Journal of Insect Biotechnology and Sericology最新文献

{"title":"Morphological and histomorphological structures of testes and ovaries in early developmental stages of the silkworm, <i>Bombyx mori</i>.","authors":"Hiroki Sakai,&nbsp;Yohei Kirino,&nbsp;Susumu Katsuma,&nbsp;Fugaku Aoki,&nbsp;Masataka G Suzuki","doi":"10.11416/jibs.85.1_015","DOIUrl":"https://doi.org/10.11416/jibs.85.1_015","url":null,"abstract":"<p><p>The gonad develops as a testis in male or an ovary in female. In the silkworm, <i>B. mori</i>, little is known about testis and ovary in the embryonic stages and early larval stages. In this study, we performed morphological and histomorphological observations of ovaries and testes from the late embryonic stage to the 1st instar larval stage. Results obtained with lack of accurate information on sex of examined individuals may be misleading, thus we performed phenotypic observations of gonads by utilizing sex-limited strain that enables us to easily discriminate female embryos from male ones based on those egg colors. In testis, four testicular follicles were clearly observed in the testis at the first instar larval stage, and boundary layers were formed between the testicular follicles. At the late embryonic stage, the testis consisted of four testicular follicles, while the boundary layers were still obscure. In ovary, four ovarioles were easily recognizable in the ovary at the first instar larval stage, and boundary layers were formed between the ovarioles. However, in the late embryonic stage, it was quite difficult to identify four ovarioles. Morphological characteristics were almost similar between testis and ovary in early developmental stages. Our present study demonstrates that the most reliable difference between testis and ovary in early developmental stages is the attaching point of the duct. Formation and development of the duct may be sensitive to the sex-determining signal and display sexual dimorphism in early embryonic stages.</p>","PeriodicalId":34896,"journal":{"name":"Journal of Insect Biotechnology and Sericology","volume":"85 1","pages":"15-20"},"PeriodicalIF":0.0,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.11416/jibs.85.1_015","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35541933","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A simple method for homozygotic selection of Nd genes using artificial diet rearing in the silkworm, Bombyx mori","authors":"Y. Takemura, A. Ohnuma, Yuji Mochida, M. Mastumoto","doi":"10.11416/JIBS.85.1_001","DOIUrl":"https://doi.org/10.11416/JIBS.85.1_001","url":null,"abstract":"","PeriodicalId":34896,"journal":{"name":"Journal of Insect Biotechnology and Sericology","volume":"85 1","pages":"1-6"},"PeriodicalIF":0.0,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"63988191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"rRNA degradation in Bombyx mori and Bombyx mandarina cells infected with heterologous nucleopolyhedroviruses","authors":"Rina Hamajima, C. Yasunaga–Aoki, M. Iwanaga, S. Imanishi, J. Kobayashi, Kuni Sasaki, T. Kusakabe, J. M. Lee, H. Mon, Michihiro Kobayashi, M. Ikeda","doi":"10.11416/JIBS.85.3_073","DOIUrl":"https://doi.org/10.11416/JIBS.85.3_073","url":null,"abstract":"","PeriodicalId":34896,"journal":{"name":"Journal of Insect Biotechnology and Sericology","volume":"85 1","pages":"73-77"},"PeriodicalIF":0.0,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"63988624","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterization of the roles of DNA polymerases, clamp, and clamp loaders during S-phase progression and cell cycle regulation in the silkworm, Bombyx mori","authors":"M. Hino, Daisuke Morokuma, H. Mon, J. M. Lee, T. Kusakabe","doi":"10.11416/JIBS.85.2_021","DOIUrl":"https://doi.org/10.11416/JIBS.85.2_021","url":null,"abstract":"","PeriodicalId":34896,"journal":{"name":"Journal of Insect Biotechnology and Sericology","volume":"85 1","pages":"21-29"},"PeriodicalIF":0.0,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"63987921","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Absence of hybrids between the domesticated silkmoth, Bombyx mori, and the wild mulberry silkmoth, B. mandarina, in natural populations around sericulture farms","authors":"N. Kômoto, Nobuo Kuwabara, K. Yukuhiro","doi":"10.11416/JIBS.85.3_067","DOIUrl":"https://doi.org/10.11416/JIBS.85.3_067","url":null,"abstract":"","PeriodicalId":34896,"journal":{"name":"Journal of Insect Biotechnology and Sericology","volume":"85 1","pages":"67-71"},"PeriodicalIF":0.0,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"63988799","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Inhibitory effects on silk fibroin production by the expression of phenylalanyl-tRNA synthetase mutants in posterior silk glands of Bombyx mori","authors":"Hidetoshi Teramoto, K. Kojima","doi":"10.11416/JIBS.85.2_031","DOIUrl":"https://doi.org/10.11416/JIBS.85.2_031","url":null,"abstract":"","PeriodicalId":34896,"journal":{"name":"Journal of Insect Biotechnology and Sericology","volume":"85 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"63988451","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Structural analysis of spontaneous Z-W translocations