{"title":"Human's weight perception and load force characteristics in lifting objects with a power assist robot","authors":"S. Rahman, R. Ikeura, M. Nobe, H. Sawai","doi":"10.1109/MHS.2009.5351850","DOIUrl":"https://doi.org/10.1109/MHS.2009.5351850","url":null,"abstract":"This paper deals with the design of a 1DOF power assist robot for lifting objects. Human's vertical lifting force, comprises of inertial force and gravitational force, was considered as the desired dynamics of the robot system. We hypothesized that human's perception of object weight due to inertial force might be different from the perceived weight due to gravitational force for lifting object with the power assist robot system. We then simulated the system using MATLAB/SIMULINK and lifted different sizes of objects with the system. We analyzed human's perceived weights as well as load force characteristics for lifting objects with the system. The analyses show that human's perception of object weight is related to load force rates instead of load force magnitudes. The analyses clarify that the magnitude of the peak load force is to be optimized in order to optimize the motion of the power assist robot. On the other hand, load force rate is to be optimized in order to optimize the feeling of heaviness of object manipulated with the power assist robot. The results also introduce the technique of simultaneous optimization of motion and perceived heaviness. Finally, we proposed using the findings to design human-friendly power assist robots for carrying heavy objects in various industries.","PeriodicalId":344667,"journal":{"name":"2009 International Symposium on Micro-NanoMechatronics and Human Science","volume":"54 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2009-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134017636","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Chie Katsuda, K. Niiyama, Eriko Obana, Takenori Yamamoto, T. Matsuo, K. Ohkura, M. Kataoka, Y. Shinohara
{"title":"Specific formation of trypsin resistant micelle structure on a hydrophobic peptide observed with Triton X−100 but not with ocytlglucoside","authors":"Chie Katsuda, K. Niiyama, Eriko Obana, Takenori Yamamoto, T. Matsuo, K. Ohkura, M. Kataoka, Y. Shinohara","doi":"10.1109/MHS.2009.5351863","DOIUrl":"https://doi.org/10.1109/MHS.2009.5351863","url":null,"abstract":"Interaction manners of the coat peptide of Pf3 phage, Pf3 peptide, with lipid bilayer have been extensively studied. We designed a derivative of Pf3 peptide, referred to as DDRK peptide, and this peptide was subjected to trypsin digestion to understand its physicochemical properties. In the presence of Triton X−100 used for solubilization of DDRK peptide, trypsin digestion of DDRK peptide caused specific cleavage at its N-terminal Lysine residue. N-terminal region of the DDRK peptide is relatively hydrophilic, but its remaining region is hydrophobic. Thus, hydrophobic region of DDRK peptide is expected to be coated by Triton micelle, and formation of micelle structure of Triton seemed to cause selective cleavage of the DDRK peptide at its hydrophilic N-terminal Lys residue by trypsin. However, such protective effect on the DDRK peptide against trypsin digestion was not observed with octylglucoside. The observed results are important for understanding the interaction manners of detergents with hydrophobic peptides.","PeriodicalId":344667,"journal":{"name":"2009 International Symposium on Micro-NanoMechatronics and Human Science","volume":"737 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2009-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116110746","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Novel fluorescent probe for measurement of extracellular matrix degradation","authors":"T. Murai, H. Kawashima","doi":"10.1109/MHS.2009.5351906","DOIUrl":"https://doi.org/10.1109/MHS.2009.5351906","url":null,"abstract":"The invasive properties of cancer cells are affected by cell adhesion to the surrounding extracellular matrix. We previously reported that degradation of the major extracellular matrix component hyaluronan upregulates cancer cell migration. Although hyaluronan degradation to smaller fragments is a key reaction in regulating cancer progression, simple methods for continuously detecting hyaluronan-degrading activity have not been established. Here, we show that fluorescently-labeled hyaluronan serves as a substrate for continuous measurement of hyaluronan-degrading activity. The developed assay method is useful for investigation of the function of hyaluronan in cancer progression.","PeriodicalId":344667,"journal":{"name":"2009 International Symposium on Micro-NanoMechatronics and Human Science","volume":"22 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2009-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121076783","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Hybrid vision-force guided fault tolerant robotic assembly for electric connectors","authors":"P. Di, Jian Huang, Fei