Visnyk of Dnipropetrovsk University. Biology, medicine最新文献

{"title":"Action of manganese salt on Triticum aestivum","authors":"E. Tkach, M. Vakerich, V. Nikolaychuk","doi":"10.15421/021423","DOIUrl":"https://doi.org/10.15421/021423","url":null,"abstract":"Залежно від концентрації важкі метали не тільки токсичні, а навпаки, можуть бути необхідними елементами для нормального росту та розвитку рослин. Одним із таких металів, який одночасно виступає як мікроелемент, є манган (марганець). Специфічна потреба у марганці властива для всіх рослин. Доведено участь даного елемента у перебігу низки фізіолого-біохімічних процесів у рослинному організмі. Манган у рослині відіграє важливу роль в окислювально-відновних реакціях, виступає як сильний окисник, як кофактор, є необхідною складовою багатьох ферментів тощо. Нами досліджено вплив передпосівної обробки водним розчином сульфату марганцю різних концентрацій на розвиток насіння озимої пшениці сорту Артеміда. У ході роботи спостерігали як інгібувальну, так і стимулювальну дію сірчанокислого мангану на ріст і розвиток культури, залежно від концентрації. Вивчали такі ростові процеси: схожість насіння, висота проростка, вага рослин та їх кореневих систем. Оптимальна концентрація сульфату марганцю для передпосівної обробки насіння – 0,001–0,01%. Критичною, за якої спостерігається фітотоксична дія (пригнічення росту, зменшення біомаси проростка), є концентрація 0,5–1,0% розчину сульфату марганцю для передпосівної обробки насіння.","PeriodicalId":345307,"journal":{"name":"Visnyk of Dnipropetrovsk University. Biology, medicine","volume":"5 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2014-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128949905","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correlation between aerobic and anaerobic microflora of the urogenital tract at dysbiosis in women of Dnipropetrovsk city","authors":"A. H. Yaderna, L. P. Golodok, A. Vinnikov","doi":"10.15421/021421","DOIUrl":"https://doi.org/10.15421/021421","url":null,"abstract":"Методом полімеразної ланцюгової реакції (ПЛР) у режимі реального часу досліджено кількісні характеристики нормальної та умовно-патогенної аеробної / факультативно-анаеробної, анаеробної біоти в уретрі, цервікальному каналі та піхві здорових дівчат і жінок віком 10–40 років. У всіх жінок до 40 років і частини жінок віком понад 40 років біота представлена переважно лактобацилами. У частини жінок після 40 років мікробний склад біоценозу характеризується зниженням кількості лактобацил і заміщенням їх анаеробними мікроорганізмами, головним чином Atopobium vaginae (16%), Gardnerella vaginalis (12%), Megasphaera spp. (8%), Dialister spp. (8%), Eubacterium spp. (8%) і Porphyromonas spp. (4%). У рідкісних випадках має значення також і великий спектр інших збудників, у тому числі патогенних: Trichomonas vaginalis (8%), Mycoplasma genitalium (4%), Neisseria gonorrhoeae (2%) і Chlamydia trachomatis (1%). Найчастіше зустрічалися асоціації штамів: A. vaginae та G. vaginalis , Eubacterium spp. та Porphyromonas spp. , U. ( urealyticum + parvum ) та M. genitalium , N. gonorrhoeae та Ch. trachomatis , U. ( urealyticum + parvum ) та Candida spp . Це пов’язано з анатомо-фізіологічними особливостями статевих органів, гормональним фоном і дією імунної системи. Кількісне дослідження біоти урогенітальго тракту жінок м. Дніпропетровськ за допомогою ПЛР у реальному часі – адекватний метод діагностики фізіологічних і патологічних змін, що дозволяє діагностувати дисбіотичні порушення на ранніх етапах і запобігти їх подальшому розвитку у складніші форми.","PeriodicalId":345307,"journal":{"name":"Visnyk of Dnipropetrovsk University. Biology, medicine","volume":"74 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2014-07-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124363975","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Role of Hp system in adaptation of specific immunity indices to the influence of moderate physical activity","authors":"V. Sokolenko, S. Sokolenko","doi":"10.15421/021406","DOIUrl":"https://doi.org/10.15421/021406","url":null,"abstract":"The aim of this study is to determine the role of haptoglobin phenotype in realization of adaptive responses of cellular and humoral immunity indices to moderate exercise caused by physical training. The study was implemented in the group of second-year students aged 18–20 who lived in the same climatic and geographical conditions for a long period of time. The students didn’t have any acute or chronic diseases and attended the main group of physical training. 