Acta Biologica Szegediensis最新文献_第5页

Understanding the nature and dynamics of Mycobacterium ulcerans cytochrome P450 monooxygenases (CYPs) – a bioinformatics approach 了解溃疡分枝杆菌细胞色素P450单加氧酶（CYPs）的性质和动力学——一种生物信息学方法

Acta Biologica Szegediensis Pub Date : 2021-08-21 DOI: 10.14232/abs.2021.1.93-103

S. Sur

{"title":"Understanding the nature and dynamics of Mycobacterium ulcerans cytochrome P450 monooxygenases (CYPs) – a bioinformatics approach","authors":"S. Sur","doi":"10.14232/abs.2021.1.93-103","DOIUrl":"https://doi.org/10.14232/abs.2021.1.93-103","url":null,"abstract":"Cytochrome P450 monooxygenases (CYPs or P450s) are catalytically versatile hemoproteins, associated with drug metabolism, substrate utilization and pathogenesis. Mycobacterium ulcerans is a human pathogen causing Buruli ulcer. The study intended to investigate frequency and diversity of CYPs from M. ulcerans strains, understand the pan-CYPome clustering patterns and interconnection of CYPs using bioinformatics tools. M. ulcerans strains demonstrated the presence of 261 CYPs categorized into 35 families and 38 subfamilies. CYP138, CYP140, CYP189 and CYP125 were the ﬂourishing families. Around, 20 CYP families and 20 subfamilies were conserved. Flourishing and conserved CYP families/subfamilies were associated with lipid metabolism, substrate utilization etc. CYP140 had a role in pathogenesis. CYP279 was the least dominant family. CYP135, CYP183, CYP190, CYP271 and CYP276 were diagnostic markers for M. ulcerans subsp. shinshuense strain ATCC 33728 and M. ulcerans strain P7741. The pan-CYPome specified that M. ulcerans is evolving by gaining CYPs. CYP centric clustering revealed diversity and resemblances among M. ulcerans strains. More diverse nature of the M. ulcerans strain Harvey could be attributed to its larger size and geographical location. Co-occurrence network demonstrated mutual associations amongst substantial number of CYP families/subfamilies. This work provided comprehensive understanding of previously unexplored CYPs from M. ulcerans.","PeriodicalId":34918,"journal":{"name":"Acta Biologica Szegediensis","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46473688","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Insights on carbapenem-resistant Pseudomonas aeruginosa 碳青霉烯类耐药铜绿假单胞菌研究进展

Acta Biologica Szegediensis Pub Date : 2021-08-21 DOI: 10.14232/abs.2021.1.105-112

M. Gajdács, K. Kárpáti, Anette Stájer, S. Zanetti, M. Donadu

{"title":"Insights on carbapenem-resistant Pseudomonas aeruginosa","authors":"M. Gajdács, K. Kárpáti, Anette Stájer, S. Zanetti, M. Donadu","doi":"10.14232/abs.2021.1.105-112","DOIUrl":"https://doi.org/10.14232/abs.2021.1.105-112","url":null,"abstract":"Pseudomonas aeruginosa (P. aeruginosa) is ubiquitous in nature, and may be a causative agent in severe, life-threatening infections. In >60% of cases, β-lactam antibiotics are used in the therapy of P. aeruginosa infections, therefore the emergence of carbapenem-resistant P. aeruginosa (CRPA) is a signiﬁcant clinical concern. In this study, phenotypic methods were used to characterize ﬁfty-four (n = 54) P. aeruginosa isolates, which were included based on their suspected non-susceptibility to meropenem. Minimum inhibitory concentrations (MICs) of meropenem, ceftazidime, cefepime, ciprofloxacin, gentamicin, were determined using E-tests, while colistin MICs were determined using broth microdilution. The isolates were subjected to the modiﬁed Hodge test (MHT), the modiﬁed carbapenem-inactivation method (mCIM) and the imipenem/EDTA combined disk test (CDT). AmpC and eﬄux pump overexpression was studied using agar plates containing cloxacillin and phenylalanine-arginine β-naphthylamide (PAβN), respectively. Assessment of bioﬁlm-formation was carried out using the crystal violet tube-adherence method. 38.9% of the strains showed meropenem MICs in the resistant range (>8 mg/L). Eﬄux-pump overexpression and AmpC-hyperproduction was seen in 44.4% and 35.2% of isolates, respectively. 88.8% of the isolates were characterized as strong bioﬁlm-producers. On the other hand, the presence of carbapenemases was suspected in a minority (16.7%) of tested isolates. As safe and eﬀective therapeutic options in carbapenem-resistant Gram-negative infections are severely limited, characterization of these isolates using phenotypic and molecular-based methods is important to provide insights into the epidemiological features of these pathogens.","PeriodicalId":34918,"journal":{"name":"Acta Biologica Szegediensis","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44470056","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

