Journal of Insect Biotechnology and Sericology最新文献_第4页

Behavior of the larvae of wild mulberry silkworm Bombyx mandarina, domesticated silkworm B. mori and their hybrid 野桑蚕、家蚕及其杂交种幼虫的行为

Journal of Insect Biotechnology and Sericology Pub Date : 2017-01-01 DOI: 10.11416/JIBS.86.1_017

N. Kômoto

引用次数: 6

Identifying a sigma class glutathione S-transferase 2 from the silkworm Bombyx mori 家蚕谷胱甘肽s -转移酶2的鉴定

Journal of Insect Biotechnology and Sericology Pub Date : 2017-01-01 DOI: 10.11416/JIBS.86.1_001

A. Hirowatari, S. Nagaoka, N. Yamada, K. Yamamoto

引用次数: 1

Relative humidity and maintenance of p50 silkworms reared on artificial diet 人工饲料饲养p50家蚕的相对湿度及维持

Journal of Insect Biotechnology and Sericology Pub Date : 2017-01-01 DOI: 10.11416/JIBS.86.2_043

Kodai Yuri, K. Sahara

{"title":"Relative humidity and maintenance of p50 silkworms reared on artificial diet","authors":"Kodai Yuri, K. Sahara","doi":"10.11416/JIBS.86.2_043","DOIUrl":"https://doi.org/10.11416/JIBS.86.2_043","url":null,"abstract":"Silk spinners (e.g. spiders, moths, mites, mayflies) and cocoon makers (e.g. spiders, moths, bees, flies) have repeatedly evolved in arthropods, and human beings have selected some of these moths for use in silk production. Environments sometimes harmfully affect cocoon quality as well as the spinners that inhabit them. For instance, the parasitoid bee, Cotesia glomerata, forms cocoon clusters to protect themselves from hyperparasitism (Tanaka and Ohsaki, 2006). However, high humidity causes individual cocoons to be thinner and the clusters to be loose (Tagawa and Satoh, 2008). The harmful effects of high humidity on the quality of cocoons and silk threads are well known in sericulture (Yamamoto, 1975; Kataoka, 1977). Recent experiments have shown that very high humidity negatively affects successful mounting and results in an increase of naked pupae (Watanabe et al., 2014). These are different from genetic naked-pupa (Nd) (Nakao, 1950) that cannot produce fibroin, which leads either to cocoons being constructed exclusively of sericin or to naked pupae. The frequency of adult eclosion has been found to be 88.7% in the naked pupae of Nd mutants (Nakao, 1950). Another naked pupae can be experimentally induced by blocking the spinneret opening, causing them to be naked and thus not become adults (Akao, 1942). These results suggest that physically induced naked pupae are defective and unable to become moths. Two silkworm genome data were deposited into public databases, one originating in Japan (Kaikobase: http://sgp. dna.affrc.go.jp/KAIKObase/) and the other in China (SilkDB: http://silkworm.genomics.org.cn/). These opensource databases have been widely used by entomologists, especially lepidopteran molecular scientists (The International Silkworm Genome Consortium, 2008; Daimon et al., 2014; Xia et al., 2014). The strains, p50T (Daizo) and Dazao used for the respective genome projects, should be very close relatives. A p50T is a single-paired descendant of individuals of strain p50 (a derivative from Daizo kept in the national bioresource project (NBRP) Silkworm) (see http://shigen.nig.ac.jp/silkwormbase/top.jsp). Since the p50 individuals are available through the NBRP Silkworm project and are possible to rear not only with mulberry leaves but also with artificial diets (e.g. Silk Mate, NOSAN Corporation), these silkworms are one of the most important reference strains for lepidopteran studies (Fujiwara and Nishikawa, 2016). Artificial diet rearing has advantages in keeping the silkworms in an incubator throughout the year. In sericulture adequate temperatures for rearing silkworms with mulberry leaves are considered at 27-29°C for 1st instar larvae. Then, breeders may decrease temperature at 1°C after each molting up to 22-24°C in the last instar larvae (The Japanese Society of Sericultural Science, 1979). Relative humidity (RH) is recommended at 85-90% in the beginning and to decrease at approximately 5% in every ecdysis until last instar stage ","PeriodicalId":34896,"journal":{"name":"Journal of Insect Biotechnology and Sericology","volume":"86 1","pages":"43-47"},"PeriodicalIF":0.0,"publicationDate":"2017-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"63988998","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification and localization of a novel ecdysone oxidase in the silkworm, bombyx mori 家蚕一种新型蜕皮激素氧化酶的鉴定与定位

Journal of Insect Biotechnology and Sericology Pub Date : 2017-01-01 DOI: 10.11416/JIBS.86.2_049

K. Yamamoto, S. Nagaoka

引用次数: 1

Design of a specific genetic marker for the classification of Bacillus thuringiensis 苏云金芽孢杆菌分类特异遗传标记的设计