60 persons were investigated. Immune system indices analysis was carried out in September before and after physical training lessons. Leukocyte level was calculated using hemocytometer, lymphocyte level was determined on the base of blood smear (dyeing for Romanowsky–Giemsa). Expression of surface antigene by peripheral blood lymphocyte was determined by immuno-fluorescence method with the use of monoclonal antibodies. The level of immunoglobulin in plasma was determined by radial immunodiffusion or Mancini method. To assess the phenotype of haptoglobin (Hp) we used the method of electrophoresis in starch gels. In the course of research we have detected the reduction of the relative and total number of lymphocytes regardless of haptoglobin phenotype in the group of students after physical training; this is a typical feature of the initial stages of stress response. We observed statistically reliable decrease in total number of analyzed subpopulations of T-lymphocyte in the group of students with phenotype Hp2-2 which was obviously the result of changes in the general level of lymphocytes in the peripheral blood. In the group of students with phenotype Hp1-1 absolute number of T-lymphocyte with phenotype CD3+ and CD4+ is reduced. In the group of students with phenotype Hp2-1 we have seen only the tendency to decrease in functional mature T-lymphocyte and their helper subpopulation. In the group of students with phenotype Hp2-2 the relative number of helper T-lymphocyte with the phenotype of CD4+ was reduced after moderate physical activity and it caused the decline in immune regulating index CD4+/CD8+. Changes of the level of B-lymphocyte with CD72+ phenotype were not found regardless of the haptoglobin phenotype. Analysis of the impact of moderate physical activity on major classes of serum immunoglobulin revealed the reduction of IgG level in the group of students with phenotype Hp2-2. Thus, we draw the conclusion that serum haptoglobin system shares the adaptive reactions of immune system to moderate physical activity. The most pressing phenotype is Hp2-2. Even in the group of students with immune pressing haptoglobin phenotypes, the analyzed parameters do not go beyond the homeostatic regulations after physical activity and it indicates satisfactory level of immune system adaptation to performed physical activity.","PeriodicalId":345307,"journal":{"name":"Visnyk of Dnipropetrovsk University. Biology, medicine","volume":"20 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2014-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114374416","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Влияние цефтриаксона и тетрациклина на формирование биопленки штаммами Staphylococcus epidermidis","authors":"O. I. Sidashenko, O. S. Voronkova, Y. A. Sirokvasha, A. I. Vinnikov","doi":"10.15421/021402","DOIUrl":"https://doi.org/10.15421/021402","url":null,"abstract":"122 strains of staphylococci were identified. Among the examined 122 clinical strains of staphylococci, 67 strains belonged to coagulase-positive, and 55 strains to the coagulase-negative ones. According to the study of physiological and biochemical properties, it was found that 37 strains (30.3%) belonged to S. epidermidis species. One of the biological properties of many bacteria is the ability to film formation and these strains attract special attention, since it is known that the film antibiotic resistance is higher than in planktonic cultures. It was determined that 20 strains of those under study were film-forming, 17 strains – non-biofilm forming ones. The film was formed during three days, and settled to the bottom of the plate holes. The clinical (Cl) strain of S. epidermidis was sensitive to ceftriaxone and tetracicline. The control (C) strains of S. epidermidis were sensitive to ceftriaxone, tetracycline and sizomicine. The study of biofilm growth for 2, 3 and 4 days of incubation was carried out. The maximum rate of biofilm S. epidermidis C was observed during 2–3 days; there is the most intense increase of cells number from 5.2 × 10 8 CFU/ml, for S. epidermidis Cl to 5.6 × 10 8 CFU/ml. The effect of ceftriaxone and tetracycline on biofilm formation by 2 investigation strains of S. epidermidis was found. We determined differences in minimal inhibitory concentrations (MIC) for planktonic cultures and biofilm of strains under study. It was established that MIC antibiotics inhibited the growth of planktonic cultures on average 2 times lower compared to the MIC which