Action of Ganoderma lucidum mycelial growth ﬁltrates on Erysiphe diﬀusa and embryotoxicity assessment in a chicken embryo model 灵芝菌丝生长液对丹毒的作用及鸡胚模型中的胚胎毒性评估

Acta Biologica Szegediensis Pub Date : 2021-08-21 DOI: 10.14232/abs.2021.1.47-57

M. P. D. Cruz, Luca Larentis, Edgar de S. Vismara, L. Vismara, P. F. D. Freitas, S. Mazaro

{"title":"Action of Ganoderma lucidum mycelial growth ﬁltrates on Erysiphe diﬀusa and embryotoxicity assessment in a chicken embryo model","authors":"M. P. D. Cruz, Luca Larentis, Edgar de S. Vismara, L. Vismara, P. F. D. Freitas, S. Mazaro","doi":"10.14232/abs.2021.1.47-57","DOIUrl":"https://doi.org/10.14232/abs.2021.1.47-57","url":null,"abstract":"This work aimed to evaluate the antimicrobial eﬀect of Ganoderma lucidum mycelial growth filtrates (MGF) on the phytopathogen Erysiphe diffusa and their potential eﬀects on the embryonic development of Gallus gallus. The antimicrobial activity was evaluated on E. diffusa spores by the microdilution broth method. To evaluate embryotoxic and teratogenic eﬀects, fertile eggs of G. gallus received injections of solutions containing the filtrates of G. lucidum through the air chamber. After three days of incubation, we opened the eggs and evaluated egg viability, embryo survival, malformation occurrence, embryonic staging and heart rate. Live embryos were prepared using whole mount technique and the morphological analysis was performed. We used the generalized linear model to fit embryotoxicity and teratogenicity data. We verified that G. lucidum MGF showed inhibitory activity in vitro against E. diffusa and the minimum inhibitory concentrations ranged from 5 to 10 mg/mL. We could also observe that the filtrates did not present embryotoxic or teratogenic eﬀects on the early embryonic development of G. gallus, but induced significant diﬀerences in the embryonic mean heart rate and on the stage of embryonic development.","PeriodicalId":34918,"journal":{"name":"Acta Biologica Szegediensis","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41985072","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lipid biomarker-based verification of TB infection in mother’s and daughter’s mummified human remains (Vác Mummy Collection, 18th century, CE, Hungary) 基于脂质生物标志物的母亲和女儿木乃伊遗骸中结核病感染的验证（Vác木乃伊收藏，18世纪，CE，匈牙利）

Acta Biologica Szegediensis Pub Date : 2021-04-10 DOI: 10.14232/ABS.2020.2.99-109

O. Váradi, Ildikó Szikossy, Olga Spekker, D. Rakk, G. Terhes, E. Urbán, William Berthon, I. Pap, F. Maixner, A. Zink, C. Vágvölgyi, H. Donoghue, D. Minnikin, G. Pálfi, A. Szekeres

