Acta Biologica Szegediensis最新文献

The pangenome structure of human pathogen Mycobacterium kansasii 人类致病菌堪萨斯分枝杆菌的泛基因组结构

Acta Biologica Szegediensis Pub Date : 2023-05-29 DOI: 10.14232/abs.2022.2.192-201

S. Sur

{"title":"The pangenome structure of human pathogen Mycobacterium kansasii","authors":"S. Sur","doi":"10.14232/abs.2022.2.192-201","DOIUrl":"https://doi.org/10.14232/abs.2022.2.192-201","url":null,"abstract":"The non-tuberculous Mycobacterium kansasii, is the causative agent of destructive pulmonary and extrapulmonary infections in immunocompromised persons. Incessant use of multiple antibiotics and lack of effective vaccines did little to combat M. kansasii mediated infections. Here, a bioinformatic analysis has been carried out using PanExplorer, to analyze the pangenome aimed at functional characterization of the bacterium, understanding it’s pathogenic lifestyle and recognize the factors shaping evolution and variations amongst strains. M. kansasii had a large core genome (60.2%), a small (11.9%) dispensable genome and 27.9% strain-specific genes. The core genome of M. kansasii had a high concentration of COGs (Cluster of orthologous genes) linked to energy production and conversion, amino acid transport and metabolism, nucleotide transport and metabolism, coenzyme transport and metabolism, and secondary me-tabolite biosynthesis, transport and metabolism. Interestingly, numerous genes within the core and dispensable genome were associated with pathogenesis and virulence. Noteworthy among them were type VII secretion, ESX, PP and PPE family proteins. Although, M. kansasii genomes revealed overall relatedness and conservation, genomic rearrangements caused variability within the strains. The information from this analysis could assist future microbial genomics research on M. kansasii, and further studies, e.g., concerning distinctive gene clusters, and evolution.","PeriodicalId":34918,"journal":{"name":"Acta Biologica Szegediensis","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45764955","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Drug repurposing in the context of common bacterial pathogens 在常见细菌病原体的背景下药物再利用

Acta Biologica Szegediensis Pub Date : 2023-05-27 DOI: 10.14232/abs.2022.2.140-149

M. Donadu, S. Zanetti, B. Battah, H. Hetta, Danica Matusovits, K. Kárpáti, Virág Finta, Berta Csontos, Anna Kuklis, Fruzsina Szikora, Adrienn Csegény, Lea Szalma, Eszter Major, I. Kushkevych, M. Gajdács

{"title":"Drug repurposing in the context of common bacterial pathogens","authors":"M. Donadu, S. Zanetti, B. Battah, H. Hetta, Danica Matusovits, K. Kárpáti, Virág Finta, Berta Csontos, Anna Kuklis, Fruzsina Szikora, Adrienn Csegény, Lea Szalma, Eszter Major, I. Kushkevych, M. Gajdács","doi":"10.14232/abs.2022.2.140-149","DOIUrl":"https://doi.org/10.14232/abs.2022.2.140-149","url":null,"abstract":"The clinical problem of multidrug resistance (MDR) in bacteria is due to the lack of novel antibiotics in development and the dwindling pipeline of drugs receiving market authorization. Repurposing of non-antibiotic pharmacological agents may be an attractive pathway to provide new antimicrobial drugs. The aim of the present study was to ascertain the antibacterial and adjuvant properties of a wide range of pharmaceuticals against antibiotic-susceptible and drug-resistant bacteria. Sixty-five (n = 65) pharmacological agents were included in our experiments. For Gram-positive bacteria, Staphylococcus aureus ATCC 43300 (methicillin-resistant), S. epidermidis ATCC 12228, Streptococcus pyogenes ATCC 12384 and Enterococcus faecalis ATCC 29212 were used, while for Gram-negative bacteria, Enterobacter cloacae ATCC 13047 (extended-spectrum β-lactamase-positive), Klebsiella pneumoniae ATCC 49619, Serratia marcescens ATCC 29632 and Pseudomonas aeruginosa ATCC 27853 were included as representative strains. The minimum inhibitory concentrations (MICs) of the tested compounds were determined using the standard broth microdilution method, while a MIC reduction assay was included to ascertain the effect of the tested compounds on the MICs of standard antibiotics (ceftriaxone, ciprofloxacin and gentamicin). Seventeen and twelve drug molecules tested showed measurable antibacterial activities (MIC: 32-512 µg/mL) against Gram-positive and Gram-negative bacteria, respectively. Several compounds decreased the MICs of ciprofloxacin and gentamicin. Although there are increasing number of studies in this field, there are still significant gaps in the evidence to the potential use of non-antibiotic drugs in antimicrobial drug repurposing.","PeriodicalId":34918,"journal":{"name":"Acta Biologica Szegediensis","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48264996","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigating crossed-symmetry pattern in children from the past 儿童交叉对称模式的研究

