Biocell : official journal of the Sociedades Latinoamericanas de Microscopia Electronica ... et. al最新文献

Anti-proliferative effect of Annona extracts on breast cancer cells. 番荔枝提取物对乳腺癌细胞的抗增殖作用。

Biocell : official journal of the Sociedades Latinoamericanas de Microscopia Electronica ... et. al Pub Date : 2023-08-28 DOI: 10.32604/biocell.2023.029076

M. Veisaga, M. Ahumada, Stacy Soriano, L. Acuña, W. Zhang, Ivy Leung, Robert P. Barnum, Manuel A. BARBIERI

{"title":"Anti-proliferative effect of Annona extracts on breast cancer cells.","authors":"M. Veisaga, M. Ahumada, Stacy Soriano, L. Acuña, W. Zhang, Ivy Leung, Robert P. Barnum, Manuel A. BARBIERI","doi":"10.32604/biocell.2023.029076","DOIUrl":"https://doi.org/10.32604/biocell.2023.029076","url":null,"abstract":"Backgorund\u0000Fruits and seed extracts of Annona montana have significant cytotoxic potential in several cancer cells. This study evaluates the effect of A. montana leaves hexane extract on several signaling cascades and gene expression in metastatic breast cancer cells upon insulin-like growth factor-1 (IGF-1) stimulation.\u0000\u0000\u0000Methods\u0000MTT assay was performed to determine the proliferation of cancer cells. Propidium iodide staining and flow cytometry analysis of Annexin V binding was utilized to measure the progression of the cell cycle and the induction of apoptosis. Protein expression and phosphorylation were determined by western blotting analysis to examine the underlying cellular mechanism triggered upon treatment with A. montana leaves hexane extract.\u0000\u0000\u0000Results\u0000A. montana leaves hexane (sub-fraction V) blocked the constitutive stimulation of the PI3K/mTOR signaling pathways. This inhibitory effect was associated with apoptosis induction as evidenced by the positivity with Annexin V and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNNEL) staining, activation of caspase-3, and cleavage of PPAR. It also limited the expression of various downstream genes that regulate proliferation, survival, metastasis, and angiogenesis (i.e., cyclin D1, survivin, COX-2, and VEGF). It increased the expression of p53 and p21. Interestingly, we also observed that this extract blocked the activation of AKT and ERK without affecting the phosphorylation of the IGF-1 receptor and activation of Ras upon IGF-1 stimulation.\u0000\u0000\u0000Conclusion\u0000Our study indicates that A. montana leaves (sub-fraction V) extract exhibits a selective anti-proliferative and proapoptotic effect on the metastatic MDA-MB-231 breast cancer cells through the involvement of PI3K/AKT/mTOR/S6K1 pathways.","PeriodicalId":342778,"journal":{"name":"Biocell : official journal of the Sociedades Latinoamericanas de Microscopia Electronica ... et. al","volume":"32 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131412463","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recent biomedical advances enabled by HaloTag technology HaloTag技术使生物医学取得了最新进展

Biocell : official journal of the Sociedades Latinoamericanas de Microscopia Electronica ... et. al Pub Date : 2022-04-22 DOI: 10.32604/biocell.2022.018197

Weiyu Chen, Muhsin H Younis, Zhongkuo Zhao, W. Cai

{"title":"Recent biomedical advances enabled by HaloTag technology","authors":"Weiyu Chen, Muhsin H Younis, Zhongkuo Zhao, W. Cai","doi":"10.32604/biocell.2022.018197","DOIUrl":"https://doi.org/10.32604/biocell.2022.018197","url":null,"abstract":"The knowledge of interactions among functional proteins helps researchers understand disease mechanisms and design potential strategies for treatment. As a general approach, the fluorescent and affinity tags were employed for exploring this field by labeling the Protein of Interest (POI). However, the autofluorescence and weak binding strength significantly reduce the accuracy and specificity of these tags. Conversely, HaloTag, a novel self-labeling enzyme (SLE) tag, could quickly form a covalent bond with its ligand, enabling fast and specific labeling of POI. These desirable features greatly increase the accuracy and specificity, making the HaloTag a valuable system for various applications ranging from imaging to immobilization of POI. Notably, the HaloTag technique has already been successfully employed in a series of studies with excellent efficiency. In this review, we summarize the development of HaloTag and recent advanced investigations associated with HaloTag, including in vitro imaging (e.g., POI imaging, cellular condition monitoring, microorganism imaging, system development), in vivo imaging, biomolecule immobilization (e.g., POI collection, protein/nuclear acid interaction and protein structure analysis), targeted degradation (e.g., L-AdPROM), and more. We also present a systematic discussion regarding the future direction and challenges of the HaloTag technique.","PeriodicalId":342778,"journal":{"name":"Biocell : official journal of the Sociedades Latinoamericanas de Microscopia Electronica ... et. al","volume":"48 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2022-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"120958608","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Synergy of single-cell sequencing analyses and in vivo lineage-tracing approaches: A new opportunity for stem cell biology 单细胞测序分析和体内谱系追踪方法的协同作用:干细胞生物学的新机遇