Journal of Insect Biotechnology and Sericology Pub Date : 2017-01-01 DOI: 10.11416/JIBS.86.1_021

Moe Takahashi, Y. Hatakeyama, Yuji Imura, Haruka Nakamura, Sayuka Chiba, Naoki Kurimoto, Syouhei Miyake, Yuuichi Yamamoto, Kazuyo Enomoto, H. Iwano

引用次数: 1

A reconsideration of the taxonomic position of two bacterial strains isolated from flacherie-diseased silkworms in 1965. 1965年从家蚕中分离的两株细菌的分类地位的重新考虑。

Journal of Insect Biotechnology and Sericology Pub Date : 2017-01-01 DOI: 10.11416/JIBS.86.2_035

K. Iiyama, Mai Morishita, J. M. Lee, H. Mon, T. Kusakabe, K. Tashiro, T. Akasaka, C. Yasunaga–Aoki, K. Miyamoto

引用次数: 3

Genetic studies on two egg mutants, “small size egg” and “lethal non- diapausing egg” in the silkworm, Bombyx mori 家蚕“小卵”和“致死性非滞育卵”两个卵突变体的遗传研究

Journal of Insect Biotechnology and Sericology Pub Date : 2017-01-01 DOI: 10.11416/JIBS.86.3_123

Y. Banno, T. Fujii, Hisayoshi Fukumori, Kimiko Yamamoto, J. Kobayashi

引用次数: 0

Virulence of lipopolysaccharide-deficient mutants of Serratia liquefaciens toward the silkworm, Bombyx mori 液化沙雷菌脂多糖缺陷突变体对家蚕的毒力研究

Journal of Insect Biotechnology and Sericology Pub Date : 2016-04-28 DOI: 10.11416/JIBS.85.1_007

E. Taira, H. Mon, J. M. Lee, T. Kusakabe, C. Yasunaga–Aoki, K. Iiyama

引用次数: 1

Generation of an infectious cDNA clone of Okushiri virus and its derivative capable of expressing an exogenous gene 能表达外源基因的Okushiri病毒感染性cDNA克隆及其衍生物的生成

Journal of Insect Biotechnology and Sericology Pub Date : 2016-01-01 DOI: 10.11416/JIBS.85.2_039

Yudistira Wahyu Kurnia, Ryosuke Fujita, Masanao Sato, H. Isawa, S. Asano, N. D. Binh, H. Bando

引用次数: 1

In vitro culture and low temperature incubation tolerance of staged embryos of the silkworm, Bombyx mori 家蚕分期胚的离体培养及低温培养耐受性

Journal of Insect Biotechnology and Sericology Pub Date : 2016-01-01 DOI: 10.11416/JIBS.85.2_049

Hisayoshi Fukumori, T. Fujii, Y. Banno

{"title":"In vitro culture and low temperature incubation tolerance of staged embryos of the silkworm, Bombyx mori","authors":"Hisayoshi Fukumori, T. Fujii, Y. Banno","doi":"10.11416/JIBS.85.2_049","DOIUrl":"https://doi.org/10.11416/JIBS.85.2_049","url":null,"abstract":"Development of cryopreservation for the silkworm, Bombyx mori, has been anticipated for a long time, and now two methods are available. One uses frozen sperm (Takemura and Kanda, 1999; Takemura et al., 2000) and the other uses frozen ovaries (Mochida et al., 2003, Banno et al., 2013). These methods are already in practice for most silkworm strains, however, a small number of strains are not available due to their low tolerance to low temperature. It is important to develop a greater variety of methods for safe preservation of the large collections of silkworm bio-resources. Recently, larval hatching from cryopreserved embryos kept in liquid nitrogen was reported in Drosophila melanogaster (Mazur et al., 1992) and in Pectinophora gossypiella (Rajamohan et al., 2013). These reports present a potential alternative to those previously developed for the silkworm. To perform in vitro culture of B. mori embryos, penetration of cryoprotectant into the embryo is necessary. But silkworm embryos are enveloped with chorions (egg shells), which prevents the penetration of the cryoprotectant. To improve the permeability of embryos, first, removal of chorions is essential, and these dechorionated eggs should be cultured in vitro. The possibility of using silkworm embryos for long-term preservation was studied twenty years ago by Imanishi et al. (1996). According to their report, embryos died when they were kept at −196°C. However, their experiments were limited by the selection of the stage and basic procedures, and more detailed experimentation is needed to determine the possibility of embryo use for cryopreservation. In this study, optimal developmental stages for in vitro culture of embryos were investigated. The tolerance of the embryos for low temperature incubation was tested as the next step, along with detailed observations of their morphological features and developmental stages. MATERIALS AND METHODS","PeriodicalId":34896,"journal":{"name":"Journal of Insect Biotechnology and Sericology","volume":"85 1","pages":"49-53"},"PeriodicalIF":0.0,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"63988691","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3