Visnyk of Dnipropetrovsk University. Biology, medicine最新文献

{"title":"Characterization of staphylococci and their role in pathology of children","authors":"M. A. Harуfulina, O. Voronkova, T. Shevchenko, A. І. Vіnnіkov","doi":"10.15421/021422","DOIUrl":"https://doi.org/10.15421/021422","url":null,"abstract":"The problem of human staphylococcal infection in the world has been considered. It is known that there are 27 species of staphylococci, 14 of them colonize the skin and mucous membranes of humans, and 3 of them may cause diseases. They are Staphylococcus aureus , S. epidermidis and S. saprophyticus . Staphylococci are causative agents of the large part of community-acquired and nosocomial bacteremia, pneumonia, infections of skin and soft tissues, bones and joints. Of all cases of nosocomial infections, S. aureus accounts for 31%. During the identification of 65 strains of bacteria isolated from people with respiratory tract lesions, it was found that 58.5% of cases were connected with presence of staphylococci. 86.8% of isolates identified belong to species S. aureus and 13.2% to S. saprophyticus . Study of pathogenicity factors manifestation revealed that all strains identified as S. aureus had plasmocoagulase; lipase and lecitinase were simultaneously detected in 78.3% of them, among S. saprophyticus strains in 20%. Presence of haemolysins was determined for 82.1% of strains of staphylococci. Ability to film formation was detected in 21 (63.6%) strains of S. aureus and 2 (40%) of S. saprophyticus strains. Given high frequency of carriers of staphylococci among healthy individuals, they should be considered as potentially pathogenic microorganisms and opportunistic pathogens. Due to their pathogenicity factors (adhesins, capsule, cell wall components, enzymes and toxins), staphylococci may cause diseases such as sepsis, meningitis, peritonitis, endocarditis, pneumonia, tonsillitis, and in young children they may result in serious Ritter illness (“babies scalded syndrome”), foodstuff infection and staphylococcal enterocolitis, in lactating women – mastitis, toxic shock syndrome. To solve the problem of staphylococcal infection prevention, it is necessary to use the following prophylaxis methods: to avoid decrease in immunity, to observe the rules of hygiene, sterility, disposability, disinfection, strict control of sanitary and epidemiological profile of healthcare institutions, to ensure monitoring of pregnant women, infants and early diagnostics and treatment of the inflammatory diseases at their initial stages. Furthermore, there is a need in monitoring of these bacteria spreading.","PeriodicalId":345307,"journal":{"name":"Visnyk of Dnipropetrovsk University. Biology, medicine","volume":"44 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2014-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127074567","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Autoimmune reaction against neurospecific proteins and life quality of patients with congenital heart failure","authors":"Serìâ Bìologìâ, H. M. Lisunets","doi":"10.15421/021412","DOIUrl":"https://doi.org/10.15421/021412","url":null,"abstract":"The results of experimental investigation of autoimmune reaction targeted against neirospecific protein antigens in patients with congenital heart failure are presented. It is shown that titer for autoanibodies to these patients’ brain proteins is significantly higher compared with healthy persons. Elevation of titer of these autoantibodies is correlated with the ability to solve tasks for revealing complex analogies and decrease of life quality. Therefore, worsening of life quality and development of cognitive deficit in patients with cardiovascular diseases might be associated with autoimmune reaction against specific proteins of nervous tissue cells. The results obtained demonstrate that generation of autoantibodies in patients with congenital heart failure is linked with the cognitive deficit. One of the most important causes of cognitive decline could be hypoxia state due to surgery intervention. Consequences of hypoxia, in their turn, are accompanied by chronic oxidative stress. Molecular and cell injuries induced by free radicals are known to be the widely spread cause of various pathologies. Further investigations for validation of diagnostic hallmarks for estimation of the origin of progression of the cognitive deficit in patients with congenital heart failure are needed. These studies will allow clarifying mechanisms of influence of hypoxia-induced injuries associated with the aggravation of oxidative stress in nervous tissue cells. Understanding of relationship between decline of cognitive function and pathologies of cardiovascular system is necessary for more precise clinical diagnostics of the