Biocell : official journal of the Sociedades Latinoamericanas de Microscopia Electronica ... et. al Pub Date : 2022-03-11 DOI: 10.32604/biocell.2022.018960

Yuki Matsushita, W. Ono, N. Ono

引用次数: 2

New paradigms in regenerative engineering: Emerging role of extracellular vesicles paired with instructive biomaterials 再生工程的新范例:细胞外囊泡与指导性生物材料配对的新作用

Biocell : official journal of the Sociedades Latinoamericanas de Microscopia Electronica ... et. al Pub Date : 2022-02-07 DOI: 10.32604/biocell.2022.018781

W. Swanson, Yuji Mishina

引用次数: 4

Stem cells in intervertebral disc regeneration–more talk than action? 干细胞在椎间盘再生中的应用——空谈少行动?

Biocell : official journal of the Sociedades Latinoamericanas de Microscopia Electronica ... et. al Pub Date : 2021-12-15 DOI: 10.32604/biocell.2022.018432

P. Kraus, Ankit Samanta, Sina Lufkin, T. Lufkin

引用次数: 3

Proteogenomics for pediatric brain cancer 儿童脑癌的蛋白质基因组学研究

Biocell : official journal of the Sociedades Latinoamericanas de Microscopia Electronica ... et. al Pub Date : 2021-09-01 DOI: 10.32604/biocell.2021.017369

M. Simonian

引用次数: 0

Aneuploidy: An Opportunity Within Single-Cell RNA Sequencing Analysis 非整倍体:单细胞RNA测序分析的机会

Biocell : official journal of the Sociedades Latinoamericanas de Microscopia Electronica ... et. al Pub Date : 2021-06-26 DOI: 10.32604/biocell.2021.017296

J. Delaney

{"title":"Aneuploidy: An Opportunity Within Single-Cell RNA Sequencing Analysis","authors":"J. Delaney","doi":"10.32604/biocell.2021.017296","DOIUrl":"https://doi.org/10.32604/biocell.2021.017296","url":null,"abstract":"Single-cell sequencing data has transformed the understanding of biological heterogeneity. While many flavors of single-cell sequencing have been developed, single-cell RNA sequencing (scRNA-seq) is currently the most prolific form in published literature. Bioinformatic analysis of differential biology within the population of cells studied relies on inferences and grouping of cells due to the spotty nature of data within individual cell scRNA-seq gene counts. One biologically relevant variable is readily inferred from scRNA-seq gene count tables regardless of individual gene representation within single cells: aneuploidy. Since hundreds of genes are present on chromosome arms, high-quality inferences of aneuploidy can be made from scRNA-seq datasets. This viewpoint summarizes how utilization of these bioinformatic pipelines can benefit scRNA-seq studies, particularly in oncology wherein aneuploidy is both rampant and a hallmark of the studied disease. Awareness and use of these analytical pipelines will improve each field’s ability to understand the studied diseases. Authors are encouraged to attempt these aneuploid analyses when reporting scRNA-seq data, much like copy-number variants are commonly reported in bulk genome sequencing data.","PeriodicalId":342778,"journal":{"name":"Biocell : official journal of the Sociedades Latinoamericanas de Microscopia Electronica ... et. al","volume":"40 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2021-06-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121672343","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Post-treatment with plant extracts used in Brazilian folk medicine caused a partial reversal of the antiproliferative effect of glyphosate in the Allium cepa test. 用巴西民间医学中使用的植物提取物进行后处理，导致葱试验中草甘膦的抗增殖作用部分逆转。

Biocell : official journal of the Sociedades Latinoamericanas de Microscopia Electronica ... et. al Pub Date : 2013-08-01 DOI: 10.32604/BIOCELL.2013.37.023

V. Frescura, A. W. Kuhn, H. D. Laughinghouse, J. T. Paranhos, S. Tedesco

{"title":"Post-treatment with plant extracts used in Brazilian folk medicine caused a partial reversal of the antiproliferative effect of glyphosate in the Allium cepa test.","authors":"V. Frescura, A. W. Kuhn, H. D. Laughinghouse, J. T. Paranhos, S. Tedesco","doi":"10.32604/BIOCELL.2013.37.023","DOIUrl":"https://doi.org/10.32604/BIOCELL.2013.37.023","url":null,"abstract":"Species of the genus Psychotria are used for multiple purposes in Brazilian folk medicine, either as water infusions, baths or poultices. This study was aimed to evaluate the genotoxic and antiproliferative effects of infusions of Psychotria brachypoda and P. birotula on the Allium cepa test. Exposure to distilled water was used as a negative control, while exposure to glyphosate was used as a positive control. The interaction of extracts (as a post-treatment) with the effects of glyphosate was also studied. Results showed that glyphosate and the extracts of both P. brachypoda and P. birotula reduced the mitotic index as compared with the negative control (distilled water). Surprisingly, however, both extracts from P. brachypoda and P. birotula caused a partial reversal of the antiproliferative effect of glyphosate when used as a post-treatment. Glyphosate also induced the highest number of cells with chromosomal alterations, which was followed by that of P. birotula extracts. However, the extracts from P. brachypoda did not show any significant genotoxic effect. Post-treatment of glyphosate-treated samples with distilled water allowed a partial recovery of the genotoxic effect of glyphosate, and some of the Psychotria extracts also did so. Notably, post-treatment of glyphosate-treated samples with P. brachypoda extracts induced a statistically significant apoptotic effect. It is concluded that P. brachypoda extracts show antiproliferative effects and are not genotoxic, while extracts of P. birotula show a less potent antiproliferative effect and may induce chromosomal abnormalities. The finding of a partial reversion of the effects of glyphosate by a post-treatment with extracts from both plants should be followed up.","PeriodicalId":342778,"journal":{"name":"Biocell : official journal of the Sociedades Latinoamericanas de Microscopia Electronica ... et. al","volume":"33 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2013-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125159933","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 14