Acta Biologica Szegediensis Pub Date : 2023-05-27 DOI: 10.14232/abs.2022.2.126-131

Ágnes Fogl, G. Pálfi, I. Maák, J. Balázs

引用次数: 0

Investigation of the efficiency of some fungicides and disinfectants applied in Agaricus bisporus cultivation 几种杀菌剂和消毒剂在双孢蘑菇栽培中的应用效果研究

Acta Biologica Szegediensis Pub Date : 2023-05-27 DOI: 10.14232/abs.2022.2.100-105

András Misz, Henrietta Allaga, R. Büchner, A. Varga, N. Bakos-Barczi, Judit Bajzát, L. Kredics, C. Csutoras, C. Vágvölgyi

{"title":"Investigation of the efficiency of some fungicides and disinfectants applied in Agaricus bisporus cultivation","authors":"András Misz, Henrietta Allaga, R. Büchner, A. Varga, N. Bakos-Barczi, Judit Bajzát, L. Kredics, C. Csutoras, C. Vágvölgyi","doi":"10.14232/abs.2022.2.100-105","DOIUrl":"https://doi.org/10.14232/abs.2022.2.100-105","url":null,"abstract":"Pathogenic moulds (e.g., Trichoderma, Hypomyces, and Lecanicillium) cause huge problems in industrial scale production of Agaricus bisporus. It is important to choose the right control strategy, either chemical or biocontrol-based, to prevent substantial economic losses. The present study was carried out to reveal the in vitro efficacy of some fungicides and disinfectants against causative agents of wet bubble (Hypomyces perniciosus), dry bubble (Lecanicillium fungicola var. fungicola), cobweb disease (Hypomyces odoratus), and green mould (e.g., Trichoderma aggressivum f. aggressivum, T. aggressivum f. europaeum, T. harzianum), the four most devastating fungal infections in mushroom production. Prochloraz was efficient against Trichoderma and H. perniciosus isolates. Metrafenone did not cause complete inhibition for any of the isolates even at the highest concentration (5%) tested. In both cases Lecanicillium isolates displayed the lowest growth rate inhibition. Concerning the tested disinfectants, treatment with Sekusept Aktiv resulted in complete growth inhibition for all isolates in the concentration range of 1.25-5%. Terralin Protect, Disinflex and Formalin were also effective, except against the tested isolates of T. agressivum f. agressivum which proved to be the most resistant.","PeriodicalId":34918,"journal":{"name":"Acta Biologica Szegediensis","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48020016","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Heavy metals tolerance on seed germination and growth of serpentine plant species Alyssum murale L. 蛇纹草种子萌发和生长对重金属的耐受性。

Acta Biologica Szegediensis Pub Date : 2023-05-27 DOI: 10.14232/abs.2022.2.116-124

B. Gashi, Mirsade Osmani, Naim Berisha, Liridon Buqaj, Besnik Dakaj, Eliesa Qazimi, Erëza Çoçaj

{"title":"Heavy metals tolerance on seed germination and growth of serpentine plant species Alyssum murale L.","authors":"B. Gashi, Mirsade Osmani, Naim Berisha, Liridon Buqaj, Besnik Dakaj, Eliesa Qazimi, Erëza Çoçaj","doi":"10.14232/abs.2022.2.116-124","DOIUrl":"https://doi.org/10.14232/abs.2022.2.116-124","url":null,"abstract":"Alyssum murale L. is a nickel hyperaccumulator herbaceous perennial species in a natural serpentine soil and adapted to arid, infertile soils. The objective of this study was to investigate how the seed germination, root, and hypocotyl growth of this plant species respond to exposure to different concentrations of heavy metals such as lead (Pb), cadmium (Cd), nickel (Ni), chromium (Cr) and manganese (Mn). The highest germination, even higher than in the control group, was observed at lower concentrations of Ni, Cd, Pb and Mn, while almost all higher concentrations of the metals decreased germination. Germination time was significantly slower (from about 6 to 10 days) for seeds treated with higher metal concentrations, especially Mn, Cd and Ni, and faster (from about 3 to 5 days) for seeds treated with lower concentrations. The strongest inhibitory effect on root and hypocotyl length occurred in treatments with different concentrations of Pb, Cd, and Mn, and seeds treated with lower concentrations of Ni showed a positive effect on root and hypocotyl growth. Seeds treated with Ni showed relatively high tolerance to this metal, presenting the potential for practical use in various fields of phytoremediation technology.","PeriodicalId":34918,"journal":{"name":"Acta Biologica Szegediensis","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43687293","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of intraductal drug delivery of orexin receptor antagonists into lactating rat mammary gland on milk cholesterol metabolism by regulating Fas and Hmgcr genes 食欲素受体拮抗剂乳管内给药对Fas和Hmgcr基因调节乳胆固醇代谢的影响

