Rossiiskii parazitologicheskii zhurnal最新文献

{"title":"Study of the tolerance of high doses of drugs for external use based on fipronil, praziquantel, moxidectin and pyriproxyfen","authors":"N. V. Makhvatova, E. O. Kachanova","doi":"10.31016/1998-8435-2023-17-1-114-123","DOIUrl":"https://doi.org/10.31016/1998-8435-2023-17-1-114-123","url":null,"abstract":"The purpose of the research is to study the tolerance of increased doses of topical preparations based on fipronil, praziquantel, moxidectin and pyriproxyfen in dogs and cats at increased therapeutic doses.Materials and methods. In the laboratory of ectoparasitoses on the basis of VNIIP, a branch of the Federal State Budget Scientific Institution of the Federal Scientific Center of VIEV RAS, two preparations were developed in the form of a solution for external use for dogs and cats based on praziquantel, pyriproxyfen, imidacloprid and moxidectin. Work on the study of tolerance to high doses was carried out on 15 kittens and 15 puppies under the age of one year, 15 adult cats and 15 adult dogs. Animals were divided into 8 experimental and 4 control groups according to the principle of analogues, 5 animals each. The drugs were used at 1.5 and 2 times the increased therapeutic dose four times with an interval of 7 days. The animals were observed for 30 days; noted the general condition, behavior, appetite, controlled weight and body temperature. Before the start of the experiment and 30 days after the start of the use of the drugs, blood was taken for general clinical and biochemical studies.Results and discussion. It was found that drugs in 1.5 and 2 times increased therapeutic doses, applied four times with an interval of 7 days, did not have a negative effect on carnivores. There were no changes in behavior, body temperature and weight, physical condition, clinical status, hematological and biochemical parameters of blood and functions of internal organs. The drug was well tolerated by dogs and cats of different breeds of different age groups, as well as by puppies and kittens. Manifestations of any allergic reactions were not observed. The obtained results of the studies confirm the safety of the use of drugs in practice in the recommended dosing regimen.","PeriodicalId":34353,"journal":{"name":"Rossiiskii parazitologicheskii zhurnal","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44274657","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of intestinal coccidiosis on weight gain of young cattle","authors":"A. Reshetnikova, E. Klimova","doi":"10.31016/1998-8435-2023-17-1-99-104","DOIUrl":"https://doi.org/10.31016/1998-8435-2023-17-1-99-104","url":null,"abstract":"The purpose of the research is to study the effect of coccidiosis infection (cryptosporidiosis, eimeriosis) on the average daily gains in live weight of young cattle.Materials and methods. The studies were carried out on animals spontaneously infected with Eimeria spp. And Cryptosporidium spp. According to the principle of analogues, 4 groups of animals were formed, 10 heads each. In the first experiment, the average daily weight gain of 1-20-day-old animals infected with Cryptosporidium (1st experimental group) and clinically healthy calves (1st control group) was compared. For the second experiment, calves at the age of 2-4 months, infected with Eimeria spp. (2nd), were selected, young animals free from infection were selected in the second control group.Results and discussion. In calves infected with Cryptosporidium spp., the average daily gains ranged from 0.597±0.017 to 0.675±0.018 kg. The decrease in growth per day relative to the animals of the control group was, on average, 0.346 kg. The maximum loss of weight gain, 11.0±0.88 kg, was recorded in November. A similar dynamic of the decrease in average daily weight gain was established in animals at eimeriosis. During the research period, the shortfall in live weight of calves infected with Eimeria spp. was 21.5±2.6 kg, which is 12.3 kg less relative to the mass of calves infected with Cryptosporidium spp. Sharp fluctuations in the reduction of average daily weight gain in this group were not recorded: on average, 0.248±0.113 kg relative to the control group. Thus, the reason for the lack of live weight gain (up to 40 %) in calves in the farm of the Uvinsky district of the Udmurt Republic is intestinal coccidiosis. The minimum indicator of the average