early prevention of possible complications and proper treatment of patients.","PeriodicalId":345307,"journal":{"name":"Visnyk of Dnipropetrovsk University. Biology, medicine","volume":"2 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2014-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131506836","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of Kerch Bay environmental pollution using neuroglial proteins of ground fish","authors":"H. V. Sukharenko","doi":"10.15421/021413","DOIUrl":"https://doi.org/10.15421/021413","url":null,"abstract":"The modern ecology situation in waters of the Kerch Strait requires assessment of disturbances in biotopes and monitoring of the degree of impact of industrial pollutants on ecosystem. Deposit of oil products after the 2007 year ships’ accidents might have considerable impact on the water biocenosis area. The investigation of cytoskeleton marker of astrocytes glial fibrillary acidic protein (GFAP) in brain of the bullhead ( Neogobius fluviatilis ), which is the typical representative of the commercial ground fish of the Kerch Strait, has been carried out. The results of comparative analysis of GFAP content in the brain of fish from the Kerch Bay near-shore waters and fish from conditionally clear area of Vorskla river shows the reliable (2.18 times) increasing of GFAP in the area of industrial pollution. Rising GFAP content indicates the astrogliosis development as a result of metabolic disturbances which can be induced by higher content of oil products in the near-bottom biotopes of the Kerch Bay. Increase in lipid peroxidation level was observed in the brain of fish from the Kerch Bay. The results provided with regard to violations of the state of astrocyte cytoskeleton and oxidative stress in the brain of bullhead from the Kerch Bay prove the sublethal biology effect of industrial pollutants in hydrobionts from this area. Results of this investigation also indicate the necessity of continuous ecology monitoring and comprehensive study of hydrobiont populations in the industrial regions and ecological disaster zones.","PeriodicalId":345307,"journal":{"name":"Visnyk of Dnipropetrovsk University. Biology, medicine","volume":"29 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2014-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115188175","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Activity of MMP2 and MMP9 matrix metalloproteinases in patients with myeloproliferative and lymphoproliferative diseases","authors":"I. Gordiienko","doi":"10.15421/021411","DOIUrl":"https://doi.org/10.15421/021411","url":null,"abstract":"MMP2 and MMP9 matrix metalloproteinases due to their ability to destroy basement membranes collagen and remodeling extracellular matrix (ECM) in the micro-environment of blood progenitor cells in the bone marrow play the important role in hematopoiesis. Displacement of normal hematopoiesis and dissemination of malignant cells in proliferative diseases of blood is also accompanied by catalytic ECM rearrangement. However, it is not known exactly how activity of MMP2 and MMP9 changes in various forms of leukemia and how it is affected by chemotherapeutic drugs. The aim of this study was to determine the influence of anthracycline antibiotics (daunorubicin and adriablastin) on MMP2 and MMP9 activity in blood plasma of patients with acute myeloid leukemia, chronic lymphocytic leukemia and multiple myeloma. It was established that proMMP9 activity was significantly reduced (0,03 ± 0,01 rel. u.) in patients with acute myeloid leukemia before the treatment, however, after chemotherapy, it increased approximately 7 times. Chronic lymphocytic leukemia and multiple myeloma were accompanied by significant increase of MMP9 activity. Application of daunorubicin led to decrease of proMMP9 activity (0,25 ± 0,10 rel. u.) in patients with chronic lymphocytic leukemia. ProMMP9 activity was significantly reduced (16 times) and that of MMP9 increased in case of multiple myeloma. In studying of MMP2 activity it did not significantly change. The conclusion is that the ratio of proMMP9/MMP9 can be used as the additional criterion for monitoring the effectiveness of chemotherapy of proliferative diseases of blood.","PeriodicalId":345307,"journal":{"name":"Visnyk of Dnipropetrovsk University. Biology, medicine","volume":"6 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2014-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126687311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hyperthyreosis effects on the learning and glial intermediate filaments of rat brain","authors":"S. Kyrychenko, V. Nedzvetsky","doi":"10.15421/021414","DOIUrl":"https://doi.org/10.15421/021414","url":null,"abstract":"The influence of hyperthyreosis on oxidative