Ho Chi Minh City Open University Journal of Science Engineering and Technology最新文献_第2页

Studying the damping characteristics of the system of building and infrastructure considering the Soil-Structure Interaction 考虑土-结构相互作用的建筑及基础设施系统阻尼特性研究

Ho Chi Minh City Open University Journal of Science Engineering and Technology Pub Date : 2022-11-04 DOI: 10.46223/hcmcoujs.tech.en.12.2.2480.2022

Duong Hong Tham

{"title":"Studying the damping characteristics of the system of building and infrastructure considering the Soil-Structure Interaction","authors":"Duong Hong Tham","doi":"10.46223/hcmcoujs.tech.en.12.2.2480.2022","DOIUrl":"https://doi.org/10.46223/hcmcoujs.tech.en.12.2.2480.2022","url":null,"abstract":"This paper studies the damping characteristics of the soil foundation under a building concerning the Soil-Structure Interaction (SSI). By considering a multi-story building resting on a piled raft foundation as a typical SSI system, subjected to an earthquake (i.e., Chi Chi earthquake, Taiwan 1999), the time-dependent responses of specified locations in the foundation are recorded and analyzed, both in-time domain and frequency domain. Referring to prior knowledge about system identification and damping, this study suggests an overwhelming approach to determining the damping ratio for analyzing more thoroughly a system of SSI systems. Results are a general procedure to establish the damping matrix, namely the Caughey damping matrix which takes several frequencies into account. A different viewpoint that the connection of superstructure and infrastructure in series could help to estimate the damping, and contribute to a wide range of system identification. All these procedures are used to predict more properly the damping characteristics of a system of structures. Suggestions over the findings would be expected to contribute a more rigorous component to the analysis of high-rise buildings in the future.","PeriodicalId":34742,"journal":{"name":"Ho Chi Minh City Open University Journal of Science Engineering and Technology","volume":"64 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87804492","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Establishing protocol for detecting methylation of Ubiquitin carboxyl terminal hydrolase 1 gene’s promoter in nasopharyngeal carcinoma 建立鼻咽癌中泛素羧基末端水解酶1基因启动子甲基化检测方案

Ho Chi Minh City Open University Journal of Science Engineering and Technology Pub Date : 2022-11-04 DOI: 10.46223/hcmcoujs.tech.en.12.2.2542.2022

T. Hue, Huynh Thi Mong Tuyen, N. Truong, Dao Duy Tin, T. Khoa, L. Thuy, L. D. Thuan

{"title":"Establishing protocol for detecting methylation of Ubiquitin carboxyl terminal hydrolase 1 gene’s promoter in nasopharyngeal carcinoma","authors":"T. Hue, Huynh Thi Mong Tuyen, N. Truong, Dao Duy Tin, T. Khoa, L. Thuy, L. D. Thuan","doi":"10.46223/hcmcoujs.tech.en.12.2.2542.2022","DOIUrl":"https://doi.org/10.46223/hcmcoujs.tech.en.12.2.2542.2022","url":null,"abstract":"Background: The methylation of Ubiquitin Carboxyl Terminal Hydrolase 1 (UCHL1) gene’s promoter has been reported as the etiological factor of nasopharyngeal tumorigenesis. Purpose: This study is designed to establish a protocol for detecting methylation of UCHL1 gene’s promoter in nasopharyngeal carcinoma (NPC) in a Vietnamese population. Materials and methods: 10 samples of NPC biopsy tissues and 10 samples of non-cancerous swabs were collected from the local hospital. Chloroform/Phenol method and Nested-MSP assays were established to detect methylation of a target gene. Results: The isolated DNA reached purity and high concentration which were confirmed by the method of absorbance measurement at 260nm and 280nm. Additionally, the Nested-MSP products of methylation or unmethylation were analyzed and visualized in the agarose gel with the band of 169bps and 210bps, respectively. By sequencing, it was confirmed that the two sets of primer could distinguish the status of methylation and unmethylation of UCHL1 gene’s promoter. Conclusion: Our data suggested that the current protocol could successfully identify the status of methylation and/or unmethylation of UCHL1 gene’s promoter.","PeriodicalId":34742,"journal":{"name":"Ho Chi Minh City Open University Journal of Science Engineering and Technology","volume":"62 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91141293","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Isolation and characterization of full-length genes encoding the anti-human CD45 antibody from the hybridoma cell line 16E8-F2 16E8-F2杂交瘤细胞系抗人CD45抗体全长基因的分离与鉴定