daily gain in live weight (0.597±0.017 kg) falls on the group of calves infected with cryptosporidiosis, which is almost 2 times less relative to the gain of animals from the control group.","PeriodicalId":34353,"journal":{"name":"Rossiiskii parazitologicheskii zhurnal","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49040478","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative diagnostic efficacy of microscopy, combined flotation and polymerase chain reaction to detect Giardia spp. in dogs and cats","authors":"","doi":"10.31016/1998-8435-2023-17-1-91-98","DOIUrl":"https://doi.org/10.31016/1998-8435-2023-17-1-91-98","url":null,"abstract":"The purpose of the research is to perform a comparative diagnostic efficacy of microscopy, combined flotation method and polymerase chain reaction (PCR) used to detect Giardia spp. in dogs and cats.Materials and methods. The efficacy was compared between three methods to detect Giardia spp. on 60 fecal samples from dogs and cats from the Zaitsev+ and Paster Veterinary Laboratory.Results and discussion. The highest diagnostic efficacy was established for PCR, 78.3%; the diagnostic efficacy of combined flotation was 68.3%; the lowest diagnostic efficacy was found for the microscopy method, 43.3%. The combined flotation method to detect Giardia spp. is widely used in laboratory practice as it allows detection of other intestinal parasites in addition to Giardia spp. Microscopy is the fastest and simplest method for detecting not only Giardia spp. but also other intestinal parasites.","PeriodicalId":34353,"journal":{"name":"Rossiiskii parazitologicheskii zhurnal","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47025491","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of intensity of infection on morphological characteristics of Trichinella spiralis larvae at experimental infection of white rats and their distribution in muscles","authors":"O. Zhdanova, A. Uspensky, L. Napisanova, O. V. Chasovskikh, D. V. Rossokhin, O. Andreyanov, N. S. Malysheva, E. O. Kachanova","doi":"10.31016/1998-8435-2023-17-1-74-83","DOIUrl":"https://doi.org/10.31016/1998-8435-2023-17-1-74-83","url":null,"abstract":"The purpose of the research is to study the morphological changes in the capsules of Trichinella spiralis larvae and their distribution in muscles.Materials and methods. In the experiment, 12 white rats were used, divided into 3 groups of 4 animals each. Rats of the first group were infected with T. spiralis larvae at a dose of 5 larvae per 1 g of body weight, the second – at a dose of 40 larvae per 1 g, rats of the 3rd group served as control and were not infected. The selective dispersal of larvae was studied by determining the intensity of infection in post-mortem studies of the main muscle groups of the animal and measuring the capsules of larvae in different muscle groups.Results and discussion. In the entire muscle mass, 45±10 T. spiralis larvae/animal were found in the 1st group, in the 2nd group the number of larvae was 2250±180, in the control group no T. spiralis larvae were found. It has been established that the distribution of T. spiralis larvae in the muscles of infected animals depends on the dose of infection: at low doses, the largest number was found in the gastrocnemius muscles and diaphragm, at high doses, the number of larvae in the muscles of the head sharply increases.","PeriodicalId":34353,"journal":{"name":"Rossiiskii parazitologicheskii zhurnal","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42566121","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Special features of helminth fauna of Mustelidae in the Polistovsky National Nature Reserve and development factors","authors":"I. Tsvetkov, K. N. Tsvetkova, N. P. Korablev","doi":"10.31016/1998-8435-2023-17-1-43-56","DOIUrl":"https://doi.org/10.31016/1998-8435-2023-17-1-43-56","url":null,"abstract":"The purpose of the research is to study the species composition of helminths of the Mustelidae family taking into account biotopic and trophic factors in the Polistovsky National Nature Reserve.Materials and methods. Faeces of Mustela putorius, Neovison vison, Lutra lutra and Martes martes were studied to determine a helminth fauna composition in the Mustelidae family and the factors affecting its development. Helminth eggs were detected using coproovoscopy. For detection of nematode eggs in the feces, the