in the silkworm, Bombyx mori","authors":"T. Fujii, A. Ohnuma, Y. Banno, H. Abe","doi":"10.11416/JIBS.85.3_079","DOIUrl":"https://doi.org/10.11416/JIBS.85.3_079","url":null,"abstract":"In the silkworm, Bombyx mori, femaleness is determined by the presence of the W chromosome irrespective of the number of Z chromosomes (Hashimoto, 1933). During meiosis in females, all chromosomes, including sex chromosomes, form bivalents (Traut et al., 1999). However, the W chromosome is recombinationally isolated from the Z chromosome in B. mori, because meiosis is achiasmatic, and no recombination occurs in females (Sahara et al., 2012). In sericulture, male silkworms are more viable and productive than female silkworms (Strunnikov, 1995). The sex-linked balanced lethal (SLBL) strains make it possible to rear only male silkworms, thus improving the production and quality of silk. The construction of an SLBL strain requires two steps: (i) induction of translocation events between the W and Z chromosomes and (ii) induction of Z-linked recessive lethal mutations, which are compensated by the dominant allele translocated onto the W chromosome (Strunnikov, 1995). In Japan, one such SLBL strain “PLATINA BOY,” has been introduced for commercial use in the production of luxury fabrics (Ohnuma, 2007, 2010). Generally, translocations and mutations are induced by irradiation or exposure to a chemical mutagen. However, Ohnuma has developed a strategy for obtaining T(W;Z) chromosomes and Z-linked recessive lethal mutations arising from frequent spontaneous translocation events between the T(W;Z)+ chromosome and T(Z;2)Y females (Fig. 1a, b) (Ohnuma, 2000; Ohnuma and Takemura, 2006; Ohnuma et al., 2009). He effectively used the sch gene and sex-linked recessive lethal mutations, and screened translocations and mutations soon after hatching (Fig. 1a, b). In all, 25 types of aberrant chromosomes were identified (Ohnuma, 2000; Ohnuma and Takemura, 2006; Ohnuma et al., 2009; Ohnuma, 2010). Unfortunately, most of them, except for T(Z;W)l4, T(Z;W)l7, T(W;Z;2)Y-3, and T(W;Z;2)Y-4, were discarded without any analysis. However, the remaining four aberrant sex chromosomes are novel bioresources and it is of great interest to determine whether the breakpoints of the spontaneous translocation events reside within the W-chromosomal region or Z-chromosomal region of the T(W;Z)+ chromosome. Genome data is powerful tool to characterize chromosomal aberrations. In the Bombyx genome project, most areas of the Bombyx genome were assembled into large blocks of sequences (International Silkworm Genome Consortium, 2008). However, the W chromosome sequences were not included in the genome data, because the male genome was subjected to a whole-genome shotgun analysis to avoid the repetitive nature of the W chromosome (Abe et al., 2005a; Sahara et al., 2003). On the other hand, twelve W-chromosome specific sequences have been identified by screening arbitrary 10-mer primers, which yielded female-specific amplification products (Abe et al., 2005b). These sequences have been used to characterize the structural mutants of the W chromosome (Abe et al., 2005b; 2008; Fujii et al., 2006; ","PeriodicalId":34896,"journal":{"name":"Journal of Insect Biotechnology and Sericology","volume":"78 1","pages":"79-85"},"PeriodicalIF":0.0,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"63988723","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immune response in the hemocyte after phagocytosis influences the infection with Beauveria brongniartii to the silkworm, Bombyx mori","authors":"S. Wada, W. Mitsuhashi, K. Miyamoto, Y. Kunimi","doi":"10.11416/JIBS.85.3_055","DOIUrl":"https://doi.org/10.11416/JIBS.85.3_055","url":null,"abstract":"","PeriodicalId":34896,"journal":{"name":"Journal of Insect Biotechnology and Sericology","volume":"85 1","pages":"55-65"},"PeriodicalIF":0.0,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.11416/JIBS.85.3_055","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"63988742","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Differential N-Glycan Modifications of Human Alpha 1-Acid Glycoprotein (α1AGP) Produced in Different Silkworm Strains using the Baculovirus Expression System","authors":"Daisuke Morokuma, H. Mon, Y. Banno, T. Kusakabe, J. M. Lee","doi":"10.11416/JIBS.84.2_049","DOIUrl":"https://doi.org/10.11416/JIBS.84.2_049","url":null,"abstract":"","PeriodicalId":34896,"journal":{"name":"Journal of Insect Biotechnology and Sericology","volume":"84 1","pages":"49-53"},"PeriodicalIF":0.0,"publicationDate":"2015-11-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"63988278","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Aquaporins are expressed in the columnar cells of the midgut epithelium of the silkworm, Bombyx mori","authors":"Mariya Maruyama, M. Azuma","doi":"10.11416/JIBS.84.3_055","DOIUrl":"https://doi.org/10.11416/JIBS.84.3_055","url":null,"abstract":"","PeriodicalId":34896,"journal":{"name":"Journal of Insect Biotechnology and Sericology","volume":"84 1","pages":"55-61"},"PeriodicalIF":0.0,"publicationDate":"2015-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"63987840","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}