Turk hijiyen ve deneysel biyoloji dergisi. Turkish bulletin of hygiene and experimental biology最新文献_第7页

The antiproliferative and cytotoxic effects of curcumin on human cervical cancer Hep2C cell line 姜黄素对人宫颈癌Hep2C细胞系的抗增殖和细胞毒作用

Turk hijiyen ve deneysel biyoloji dergisi. Turkish bulletin of hygiene and experimental biology Pub Date : 2022-01-01 DOI: 10.5505/turkhijyen.2022.47154

F. Alanyalı, M. Alkan

{"title":"The antiproliferative and cytotoxic effects of curcumin on human cervical cancer Hep2C cell line","authors":"F. Alanyalı, M. Alkan","doi":"10.5505/turkhijyen.2022.47154","DOIUrl":"https://doi.org/10.5505/turkhijyen.2022.47154","url":null,"abstract":"Objective: In this study, antiproliferative and cytotoxic effects of different concentrations of curcumin on cervical cancer Hep2C cells were investigated with microscopic methods and MTT assay. Methods: Hep2C (human carcinoma cancer cell line, ATCC:CCL-23) cells were cultured. For cytotoxicity evaluation Hep2C cells exposed to curcumin at different concentrations of 30 µg/ml, 15 µg/ml, 7.5 µg/ml, 3.7 µg/ml, 1.9 µg/ml, 0.9 µg/ml, 0.45 µg/ml for 24 hours These Hep2C cells are evaluated with MTT assay. The IC 50 value of the agent for 24 h of exposure was detected. The graph of the absorbance data obtained by the Spectramax I3 device. Viability values of Hep2C cells calculated from the absorbances obtained from MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay are gained. The preparations were observed based on changes in nuclei and structures using an inverted microscope (Leica Microsystems). Nontreated cells were used as negative control and for positive control Hep2C cells were exposed to ammonium molibdate (1mg/ml) for the above given incubation period. hücre kültürü Results: High doses of curcumin µg/ml, 15 µg/ml, 7.5 µg/ml) showed high antiproliferative and cytotoxic effects on Hep2C cells. The antiproliferative and cytotoxic effects were not observed on cervical cancer Hep2C cells treated with lower concentrations of curcumin. Conclusion: Curcumin has been shown that it is non-toxic, can be used as a highly antioxidant and anti-inflammatory agent and has multifaced therapeutic-pharmacological effects. However, researches on the antiproliferative, anti-cancer effects of curcumin in cervical cancer cells is not sufficient. The present study evaluates the antiproliferative and cytotoxic effects of curcumin on human cervical cancer Hep2C cells as the first time. The results of our study support these effects of curcumin on Hep2C cells in a concentration-dependent","PeriodicalId":35553,"journal":{"name":"Turk hijiyen ve deneysel biyoloji dergisi. Turkish bulletin of hygiene and experimental biology","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70676216","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigation of Legionella pneumophila and other Legionella species in atypical pneumonia patients 非典型肺炎患者嗜肺军团菌及其他军团菌的调查

Turk hijiyen ve deneysel biyoloji dergisi. Turkish bulletin of hygiene and experimental biology Pub Date : 2022-01-01 DOI: 10.5505/turkhijyen.2022.45722

Kerim Parlak, A. Gözalan, S. Aydoğan, A. Koyuncu, H. Hasanoğlu, Selin Nar Ötgün, Z. C. Açıkgöz

引用次数: 0

In vitro efficacy and field effectiveness of silica-based acaricide against Dermanyssus gallinae 二氧化硅基杀螨剂对鸡皮螨的体外药效和田间药效

Turk hijiyen ve deneysel biyoloji dergisi. Turkish bulletin of hygiene and experimental biology Pub Date : 2022-01-01 DOI: 10.5505/turkhijyen.2022.43799

Nafiye Koç, S. Nalbantoğlu

引用次数: 0

Evaluation of the performance of LED fluorescent microscopy in diagnosis of tuberculosis LED荧光显微镜在肺结核诊断中的应用价值评价

Turk hijiyen ve deneysel biyoloji dergisi. Turkish bulletin of hygiene and experimental biology Pub Date : 2022-01-01 DOI: 10.5505/turkhijyen.2022.46244

Gizem Erdal, S. Sürücüoğlu, N. Özkütük

引用次数: 0

Evaluation of Intestinal Parasites and Risk Factors in Patients Applying to Outpatient Clinics with Digestive System Complaints in Ordu province 奥尔都省消化系统疾患门诊患者肠道寄生虫及其危险因素评价