Forate sugar solution flotation method was used; for detection of trematode and cestode eggs, the successive washing method was used. Sexually mature nematodes of the genus Eucoleus were obtained from the pine marten by the method of B. V. Romashov. Species diagnostics of helminth eggs was carried out according to determinants, monographic and other works. The morphometric study of eggs was carried out using the Screen Meter computer program with an accuracy of 0.001 mm. The final diagnostics was carried out by comparing eggs from the faeces of predators and eggs from mature helminths. To study the trophic factor in helminth fauna development, the faeces composition was studied.Results and discussion. The studied mustelids were infected by nematodes Eucoleus sp., Eucoleus aerophilus and Capillaria putorii, and trematodes Isthmiophora melis and Opisthorchiidae sp. Helminth eggs were found in 42.3% samples. The helminth eggs prevail in the material from the polecat and American mink (60.5%) over that from the otter (30.5%). Stenobionts, stenophagy and morphophysiology determine a low infection rate in the otter versus the mink and polecat. Mixed coniferous-small-leafed forest shows a richer list of helminths than the marsh. The obtained data evidences limited helminth circulation in high moor. It is not possible to differentiate eggs of E. aerophilus and E. trophimenkovi according to the previously proposed shape index due to the high individual variability of eggs. High moor as a specific biotope reduces biodiversity including helminths.","PeriodicalId":34353,"journal":{"name":"Rossiiskii parazitologicheskii zhurnal","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46004960","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of the taxonomic affiliation of Cryptosporidium spp. in piglets in the conditions of the north-west of the Russian Federation using molecular genetic methods","authors":"A. Kryazhev, A. Novikov","doi":"10.31016/1998-8435-2023-17-1-84-90","DOIUrl":"https://doi.org/10.31016/1998-8435-2023-17-1-84-90","url":null,"abstract":"The purpose of the research is to determine taxa of the genus Cryptosporidium species in pigs using molecular genetic methods in the north-west of the Russian Federation.Materials and methods. Fecal samples were taken from pigs of different age groups on farms of different types of incorporation that differ in climatic and geographical zones and animal keeping technologies in the Vologda Region. Microscopic research methods identified “positive” samples in which Cryptosporidium species were present; they were sorted out and deep-frozen. Then the samples were examined using the equipment of the resource center «Genomic Technologies, Proteomics and Cell Biology» of ARRIAM. The Cryptosporidium species in the fecal samples from farm animals were identified using high-throughput sequencing of 18S rRNA gene amplicon libraries obtained by a nested polymerase chain reaction (PCR) assay.Results and discussion. A primer system was designed for the nested PCR to amplify a potentially species-specific 393 bp fragment of the 18S rRNA gene. The sequence of the ILL_R2_ Zheng primer was modified with included degenerated positions to make the primer more versatile. As a result of sequencing of the libraries of 18S rRNA gene fragments obtained with the selected primers and subsequent taxonomic analysis of the nucleotide sequences, it was shown that all the studied samples included representatives of only one species, Cryptosporidium scrofarum.","PeriodicalId":34353,"journal":{"name":"Rossiiskii parazitologicheskii zhurnal","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43478393","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Trematode fauna of fish inhabiting reservoirs of the European part of Russia","authors":"N. N. Romanova, N. Golovina, A. Vishtorskaya, P. Golovin","doi":"10.31016/1998-8435-2023-17-1-28-42","DOIUrl":"https://doi.org/10.31016/1998-8435-2023-17-1-28-42","url":null,"abstract":"The purpose of the research is an ecological and faunal analysis of trematodes in fish inhabiting reservoirs of the European part of Russia.Materials and methods. Helminthological studies were conducted in 23 reservoirs of the European part of Russia from 2011 to 2021. The fish aged two to seven years were analyzed by methods generally accepted in ichthyoparasitology.Results and discussion. Twelve