{"title":"Lipid biomarker-based verification of TB infection in mother’s and daughter’s mummified human remains (Vác Mummy Collection, 18th century, CE, Hungary)","authors":"O. Váradi, Ildikó Szikossy, Olga Spekker, D. Rakk, G. Terhes, E. Urbán, William Berthon, I. Pap, F. Maixner, A. Zink, C. Vágvölgyi, H. Donoghue, D. Minnikin, G. Pálfi, A. Szekeres","doi":"10.14232/ABS.2020.2.99-109","DOIUrl":"https://doi.org/10.14232/ABS.2020.2.99-109","url":null,"abstract":"The perpetual burden of tuberculosis (TB) keeps drawing the focus of research on this disease. Among other risk factors (e.g., poor living conditions, malnutrition, smoking, HIV infection, etc.), being in close contact with a TB infected person requires special attention. For a better understanding of the disease, paleopathological investigations concerning TB have been carried out with various techniques for a long a time; nevertheless, analysis of incidence among family members is hardly possible in past populations. An exceptional group of naturally mummified individuals, the collection of the Vác mummies (Hungary, 18th century CE), is known about the large TB incidence rate, which has been revealed by aDNA analysis. Besides the high rate of TB infection, another interesting aspect of the collection is that in some cases, the family connections could be reconstructed. In this paper, we present the mycocerosic acid profiles gained by HPLC-HESI-MS measurements of two Vác mummies, who were mother and daughter according to the personal records. Earlier metagenomic analysis already revealed mixed M. tuberculosis infection with the same bacterial strains in both individuals; moreover, the same bacterial strains were recorded in both cases.","PeriodicalId":34918,"journal":{"name":"Acta Biologica Szegediensis","volume":"64 1","pages":"99-109"},"PeriodicalIF":0.0,"publicationDate":"2021-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45503813","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Concomitant yield optimization of tannase and gallic acid by Bacillus licheniformis KBR6 through submerged fermentation: An industrial approach 地衣芽孢杆菌KBR6深层发酵产单宁酶和没食子酸的工艺优化

Acta Biologica Szegediensis Pub Date : 2021-04-10 DOI: 10.14232/ABS.2020.2.151-158

P. K. Das Mohapatra, Ishita Biswas, K. C. Mondal, B. Pati

{"title":"Concomitant yield optimization of tannase and gallic acid by Bacillus licheniformis KBR6 through submerged fermentation: An industrial approach","authors":"P. K. Das Mohapatra, Ishita Biswas, K. C. Mondal, B. Pati","doi":"10.14232/ABS.2020.2.151-158","DOIUrl":"https://doi.org/10.14232/ABS.2020.2.151-158","url":null,"abstract":"The present study is concerned with the evaluation of tannase and gallic acid production eﬃcacy of Bacillus licheniformis KBR6 under different environmental conditions through submerged fermentation. Results have shown that different environmental conditions and mineral sources have differential inﬂuences on tannase and gallic acid production. Highest tannase and gallic acid yield was observed at incubation period of 18 h and 22 h, respectively. At tannic acid concentration of 15 g/l, maximum cell mass (0.75 g/l), cell yield coeﬃcient (0.08 g/g), speciﬁc growth rate (37.5 mg/g/h), tannase yield (16.3 U/g) and speciﬁc tannase production rate (0.80 U/g/h) were observed, however, at higher tannic acid concentration a decrease in tannase yield and production rate were observed, but gallic acid production increased with increasing tannic acid concentration. Additional carbohydrate sources like glucose, fructose, and lactose showed positive inﬂuence on enzyme yield. Among the studied nitrogen sources urea and NH4Cl, and of the phosphate sources KH2PO4 showed favourable effects on cell growth and simultaneous enzyme and gallic acid production. Temperature of 35 °C was found to be optimum for tannase and gallic acid production. Of all the studied metal ions Ca2+, Mg2+ and Na+ showed positive effect whereas, Co2+, Ag2+, Pb2+, Hg2+ showed inhibitory effects.","PeriodicalId":34918,"journal":{"name":"Acta Biologica Szegediensis","volume":"64 1","pages":"151-158"},"PeriodicalIF":0.0,"publicationDate":"2021-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44947925","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Perturbation of the mucosa-associated anaerobic gut microbiota in streptozotocin-induced diabetic rats 链脲佐菌素诱导的糖尿病大鼠粘膜相关厌氧肠道微生物群的扰动

Acta Biologica Szegediensis Pub Date : 2021-01-01 DOI: 10.14232/abs.2021.1.75-84

R. Wirth, N. Bódi, Z. Szalai, Lalitha Chandrakumar, G. Maróti, K. Kovács, Z. Bagi, Diána Mezei, János Balázs, M. Bagyánszki