Turk hijiyen ve deneysel biyoloji dergisi. Turkish bulletin of hygiene and experimental biology Pub Date : 2022-01-01 DOI: 10.5505/turkhijyen.2022.63549

Ülkü Karaman, Y. Kaya, Ö. Özdemir, Ö. Enginyurt, Z. Gamsızkan, C. Colak, G. Yolalan, Ş. Top, Merve Bi̇ngöl

{"title":"Evaluation of Intestinal Parasites and Risk Factors in Patients Applying to Outpatient Clinics with Digestive System Complaints in Ordu province","authors":"Ülkü Karaman, Y. Kaya, Ö. Özdemir, Ö. Enginyurt, Z. Gamsızkan, C. Colak, G. Yolalan, Ş. Top, Merve Bi̇ngöl","doi":"10.5505/turkhijyen.2022.63549","DOIUrl":"https://doi.org/10.5505/turkhijyen.2022.63549","url":null,"abstract":"Objective: The aim of this study is to investigate the parasite prevalence, species and factors affecting parasite positivity in patients with digestive system complaints in Ordu Province in Turkey. Methods: 317 patients who presented with gastrointestinal complaints were included in the study. A questionnaire including; socio-demographic characteristics of the patients, lifestyle and digestive system complaints was applied. Cellophane band method, nativ-lugol, sedimentation and Modifiye kinyoun acid-fast methods were used for the diagnosis of fecal parasites. After the samples were prepared and stained, they were examined under a microscope. Results: Parasite positivity was detected in 205 (64.66%) of 317 patients. Blastocystis spp. constitutes 34.1%, 34.7% of Cryptosporidium spp and 16.1% of Entamoeba coli . The incidence of helminths was 2.8% and the incidence of protozoa was 97.2%. 73.7% of using city water, 93.7% were eating vegetables predominantly. In 9 (4.4%) of the patients with parasite positivity, no parasite was detected at the first examination, and it was detected in the 2nd and 3rd Bakers. The complaints of salivation in Entamoeba coli , Blastocystis spp. and Cryptosporidium spp, and allergy, rectal itching, drooling, increased appetite were significantly higher in Enterobius vermicularis ( p <0.05). Conclusion: It has been observed that there is a high rate of parasites in patients presenting with digestive complaints. Mostly Blastocystis spp., Cryptosporidium spp species were observed in Ordu Province. Patients who present with the complaint of digestion, especially those whose complaints do not improve, should definitely be evaluated in terms of parasites and the preliminary diagnosis of parasitosis should not be immediately ruled out, even if the first stool examination is negative.","PeriodicalId":35553,"journal":{"name":"Turk hijiyen ve deneysel biyoloji dergisi. Turkish bulletin of hygiene and experimental biology","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70676784","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigation of The Antibacterial and Anticarcinogenic Effects of Inula viscosa Methanol and Hexane Extracts 粘菊甲醇和己烷提取物抑菌抗癌作用的研究

Turk hijiyen ve deneysel biyoloji dergisi. Turkish bulletin of hygiene and experimental biology Pub Date : 2022-01-01 DOI: 10.5505/turkhijyen.2022.55798

B. Erdal, Bahar Yilmaz, Bensu Baylan

{"title":"Investigation of The Antibacterial and Anticarcinogenic Effects of Inula viscosa Methanol and Hexane Extracts","authors":"B. Erdal, Bahar Yilmaz, Bensu Baylan","doi":"10.5505/turkhijyen.2022.55798","DOIUrl":"https://doi.org/10.5505/turkhijyen.2022.55798","url":null,"abstract":"Objective: Inula viscosa is a perennial medicinal plant belonging to the Asteraceae family. This study aimed at investigating the antibacterial and anticarcinogenic activities of methanol and hexane extracts derived from I. viscosa . Methods: The antibacterial activity of different concentrations of I. viscosa methanol (1.56-800 mg/ml) and hexane (0.19-100 mg/ml) extracts was tested using the disc diffusion method. Listeria the hexane extract were 400 mg/ml for L. monocytogenes , E. coli ve K. pneumoniae , and 100 mg/ml for M. luteus . MBC values were similar to MIC values. IC50 values for MDA-MB-231 and HT-29 cells were 25 mg/ml and 200 mg/ ml for the methanol extract, and 6.25 mg/ml and 12.5 mg/ml for the hexane extract, respectively. Depending on increased concentration levels, the presence of necrotic and early apoptotic cells was observed among MDA-MB-231 and HT-29 cells. Conclusion: Methanol and hexane extracts of I. viscosa were found to have antibacterial effects on the studied bacteria and anticarcinogenic activity on MDA-MB-231 and HT-29 cells. Conducting further studies to demonstrate the effects of these extracts on different cancer cell lines and bacterial strains will contribute to the field of","PeriodicalId":35553,"journal":{"name":"Turk hijiyen ve deneysel biyoloji dergisi. Turkish bulletin of hygiene and experimental biology","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70676901","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of Clostridium difficile Toxin B Results by Polymerase Chain Reaction from Stool Specimens 粪便标本中艰难梭菌毒素B聚合酶链反应检测结果的评价

