2009 International Symposium on Micro-NanoMechatronics and Human Science最新文献_第2页

Fluorescent observation of individual DNA molecules on phospholipid bilayers and its application to analysis of DNA-protein interactions 磷脂双层上单个DNA分子的荧光观察及其在DNA-蛋白质相互作用分析中的应用

2009 International Symposium on Micro-NanoMechatronics and Human Science Pub Date : 2009-12-11 DOI: 10.1109/MHS.2009.5351907

H. Kurita, T. Takata, H. Yasuda, K. Takashima, A. Mizuno

{"title":"Fluorescent observation of individual DNA molecules on phospholipid bilayers and its application to analysis of DNA-protein interactions","authors":"H. Kurita, T. Takata, H. Yasuda, K. Takashima, A. Mizuno","doi":"10.1109/MHS.2009.5351907","DOIUrl":"https://doi.org/10.1109/MHS.2009.5351907","url":null,"abstract":"The advantage of single-molecule experiments on analysis of DNA-protein interactions is that it can manipulate the physical form of individual DNA molecules. In these experiments, surface treatments for anchoring individual DNA molecules are important. In this study, we carried out fluorescent observations of individual DNA molecules on phospholipid bilayers. First, we constructed a PDMS microchannel and neutravidin was immobilized on the glass surface to tether individual DNA molecules. And then, DOPC liposomes were applied to the microchannel and the lipid bilayers were constructed. Biotinylated DNA molecules labeled with fluorescent dyes were then injected to the channel and they were tethered to the glass surface. As a result, we obtained clear images of the DNA molecules. Intriguingly, in addition, we found that if the fluorescent observation was carried out with the phospholipid bilayers, photobleaching of the fluorescently-labeled DNA was slower by comparison with that without the bilayers. Moreover, we applied this method to DNA-protein interactions at a single-molecule level.","PeriodicalId":344667,"journal":{"name":"2009 International Symposium on Micro-NanoMechatronics and Human Science","volume":"89 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2009-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121365940","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mesoscale-object handling by temperature modulation of surface stresses 通过表面应力的温度调制处理中尺度物体

2009 International Symposium on Micro-NanoMechatronics and Human Science Pub Date : 2009-12-11 DOI: 10.1109/MHS.2009.5351741

E. Vela, M. Hafez, S. Régnier, S. Bouchigny

引用次数: 0

Risk management system based on uncertainty estimation by multi-agent 基于多智能体不确定性估计的风险管理系统

2009 International Symposium on Micro-NanoMechatronics and Human Science Pub Date : 2009-12-11 DOI: 10.20965/jrm.2010.p0456

Daichi Kato, K. Sekiyama, T. Fukuda

引用次数: 1

Fuzzy PID control of a wearable rehabilitation robotic hand driven by pneumatic muscles 气动肌肉驱动可穿戴康复机械手的模糊PID控制

2009 International Symposium on Micro-NanoMechatronics and Human Science Pub Date : 2009-12-11 DOI: 10.1109/MHS.2009.5352012

Jun Wu, Jian Huang, Yongji Wang, Kexin Xing, Qi Xu

{"title":"Fuzzy PID control of a wearable rehabilitation robotic hand driven by pneumatic muscles","authors":"Jun Wu, Jian Huang, Yongji Wang, Kexin Xing, Qi Xu","doi":"10.1109/MHS.2009.5352012","DOIUrl":"https://doi.org/10.1109/MHS.2009.5352012","url":null,"abstract":"Intensive task-oriented repetitive physical therapies need be provided by individualized interaction between the patients and the rehabilitation specialists to improve hand motor performance for those survived from stoke and traumatic brain injury. The goal of this research is to develop a novel wearable device for robotic assisted hand repetitive therapy. We designed a pneumatic muscle (PM) driven therapeutic device that is wearable and provides assistive forces required for grasping and finger extension. The robot has two distinct degrees of freedom at the thumb and all other fingers. The embedded sensors can feedback position and force information for robot control and quantitative evaluation of task performance. It is potential of providing supplemental at-home therapy in addition to in the clinic treatment. To realize the trajectory tracking control, a fuzzy PID controller is designed for the proposed device. The experimental results show angle tracking control of the robotic hand using the fuzzy PID controller has better performance than that using a conventional PID controller.","PeriodicalId":344667,"journal":{"name":"2009 International Symposium on Micro-NanoMechatronics and Human Science","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2009-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130048454","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 35