stress, state of glial intermediate filaments and memotry was investigated. Significant increasing of lipid peroxidation products into both hippocampus and cortex and change for the worse of memory was observed. Analysis of the behavioral reactions of rats in the test of passive avoidance conditioned reflex showed that the acquisition of skills of all groups of animals did not differ by time waiting period (latent period). Time saving memory test conditioned reflex of passive avoidance was excellent in the group of rats treated with thyroxine compared with controls. The change of polypeptide GFAP was observed in hippocampus and cortex. Both soluble and filamentous forms of GFAP increased in hippocampus of rat with hyperthyreosis. In filament fractions, increase in the intensity of 49 kDa polypeptide band was found. In the same fraction of insoluble cytoskeleton proteins degraded HFKB polypeptides with molecular weight in the region of 46 – 41 kDa appeared. Marked increase of degraded polypeptides was found in the soluble fraction of the brain stem. The intensity of the intact polypeptide ( 49 kDa ) , as well as in the filament fraction, significantly increased. It is possible that increasing concentrations of soluble subunits glial filaments may be due to dissociation of own filaments during the reorganization of cytoskeleton structures. Given the results of Western blotting for filament fraction, increased content of soluble intact 49 kDa polypeptide is primarily the result of increased expression of HFKB and only partly due to redistribution of existing filament structures. Calculation and analysis of indicators showed high correlation between the increase in content and peroxidation products of HFKB. These results indicate the important role of oxidative stress in the induction of astroglial reactive response under conditions of hyperthyroidism. This data shows the possibility of the glial cell cytoskeleton reconstruction under effect of thyroid hormones.","PeriodicalId":345307,"journal":{"name":"Visnyk of Dnipropetrovsk University. Biology, medicine","volume":"8 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2014-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124156241","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Фенотипическая пластичность в соотношении полов у Idotea baltica basteri (Crustacea, Isopoda) в Одесском заливе Черного моря","authors":"A. Y. Varigin","doi":"10.15421/021410","DOIUrl":"https://doi.org/10.15421/021410","url":null,"abstract":"The degree of phenotypic plasticity in the Idotea baltica basteri Audouin, 1827 (Crustacea, Isopoda) sex ratio of the fouling community in the Odessa bay of the Black Sea was determined. The ratio of males and females in three main phenotypes of I. baltica basteri , namely: uniformis, albafusca and lineata was found. Crustaceans were collected on the underwater surface of traverses, located in three sea districts with the different degrees of water exchange intensity with the open sea. Water depth at the wall of traverses ranged from 1 to2,5 m. In the first most opened district among the individuals of uniformis phenotype the ratio of males and females was equal to 1:4, that for albafusca – 1:1,3 and lineata – 1:1. In the second area with the release of drainage waters among the individuals of uniformis phenotype this ratio was equal to 1:4,5, while the albafusca and lineata figures were 1:1,5 and 1:1, accordingly. In the third region, with the most difficult water exchange, among the individuals of phenotypes the ratios of males and females were as follows: uniformis – 1:8, albafusca – 1:2, and lineata – 1:1. When moving from the opened to semi-enclosed area among the individuals of all phenotypes there was a gradual decline in the proportion of males and accordingly, increases of the proportion of females. The greatest number of males in all areas under study is observed among the individuals of lineata phenotype, and that of females – among the individuals of uniformis phenotype. It is found that monochromatic colored females prefer to stay in the shaded places among the seagrass beds and brightly colored males usually move actively along the outside of the substrata.","PeriodicalId":345307,"journal":{"name":"Visnyk of Dnipropetrovsk University. Biology, medicine","volume":"128 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2014-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126800002","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Oxidation process intensity in microsomal fraction of rat liver under conditions of different supplementation with polyunsaturated fatty acids","authors":"O. Ketsa, M. V. Zazulyk, M. Himchak","doi":"10.15421/021403","DOIUrl":"https://doi.org/10.15421/021403","url":null,"abstract":"The effect of