Confocal laser scanning microscopy and immunohistochemistry of cerebellar Lugaro cells. 小脑Lugaro细胞的共聚焦激光扫描显微镜和免疫组织化学。

Biocell : official journal of the Sociedades Latinoamericanas de Microscopia Electronica ... et. al Pub Date : 2013-08-01 DOI: 10.32604/BIOCELL.2013.37.029

O. Castejón

{"title":"Confocal laser scanning microscopy and immunohistochemistry of cerebellar Lugaro cells.","authors":"O. Castejón","doi":"10.32604/BIOCELL.2013.37.029","DOIUrl":"https://doi.org/10.32604/BIOCELL.2013.37.029","url":null,"abstract":"The present paper shows by means of confocal laser scanning microscopy the immunoreactivity of rat cerebellar Lugaro cells for calbindin, synapsin-I, PSD-95, GluR1, CaMKII alpha, and N-cadherin. Lugaro cells were easily characterized by their location beneath Purkinje cells. Calbindin revealed immunoreactivity in the cell body, and the axonal and dendritic processes. Synapsin-I labelled the presynaptic endings on Lugaro cells. Synapsin-I and PSD-95 immunoreactivity demonstrated the localization of presynaptic and postsynaptic endings surrounding cell soma, corresponding to afferent extrinsic and intrinsic cerebellar fibers. GluR1 immunoreactivity of the soma and cell processes indicates that Lugaro cells have functional ionotropic glutamate receptors that regulate calcium levels. CaMKII alpha immunoreactivity of Lugaro cell soma and processes suggest its participation as a molecular switch for long-term information storage, and serving as a molecular basis of long-term synaptic memory. N-cadherin immunoreactivity was correlated with somato-somatic and somato-dendritic junctions between Lugaro cells and their synaptic connections.","PeriodicalId":342778,"journal":{"name":"Biocell : official journal of the Sociedades Latinoamericanas de Microscopia Electronica ... et. al","volume":"2013 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2013-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133852183","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Creatine supplementation in trained rats causes changes in myenteric neurons and intestinal wall morphometry. 在训练大鼠中补充肌酸可引起肌神经细胞和肠壁形态的改变。

Biocell : official journal of the Sociedades Latinoamericanas de Microscopia Electronica ... et. al Pub Date : 2013-08-01 DOI: 10.32604/BIOCELL.2013.37.037

S. M. D. de Moraes, Thais Andréia Brogio, J. Zanoni, M. Zapater, S. B. Peres, L. Hernandes

{"title":"Creatine supplementation in trained rats causes changes in myenteric neurons and intestinal wall morphometry.","authors":"S. M. D. de Moraes, Thais Andréia Brogio, J. Zanoni, M. Zapater, S. B. Peres, L. Hernandes","doi":"10.32604/BIOCELL.2013.37.037","DOIUrl":"https://doi.org/10.32604/BIOCELL.2013.37.037","url":null,"abstract":"Creatine is widely used by athletes as an ergogenic resource. The aim of this study was to evaluate the influence of creatine supplementation on the duodenum of rats submitted to physical training. The number and myenteric neuronal cell bodies as well mucosal and muscular tunic morphometry were evaluated. Control animals received a standard chow for 8 weeks, and the treated ones received the standard chow for 4 weeks and were later fed with the same chow but added with 2% creatine. Animals were divided in groups: sedentary, sedentary supplemented with creatine, trained and trained supplemented with creatine. The training consisted in treadmill running for 8 weeks. Duodenal samples were either processed for whole mount preparations or for paraffin embedding and hematoxylin-eosin staining for histological and morphometric studies of the mucosa, the muscular tunic and myenteric neurons. It was observed that neither creatine nor physical training alone promoted alterations in muscular tunic thickness, villus height or crypts depth, however, a reduction in these parameters was observed when both were associated. The number of myenteric neurons was unchanged, but the neuronal cell body area was reduced in trained animals but not when training and creatine was associated, suggesting a neuroprotector role of this substance.","PeriodicalId":342778,"journal":{"name":"Biocell : official journal of the Sociedades Latinoamericanas de Microscopia Electronica ... et. al","volume":"20 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2013-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125129524","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3