{"title":"Perturbation of the mucosa-associated anaerobic gut microbiota in streptozotocin-induced diabetic rats","authors":"R. Wirth, N. Bódi, Z. Szalai, Lalitha Chandrakumar, G. Maróti, K. Kovács, Z. Bagi, Diána Mezei, János Balázs, M. Bagyánszki","doi":"10.14232/abs.2021.1.75-84","DOIUrl":"https://doi.org/10.14232/abs.2021.1.75-84","url":null,"abstract":"Our aim was to map the gut region-specific diﬀerences of the mucosa-associated microbiome distribution in a streptozotocin-induced diabetic rat model. Tissue samples from the duodenum, ileum and colon were collected 10 weeks after the onset of hyperglycaemia to analyse the mucosa-associated microbiota using next-generation DNA sequencing. Striking diﬀerences were observed in the mucosa-associated microbiota of the duodenum between diabetic and control rats. A significant invasion of the aerobic genus Mycoplasma was apparent in diabetes, and the abundance of the anaerobic phylum Firmicutes decreased massively. It is noteworthy that insulin treatment eliminated the Mycoplasma invasion in the duodenum and apparently restored the anaerobic environment in the mucosa. In the ileum the abundance of the phylum Firmicutes increased in the diabetic samples. Although the proportion of the phylum Proteobacteria decreased moderately, its composition changed significantly, and insulin treatment induced only minor alterations. In the diabetic samples of colon, the abundance of the phylum Firmicutes decreased slightly, the relative number of the bacteria in the phylum Bacteroidetes increased strongly as compared to the control values, and after insulin treatment this increase was more significant. Chronic hyperglycaemia has the most prominent eﬀect on the mucosa-associated microbiota in the duodenum.","PeriodicalId":34918,"journal":{"name":"Acta Biologica Szegediensis","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66816371","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

The effect of type II toxin-antitoxin systems on methicillinresistant Staphylococcus aureus persister cell formation and antibiotic tolerance II型毒素-抗毒素系统对耐甲氧西林金黄色葡萄球菌持久性细胞形成和抗生素耐受性的影响

Acta Biologica Szegediensis Pub Date : 2021-01-01 DOI: 10.14232/abs.2021.1.113-117

Mandana Hosseini, J. Nowroozi, N. Amirmozafari

{"title":"The effect of type II toxin-antitoxin systems on methicillinresistant Staphylococcus aureus persister cell formation and antibiotic tolerance","authors":"Mandana Hosseini, J. Nowroozi, N. Amirmozafari","doi":"10.14232/abs.2021.1.113-117","DOIUrl":"https://doi.org/10.14232/abs.2021.1.113-117","url":null,"abstract":"Persister cells are defi ned as a subpopulation of bacteria in a dormant state with the ability to reduce bacterial metabolism and they are involved in antibiotic tolerance. Toxin-antitoxin (TA) systems have been previously suggested as important players in persistence. Therefore, this study aimed to study the involvement of TA systems in persister cell formation in methicillin-resistant Staphylococcus aureus following antibiotic exposure. Using TADB and RASTA database, two type II TA systems including MazF/MazE and RelE/RelB were predicted in S. aureus. The presence of these TA genes was determined in 5 methicillin-resistant S. aureus isolates and the standard strain S. aureus subsp. aureus N315 using PCR method. To induce persistence, isolates were exposed to lethal doses of ciprofl oxacin and the expression of the studied TA system genes was measured after 5 h using Real-Time PCR. According to our results, all the studied isolates harbored the TA system genes. S. aureus was highly capable of persister cell formation following exposure to sub-MIC of ciprofl oxacin and RT-qPCR showed a signifi cant increase in the expression of the MazEF and RelBE loci, indicating their potential role in antibiotic tolerance. Considering the importance of antibiotic tolerance, further studies on persister cell formation and TA systems involved in this phenomenon are required to effi ciently target these systems.","PeriodicalId":34918,"journal":{"name":"Acta Biologica Szegediensis","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66816105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Acaricidal activity of nishinda (Vitex negundo) leaf and garlic (Allium sativum) bulb extract against red spider mite, Oligonychus coﬀeae (Acari: Tetranychidae) in tea plantations of Darjeeling hill, West Bengal, India 印度西孟加拉邦大吉岭茶园白荆叶和大蒜球茎提取物对红蜘蛛螨的杀螨活性研究

Acta Biologica Szegediensis Pub Date : 2021-01-01 DOI: 10.14232/abs.2021.1.59-64

Piu Banerjee, Arghya Laha, Indrani Samaddar, Himani Biswas, D. Sarkar, Sovan Roy, G. Saha, S. Podder

引用次数: 0

A puriﬁed lectin with larvicidal activity from a woodland mushroom, Agaricus semotus Fr. 从林地蘑菇中提取具有杀幼虫活性的凝集素。