inhibited the biofilm formation. MIC for planktonic culture of S. epidermidis Cl defined for ceftriaxone was equal to 10 mg/ml, and for tetracycline – 1 mg/ml. MIC of ceftriaxone for the control strain was equal to 12 mg/ml, MIC of tetracycline – 0.7 mg/ml. MIC values for dynamics biofilm formation of S. epidermidis Cl strain on the plater were as follows: to ceftriaxone – 20 mg/ml and for tetracycline – 2 mg/ml, MIC of ceftriaxone for S. epidermidis C strain – 24 mg/ml, MIC of tetracycline – 1.5 mg/ml. The effect of ceftriaxone and tetracycline was defined to the larger extent, than MIC for biofilm-forming on the plate (10, 50 and 100 times). More effective action of tetracycline was shown for 1- and 2-daily biofilm cultures of S. epidermidis clinical strain. Adding tetracycline concentration of 20 mg/ml in the culture medium of the 1-day biofilm of S. epidermidis Cl strain reduced the cell number of biofilm formation 590 times, increasing concentrations of tetracycline to 100 mg/ml and added to the 1-day biofilm of the clinical strain reduced the number of bacterial cell 4400 times compared with control.","PeriodicalId":345307,"journal":{"name":"Visnyk of Dnipropetrovsk University. Biology, medicine","volume":"53 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2014-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126643146","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Features of the gastric periodic activity in conditions of NO-ergic system disbalance","authors":"O. Severynovska, O. O. Galinskij, A. Rudenko, O. B. Mursin, V. Babicheva, L. D. Skubytska","doi":"10.15421/021415","DOIUrl":"https://doi.org/10.15421/021415","url":null,"abstract":"Результатом тривалого уведення донатора NO є перебудова періодичної моторної та секреторної активності шлунка. Зменшувались показники моторного індексу на 6-ту добу, суттєво (на 56%) знижувалась міоелектрична активність шлунка на 12-ту добу. Зміни секреторної функції шлунка проявлялися у достовірному зменшенні об’єму виділення шлункового соку в 2,0 та 3,3 раза, зростанні рН , зниженні концентрації глікопротеїнів на 57 та 39%, збільшенні концентрації пепсину в 1,8 та 2,6 раза на 6- та 12-ту добу відповідно. За цих змін спостерігали розбалансування механізмів регуляції секреторної активності шлунка, а вплив натрію нітропрусиду на головні та поверхневі епітеліальні клітини слизової оболонки шлунка був вираженіший у разі збільшення тривалості дії чинника. При уведенні блокатора синтезу NO – L-NNA порушувалась фазова картина міоелектричної активності (МЕА) шлунка, яка ставала подібною до переходу між ІІ та ІІІ фазами. Показники міоелектричного імпульсу (МІ) підвищувались в 1,3 раза на 6-ту добу уведення блокатора. На 12-ту добу спостерігали зміну характеру скорочень у всіх фазах МЕА шлунка та зниження МІ на 28%. Перебудови секреторної функції шлунка проявлялися достовірним збільшенням об’єму шлункового соку на 61 та 17%, у значному зростанні його рН на 6-та 12-ту добу відповідно. Встановлено порушення кислототвірної функції шлунка та активності пілоричного сфінктера, спричинене закиданням дуоденального вмісту до шлунка.","PeriodicalId":345307,"journal":{"name":"Visnyk of Dnipropetrovsk University. Biology, medicine","volume":"83 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2014-04-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123205204","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Мonitoring of viruses spread among domestic cats and dogs in Dnipropetrovsk","authors":"L. V. Yashchuk, N. Cherevach, A. Vinnikov","doi":"10.15421/021405","DOIUrl":"https://doi.org/10.15421/021405","url":null,"abstract":"Cats and dogs kept at home, as well as other animals, are susceptible to infectious diseases caused by pathogenic microorganisms, including viruses. Viral infections in urban environments are extremely common and cause severe diseases in domestic animals which often lead to death, resulting in high material and moral damages to owners of animals. Therefore, investigation of the prevalence of pathogens of viral diseases is very important in our time. The aim of this work is to define the indicators of spreading viral diseases of animals in Dnipropetrovsk, to analyze the seasonal spread of viral infections in animals, and influence of sex and breed on the frequency of disease manifestations. Materials for research were obtained on the basis of three veterinary clinics in Dnipropetrovsk. During the study, we used serological methods of diagnostics of clinical materials, namely ELISA and IHA. Immunosorbent