reaction in Foods and Medicine Pub Date : 1900-01-01 DOI: 10.1533/9781845698447.8.409

F. Gerum, T. Severin

引用次数: 1

Cytotoxicity of Advanced Glycation Endproducts 晚期糖基化终产物的细胞毒性

The Maillard Reaction in Foods and Medicine Pub Date : 1900-01-01 DOI: 10.1533/9781845698447.8.430

G. Münch, Birgit Geiger, C. Loske, A. Simm, R. Schinzel

引用次数: 0

The Maillard Reaction in the Formation of Brown Pigments in Preserves 蜜饯中棕色色素形成中的美拉德反应

The Maillard Reaction in Foods and Medicine Pub Date : 1900-01-01 DOI: 10.1533/9781845698447.8.406A

W. El-Gendy, Hanaa Ismail, E. Salem

引用次数: 0

Macrophage Scavenger Receptor Mediates The Endocytic Uptake of Advanced Glycation End Products (AGEs) 巨噬细胞清道夫受体介导晚期糖基化终产物(AGEs)的内吞摄取

The Maillard Reaction in Foods and Medicine Pub Date : 1900-01-01 DOI: 10.1533/9781845698447.7.380

S. Horiuchi, T. Higashi, H. Sano, Kenshi Matsumaoto, R. Nagai, Hiroshi Suzuki, T. Kodama, M. Shichiri

{"title":"Macrophage Scavenger Receptor Mediates The Endocytic Uptake of Advanced Glycation End Products (AGEs)","authors":"S. Horiuchi, T. Higashi, H. Sano, Kenshi Matsumaoto, R. Nagai, Hiroshi Suzuki, T. Kodama, M. Shichiri","doi":"10.1533/9781845698447.7.380","DOIUrl":"https://doi.org/10.1533/9781845698447.7.380","url":null,"abstract":"Summary Cellular interactions of AGEs are mediated by AGE receptors. The AGE receptors so far reported are RAGE, galectin-3 and MSR (macrophage scavenger receptor). Macrophages or macrophage-derived cells are known to show the highest endocytic activity for AGE-proteins. Our recent study using CHO (Chinese Hamster Ovary) cells overexpressing MSR clearly showed that the endocytic uptake of AGE-proteins by macrophages is mediated by MSR. To strengthen this contention, the present study was undertaken to examine the interaction of AGE-proteins with peritoneal macrophages from MSR-deficient mice (MSR (-/-)). In experiments at 37°C, thioglycolate-induced peritoneal macrophages from MSR (-/-) showed a marked decrease (more than 80%) in the endocytic degradation capacity for 125 I-acetylated low-density lipoprotein (acetyl-LDL). Under parallel conditions, the degradation activity of 125 I-AGE-bovine serum albumin (BSA) by these MSR-deficient macrophages was less than 20%. The remaining endocytic capacity of 125 I-AGE-BSA by these MSR-deficient macrophages was not inhibited by acetyl-LDL, but was inhibited significantly by AGE-BSA, AGE-hemoglobin or polyanions such as dextran sulfate and polyinosinic acid. These results indicate that −80% of the endocytic uptake of AGE-proteins by macrophages is mediated by MSR, while the remaining part is mediated by other AGE receptors.","PeriodicalId":359473,"journal":{"name":"The Maillard Reaction in Foods and Medicine","volume":"19 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124096760","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Browning Reactions of Dehydroascorbic acid with Aspartame in Aqueous Solutions and Beverages 脱氢抗坏血酸与阿斯巴甜在水溶液和饮料中的褐变反应

The Maillard Reaction in Foods and Medicine Pub Date : 1900-01-01 DOI: 10.1533/9781845698447.8.442

H. Sakurai, K. Ishii

引用次数: 0

Recent Advances in the Analysis of Coloured Maillard Reaction Products 有色美拉德反应产物分析的新进展

The Maillard Reaction in Foods and Medicine Pub Date : 1900-01-01 DOI: 10.1533/9781845698447.2.76

J. Ames, R. G. Bailey, J. Mann

引用次数: 1

Studies of the Mutagenic Action of Maillard Reaction Products from Triose Reductone and Amino Acids 三糖还原素与氨基酸美拉德反应产物的致突变性研究

The Maillard Reaction in Foods and Medicine Pub Date : 1900-01-01 DOI: 10.1533/9781845698447.8.449A

Y. Tseng

引用次数: 0