fat compositions with the varying ratio of polyunsaturated fatty acids (PUFAs) of families ω-3 and ω-6 on oxidation process intensity in microsomal fraction of rat liver has been investigated. The aim of the study was to investigate the level of markers of oxidative modification of lipids and proteins in microsomal fraction of rat liver. Fat components in the experiment diets were presented by sunflower oil, soybean oil and fish oil. Rats were fed using one of the fillowing 5 diets for the period of 4 weeks: 1) AIN-93 diet with 7% sunflower oil and fish oil, with the inclusion of linoleic acid, eicosapentaenoic acid and docosahexaenoic acid in the ratio of ω-6:ω-3 – 7:1 (control diet); 2) AIN-93 diet with 7% soybean oil, with the inclusion of linoleic acid and α-linolenic acid in the ratio of 7:1; 3) the diet containing only ω-6 PUFAs; 4) the diet containing only ω-3 PUFAs; 5) the diet without PUFAs. The fatty acid compositions of the diets were analysed by gas chromatography. We measured the primary and secondary lipoperoxidation products, proteins carbonyl derivatives and SH -groups of proteins. It was shown that inclusion of linoleic acid and α-linolenic acid in the ratio of 7:1 or ω-6 PUFAs into the animal diet increased lipid peroxidation in microsomal fraction of the rat liver as compared with the control group. Only ω-6 PUFAs increased the oxidative modification of proteins in microsomal fraction of the rat liver as compared with the control rat group. High dose of ω-3 PUFAs – eicosapentaenoic acid and docosahexaenoic acid had no influence on free radical oxidation of lipids and proteins. Using the diet without PUFAs increased oxidation process intensity in microsomal fraction of rat liver. According to our study, ω-6 PUFAs increased the oxidative modification of lipids and proteins in microsomal fraction of the rat liver. ω-3 PUFAs, in particular, eicosapentaenoic acid and docosahexaenoic acid, increased lipid and protein resistance to oxidative modification in microsomal fraction of the rat liver.","PeriodicalId":345307,"journal":{"name":"Visnyk of Dnipropetrovsk University. Biology, medicine","volume":"12 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2014-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116754278","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of cytotoxic action of antihistamines desloratadine and loratadine using bulls spermatozoa as a test object","authors":"O. Kuzminov, D. Ostapiv, T. Alekhina","doi":"10.15421/021401","DOIUrl":"https://doi.org/10.15421/021401","url":null,"abstract":"Antihistamines with active ingredients of loratadine and desloratadine are produced by Ukrainian pharmaceutical industry. According to the law, ther are assessed for their potential danger to human health and the environment, including alternative test objects. Evaluation has been carried out with regard to cytotoxic effect of pharmacological substances (loratadine and desloratadine) using the bull sperm suspension as test objects, standardized and highly sensitive to toxic substances. Sperm was divided into the control sample (dissolved by phosphate-buffered saline) and the investigated sample. Loratadine was added to phosphate-buffered saline in doses of 1/500 LD 50 (12.3 mg), 1/100 LD 50 (61.5 mg) and LD 50 (6150 mg). Desloratadine doses were 1/500 LD 50 (1.25 mg), 1/100 LD 50 (6.25 mg) and LD 50 (625 mg). Survival of spermatozoa was defined until termination of rectilinear forward movement in sperm intacted at +2…+5 °C. Respiratory activity ( ex tempore ) was defined in 1.0 ml thermostated cell (temperature of 38.5 °C) by polarography with the automatic registration of process flow by potentiometer; restorative activity was defined potentiometrically, using the open microelectrodes that were inserted in thermostated polarographic cell. Survival of spermatozoa in the sperm under the impact of loratadine in doses of 1/500 LD 50 and 1/100 LD 50 is respectively 136.0 ± 26.2 hours and 144.0 ± 19.6 hours. Adding LD 50 dose of loratadine reduced survival to 112.0 ± 26.2 hours, which is lower than the control (160.0 ± 26.1 hours), respectively, by 10.0–15.0 and 30.0%. Loratadine reduces the respiratory activity of sperm: in the dose of 1/500 LD 50 by 20.5%, in the dose of 1/100 LD 50 – by 43.6%, and that of 100 LD 50 – by 61.5% compared to the control. Restorative sperm activity under the impact of the loratadine reduced by 84.0% (dose of 1/500 LD 50 ), 98.0% (dose of 1/100 LD 50 ), 80.0% (dose LD 50 ) compared to controls. The survival of spermatozoa in the sperm under desloratadine action in the dose of 1/500 LD 50 is 144.0 ± 19.6 hours (10.0% reduction compared with the control – 152.0 ± 23.6 hours), in the dose of 1/100 LD 50 – 136.0 ± 26.1 hours (15.0% reduction), in the dose of LD 