Novel Research in Microbiology Journal最新文献_第2页

Pseudomonas syringae pv. tomato and severity of bacterial speck disease on tomato plants in Egypt 西红柿假单胞菌与埃及西红柿植株上细菌性斑点病的严重程度

Novel Research in Microbiology Journal Pub Date : 2023-11-01 DOI: 10.21608/nrmj.2023.326365

Mahmoud Abou-Alhmd Soliman

{"title":"Pseudomonas syringae pv. tomato and severity of bacterial speck disease on tomato plants in Egypt","authors":"Mahmoud Abou-Alhmd Soliman","doi":"10.21608/nrmj.2023.326365","DOIUrl":"https://doi.org/10.21608/nrmj.2023.326365","url":null,"abstract":"Tomato is one of the most widespread vegetable crops in Egypt, which has a wide variation of cultivars. Tomato bacterial speck caused by Pseudomonas syringae pv. tomato is one of the most important tomato bacterial diseases. This study aimed to isolating and identifying P. syringe pv. tomato from infected tomato plants in Egypt, and investigate the tomato plants reaction towards the Egyptian bacterial strain, which the tomato cultivars or hybrids have low sensitivity to it. Currently, the Egyptian tomato plants exhibited typical symptoms of tomato bacterial speck disease. The isolation and identification experiments resulted in the recovery of typical isolates of P. syringae pv. tomato . The pathogenicity of the bacterial isolate was confirmed by molecular identification through detection of coronatine production and 16S rDNA analysis. The obtained nucleotide sequence was deposited at GenBank with accession no. OQ117369.1 and designed as P. syringae pv. tomato strain Pst1-MAS. The tomato plants only produced the typical bacterial speck symptoms among the tested solanaceous and cruciferous plants. The tested tomato plants exhibited variation in disease severity against P. syringae pv. tomato strain Pst1-MAS using a lot of disease severity scales. The tomato cultivars VFN-8 and Peto 86 were sensitive to infection with severe symptoms, while the tomato cultivars Super Marmande and Edkawy expressed relatively low symptoms. According to the results of disease severity; depending on the mean of bacterial speck symptoms, the tested plants were divided into high; moderate, low sensitive, and resistant. The related defense enzyme activity was correlated inversely with the disease severity for some of the tested tomato plants.","PeriodicalId":34593,"journal":{"name":"Novel Research in Microbiology Journal","volume":"12 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139300875","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recent advances in vulvovaginal Candidiasis research: A narrative review 外阴阴道念珠菌病研究进展综述

Novel Research in Microbiology Journal Pub Date : 2023-11-01 DOI: 10.21608/nrmj.2023.325275

Debasmita Dubey, Gopal Krishna Purohit, Shakti Rath, Sushree Swagatika Subhadarshini, Rajashree Panigrahi

引用次数: 0

Microbial contamination of cosmetics and the pharmaceutical products, and their preservation strategies: A comprehensive review 化妆品和药品的微生物污染及其保存策略综述

Novel Research in Microbiology Journal Pub Date : 2023-09-14 DOI: 10.21608/nrmj.2023.317346

