Acta Microbiologica Bulgarica最新文献_第3页

Antifungal Susceptibility Pattern of Malassezia sp. Isolated from the North-East Region of India 印度东北地区马拉色菌抗真菌药敏型分析

Acta Microbiologica Bulgarica Pub Date : 2023-06-01 DOI: 10.59393/amb23390213

E.K. Marak, Th.N. Singh, Kh.S. Devi, Y.R.B. Singh, Kh.R. Devi

引用次数: 0

Antifungal Activities of Sida acuta Burm. f. Leaf Extracts on the Fruit Rot Pathogen of Annona muricata L. 刺荆芥的抗真菌活性研究。f.番荔枝果腐病菌的叶提取物研究。

Acta Microbiologica Bulgarica Pub Date : 2023-06-01 DOI: 10.59393/amb23390212

D.A. Ehwarieme, O.O. Whiliki

{"title":"Antifungal Activities of Sida acuta Burm. f. Leaf Extracts on the Fruit Rot Pathogen of Annona muricata L.","authors":"D.A. Ehwarieme, O.O. Whiliki","doi":"10.59393/amb23390212","DOIUrl":"https://doi.org/10.59393/amb23390212","url":null,"abstract":"This study investigated the antifungal activities of Sida acuta Burm. f. leaf extracts against fruit rot fungi of Annona muricata (sour sop) and the phytochemical constituent of the plant. Fruits of sour sop (A. muricata) and leaves of Sida acuta used for the study were collected from different locations within Abraka community, Delta State. Qualitative and quantitative phytochemical screening and Gas Chromatography – Mass Spectrometry analysis were carried out on the extract. Fungi causing rot of A. muricata was isolated and antifungal activities of S. acuta on isolated fungi was carried out using the poisoned food method. The result showed that both methanol and chloroform extracts of the plant contained flavonoid, tannin, saponin, steroid, glycoside, alkaloid, anthraquinone, terpenoid, and reducing sugar. The GC-MS result revealed the presence of 20 compounds with 3-Trifluoromethylbenzylamine, N,N-diundecyl and L-Proline, N-(2-trif¬luoromethylbenzoyl)-, isohexyl ester as the most abundant in both methanolic and chloroform extracts. The study identified Rhizopus stolonifer as the fungi associated with the rot of A. muricata and showed that as the concentration of the extract increased, its antimicrobial activity was significantly higher in the organ¬isms. The phytochemical composition and antifungal effects make S. acuta leaf extract a potential agent for use against fungal infections in the future.","PeriodicalId":35526,"journal":{"name":"Acta Microbiologica Bulgarica","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135144659","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Virulence Factors and Antifungal Resistance Pattern of Candida Species Isolated from Patients with Type Two Diabetes 2型糖尿病患者假丝酵母菌的毒力因子及耐药模式

Acta Microbiologica Bulgarica Pub Date : 2023-06-01 DOI: 10.59393/amb23390210

O.M. David, T.O. Faloye, S.A. Dada, P.O. Adeola, J.B. Owabumoye, O. Famurewa

{"title":"Virulence Factors and Antifungal Resistance Pattern of Candida Species Isolated from Patients with Type Two Diabetes","authors":"O.M. David, T.O. Faloye, S.A. Dada, P.O. Adeola, J.B. Owabumoye, O. Famurewa","doi":"10.59393/amb23390210","DOIUrl":"https://doi.org/10.59393/amb23390210","url":null,"abstract":"Diabetes provides an underlying basis for the inability of the body to control infection. Candida albi¬cans has been reported to colonize and cause severe infections in patients with diabetes. This study aimed to evaluate the presence of virulence and antifungal resistance among C. albicans isolates from subjects with type 2 diabetes. Two hundred forty-five and one hundred non-repeat samples were collected from diabetic and non-diabetic subjects, respectively. Cultural and molecular methods were used to confirm the identity of C. albicans isolated from the urine, saliva, and wound samples of the subjects. The presence of virulence factors among the isolated C. albicans and their susceptibility to antifungals were detected using standard microbiological methods. Of the 345 subjects screened in this study, 123 (35.65%) were male, and 222 (64.35%) were female. The prevalence of Candida species was highest among subjects within the 31- 59 years age group. Candida species were recovered from 149 and 49 diabetic and non-diabetic patients, respectively. Apart from C. albicans [n=112 (56.57%)], C. glabrata (n=46 [23.23%]), C. tropicalis [n=13 (6.57%)], and C. krusei [n=5 (2.53%)] also recovered from the subjects. In this study, non-albicans Can¬dida had a lower percentage of virulence factors than C. albicans. Most of the isolates were susceptible to fluconazole and nystatin. There was a correlation between diabetes and C. albicans infections. Candida albicans from diabetic patients were more resistant to the tested antifungals than those from non-diabetic subjects.","PeriodicalId":35526,"journal":{"name":"Acta Microbiologica Bulgarica","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135144765","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Natural and Modified Zeolite Clinoptilolite with Antimicrobial Properties: a Review 天然沸石及改性沸石抗菌性能研究进展