fish species from families Cyprinidae, Percidae, Esocidae and Odontobutidae were found to be infected with 29 trematode species from 14 genera which were represented by 68.9% of metacercariae. Most trematodes had a wide specificity: Tylodelphys clavata was found in 9 fish species; Diplostomum spathaceum and Paracoenogonimus ovatus in 7 species and Apophallus muehlingi in 6 species. The expanded host range for T. podicipina was observed. A. muehlingi, which is an alien species for the Volga-Caspian fisheries basin was identified in most reservoirs. Trematodes by their prevalence in reservoirs are divided into background (5 species), common (6), rare (8) and very rare (10) trematodes. Trematode fauna of fish in reservoirs included 6 to 16 species. The highest species diversity was detected in the Belgorod (16 species), Yakhroma (13 species), Uglich and Chelnav (12 species), Pestovsk and Pyalovsk (11 species) reservoirs. Fifteen trematode species were found in the perch; 12, in the bream and roach; 9, in the pike perch; 8, in the pike and rudd; 6, in the ruff and white bream; 4, in the Volga pikeperch and crucian carp; 3, in the tench; and 1, in the Amur sleeper. The formed foci of trematode infections were observed, namely, postodiplostomosis and ichthyocotylurosis infection. Three trematode species, Pseudoamphistomum truncatum, A. muehlingi and Rossicotrema donicum, were detected that pose a real and potential danger to human health and warm-blooded animals.","PeriodicalId":34353,"journal":{"name":"Rossiiskii parazitologicheskii zhurnal","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44268265","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Myxosporeans of the genus Kudoa (localization in the fish body, the form of spores and ways of their entry into the environment and into new hosts)","authors":"","doi":"10.31016/1998-8435-2023-17-1-57-73","DOIUrl":"https://doi.org/10.31016/1998-8435-2023-17-1-57-73","url":null,"abstract":"The purpose of the research is to study the localization of myxosporeans of the genus Kudoa Meglitsch, 1947 in body fish and possible ways for them to master organs and tissues in the process of evolution, the form of spores associated with localization, as well as the ways of their release into the environment and getting into new hosts.Materials and methods. The work is based on our own materials on fish myxosporeans from the Black, Azov and Mediterranean seas, as well as the Atlantic, Indian and Pacific oceans, collected in 1987–2021. In total, we have studied more than 12,000 sp. more than 100 species of fish. We also analyzed descriptions of 126 species of myxosporeans of the genus Kudoa, known in the world scientific literature until 2021 inclusive. For this, 450 domestic and foreign works have been worked out. The material was collected by the method of incomplete parasitological dissections. Smears made from tissues were processed according to the generally accepted method with the manufacture of permanent preparations. All measurements were made according to the standard method. To assess the number of parasites, standard indicators were used: the extensiveness and intensity of infection.Results and discussion. In total, we studied 19 species of myxosporeans of the genus Kudoa and 8 species of Kudoa spp. Six species (K. stellula, K. niluferi, K. anatolica from the Black Sea, K. unicapsula from the Mediterranean Sea, K. borimiri and K. igori from the South China (or East) Sea) were new to science. We have established 24 places of parasitism of myxosporeans of this genus in the body of fish. 83 species (66%, or 2/3 species) are found in muscles, sometimes affecting (8 species) other tissues and organs of the host. 43 species (34%, or 1/3 species) of representatives of the genus Kudoa are never found in muscle tissue. The primary sites of parasitism of myxosporeans of the genus Kudoa were probably the intestinal wall and gallbladder, then the parasites mastered other internal organs of fish and, ultimately, brain and muscle. The hypothetical ways of getting spores into the external environment and into new hosts are indicated. It is assumed that the most ancient forms are bivalve Kudoa, parasitizing in the gallbladder, four-valve Kudoa of an unusual shape, resembling representatives of other genera and parasitizing mainly in internal organs, as well as four-valve Kudoa with a classical