Biocell : official journal of the Sociedades Latinoamericanas de Microscopia Electronica ... et. al最新文献_第10页

Morphology and structure of the pollen cone and pollen grain of the Araucaria species from Argentina. 阿根廷阿瓦卡利亚种花粉球果和花粉粒的形态和结构。

Biocell : official journal of the Sociedades Latinoamericanas de Microscopia Electronica ... et. al Pub Date : 2008-04-01 DOI: 10.32604/BIOCELL.2008.32.049

Georgina M. Del Fueyo, M. Caccavari, Elizabeth Dome

{"title":"Morphology and structure of the pollen cone and pollen grain of the Araucaria species from Argentina.","authors":"Georgina M. Del Fueyo, M. Caccavari, Elizabeth Dome","doi":"10.32604/BIOCELL.2008.32.049","DOIUrl":"https://doi.org/10.32604/BIOCELL.2008.32.049","url":null,"abstract":"The pollen cone and the pollen grain of the two Argentinean species of Araucaria are described with LM, SEM and TEM. Primordia of pollen cones are formed in April and May and reach maturity by mid-October in A. angustifolia (Bert.) O. Kuntze and by mid-November in A. araucana. (Mol.) K. Koch. Characters of the mature pollen cones and microsporophylls between both taxa are clearly differentiated. Pollen grains are spheroidal-subspheroidal, inaperturate, and asaccate with granulate exine and a subequatorial annular area that corresponds to the sexine thickness. Sculpturing consists of irregularly dispersed granules that are sometimes fused to each other (A. angustifolia) or forming microrugulae (A. araucana). Microgranules and microspinules are also present. The pollen wall ultrastructure is formed by a granular ectexine and lamellated endexine. Granular elements in A. angustifolia are more loosely disposed, form more interstices, and are gradually smaller towards the endexine than in A. araucana. To asses the probable relationships within the family, we compared the pollen grains of the two Araucaria species with those of other extant genera (Agathis, Wollemia) and also with fossil pollen (Araucariacites, Balmeiopsis, Cyclusphaera, Dilwynites) attributed to Araucariaceae.","PeriodicalId":342778,"journal":{"name":"Biocell : official journal of the Sociedades Latinoamericanas de Microscopia Electronica ... et. al","volume":"91 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2008-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128317580","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 23

Spherites in the midgut epithelial cells of the sugarcane borer parasitized by Cotesia flavipes. 被黄绒螟寄生的甘蔗螟虫中肠上皮细胞内的球粒。

Biocell : official journal of the Sociedades Latinoamericanas de Microscopia Electronica ... et. al Pub Date : 2008-04-01 DOI: 10.32604/BIOCELL.2008.32.061

D. Pinheiro, H. Conte, E. A. Gregório

{"title":"Spherites in the midgut epithelial cells of the sugarcane borer parasitized by Cotesia flavipes.","authors":"D. Pinheiro, H. Conte, E. A. Gregório","doi":"10.32604/BIOCELL.2008.32.061","DOIUrl":"https://doi.org/10.32604/BIOCELL.2008.32.061","url":null,"abstract":"Diatraea saccharalis, the main pest of sugarcane, has been controlled by Cotesia flavipes. Very little is known about the effect of parasitism on the host organs, including the midgut. The Lepidoptera midgut epithelium is composed of columnar, goblet, regenerative, and endocrine cells. Spherites have been described in columnar and regenerative cells of several Lepidoptera species, and presented a lot of functional meaning. We identified spherites in the midgut epithelial cells of non-parasitized D. saccharalis larvae analyzed the effect of parasitism on spherite morphology and distribution along the length of the midgut. Midgut fragments of both non-parasitized and parasitized larvae were processed for transmission electron microscopy. All the midgut epithelial cells showed spherites, but they were not preferentially located in a particular part of the cells. Parasitized larvae had more spherites, mainly in the columnar cells, than non-parasitized larvae. This observation was associated with an ionic imbalance within the insect host. Spherites were more abundant in the anterior midgut region than in other regions, which suggests that this region is involved in ion transport by intracellular and/or paracellular route. The morphological variability of spherites in the cells of parasitized larvae was related to the developmental stages of these structures.","PeriodicalId":342778,"journal":{"name":"Biocell : official journal of the Sociedades Latinoamericanas de Microscopia Electronica ... et. al","volume":"8 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2008-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121786768","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 20