Ho Chi Minh City Open University Journal of Science Engineering and Technology Pub Date : 2022-11-04 DOI: 10.46223/hcmcoujs.tech.en.12.2.2319.2022

N. Q. Huy, Ta Huong Giang, N. Quan

引用次数: 0

Mobile colistin resistance: Prevalence, mechanisms, and current detection methods 移动粘菌素耐药性:流行、机制和当前的检测方法

Ho Chi Minh City Open University Journal of Science Engineering and Technology Pub Date : 2022-11-04 DOI: 10.46223/hcmcoujs.tech.en.12.2.2193.2022

N. Q. Truong, L. Thuy, S. Egorova, Lidiia A. Kaftyreva, Hoang Quoc Cuong

{"title":"Mobile colistin resistance: Prevalence, mechanisms, and current detection methods","authors":"N. Q. Truong, L. Thuy, S. Egorova, Lidiia A. Kaftyreva, Hoang Quoc Cuong","doi":"10.46223/hcmcoujs.tech.en.12.2.2193.2022","DOIUrl":"https://doi.org/10.46223/hcmcoujs.tech.en.12.2.2193.2022","url":null,"abstract":"Colistin is considered the last-line antibiotic against multidrug-resistant Gram-negative bacterial infections. The global dissemination of Mobile colistin resistance mcr-harboring bacteria is threatening public health. Such isolates have been detected in various reservoirs including animals, food products, the environment, and humans. From these reservoirs, the isolates are approximate: 53% from animals; 39% from humans; 5% from the environment; and 3% from food. Bacterial distributions were: E.coli 91%; Salmonella 7%; and Klebsiella 2%. Among the 10 mcr variants, mcr-1 and mcr-9 are the most prevalent (up to 95% and 64.53%, respectively, in different studies) while other variants account for 5%. The coexistence of mcr and other antibiotic resistance genes in single isolates is a significant concern; mcr variants carried by different plasmid types increase antibiotic resistance and transfer of mcr genes to other bacteria. The hypothesis that the food chain is intimately involved in mcr gene transfer is supported by the presence of mcr-harboring isolates in animals and foods relative to humans. Cheaper, quicker, and more effective diagnostic tools for detecting colistin-resistant bacterial phenotypes and genotypes are essential and urgent. Currently, high quality tests include RPNP (99.3% specificity, 96.7% sensitivity) and MRPNP (95.4% specificity, ~100% sensitivity). LBJMR, CHROM agar, COL-APSE, and Super Polymyxin are now the best media to screen the bacteria, with near 100% selectivity. Multiplex PCR is a suitable method to quickly and accurately detect mcr genes in E. coli and Salmonella . Multi-drug resistant Gram-negative bacteria remain a global burden and need to have continuous and effective surveillance.","PeriodicalId":34742,"journal":{"name":"Ho Chi Minh City Open University Journal of Science Engineering and Technology","volume":"29 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85211184","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

History and applications of concrete steel composite structure 混凝土-钢组合结构的历史与应用

Ho Chi Minh City Open University Journal of Science Engineering and Technology Pub Date : 2022-11-04 DOI: 10.46223/hcmcoujs.tech.en.12.2.2467.2022

Chu Tuan Long, Pham Quoc Hoan, D. X. Quy

引用次数: 0

DNA isolation in processed chicken meat products (nugget) using modified DNeasy Mericon Food kit (Qiagen) 使用改良的DNeasy Mericon Food试剂盒(Qiagen)分离加工肉制品(鸡块)的DNA

Ho Chi Minh City Open University Journal of Science Engineering and Technology Pub Date : 2022-11-04 DOI: 10.46223/hcmcoujs.tech.en.12.2.2463.2022