Souvik Roy, Saheli Majumder, Aniket Deb, Lopamudra Choudhury

{"title":"Microbial contamination of cosmetics and the pharmaceutical products, and their preservation strategies: A comprehensive review","authors":"Souvik Roy, Saheli Majumder, Aniket Deb, Lopamudra Choudhury","doi":"10.21608/nrmj.2023.317346","DOIUrl":"https://doi.org/10.21608/nrmj.2023.317346","url":null,"abstract":"A brief discussion of the numerous types of microbial contamination of the pharmaceutical and cosmetic items and their corresponding effects is attempted in this study. The pharmaceutical and cosmetic products are particularly vulnerable to microbial contamination, because they contain chemical compounds that encourage the microbial development. Contamination can potentially happen during production; storage, and/ or usage. These contaminants can cause a variety of unfavorable effects, including alteration of the consistency and appearance; phase separation, alteration or loss of activity, and even the emergence of toxicity in the contaminated items. Organizations such as the United States Food and Drug Administration (FDA) actively regulate the consumer safety by frequently recalling the potentially dangerous or contaminated products from the market. Therefore, to prevent microbial contamination and increase the shelf life of a product, a variety of preservatives are added to the final formulation. However, some of these preservatives may be toxic to the consumer as well. In this context, we have also reviewed the mechanisms of action of some of the most commonly used antimicrobial preservatives, including the organic acids; parabens, phenol, organomercurials, ethanol, chlorobutanol, benzalkonium chloride","PeriodicalId":34593,"journal":{"name":"Novel Research in Microbiology Journal","volume":"31 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134971668","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Novel approach to overcome the β-lactam resistant bacteria using an actinobacterial inhibitory protein 利用放线菌抑制蛋白克服β-内酰胺耐药菌的新方法

Novel Research in Microbiology Journal Pub Date : 2023-09-07 DOI: 10.21608/nrmj.2023.320397

Nada A. M. Abdelbary, Rawia F. Gamal, Waleed M. Abdulkhair, Sawsan Y. Elateek

引用次数: 0

Chronic infection: as an ecological model for emergence of the chronic obstructive pulmonary disease 慢性感染:作为慢性阻塞性肺疾病出现的生态模型

Novel Research in Microbiology Journal Pub Date : 2023-09-01 DOI: 10.21608/nrmj.2023.319451

Dmitriy V. Alekseev, Artem V. Lyamin, Karim A. Kayumov

{"title":"Chronic infection: as an ecological model for emergence of the chronic obstructive pulmonary disease","authors":"Dmitriy V. Alekseev, Artem V. Lyamin, Karim A. Kayumov","doi":"10.21608/nrmj.2023.319451","DOIUrl":"https://doi.org/10.21608/nrmj.2023.319451","url":null,"abstract":"Chronic infections; emerging as complications of some primary diseases, can hardly be considered as classical infectious processes. Such infections may be caused by the non-traditional pathogenic bacteria; as in the cases of cystic fibrosis; chronic obstructive pulmonary disease (COPD), inflammatory bowel diseases, etc. Emergence of these complications is caused by several disorders in the ecosystem; constituting the human body and its microbiota. It is reasonable to extrapolate some ecological principles of the bacterial community's assembly in the humans; as there is a wide variety of factors affecting this, which can be considered as ecological ones. The human microbiota is formed during changes of the different bacterial succession types; under the influence of stochastic and deterministic ecological processes, in addition to different types of the selection pressure. During certain diseases, such as COPD, the normal course of the bacterial community's formation is disrupted. It is associated with anatomical and physiological defects, which cause changes in the properties of the ecological niche; occupied by the communities ( i.e ., airways in the case of COPD). Altered niches can be occupied by the virulent microorganisms, which get resource advantage and form new stable community. The severity of such chronic infections is correlated with biological diversity in the communities, which tends to decrease in the more severe cases and in the exacerbations. The aim of this study was to create an ecological model of the chronic infections; through synthesizing the ecological and medical knowledge. This model, such as the COPD; may establish new approach to the chronic infections etiopathogenesis, which enables to get better understanding of the clinical course in these diseases and to propose more effective therapies in the future.","PeriodicalId":34593,"journal":{"name":"Novel Research in Microbiology Journal","volume":"93 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135690481","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The CRISPR/Cas system: Gene Editing by bacterial defense CRISPR/Cas系统:细菌防御的基因编辑

Novel Research in Microbiology Journal Pub Date : 2023-09-01 DOI: 10.21608/nrmj.2023.317036

