启动子活性和调控的单活细菌细胞测定:大肠杆菌gcl启动子

2009 International Symposium on Micro-NanoMechatronics and Human Science Pub Date : 2009-12-11 DOI:10.1109/MHS.2009.5351949

J. Teramoto, Y. Yamanishi, El-Shimy H. Magdy, A. Hasegawa, M. Nakajima, T. Shimada, F. Arai, T. Fukuda, A. Ishihama

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引用次数: 0

摘要

为了测量单个细菌细胞内启动子的活性和调控，我们构建了不同类型的细胞芯片。在细胞芯片内的大肠杆菌培养部分凝胶化后，我们第一次成功地进行了启动子活性的实时单细胞检测。本研究将该系统应用于大肠杆菌gcl启动子活性和调控的测定。

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Single live-bacterial cell assay of promoter activity and regulation: Escherichia coli gcl promoter

For measurement of the promoter activity and regulation within single bacterial cells, we constructed various types of the cell chip. After gelation of portions of E. coli culture wihin the cell chip, we have succeeded, for the first time, the real-time single-bacterial cell assay of promoter activity. Here we applied this newly developed system for measurement of the activity and regulation of E. coli gcl promoter.

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2009 International Symposium on Micro-NanoMechatronics and Human Science

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