assay was performed using the thermostatic shaker ST-3 and strip immunoassay analyzer Stat Fax 303 Plus. Also we used commercial test systems VetExpert CAV Ag and Feline VacciCheck ImmunoComb ® , based on the immuno-chromatographic method. During clinical studies of 491 animals (268 cats and 223 dogs) it was found that the most common respiratory viral infections in cats were calicivirus infection and rhinotracheitis, while in dogs there were viral diseases of gastrointestinal tract, i.e. enteritis and hepatitis. Using IHA method, we revealed the antibodies to respiratory viruses in the blood of deseased cats: to calicivirus – in 95 cats, to rhinotracheitis – in 60 cats; by ELISA method we identified antigens of parvovirus, enteritis pathogen, in biological material of 61 dogs, by IHA we found antibodies to virus of infectious hepatitis in 49 individuals. Based on these data, it has been revealed that during the winter months the animals suffered mostly the respiratory viral infections (60%), and in spring there were increased occur-rence of enteritis, observed in all age groups of animals. From May, hepatitis started to appear and prevailed for all summer months, mixed with enteritis (about 50% of total number of cases). Hepatitis was recorded both in cats and dogs. By the fall, recurrence was observed for calicivirus and herpes virus infections in cats, and the cases of enteritis decreased, while hepatitis was recorded until mid-October. Such statistics is kept relatively constant. The research results can be applied in the practice of veterinary laboratories for the development of diagnostic measures and prevention of viral diseases in domestic animals, as well as in the prediction of dissemination of viral infections in animals in the near future.","PeriodicalId":345307,"journal":{"name":"Visnyk of Dnipropetrovsk University. Biology, medicine","volume":"21 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2014-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121947540","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Frequency of urogenital Mycoplasma detection in women of Dnipropetrovsk","authors":"K. V. Bubalo, L. P. Golodok, A. Vinnikov","doi":"10.15421/021409","DOIUrl":"https://doi.org/10.15421/021409","url":null,"abstract":"The frequency of urogenital mycoplasmas detection in women of different ages was studied in culture with the help of DUO test-system in order to determine their etiological significance in the development of inflammatory processes of women urogenital tract. We identified the researched cultures Mycoplasma hominis , Ureaplasma urealyticum in the diagnostic titer >10 4 TEM/ml indicating severe contamination by microorganisms, and in the titer 10 4 TEM/ml, 10 4 TEM/ml was observed in 55 women (46%) and 20 women (17%), respectively, and the titer of <10 3 CFU/ml U. urealyticum was observed in 20 women (17%), and M. hominis in 18 women (15%). Analysis of genital mycoplasmas distribution among women of different ages has shown that there was the certain correlation between the patient age and frequency of genital mycoplasmas detection: the highest detection rate was observed in women age of 24–29. The dominant pathogen of urogenital tract inflammatory processes in women in 24–29 age group is U. urealyticum . The comparison of DUO test-system and PCR data has shown that DUO test-system in culture allowed more sensitive quantitave characterization of mycoplasmas, however, for the more effective laboratory diagnostics it was necessary to use complex methods to increase the probability of pathogen detection. Incidence of mycoplasmas in women with the presence of inflammation was higher than in women having the inflammation in the genital tract. In this case, potential symptom-free carriers exist for the development of inflammation of urogenital tract of women. Scientists have proved that mycoplasma could cause vulvovaginitis, urethritis, paraurethritis, bartholinitis, adnexitis, salpingitis, endometritis, and ovaritis.","PeriodicalId":345307,"journal":{"name":"Visnyk of Dnipropetrovsk University. Biology, medicine","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2014-04-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129430359","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Preimplantation development of embryos in women of advanced maternal age","authors":"O. Chaplia, J. Gontar, N. Bilko","doi":"10.15421/021404","DOIUrl":"https://doi.org/10.15421/021404","url":null,"abstract":"In order to reveal the influence of genetic