Amira H. El-Ashry

{"title":"The CRISPR/Cas system: Gene Editing by bacterial defense","authors":"Amira H. El-Ashry","doi":"10.21608/nrmj.2023.317036","DOIUrl":"https://doi.org/10.21608/nrmj.2023.317036","url":null,"abstract":"The most precise, effective, and widely used tool for editing the genome is currently the clustered regularly interspaced short palindromic repeat (CRISPR), which represents the prokaryotes adaptive immune defense. The CRISPR/Cas-9 genome editing system relies on two key elements; mainly the guide RNA (gRNA) and CRISPR-associated ( Cas-9 ) proteins. A complementary base pair in the designed sgRNA allows it to recognize the target sequence in the gene of interest. Either the non-homologous end joining or the homology-directed repair can be used to repair the double-stranded breaks, which are created by Cas-9 nuclease at a position upstream from a protospacer adjacent motif. The modified genome-editing tool CRISPR/Cas-9 has numerous applications in the various fields, such as biotechnology and medicine. Moreover, it is being studied for cancer management; Human Immunodeficiency virus (HIV), and as a gene therapy for several genetic diseases, including cystic fibrosis; sickle cell disease, and Duchenne muscular dystrophy. However, immunogenicity; off-target effect, and efficient delivery systems withstand against its spread in the clinical applications until introducing an improvement. The aim of this review was to summarize how the various CRISPR systems work; their important medical applications, and their limitations.","PeriodicalId":34593,"journal":{"name":"Novel Research in Microbiology Journal","volume":"30 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134918548","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Antifungal activity of essential oils emulsions, their bioactive compounds, and biological control of Fusarium wilt of Majorana hortensis 精油乳剂及其生物活性化合物的抑菌活性及对小麦枯萎病的生物防治

Novel Research in Microbiology Journal Pub Date : 2023-09-01 DOI: 10.21608/nrmj.2023.318807

Ali A.A.M., Hassanin M.M.H., Abdel-Rahman T.F.M., Mergawy M.M.

{"title":"Antifungal activity of essential oils emulsions, their bioactive compounds, and biological control of Fusarium wilt of Majorana hortensis","authors":"Ali A.A.M., Hassanin M.M.H., Abdel-Rahman T.F.M., Mergawy M.M.","doi":"10.21608/nrmj.2023.318807","DOIUrl":"https://doi.org/10.21608/nrmj.2023.318807","url":null,"abstract":"This investigation was conducted to evaluate the activity of some essential oils emulsions and a biocide (Bio-Cure F) for controlling Fusarium wilt disease of marjoram ( Majorana hortensis ) caused by Fusarium oxysporum . The fungal filtrate of F. oxysporum isolated from the infected marjoram plants significantly decreased the marjoram seed germination and caused wilting of its seedlings. The essential oil emulsions of thyme; basil, and marjoram, inhibited the in vitro fungal growth of F. oxysporum , and significantly suppressed its sporulation and spore germination. However, thyme oil was the most effective one, which completely inhibited the fungal growth at a concentration of 2000 µg/ ml, and completely prevented its sporulation and spore germination. The tested essential oils were analysed for their bioactive components using Gas Chromatography (GC), which were recorded as follows: linalool and eugenol were detected in basil; terpinen-4-ol, β-phyllandrene, and sabinene in marjoram oil, and thymol, 1.8-cineole, ρ-cymene, and α-terpinene in thyme oil. In the greenhouse experiments, Actamyl 70 % wp (wettable powder) and Bio Cure F were the most effective treatments, which significantly decreased the disease incidence and increased the plant growth parameters, compared with the untreated control. On the other hand, all treatments increased the peroxidase and polyphenol oxidase antioxidant enzyme activities, compared with the control; however, Actamyl 70 % (wp) and Bio Cure F had the superiority; recording 1.27 and 1.23 (units/ ml enzyme) for peroxidase, and 0.57 and 0.51 (units/ ml enzyme) for polyphenol oxidase, respectively. The results of the present study indicated the strong antifungal potential of the thyme essential oil. Meanwhile, Bio-Cur F biocide can be effectively used as a potent biofungicide to controlling the marjoram wilt incited by F.","PeriodicalId":34593,"journal":{"name":"Novel Research in Microbiology Journal","volume":"375 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135690442","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Porphyromonas gingivalis bacterium as a risk factor for Alzheimer’s disease! 牙龈卟啉单胞菌是阿尔茨海默病的危险因素！