Acta Microbiologica Bulgarica Pub Date : 2023-06-01 DOI: 10.59393/amb23390207

A. Nikolov, L. Dobreva, S. Danova, J. Miteva-Staleva, E. Krumova, V. Rashev, N. Vilhelmova-Ilieva

引用次数: 1

The Immunology of Malaria: А Review 疟疾免疫学:А综述

Acta Microbiologica Bulgarica Pub Date : 2023-06-01 DOI: 10.59393/amb23390205

I.J. Falade, O.N. Simeon

{"title":"The Immunology of Malaria: А Review","authors":"I.J. Falade, O.N. Simeon","doi":"10.59393/amb23390205","DOIUrl":"https://doi.org/10.59393/amb23390205","url":null,"abstract":"Plasmodium falciparum malaria is still very much regarded as a global health challenge recording morbidity close to half a million annually. Special attention has been given to Plasmodium due to this high rate of morbidity. The steps taken towards control have produced a considerable result. Some of these steps are aimed at controlling the vector hosting P. falciparum or a nib in the bud during the several stages of P. falciparum invasion in humans. These controls include effective antimalarials, the use of insecticide-treated nets (ITNs), indoor residual spraying (IRS) campaign, and vaccine. Several vaccines have been tested but none came close in success to the recent vaccine (RTS, S/AS01) which was approved for pilot implementa¬tion program in three African countries in 2015. However, despite the success recorded, this vaccine has its limitations, which include low efficacy in certain age groups, poor immunogenicity, and the need for sev¬eral booster doses to achieve reasonable effectiveness. With this in mind, an intentional search for a more effective vaccine is still ongoing and an understanding of naturally acquired immune responses would help in vaccine design. This review put together what is currently known about P. falciparum and the immune responses against malaria.","PeriodicalId":35526,"journal":{"name":"Acta Microbiologica Bulgarica","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135145297","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Current Status of COVID-19-Associated Secondary Bacterial and Fungal Infection: A Mini Review 2019冠状病毒病相关继发性细菌和真菌感染现状综述

Acta Microbiologica Bulgarica Pub Date : 2023-06-01 DOI: 10.59393/amb23390201

D. Jayakumar, P. Kumar, M. Mini, S. Khan

引用次数: 0

Yersinia enterocolitica - Isolation, Pathogenicity, and Prevalence in Farms for Slaughtered Pigs 屠宰猪小肠结肠炎耶尔森菌的分离、致病性和流行

Acta Microbiologica Bulgarica Pub Date : 2023-06-01 DOI: 10.59393/amb23390204

M. Angelovska, M.M. Zaharieva, H. Najdenski

{"title":"Yersinia enterocolitica - Isolation, Pathogenicity, and Prevalence in Farms for Slaughtered Pigs","authors":"M. Angelovska, M.M. Zaharieva, H. Najdenski","doi":"10.59393/amb23390204","DOIUrl":"https://doi.org/10.59393/amb23390204","url":null,"abstract":"Yersinia enterocolitica is a significant zoonotic pathogen that poses a considerable threat to both animal and human health. This review paper aims to summarize and critically evaluate current knowledge on the isolation, pathogenicity, and prevalence of Y. enterocolitica in farms dedicated to the production of slaughtered pigs. Isolation of Y. enterocolitica from various sources, including porcine tonsils, feces, and mesenteric lymph nodes, is essential for understanding prevalence and transmission dynamics. The different isolation methods and their advantages and limitations in detecting the pathogen are explored, highlighting the challenges associated with its detection. We examine the role of specific virulence factors, in the ability of Y. enterocolitica to colonize and cause disease in humans. Additionally, this review provides a compre¬hensive analysis of factors influencing the prevalence of Y. enterocolitica in farms for slaughtered pigs. The review highlights the need for standardized surveillance strategies to accurately assess the prevalence of Y. enterocolitica in swine populations, ensuring effective control and prevention measures. In conclusion, the findings presented here underscore the importance of Y. enterocolitica as an emergent pathogen as well as implementing comprehensive surveillance programs, adopting effective control measures, and promoting awareness among stakeholders to mitigate the risk associated with Y. enterocolitica infection in both ani¬mals and humans.","PeriodicalId":35526,"journal":{"name":"Acta Microbiologica Bulgarica","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135144646","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Co-Infection of Coronavirus Disease 2019 and Respiratory Syncytial Virus Infection in a Lung Cancer Patient: A Case Report 2019冠状病毒病合并呼吸道合胞病毒感染1例肺癌患者报告

Acta Microbiologica Bulgarica Pub Date : 2023-06-01 DOI: 10.59393/amb23390214

D. Rezaee, R. Goodarzi, S. Bakhtiari, F.T. Asadi, N. Ansari

引用次数: 0

Evaluation of Antimicrobial Activities of Different Extracts from Phlomis tuberosa, Abutilon fruticosum, and Nepeta transcaucasica 结核、槟榔和外高加索荆芥不同提取物的抑菌活性评价