Fault tolerance analysis of MEMS holographic memory for DORGAs 用于DORGAs的MEMS全息存储器容错分析

2009 International Symposium on Micro-NanoMechatronics and Human Science Pub Date : 2009-12-11 DOI: 10.1109/MHS.2009.5352102

D. Seto, Minoru Watanabe

引用次数: 0

Micro-optical medical monitoring 微光医学监测

2009 International Symposium on Micro-NanoMechatronics and Human Science Pub Date : 2009-12-11 DOI: 10.1109/MHS.2009.5352064

H. Zappe

引用次数: 0

Preparation of cytochromes b5 with an extended COOH-terminal hydrophilic segment: Interaction of modified tail-anchored proteins with liposomes in different cholesterol content 带扩展cooh末端亲水段的细胞色素b5的制备:不同胆固醇含量的修饰尾锚定蛋白与脂质体的相互作用

2009 International Symposium on Micro-NanoMechatronics and Human Science Pub Date : 2009-12-11 DOI: 10.1109/MHS.2009.5352030

Y. Sakamoto, F. Takeuchi, M. Miura, Sam-Yong Park, M. Tsubaki

引用次数: 0

Functional shRNA expression system with reduced off-target effects 减少脱靶效应的功能性shRNA表达系统

2009 International Symposium on Micro-NanoMechatronics and Human Science Pub Date : 2009-12-11 DOI: 10.1109/MHS.2009.5351912

Y. Naito, K. Nishi, Aya Juni, K. Ui-Tei

{"title":"Functional shRNA expression system with reduced off-target effects","authors":"Y. Naito, K. Nishi, Aya Juni, K. Ui-Tei","doi":"10.1109/MHS.2009.5351912","DOIUrl":"https://doi.org/10.1109/MHS.2009.5351912","url":null,"abstract":"The capability to induce off-target effects by small interfering RNA (siRNA) is highly correlated with the calculated melting temperature (Tm) of the protein-free seed-target duplex, indicating that its thermodynamic stability is a major factor in determining the degree of the off-target effect. In this study, we analyzed changes in microarray expression profiles following transfection of short hairpin RNAs (shRNAs). In a similar manner to siRNA, no or little off-target effects were observed by shRNA with a low Tm value in the seed-target duplex, whereas a high degree of off-target effect was induced by shRNA with a high Tm. Furthermore, shRNAs were often cleaved by Drosha and/or Dicer at the shifted sites in their primary transcripts, resulting in unexpected siRNA products with shifted sequences. Based on these results, the following conditions should be satisfied to ensure a functional shRNA with reduced off-target effects: the presence of A/U residues at nucleo tide positions 1 and −1 from the 5' end of the guide strand, three to six A/Us in nucleotide positions 2–7, G/C at positions 19 and 18, and no GC stretch of more than nine nucleotides. In addition, the Tm values of the seed regions in both strands should be low.","PeriodicalId":344667,"journal":{"name":"2009 International Symposium on Micro-NanoMechatronics and Human Science","volume":"43 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2009-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114319928","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Design of bio-manipulation with micro water jet 微水射流生物操纵的设计

2009 International Symposium on Micro-NanoMechatronics and Human Science Pub Date : 2009-12-11 DOI: 10.1109/MHS.2009.5351991

Yalikun Yaxar, T. Hoshino, K. Morishima

引用次数: 0

User friendly two-fingered cell manipulation system 用户友好的两指细胞操作系统

2009 International Symposium on Micro-NanoMechatronics and Human Science Pub Date : 2009-12-11 DOI: 10.1109/MHS.2009.5351935

Daiki Kawakami, K. Ohara, Y. Mae, T. Takubo, T. Tanikawa, T. Arai

引用次数: 0