Novel Research in Microbiology Journal Pub Date : 2023-08-25 DOI: 10.21608/nrmj.2023.314535

Andrei V. Kozlov, Artem V. Lyamin, Aleksei A. Neilenko, Anna V. Yanchenko, Alena A. Ereshchenko

引用次数: 0

Antifouling activity of microorganisms associated with the marine organisms 海洋生物相关微生物的防污活性

Novel Research in Microbiology Journal Pub Date : 2023-07-27 DOI: 10.21608/nrmj.2023.309908

A. Suresh, G. Immanuel

{"title":"Antifouling activity of microorganisms associated with the marine organisms","authors":"A. Suresh, G. Immanuel","doi":"10.21608/nrmj.2023.309908","DOIUrl":"https://doi.org/10.21608/nrmj.2023.309908","url":null,"abstract":"Biofouling is an ongoing problem in the marine industries that requires significant financial resources for control and new cleaning procedures. There is a feasible and immediate need to produce ecologically compatible low-toxic and harmless antifouling compounds for the maritime companies and underwater equipment; since the usage of Tributyltin (TBT) based marine coatings was banned globally in 2008. In recent years; the marine natural products have emerged as one of the most potential forms of antifouling agents. Although the natural antifoulants made from marine species; especially sponges and corals, have gained importance because of their performance in field tests; however, the gathering of larger quantities of marine animals is not a feasible choice. Several recent researches revealed that the marine microorganisms associated with sponges; corals, ascidians, seaweeds, and sea grasses, serve as the primary sources of antifouling substances and exhibit antimicrobial; antibacterial, and antifouling activity. During the screening of antifouling compounds; these marine microorganisms displayed antifouling ability against the macro and micro-foulers. This review aimed to focus on the improvements in the antifouling abilities of the natural products derived from marine organism-associated microorganisms.","PeriodicalId":34593,"journal":{"name":"Novel Research in Microbiology Journal","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42956215","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microbial culturomics: The next generation culture for identification of the human gastrointestinal microbiota 微生物培养组学：鉴定人类胃肠道微生物群的下一代培养

Novel Research in Microbiology Journal Pub Date : 2023-07-01 DOI: 10.21608/nrmj.2023.309506

Noha Mostafa Mahmoud

{"title":"Microbial culturomics: The next generation culture for identification of the human gastrointestinal microbiota","authors":"Noha Mostafa Mahmoud","doi":"10.21608/nrmj.2023.309506","DOIUrl":"https://doi.org/10.21608/nrmj.2023.309506","url":null,"abstract":"The maintenance of human health and the development of disease are both significantly influenced by the gut microbiota. The development of omics technologies improves the understanding of the gut microbial ecosystem. Metagenomics has emphasized the diversity of the gut microbiome; however, it does not provide reliable insight on the dark matter of microorganisms or the minor populations. As a result of the rebirth of cultural techniques in microbiology; the field of \"culturomics\" is created to cultivate the unidentified bacteria that reside inside the human gut. In the 21 st century's discipline of clinical microbiology; microbial culturomics becomes a promising strategy that may be used to cultivate hundreds of novel microorganisms linked to human; thus, opening new insights on the host-microbial relationships. Novel taxa and species will be detected by optimizing the culture conditions; followed by quick identification using mass spectrometry or molecular next generation sequencing. Culturomics of the human gut microbiota can be used as a bactriotherapy for the inflammatory bowel diseases and the respiratory illnesses like COVID-19, and as an immunomodulatory agent for cancer therapy. Furthermore, culturomics is a big store for discovering new antibacterial agents and resistance genes. The aim of this review was to highlight the background; methodologies, and future use of culturomics to study the human gut microbiota.","PeriodicalId":34593,"journal":{"name":"Novel Research in Microbiology Journal","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45193152","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0