Acta Microbiologica Bulgarica Pub Date : 2023-06-01 DOI: 10.59393/amb23390211

S. Asnaashari, F. Heshmati-Afshar, N. Amin-Aghdam, P. Asgharian, S. Hallaj-Nezhadi

{"title":"Evaluation of Antimicrobial Activities of Different Extracts from Phlomis tuberosa, Abutilon fruticosum, and Nepeta transcaucasica","authors":"S. Asnaashari, F. Heshmati-Afshar, N. Amin-Aghdam, P. Asgharian, S. Hallaj-Nezhadi","doi":"10.59393/amb23390211","DOIUrl":"https://doi.org/10.59393/amb23390211","url":null,"abstract":"The use of medicinal plants for the natural treatment of infections has received much attention. Pre¬viously, plants of Nepeta, Phlomis, and Abutilon genera showed remarkable antimicrobial activities. We aimed to evaluate the antimicrobial effects of three Iranian plants including Abutilon fruticosum Guill. & Perr., Nepeta transcaucasica Grossh., and Phlomis tuberosa (L.) Moench. Aerial parts of the plants were extracted by the maceration method using petroleum ether, chloroform, ethyl acetate (EtAc), and methanol solvents. The antimicrobial activity of the extracts against seven Gram-positive strains and three Gram-neg-ative strains was studied via the disk diffusion method. Potent extracts were fractionated and diameters of the inhibition zone (DIZ) and Minimum Inhibitory Concentration (MIC) were determined. The chloroform extract of N. transcaucasica and EtAc extract of A. fruticosum were the most active extracts. Staphylococ¬cus aureus, was the most sensitive strain to N. transcaucasica (DIZ=17 mm, MIC=3.125 mg/ml), whereas S. epidermidis (DIZ=15 mm, MIC=1.56 mg/ml), and Bacillus subtilis (DIZ=13mm, MIC=0.39 mg/ml) were the most sensitive bacteria to A. fruticosum. Fractions of 40% and 60% EtAc/n-hexane of chloroform extract of N. transcaucasica and fractions of 40%-100% of EtAc extract of A. fruticusum revealed the potent activities. B. cereus was “very sensitive” to 60%-100% fractions of chloroform extract of N. trans¬caucasica (DIZ=16–19 mm, MIC=12.5 mg/ml). S. pneumoniae was “extremely” (DIZ=20 mm) sensitive to the 60% Ethyl acetate fraction of A. fruticosum and noticeably, their antimicrobial effects were greater than that of the standard antibiotic. The results support some of the traditional applications of the studied plants.","PeriodicalId":35526,"journal":{"name":"Acta Microbiologica Bulgarica","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135144761","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of Loop-Mediated Isothermal Amplification Assay for Detection of Mycoplasma hominis in Vaginal Samples in Comparison with Real Time PCR 环介导等温扩增法检测阴道标本人支原体与实时PCR的比较

Acta Microbiologica Bulgarica Pub Date : 2023-06-01 DOI: 10.59393/amb23390209

H. Hossainpour, S. Bakhtiari, F. Moradi, M.S. Alikhani, R.Y. Mashouf, M.M. Ari, A. Alvandi, M. Karami, S. Rabiee, M.Y. Alikhani

{"title":"Evaluation of Loop-Mediated Isothermal Amplification Assay for Detection of Mycoplasma hominis in Vaginal Samples in Comparison with Real Time PCR","authors":"H. Hossainpour, S. Bakhtiari, F. Moradi, M.S. Alikhani, R.Y. Mashouf, M.M. Ari, A. Alvandi, M. Karami, S. Rabiee, M.Y. Alikhani","doi":"10.59393/amb23390209","DOIUrl":"https://doi.org/10.59393/amb23390209","url":null,"abstract":"Mycoplasma hominis as one of the etiologic agents of non-gonococcal urethritis in women should be identified for early treatment that led to the patient does not suffer from serious complications such as pelvic inflammatory disease and infertility. With regard to the importance of M. hominis in genitourinary diseases, is required for the choice of the appropriate, reliable, simple, and repeatable laboratory assay for diagnosis. The focus of this study was to investigate 16srRNA-based LAMP assay for the identification of M. hominis from vaginal swabs in comparison with quantitative real-time PCR (qPCR). The eighty vaginal swabs were taken and analyzed by cultures, qPCR, and LAMP assay. Totally, 13 (16.3%), 14 (17.5%), and 25 (31.3%) samples were positive for M. hominis by culture, qPCR, and LAMP assay, respectively. The results indicate a sensitivity of 100%, a specificity of 83.3%, a negative predictive value (NPV) of 100%, and a Positive predictive value (PPV) of 56% of the developed LAMP assays. According to the result, the LAMP assay is significantly more specific, and sensitive than both qPCR and culture methods for diagnosis of M. hominis.","PeriodicalId":35526,"journal":{"name":"Acta Microbiologica Bulgarica","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135144764","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0