Alfi Sophian, Utaminingsih Sri, Utami Dyah Sofia

{"title":"DNA isolation in processed chicken meat products (nugget) using modified DNeasy Mericon Food kit (Qiagen)","authors":"Alfi Sophian, Utaminingsih Sri, Utami Dyah Sofia","doi":"10.46223/hcmcoujs.tech.en.12.2.2463.2022","DOIUrl":"https://doi.org/10.46223/hcmcoujs.tech.en.12.2.2463.2022","url":null,"abstract":"DNA isolation is one of the important steps in conducting PCR-based or real-time PCR-based molecular analysis. This study aims to test the quality of DNA isolation extracted from samples of processed food products from chicken meat (chicken nuggets). The novelty of this research lies in the DNA isolation technique modified from the manual kit which is used to produce good-quality DNA isolation results. The extraction method used was the modified extraction method and the standard extraction method of the DNeasy Mericon Food kit. Analysis of DNA quality was measured using a nanophotometer, where the analysis of DNA quality was analyzed based on the parameters of concentration values and purity values. Based on research data, it is known that the modified DNA extraction method produces a concentration value of DNA isolation with an average concentration value of 1295.1 ng/µL. Meanwhile, the value of the purity of the isolated DNA was 2.10 on average. while the standard DNA extraction method resulted in an average DNA isolation concentration value of 111.53 ng/µL. While the value purity of the isolated DNA was at an average value of 1.926. From the research data analyzed, it was found that the modified DNA isolation method has the advantage that the concentration of isolated DNA obtained is higher than the standard method, while the purity value of the DNA isolated from the standard method has better purity when compared to the modified method.","PeriodicalId":34742,"journal":{"name":"Ho Chi Minh City Open University Journal of Science Engineering and Technology","volume":"72 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83438491","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

A preliminary study to establish the transfected CHO cell lines which highly express Trastuzumab - A biosimilar product of Herceptin 建立高表达赫赛汀生物仿制药曲妥珠单抗的转染CHO细胞系的初步研究

Ho Chi Minh City Open University Journal of Science Engineering and Technology Pub Date : 2022-11-04 DOI: 10.46223/hcmcoujs.tech.en.12.2.2172.2022

Nguyen Quang Huy, Huỳnh Thị Ánh Ly, T. Mai, N. T. Truc, H. T. Hieu, Nguyen Mai Khoi, D. C. Chung, Trinh Thanh Hung, D. Si

{"title":"A preliminary study to establish the transfected CHO cell lines which highly express Trastuzumab - A biosimilar product of Herceptin","authors":"Nguyen Quang Huy, Huỳnh Thị Ánh Ly, T. Mai, N. T. Truc, H. T. Hieu, Nguyen Mai Khoi, D. C. Chung, Trinh Thanh Hung, D. Si","doi":"10.46223/hcmcoujs.tech.en.12.2.2172.2022","DOIUrl":"https://doi.org/10.46223/hcmcoujs.tech.en.12.2.2172.2022","url":null,"abstract":"Human epidermal growth factor receptor 2 (HER2) has been identified as a molecular target for breast cancer therapy, such as Trastuzumab (Herceptin®). This has been shown to improve patient survival substantially. The current study is aiming to locally produce an anti-HER2 monoclonal antibody (named Trastuzumab) which has an equivalent biological properties in comparison with the original version, Herceptin®). In silico design and construction of recombinant vectors, as well as the establishment of transfected cell lines with high expression of Trastuzumab were performed. Based on the protein sequences obtained from the Drugbank, the DNA sequences encoding for the light chain (Tras-Lc) and heavy chain (Tras-Hc) of Trastuzumab were optimized and integrated into pNanogen-Hygro and pNanogen-Puro vectors, respectively. The Neon Transfection System was used to co-transfect the pNanogen-Tras-Lc-Hygro and pNanogen-Tras-Hc-Puro constructs into CHO cells. Different co-transfected single-cell-colonies selected on media supplemented with hygromycin and puromycin were used for ELISA and SDS-PAGE assays to identify the CHO cell lines which highly express Trastuzumab. Based on the present results, 30μg of both constructs were suitable for DNA co-transfection. After 07 days of culture, the highest amount of Trastuzumab (561 µg/ml) was obtained from the H06LD68 cell line.","PeriodicalId":34742,"journal":{"name":"Ho Chi Minh City Open University Journal of Science Engineering and Technology","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79751667","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antioxidant activity of isolated compounds from ethyl acetate extract of Bruguiera parviflora 小花布鲁氏菌乙酸乙酯提取物中分离化合物的抗氧化活性