Acta Biologica Szegediensis Pub Date : 2023-05-27 DOI: 10.14232/abs.2022.2.156-161

Shima Jafari Zandieh, H. Khazali

{"title":"Effect of intraductal drug delivery of orexin receptor antagonists into lactating rat mammary gland on milk cholesterol metabolism by regulating Fas and Hmgcr genes","authors":"Shima Jafari Zandieh, H. Khazali","doi":"10.14232/abs.2022.2.156-161","DOIUrl":"https://doi.org/10.14232/abs.2022.2.156-161","url":null,"abstract":"In recent years, many studies have demonstrated that the system of orexin plays a pivotal role in regulating lipogenesis enzymes. However, its effect on the mammary glands is not entirely known. This study answers the question of whether intra-ductal injection of orexin antagonists (OX1RA and OX2RA) into the mammary glands can result in the expression of fatty acid synthase (Fas) and HMG-CoA reductase (Hmgcr) genes and the secretion of cholesterol in lactating female rats or not. To this end, 42 Lactating rats were randomly divided into experimental groups including a control group and groups receiving OX1RA and OX2RA intraductal (with doses of 5, 10, and 20 µg/kg, i.duc). Milk samples were collected for cholesterol testing. Using specific primers for each gene, the target genes were measured via real-time PCR. Data differences were considered significant with P <0.05. PCR exhibited that the injection of orexin antagonists significantly reduced Fas and Hmgcr gene expression. Moreover, the injection of antagonists significantly reduced milk cholesterol. Intra-mammary injection of orexin antagonists reduces milk cholesterol levels by affecting the expression of Fas and Hmgcr genes.","PeriodicalId":34918,"journal":{"name":"Acta Biologica Szegediensis","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46530269","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative mathematical analysis of economically important ampelographic indicators in white wine vine varieties (Vitis vinifera L.) 白葡萄酒葡萄品种（Vitis vinifera L.）重要经济性状的比较数学分析

Acta Biologica Szegediensis Pub Date : 2023-05-27 DOI: 10.14232/abs.2022.2.106-115

V. Roychev, N. Keranova

{"title":"Comparative mathematical analysis of economically important ampelographic indicators in white wine vine varieties (Vitis vinifera L.)","authors":"V. Roychev, N. Keranova","doi":"10.14232/abs.2022.2.106-115","DOIUrl":"https://doi.org/10.14232/abs.2022.2.106-115","url":null,"abstract":"A comparative mathematical analysis of economically important ampelographic indicators was performed in 32 local, introduced and newly bred white wine vine varieties. It was found that depending on the similarity and remoteness of the values of the studied indicators, the varieties are grouped into three generalized clusters. In the first, the varieties are characterized by a longer budding period and relatively lower productivity. In the second, they have similarities in the length of the budding period, long period of budding - technological maturity, moderate productivity, and close content of volatile acids in the wine. In the third, the newly bred two varieties Gergana and Misket Markovski, which are the most fertile and productive and with the smallest number of seeds in the berries. There is a high variability of the individual white wine varieties according to the studied economically valuable indicators. The indicators of bunch length and average weight per 100 berries have a stronger direct effect on the formation of the yield in individual varieties, and the average bunch weight, bunch width, percentage of mesocarp in berries, berry length and width, as well as the theoretical yield significantly affect indirectly the productivity of vines.","PeriodicalId":34918,"journal":{"name":"Acta Biologica Szegediensis","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43658169","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A longitudinal study on the prevalence of iron deficiency anemia in the multiethnic communities of Jhargram, West Bengal 西孟加拉邦贾格拉姆多民族社区缺铁性贫血患病率的纵向研究

Acta Biologica Szegediensis Pub Date : 2023-05-27 DOI: 10.14232/abs.2022.2.180-191

Moumi Mandal, Niladri Prasad Mishra, P. Chatterjee, Sanchari Bhattacharyya, K. C. Mondal, Sagar Acharya