Turk hijiyen ve deneysel biyoloji dergisi. Turkish bulletin of hygiene and experimental biology Pub Date : 2022-01-01 DOI: 10.5505/turkhijyen.2022.77785

Pınar Şamlıoğlu, A. Bayram, Güliz Doğan

{"title":"Evaluation of Clostridium difficile Toxin B Results by Polymerase Chain Reaction from Stool Specimens","authors":"Pınar Şamlıoğlu, A. Bayram, Güliz Doğan","doi":"10.5505/turkhijyen.2022.77785","DOIUrl":"https://doi.org/10.5505/turkhijyen.2022.77785","url":null,"abstract":"Objective: Clostridium difficile is a Gram-positive, anaerobic, spore-forming bacterium that spreads via the fecal-oral route and causes asymptomatic carriage or mild diarrheal diseases such as pseudomembranous enterocolitis. C. difficile is the commonest cause of nosocomial and antibiotic-associated diarrhea in North America and Europe. It can produce two toxins called A and B. Strains producing toxins can lead to a more serious disease picture that causes megacolon, perforation or septic shock. The use of molecular methods with high specificity and SONUÇLARININ DEĞERLENDİRİLMESİ method. The data obtained are interpreted as positive, negative or invalid results. Results: A total of 109 samples were tested. 66 (61%) of the stool samples belonged to male and 43 (39%) female patients. Twenty-five (23%) of the patients were under the age of 18 and 36 (33%) under the age of 18 years. Of the samples, 26 (24%) were from intensive care units, 60 (55%) were from services and 23 (21%) were from polyclinics. It was found 109 from stool samples. 9 (8%), C. difficile PCR test toxin B positive, 100 (92%) negative. Conclusion: The most important cause of nosocomial diarrhea is C. difficile . The main predisposing factor of infection is the use of antibiotics. Prophylaxis or even one dose of antibiotic therapy may be sufficient for the development of C. difficile infection. Rapid and accurate diagnosis by PCR is important for early initiation yaş","PeriodicalId":35553,"journal":{"name":"Turk hijiyen ve deneysel biyoloji dergisi. Turkish bulletin of hygiene and experimental biology","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70677272","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Oxytocin administration improves DNA damage and total oxidative stress parameters in vincristine and cisplatin-induced cortical neuron toxicity 催产素可改善长春新碱和顺铂诱导的皮质神经元毒性的DNA损伤和总氧化应激参数

Turk hijiyen ve deneysel biyoloji dergisi. Turkish bulletin of hygiene and experimental biology Pub Date : 2022-01-01 DOI: 10.5505/turkhijyen.2022.70846

B. Çiçek, A. Taghizadehghalehjoughi, S. Yıldırım, G. Eser, M. Gül, M. Kantarcı, A. Hacımüftüoğlu

引用次数: 0

Parasites of the oral cavity 口腔寄生虫口腔中的寄生虫

Turk hijiyen ve deneysel biyoloji dergisi. Turkish bulletin of hygiene and experimental biology Pub Date : 2022-01-01 DOI: 10.5505/turkhijyen.2022.71473

B. Karasu, Özcan Özkan, Ayşegül Taylan Özkan

引用次数: 0

Association of KIR2DL4 Gene Polymorphisms With Obesity KIR2DL4基因多态性与肥胖的关系

Turk hijiyen ve deneysel biyoloji dergisi. Turkish bulletin of hygiene and experimental biology Pub Date : 2022-01-01 DOI: 10.5505/turkhijyen.2022.95776

Huda Ahmed, Deniz Mihcioglu, Filiz Özbaş Gerçeker, B. Aksoy

引用次数: 0