Acta Biologica Szegediensis Pub Date : 2021-01-01 DOI: 10.14232/abs.2021.1.65-73

Isaiah O. Adedoyin, T. Adewole, Titilayo O. Agunbiade, F. Adewoyin, A. Kuku

{"title":"A puriﬁed lectin with larvicidal activity from a woodland mushroom, Agaricus semotus Fr.","authors":"Isaiah O. Adedoyin, T. Adewole, Titilayo O. Agunbiade, F. Adewoyin, A. Kuku","doi":"10.14232/abs.2021.1.65-73","DOIUrl":"https://doi.org/10.14232/abs.2021.1.65-73","url":null,"abstract":"This study investigated the larvicidal activity on Culex quinquefasciatus of lectin purified from fresh fruiting bodies of woodland mushroom, Agaricus semotus. A. semotus lectin (ASL) was purified via ion-exchange chromatography on DEAE-cellulose A-25 and size exclusion chromatography on Sephadex G-100 matrix. Molecular weight (16.6 kDa) was estimated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). The eﬀects of temperature, pH, metal chelation- and larvicidal activity of ASL were also investigated. The ASL indiﬀerently agglutinated the erythrocytes of the human ABO blood system and was stable at acidic pH and below 50 °C whereas 66% of its activity was lost at 60 °C with complete inactivation at 70 °C. ASL is a metalloprotein requiring barium ion as chelation of metals by 50 mM EDTA rendered the lectin inactive, while the addition of BaCl2, among other metal salts, restored the activity. ASL showed larvicidal activity against C. quinquefasciatus larvae after 24 h with a mortality of 5 and 95% at 5 and 25 mg/mL respectively, and LC50 of 13.80 mg/mL. This study concluded that purified A. semotus lectin showed impressive larvicidal activity, which could be exploited in its development as an insecticidal agent.","PeriodicalId":34918,"journal":{"name":"Acta Biologica Szegediensis","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66816315","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

The eﬀect of nickel phytotoxicity on photosystem II activity and antioxidant enzymes in barley 镍对大麦光系统II活性及抗氧化酶的影响

Acta Biologica Szegediensis Pub Date : 2021-01-01 DOI: 10.14232/abs.2021.1.1-9

Ali Doǧru, H. Altundağ, M. S. Dündar

{"title":"The eﬀect of nickel phytotoxicity on photosystem II activity and antioxidant enzymes in barley","authors":"Ali Doǧru, H. Altundağ, M. S. Dündar","doi":"10.14232/abs.2021.1.1-9","DOIUrl":"https://doi.org/10.14232/abs.2021.1.1-9","url":null,"abstract":"In this study, the eﬀect of mild (100 µM), moderate (300 µM) and severe (500 µM) nickel (NiSO4.7H2O) toxicity on the photosynthetic activity, photosynthetic pigment content and some antioxidant enzymes in the leaves of a barley cultivars (Hordeum vulgare L. cv. Tarm-92) was investigated. Moderate and severe nickel toxicity decreased root length while shoot length was not aﬀected by nickel stress, probably due to over accumulation of nickel in roots. Similarly, biomass accumulation was declined by moderate and severe nickel toxicity as reﬂected by the lowered fresh and dry weight. Chlorophyll a, chlorophyll b and consequently total chlorophyll content decreased by all nickel applications, presumably because the reduced level of carotenoids. Chlorophyll a ﬂuorescence measurements showed that nickel toxicity blocked electron movement in some speciﬁc points of the photosynthetic electron transport system. The constant Fo value indicated that PSII reaction centers was not damaged in the leaves of barley under nickel toxicity while the reduced Fm value showed that acceptor side of PSII was more sensitive to nickel toxicity as compared to donor side. Changes in JIP test parameters in the leaves of barley showed that primary photochemical reactions are reduced, and thermal dissipation of excess energy is increased. SOD and CAT activity is elevated in the leaves of barley under moderate and severe nickel toxicity which demonstrate an eﬃcient superoxide dismutation. Severe nickel toxicity, however, did not aﬀect SOD and CAT activity. The ascorbate-glutathione cycle was activated in the leaves of barley plants under nickel toxicity, probably indicating an eﬃcient H2O2 detoxiﬁcation. However, considerable H2O2 and MDA accumulation was observed in the leaves of barley under nickel stress. As a result, it may be concluded that the barley genotype Tarm-92 is moderately tolerant to nickel toxicity.","PeriodicalId":34918,"journal":{"name":"Acta Biologica Szegediensis","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"66816245","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1