{"title":"A longitudinal study on the prevalence of iron deficiency anemia in the multiethnic communities of Jhargram, West Bengal","authors":"Moumi Mandal, Niladri Prasad Mishra, P. Chatterjee, Sanchari Bhattacharyya, K. C. Mondal, Sagar Acharya","doi":"10.14232/abs.2022.2.180-191","DOIUrl":"https://doi.org/10.14232/abs.2022.2.180-191","url":null,"abstract":"Anemia is a significant public health concern in India, with an esti-mated prevalence of 38% to 82% in non-pregnant females and 18% to 59% in males. Destitution, poor nutrition, illiteracy, unhealthy practices, obliviousness, and inadequate health service is the significant reasons for the higher rate of IDA in the tribals of India. The objective of the present study was to estimate the disease burden in Binpur-I and II, Jhargram blocks in West Bengal, India. The research was planned as a multiethnic community-based investigation that includes age and sex-specific gradation of anemia with iron status (SF, SI, TIBC). 910 participants (female: 529 and male: 381) were enrolled in the study. Overall, 67.47% of individuals were diagnosed with anemia (female: 42.74% and male: 24.73% ). According to severity, 29.12%, 27.14%, and 11.2% of individuals were moderate, mild, and severely anemic. Results also showed that IDA is prevalent among all age groups irrespective of sex, with the highest rates in the 40.1 - 60 age group (female). The higher prevalence of the geriatric population could be due to chronic disease, iron deficiency, and genetic polymorphisms, which require future investigations.","PeriodicalId":34918,"journal":{"name":"Acta Biologica Szegediensis","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43232286","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hepatoprotective effect of the methanol extract of Luffa cylindrica fruit on carbon-tetrachloride induced chronic liver injury 丝瓜果甲醇提取物对四氯化碳所致慢性肝损伤的保护作用

Acta Biologica Szegediensis Pub Date : 2023-05-27 DOI: 10.14232/abs.2022.2.150-155

I. Oyeyemi, Oluwabukola O. Akanni, O. Adaramoye, A. Bakare

引用次数: 0

Effect of water deprivation for three consecutive days on the proportions of androgen receptor-immunolabeled supraoptic nucleus magnoneurons in Wistar rats 连续三天脱水对Wistar大鼠雄激素受体免疫标记视上核磁神经元比例的影响

Acta Biologica Szegediensis Pub Date : 2023-05-27 DOI: 10.14232/abs.2022.2.162-168

Samir Chetoui, H. Touati, Soumia Remana, Nadir Benhaferi, Nadia Hanniche, Amina Talmatamar, Souhila Benarab, Abdelkader Bougrid

{"title":"Effect of water deprivation for three consecutive days on the proportions of androgen receptor-immunolabeled supraoptic nucleus magnoneurons in Wistar rats","authors":"Samir Chetoui, H. Touati, Soumia Remana, Nadir Benhaferi, Nadia Hanniche, Amina Talmatamar, Souhila Benarab, Abdelkader Bougrid","doi":"10.14232/abs.2022.2.162-168","DOIUrl":"https://doi.org/10.14232/abs.2022.2.162-168","url":null,"abstract":"The androgen receptor is an androgen-dependent transcription factor that belongs to the nuclear receptor superfamily. When the androgen receptor is not bound to its ligand, it is mainly localized in the cytoplasm, bound to chaperone proteins, which stabilizes its inactive conformation and confers a high affinity for the ligand. The conformational change of the androgen receptor begins when the androgen molecule binds to the receptor, which subsequently homodimerizes and it is actively translocated to the nucleus. In the nucleus, the receptor homodimers bind to androgen target genes, resulting in responses such as growth and survival. In this study, we used the immunohistochemistry technique to look for the proportions of androgen receptor-immunolabeled magnoneurons categories according to the sub-cellular localization of the receptor, in the supraoptic nucleus of control (tap water + ad-libitum food) and dehydrated male rats by tap water deprivation for three consecutive days. Our results show that the proportions of androgen receptor-immunolabeled magnoneurons would depend on the hydration status of the rats (Chi-square test of independence, P < 0.001), but this dependence relationship is weak (Cramer’s v value is equal to 0.30). Indeed, based on the results of our study, we hypothesized that dehydration by water deprivation for three consecutive days in adult male rats acts mainly on both of nuclear and cytoplasmic magnoneurons categories and has very little influence on the nucleocytoplasmic magnoneurons category. The effect could be due to activation of the entry of cytoplasmic non-androgen bound androgen receptors into the nucleus, activation of degradation of nuclear non-androgen bound androgen receptors, and inhibition of the binding of androgen molecules to androgen receptors.","PeriodicalId":34918,"journal":{"name":"Acta Biologica Szegediensis","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41639089","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0