Ho Chi Minh City Open University Journal of Science Engineering and Technology Pub Date : 2022-10-06 DOI: 10.46223/hcmcoujs.tech.en.12.2.2329.2022

Nguyen Thi Thuy, B. Tùng

引用次数: 0

Towards the development of RSU augmented campus: An augmented reality campus guide for Romblon State University 面向RSU增强校园的发展:隆布隆州立大学增强现实校园指南

Ho Chi Minh City Open University Journal of Science Engineering and Technology Pub Date : 2022-04-20 DOI: 10.46223/hcmcoujs.tech.en.12.1.2218.2022

Ricky Michael Oliva, Labicane Malapote Gianinna Elaine

{"title":"Towards the development of RSU augmented campus: An augmented reality campus guide for Romblon State University","authors":"Ricky Michael Oliva, Labicane Malapote Gianinna Elaine","doi":"10.46223/hcmcoujs.tech.en.12.1.2218.2022","DOIUrl":"https://doi.org/10.46223/hcmcoujs.tech.en.12.1.2218.2022","url":null,"abstract":"In this era of emerging technologies, the application of the new trends has been accepted and applied in the fields of medication, education, sports, and infrastructure. Along with current innovations comes the birth of Augmented Reality (AR), a new technology that imposes 3D images and visuals into the real world. This study aims to automate and augment the process of locating campus buildings and offices by providing information as well navigation by scanning the infrastructures' image trackers using the process of augmentation. The application will elevate the in-campus experience of the students and visitors by providing an entertaining and efficient way of navigating through augmentation. To assess the user acceptability of the application, survey questionnaires were distributed through Google forms. As for the respondents, 450 students and 50 faculty personnel were considered as the sample size. Results reveal that users believe that the application will be able to provide helpful information on the location of buildings and offices for the visitors. Moreover, results showed that the application received a high acceptability rating and in fact is needed by the users.","PeriodicalId":34742,"journal":{"name":"Ho Chi Minh City Open University Journal of Science Engineering and Technology","volume":"43 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90685357","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A review of Khmer word segmentation and part-of-speech tagging and an experimental study using bidirectional long short-term memory 高棉语分词、词性标注及其双向长短期记忆实验研究综述

Ho Chi Minh City Open University Journal of Science Engineering and Technology Pub Date : 2022-04-20 DOI: 10.46223/hcmcoujs.tech.en.12.1.2219.2022

Sreyteav Sry, Amrudee Sukpan Nguyen

{"title":"A review of Khmer word segmentation and part-of-speech tagging and an experimental study using bidirectional long short-term memory","authors":"Sreyteav Sry, Amrudee Sukpan Nguyen","doi":"10.46223/hcmcoujs.tech.en.12.1.2219.2022","DOIUrl":"https://doi.org/10.46223/hcmcoujs.tech.en.12.1.2219.2022","url":null,"abstract":"Large contiguous blocks of unsegmented Khmer words can cause major problems for natural language processing applications such as machine translation, speech synthesis, information extraction, etc. Thus, word segmentation and part-of- speech tagging are two important prior tasks. Since the Khmer language does not always use explicit separators to split words, the definition of words is not a natural concept. Hence, tokenization and part-of-speech tagging of these languages are inseparable because the definition and principle of one task unavoidably affect the other. In this study, different approaches using in Khmer word segmentation and part-of-speech are reviewed and experimental study using a single long short-term memory network is described. Dataset from Asia Language Treebank is used to train and test the model. The preliminary experimental model achieved 95% accuracy rate. However, more testing to evaluate the model and compare it with different models is needed to conduct to select the more higher accuracy model.","PeriodicalId":34742,"journal":{"name":"Ho Chi Minh City Open University Journal of Science Engineering and Technology","volume":"73 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-04-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74641255","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0