square spore shape, rounded valve tops, with four equal polar capsules and the smallest sizes. Spores with a pointed stellate shape (only muscular forms) and spores with five or more valves and polar capsules (occurring in muscle and brain and having a larger size) should be considered to have arisen at a later time. The ways of getting spores into the environment and into new hosts are highlighted.","PeriodicalId":34353,"journal":{"name":"Rossiiskii parazitologicheskii zhurnal","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49210457","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Species composition and ecology of blood-sucking mosquitoes (Diptera, Culicidae) in Yakutia","authors":"","doi":"10.31016/1998-8435-2023-17-1-19-27","DOIUrl":"https://doi.org/10.31016/1998-8435-2023-17-1-19-27","url":null,"abstract":"The purpose of the research is to study the species composition and ecology of mosquitoes in Yakutia.Materials and methods. The research was performed for herd horses and cattle on pastures in Central, Western and Southern Yakutia in 2005–2021. Faunistic collections were made and the number of blood-sucking mosquito imago was recorded for animals using an entomological net with removable bags, while the average abundance rate of mosquitoes was calculated per 10 net movements in a figure-eight pattern around from 10 repetitions when studying the seasonal abundance dynamics and from 5 repetitions when studying the circadian activity rhythm.Results and discussion. In Yakutia, 15 species of blood-sucking mosquitoes of the family Culicidae were found that belong to three genera: Anopheles, Culiseta, and Aedes. Biotopes occupied by mosquito larvae of the genus Aedes include 74–126 specimens/m2. The first mosquitoes were recorded near an animal bait in the first decade of May. The mass mosquito flight period was observed from the third decade of June to the middle of the second decade of July. The total mosquitofl ight period for the season was 121–124 days. The flight of blood-sucking mosquitoes on the forest pasture continues around the clock, from the third decade of June to the middle of the second decade of July.","PeriodicalId":34353,"journal":{"name":"Rossiiskii parazitologicheskii zhurnal","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47455381","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biodiversity of helminths in Karakul sheep in the Khorezm Oasis of Uzbekistan","authors":"A. Akbarov, F. Akramova, U. A. Shakarbayev, D. Azimov","doi":"10.31016/1998-8435-2023-17-1-11-18","DOIUrl":"https://doi.org/10.31016/1998-8435-2023-17-1-11-18","url":null,"abstract":"The purpose of the research is to study the helminth fauna and the structure of helminthocenoses of Karakul sheep in the Khorezm Oasis of Uzbekistan.Materials and methods. The material for this study was helminths collected by conventional methods from Karakul sheep from farms of different types in the Khorezm Region. A total of 13 sheep were studied. To identify the dominant helminth infections in sheep, 110 sets of individual organs were examined by the method of partial helminthological dissections. Helminths were collected in slaughterhouses of the Bagat, Kushkupyr, Urgench, Yangiaryk and Shavat Districts of the Khorezm Region. Additionally, the helminths collected from sheep in the Khorezm Region for 2015–2021 were used.Results and discussion. We found that the helminth fauna of Karakul sheep from the Khorezm Oasis was represented by 22 species of the classes Cestoda, Trematoda and Nematoda. Cestodes were represented by 6 species, trematodes by 3 species, and nematodes by 13 species. The faunistic composition of helminths in studied animals was noticeably depleted as compared to other Uzbekistan regions due to natural and ecological conditions of the Khorezm Oasis. The total helminth infection rate in sheep was 100%. The intensity of infection varied depending on the season of the year and the age of sheep and ranged from tens to several thousand specimens. Almost all organs and systems of the animals were found to be ecological niches of the above helminths. Most of helminth species were common to both domesticated and wild ungulates.","PeriodicalId":34353,"journal":{"name":"Rossiiskii parazitologicheskii zhurnal","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44065253","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}