component on the early embryo development, the retrospective study of morphokinetic characteristics of 717 embryos subjected to preimplantation genetic testing was conducted. Blastomere biopsy for FISH-based preimplantation genetic screening of 7 chromosomes was performed on the third day of culture, while embryo developmental potential and morphological features at the cleavage and blastulation stage were studied regarding maternal age particularly in the group of younger women and patients older than 36. Results of genetic testing revealed that euploid embryos rate gradually decreased with maternal age comprising 39.9% in young women group and 25.3% of specimen belonging to elder patients. At the cleavage stage, morphological characteristics of aneuploid and euploid embryos didn’t differ significantly regardless of the age of patients that could be accounted for the transcriptional silence of embryo genome till the third day of its development. However, in case of prolonged culture chromosomally balanced embryos rarely faced developmental arrest (in 7.9%) and formed blastocysts half more frequently compared to aberrant embryos (respectively 75.6 versus 49.8%). Nevertheless, no substantial difference was found between blastocyst formation rate among embryos with similar genetic component regardless of the maternal age. Taking into consideration high rate of chromosomally unbalanced embryos specific to patients of advanced maternal age, the relative proportion of aneuplouid blastocysts was significantly higher in this group of embryos. Thus, without genetic screening there is a possibility of inaccurate selection of embryos for women of advanced reproductive age for transfer procedure even in case of prolonged culture. Consequently, increase of aneuploid embryos frequency associated with permanent preimplantation natural selection effectiveness along with the postimplantation natural selection failure may be the cause of elevated risk of chromosomally abnormal child birth for advanced age women after natural conception, as well as after assisted reproduction therapy.","PeriodicalId":345307,"journal":{"name":"Visnyk of Dnipropetrovsk University. Biology, medicine","volume":"5 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2014-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129240116","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mechanisms of antibiotic resistance of Enterobacteriaceae family representatives","authors":"K. R. Kotsyuba, O. Voronkova, A. І. Vіnnіkov, T. Shevchenko","doi":"10.15421/021407","DOIUrl":"https://doi.org/10.15421/021407","url":null,"abstract":"The paper deals with the basic medical scheme of antibiotics use for treatment of lesions caused by enterobacteria and mechanisms of resistance of Enterobacteriaceae to different classes of antibiotics. It is known that the main mechanisms of resistance to antibiotics are enzymatic inactivation, modification of the target, efflux, violation of conduct through the membrane and formation of metabolic shunt. The most common cases of resistance to beta-lactams among Enterobacteriaceae relate to production of plasmid and chromosomal beta-lactamases, violation of the permeability of the outer membrane, and modification of target penicillin binding proteins. Active release of antibiotics from the cell, or efflux, in Enterobacteriaceae is used for maintaining resistance to tetracyclines, macrolides, carbapenems. Genes of efflux system are localized on plasmids and contribute to rapid spreading among Enterobacteriaceae. Mutations are the basis of resistance to novobiocinum and rifampicinum. Enzymatic inactivation by modifying is typical for resistance to aminoglycosides. Three groups of enzymes are engaged in the process, by adding the molecule of acetic acid, phosphate or adenine. Joining of these groups is irreversible and leads to complete loss of biological activity of the antibiotic. Resistance to aminoglycosides appears also due to inhibition of drug penetration, that is associated with genetically determined mechanisms of electron transport through the membrane. Resistance to quinolones and fluoroquinolones is associated with the modification of topoisomerase II and IV which are targets of these groups of antibiotics. Resistance is possible as a result of changes in the structure of the target, breaching of penetration into the cell, and active release from the cell. The highest level of resistance is develope in the case of two- or three-stage mutations in one or the other, or both, subunits