Antioxidant activity of isolated compounds from ethyl acetate extract of Bruguiera parviflora

Abstract

Bruguiera parviflora belongs to Bruguiera genus, which possesses several biological activities such as antidiabetic, anti-inflammation, antioxidant. Phytochemical investigation of the ethyl acetate extract from Bruguiera parviflora leaves was carried out bioassay guided isolation and obtained three known compounds, namely kochioside A (1), oleanolic acid (2) and quercitrin (3). The chemical structure of isolated compounds were elucidated by spectroscopic data analysis as well as the comparison of their NMR data with the ones published in the literatures. All isolated compounds were evaluated for antioxidant activity with the free radical scavenging ability on 1,1-diphenyl-2-picrylhydrazyl (DPPH). Among them, quercitrin (3) was the most potent compound against DPPH with the IC50 value of 16.4 ± 0.2 g/mL, compared to vitamin C, which was used as positive control.