50 – 72.0 ± 19.6 hours (30.0% reduction). The intensity of oxygen consumption by sperm reduced by 16.2% when adding desloratadine in the dose of 1/500 LD 50 , by 38.8% in the dose of 1/100 LD 50 and by 80.7% – in the dose of LD 50 , compared with the control. Inhibition of respiratory activity is accompanied by decreasing in the transport of protons and electrons from spermatozoa into the extracellular medium, that manifests decreasing of reconstructive activity when the dose of desloratadine is 1/500 LD 50 (by 12.0%), 1/100 LD 50 (by 40.0%) and LD 50 (by 60,0%) compared with the control.","PeriodicalId":345307,"journal":{"name":"Visnyk of Dnipropetrovsk University. Biology, medicine","volume":"5 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2014-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131031769","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Colonization of rhizoplane of cucumber roots by microorganisms which are components of the microbial preparation “Embiko®”","authors":"V. Rzhevskaya, L. Teplitskaya, I. P. Oturina","doi":"10.15421/021311","DOIUrl":"https://doi.org/10.15421/021311","url":null,"abstract":"Исследовано взаимодействие молочнокислых бактерий ( Lactobacillus plantarum, L. casei, Lactococcus lactis) и дрожжевых грибов Saccharomyces cerevisiae , входящих в состав микробиологического препарата «Эмбико®», с корнями растений огурца (Cucumis  sativus L.) сортов Конкурент и Феникс плюс in vitro. Изолированные штаммы исследованных микроорганизмов образуют вокруг корней облако колонии, различное по мутности и размерам. Штаммы L. plantarum и L. casei 6 образовывали однородное облако мелких колоний, одинаковое по диаметру на всех зонах корня и постепенно уменьшающееся в зоне корневого чехлика. Штамм S. cerevisiae  образовывал плотное облако крупных колоний, отличающееся по диаметру в разных зонах корня. Наименьшая интенсивность роста микроорганизмов наблюдалась на верхушке корня, наибольшая ˗˗ в зоне корневых волосков. Штамм молочнокислого стрептококка Lactococcus lactis  4/6 колонизирующей способностью не обладает. При инокуляциикорней микроорганизмами, ассоциированными в препарате «Эмбико®», колонизация корней происходит более активно, что свидетельствует о нормировании синергических взаимоотношений между лактобациллами и сахаромицетами при совместном их культивировании. Облако колоний, образованное микробиологическим препаратом «Эмбико®», выглядело неоднородным, четко просматривались колонии разного размера и цвета. Активная микробная колонизация всех зон корней огурцов связана с потреблением исследуемыми микроорганизмами корневых экзометаболитов в качестве источников энергии и углерода. Сортовая специфика огурцов не оказывает существенного влияния на ход процесса колонизации корней. Полученные результаты дают возможность охарактеризовать микроорганизмы, входящие в состав микробиологического препарата «Эмбико®», как способные колонизировать ризоплану корней растений огурца.","PeriodicalId":345307,"journal":{"name":"Visnyk of Dnipropetrovsk University. Biology, medicine","volume":"58 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2013-12-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116524029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microbiocenoses of Lviv sewage at various stages of purification","authors":"K. V. Sholiak, S. Hnatush, T. Peretyatko, S. Gudz","doi":"10.15421/021313","DOIUrl":"https://doi.org/10.15421/021313","url":null,"abstract":"Досліджено мікробоценози стічних вод міста Львів на різних етапах очищення. Показано якісний та кількісний склад мікроорганізмів первинного та вторинного відстійників, аеротенка та активного мулу. Охарактеризовано закономірності поширення мікроорганізмів різних фізіологічних груп на різних етапах очищення. Встановлено співвідношення мікроорганізмів різних фізіологічних груп на певному етапі очищення стічних вод. У первинному відстійнику за чисельністю переважали нітрифікувальні (7,1 × 106КУО), азотфіксувальні бактерії (9,0 × 106КУО), а також гриби (3,4 × 106КУО). В аеротенку чисельність мікроорганізмів зростала, однак їх видовий та відсотковий склад не змінювався. В активному мулі виявлено зростання відсотка мікроорганізмів, що використовують мінеральні форми нітрогену. Визначено відсотковий вміст хромрезистентних штамів серед представників різних фізіологічних груп. Ці мікроорганізми можуть бути перспективними для розробки біотехнологічних методів очищення стічних вод від сполук хрому, які є високотоксичними для живих організмів.","PeriodicalId":345307,"journal":{"name":"Visnyk of Dnipropetrovsk University. Biology, medicine","volume":"50 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2013-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122482576","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}