Chen, H. Sasaki, T. Fukuda","doi":"10.1109/MHS.2009.5352078","DOIUrl":"https://doi.org/10.1109/MHS.2009.5352078","url":null,"abstract":"To Detect and recover the errors occurred in the task of mating connectors is vital in robotic wiring harness systems. In our previous work, a set-membership approach for static piecewise affine (PWA) system was proposed. Although using the static force model, errors can be detected effectively during mating connectors, there are still some mistaken detections and unrecognized faults, due to the insufficient sensor information and limitation of model. Before the mating task, there are various kinds of grasping errors. Only using force sensor is unable to detect the grasping errors. In this study, a new hybrid vision-force guided fault tolerant approach is proposed to improve the rate of error detection. More features from the vision system are chosen as the parameters of the fault tolerant system. Multiple sensors including a force sensor, encoders and an industrial vision system are assumed to acquire the necessary information of the method. The effectiveness of these methods is finally confirmed through experiments.","PeriodicalId":344667,"journal":{"name":"2009 International Symposium on Micro-NanoMechatronics and Human Science","volume":"7 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2009-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123855290","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Functional analysis of a chromosomal passenger protein, Borealin, by multiphoton excitation-evoked chromophore-assisted laser inactivation","authors":"Y. Harada, P. Dai, T. Takamatsu","doi":"10.1109/MHS.2009.5352016","DOIUrl":"https://doi.org/10.1109/MHS.2009.5352016","url":null,"abstract":"Borealin, one of the chromosomal passenger proteins, is reported to play important roles in the mitotic process. To analyze precise functions of Borealin in the mitotic phase, we applied the method of multiphoton excitation-evoked chromophore-assisted laser inactivation. Although further studies are needed, our results suggest that Borealin is required for mitosis, as previously reported. These results provide insights into understanding the functions of chromosomal passenger proteins during mitosis.","PeriodicalId":344667,"journal":{"name":"2009 International Symposium on Micro-NanoMechatronics and Human Science","volume":"45 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2009-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131122456","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
C. Tercero, S. Ikeda, M. Matsushima, T. Fukuda, Erick Tijerino, M. Negoro, I. Takahashi
{"title":"Human blood pressure simulation for photoelastic stress analysis in models of vasculature","authors":"C. Tercero, S. Ikeda, M. Matsushima, T. Fukuda, Erick Tijerino, M. Negoro, I. Takahashi","doi":"10.1109/MHS.2009.5351809","DOIUrl":"https://doi.org/10.1109/MHS.2009.5351809","url":null,"abstract":"The development of a numerical criterion to evaluate the stress on models of vasculature has applications in evaluation of human skills, robots and medical tools. This criterion will enable better medical training for endovascular surgery and the development of better medical techniques and tools. We propose to use the stress produced by human blood pressure simulation in the wall of the model of vasculature as this criterion; and to measure the principal component of stress magnitude using photoelastic effect. For that we simulated human blood pressure with a 5.6% of average error, we developed a shielded urethane model of vasculature enabling water circulation and avoiding plastic deformation with pressures below 182 mmHg. We developed software to calculate the stress of the model wall. Stress produced by human blood pressure simulation and a guide wire were compared numerically in four ranges.","PeriodicalId":344667,"journal":{"name":"2009 International Symposium on Micro-NanoMechatronics and Human Science","volume":"25 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2009-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131381819","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Fabrication of self organized structure by controlling growth direction of plant cells","authors":"Kenta Yamamoto, K. Morishima","doi":"10.1109/MHS.2009.5351987","DOIUrl":"https://doi.org/10.1109/MHS.2009.5351987","url":null,"abstract":"In this paper, new fabrication methods using plant cell was proposed. First, to prevent disappearing of culture medium, we fabricated syringe injection type culture mold. Using this mold, we will fabricate any figure structure. Then, to improve fabrication process effectively, the mold which cultures plant cells was optimized. First, we investigated the relationship between growth speed and membrane thickness of the mold. And it was figured out that PDMS mold was the most suitable material to culture plant cells. We also figured out Young's modules of plant cell structure.","PeriodicalId":344667,"journal":{"name":"2009 International Symposium on Micro-NanoMechatronics and Human Science","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2009-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122445292","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Tsubasa Kitajima, A. Hirata, Chikako Iwashita, S. Yokobori, H. Hori