in different genes. At the same time, for breaching of the bacterial cell it is enough to suppress the activity of only one enzyme associated with different functions of both topoisomerases. Another mechanism of resistance to quinolones is the reduction of permeability through bacterial outer membrane, that it’s possible due to decreasing of permeability of porine channels. In this case, decreasing of permeability efficacy takes place not only for quinolones, but also for other classes of antibitics. In addition, reduced sensitivity to quinolones efflux may play the significant role. For clinical strains of microorganisms, cross-resistance to various drugs, associated with simultaneous production of various enzymes that inactivate antibiotics, is typical.","PeriodicalId":345307,"journal":{"name":"Visnyk of Dnipropetrovsk University. Biology, medicine","volume":"47 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2014-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126587176","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of quality of permanent teeth restorations in children of areas contaminated by heavy metal salts","authors":"V. Avakov, N. Rozhko","doi":"10.15421/021408","DOIUrl":"https://doi.org/10.15421/021408","url":null,"abstract":"The influence of the environment on the child health is one of priority issues of the present time and it is of great social importance. Increased dental diseases associated with climatic and geographical characteristics of the area are widely discussed in the literature. The leading among them are environmentally determined dental diseases in children associated with geochemical and technogenic pollution of the area where they live. Increasing amounts of hard metal salts is the urgent hygienic problem, due to severity of their multi-element analysis in microsubjects, and negative influence on health of the children population, due to tropism, ability to cumulation, long biological life in the body and antagonism of heavy metal salts to the number of microelements. Influence of hard metal salts on dental diseases development is undeniable. Particular attention is paid to their influence on caries process and treatment peculiarities. Despite the fact that modern аdhesive dentistry in recent years has made a significant breakthrough in improving adhesive systems, correct choice of adhesive system depending on changes in the structure of hard tissue under geochemical contaminants (like heavy metal salts) is the most important step. It is the decisive factor for adaptation and connection of restoration with the restoration base. We should remember that on the way of adhesive system there is an altered structure preventing from deep penetration of such system and, consequently, leading to violation of restoration tightness. Therefore, early detection of complications by clinical evaluation of quality of the restorations is of great interest. Multi-vector approach to treatment of dental caries in children living in conditions of technogenic pollution by heavy metal salts is extremely urgent and important issue. Significant niche in this approach is given to adhesive preparation methods combined with local fluoridation, using fluoride medication of the new generation. One of them is Hluftored which promotes the formation of microcrystalline СаF 2 . Protocol of work with this new generation drug is based on the sequential precoating of enamel with magnesium-fluoride silicate, and then with suspension of high desperasible calcium hydroxide promoting formation of microcrystalline СаF 2 , magnesium and copper with the size of ~50Аo, leading to easy penetration of fluoride into pores of softening range. Moreover, microcrystals of CaF 2 are protected by silicic acid gel from outwashing. Therefore, they release fluoride for a long period of time (over 1 year), which promotes remineralization and prevents complications of dental caries. This article includes the data regarding effectiveness of the Hluftored use in combination with various adhesive systems. Also, we show data of clinical quality assessment conducted for restoration procedures in children of different age groups and territories by using index of USPHS, after 6 and 18 months of carrying out such p","PeriodicalId":345307,"journal":{"name":"Visnyk of Dnipropetrovsk University. Biology, medicine","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2014-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125181192","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}