{"title":"Enzymatic and crystallographic characterization of archaeal tRNA splicing endonuclease","authors":"Tsubasa Kitajima, A. Hirata, Chikako Iwashita, S. Yokobori, H. Hori","doi":"10.1109/MHS.2009.5352027","DOIUrl":"https://doi.org/10.1109/MHS.2009.5352027","url":null,"abstract":"RNA splicing endonuclease, which is conserved in Eukarya and Archaea, removes introns from eukaryotic nuclear tRNA and archaeal all RNA species. There are three types of subunit composition in archaeal RNA splicing endonucleases, namely α2 (homodimer in some Euryarchaea), α4 (homotetramer in other Euryarchaea) and (αβ)2 (heterotetramer in the Crenarchaea) types. In Crenarchaea, introns in precursor tRNA (pre-tRNA) molecules are found not only in anticodon arm but also in several other regions in tRNA such as D- and T-loops, variable region, and aminoacyl stem. These introns have a consensus bulge-helix-bulge motif. Crenarchaeal RNA splicing endonuclease can remove introns in such non-canonical sites. However, the broad cleavage site-specificity of the enzyme remains exclusive. Here, we report the cleavage activity of recombinant RNA splicing endonuclease from hyperthermophilic crenarcheon Aeropyrum pernix (APE) by using the pre-tRNAThr (UGU) and pre-tRNAThr (CGU), in which introns are located in anticodon and D-loops, respectively. Furthermore we report that APE RNA splicing enzyme crystallizes in space group P3(1) with two or three heterotetramers in an asymmetric unit. An X-ray diffraction data set has been collected to 2.8 Å resolution. Structural determination is now underway.","PeriodicalId":344667,"journal":{"name":"2009 International Symposium on Micro-NanoMechatronics and Human Science","volume":"46 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2009-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126580775","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
J. Teramoto, Y. Yamanishi, El-Shimy H. Magdy, A. Hasegawa, M. Nakajima, T. Shimada, F. Arai, T. Fukuda, A. Ishihama
{"title":"Single live-bacterial cell assay of promoter activity and regulation: Escherichia coli gcl promoter","authors":"J. Teramoto, Y. Yamanishi, El-Shimy H. Magdy, A. Hasegawa, M. Nakajima, T. Shimada, F. Arai, T. Fukuda, A. Ishihama","doi":"10.1109/MHS.2009.5351949","DOIUrl":"https://doi.org/10.1109/MHS.2009.5351949","url":null,"abstract":"For measurement of the promoter activity and regulation within single bacterial cells, we constructed various types of the cell chip. After gelation of portions of E. coli culture wihin the cell chip, we have succeeded, for the first time, the real-time single-bacterial cell assay of promoter activity. Here we applied this newly developed system for measurement of the activity and regulation of E. coli gcl promoter.","PeriodicalId":344667,"journal":{"name":"2009 International Symposium on Micro-NanoMechatronics and Human Science","volume":"42 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2009-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132601093","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Minori Tokuda, A. Kiyohara, Takahisa Taguch, S. Kudoh
{"title":"The effects of the current stimulation on electrical activity in dissociated neurons","authors":"Minori Tokuda, A. Kiyohara, Takahisa Taguch, S. Kudoh","doi":"10.1109/MHS.2009.5352068","DOIUrl":"https://doi.org/10.1109/MHS.2009.5352068","url":null,"abstract":"Rat hippocampal neurons were cultured on a dish with 64 micro planer electrodes. We found that the silent and reproducible period lasting for 1 sec immediately after the activity evoked in advance. In addition, the repetitive stimuli suppress the spontaneously occurring bursting activity in frequency. These results suggest that distinct internal state of the neuronal circuit was triggered by an electrical stimulation, and these dynamics of network activity may contribute to information processing. In previous study, we developed the neuro-robot system in which the neurons were connected to a robot body and interacted with external world. By utilizing the dynamics of a living neuronal network, it can be designed that, so to speak, the robot, which behaves according to the history of the sensor inputs which the neuronal network has memorized. We are verifying whether the dynamics intrinsic in a neural network can contribute to the action determination of a creature, by moving such a model system.","PeriodicalId":344667,"journal":{"name":"2009 International Symposium on Micro-NanoMechatronics and Human Science","volume":"58 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2009-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128467901","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}