Models for the recent evolution of protocadherin gene clusters. 原钙粘蛋白基因簇最近进化的模型。

Biocell : official journal of the Sociedades Latinoamericanas de Microscopia Electronica ... et. al Pub Date : 2008-04-01 DOI: 10.32604/BIOCELL.2008.32.009

Marcos Morgan

{"title":"Models for the recent evolution of protocadherin gene clusters.","authors":"Marcos Morgan","doi":"10.32604/BIOCELL.2008.32.009","DOIUrl":"https://doi.org/10.32604/BIOCELL.2008.32.009","url":null,"abstract":"The clustered protocadherins (Pcdhs) are single-pass transmembrane proteins that constitute a subfamily within the cadherin superfamily. In mammals, they are arranged in three consecutive clusters named alpha, beta, and gamma. These proteins are expressed in the nervous system and are targeted to mature synapses. Interestingly, different neurons express different subsets of isoforms; however, little is known about the functions and expression of the clustered Pcdhs. Previous phylogenetic analyses that compared rodent and human clusters postulated the recent occurrence of gene duplication events. Using standard phylogenetic methods, I confirmed the prior observations, and I show that duplications are likely to occur through unequal crossing-over events between two, and sometimes three, different Pcdh genes. The results are consistent with the fact that these genes undergo gene conversion. Recombination events between different clustered Pcdh genes appear to underlie concerted evolution through gene conversion and gene duplications through unequal crossing-over. In this work, I provided evidence that the unit of duplication of these genes in both the mouse and the human and within each cluster is the same. The unit of duplication includes the extracellular domain-coding sequence of an isoform and its promoter along with the cytoplasmic domain-coding region of the immediately upstream isoform in the cluster.","PeriodicalId":342778,"journal":{"name":"Biocell : official journal of the Sociedades Latinoamericanas de Microscopia Electronica ... et. al","volume":"21 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2008-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124393486","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ultrastructural analysis and identification of membrane proteins in the free-living amoeba Difflugia corona. 自由生活的冠状蝶变形虫膜蛋白的超微结构分析与鉴定。

Biocell : official journal of the Sociedades Latinoamericanas de Microscopia Electronica ... et. al Pub Date : 2007-08-01 DOI: 10.32604/BIOCELL.2007.31.225

M. SILVA-BRIANO, S. Martínez-Hernández, A. Adabache-Ortíz, J. Ventura-Juárez, E. Salinas, J. Quintanar

引用次数: 3

Cell-cell communication between mouse mammary epithelial cells and 3T3-L1 preadipocytes: effect on triglyceride accumulation and cell proliferation. 小鼠乳腺上皮细胞与3T3-L1前脂肪细胞之间的细胞-细胞通讯:对甘油三酯积累和细胞增殖的影响。

Biocell : official journal of the Sociedades Latinoamericanas de Microscopia Electronica ... et. al Pub Date : 2007-08-01 DOI: 10.32604/BIOCELL.2007.31.237

V. Julianelli, L. Guerra, J. Calvo

{"title":"Cell-cell communication between mouse mammary epithelial cells and 3T3-L1 preadipocytes: effect on triglyceride accumulation and cell proliferation.","authors":"V. Julianelli, L. Guerra, J. Calvo","doi":"10.32604/BIOCELL.2007.31.237","DOIUrl":"https://doi.org/10.32604/BIOCELL.2007.31.237","url":null,"abstract":"Interaction between parenchyma and stroma is essential for organogenesis, morphogenesis, and differentiation. Mammary gland has being the chosen model for developmental biologist because the most striking changes in morphology and function take place after birth. We have demonstrated a regulation of triglyceride accumulation by protein factors synthesized by normal mouse mammary gland epithelial cells (NMMG), acting on a cell line, 3T3-L1, long used as a model for adipogenesis. In this paper, we demonstrate that this inhibitory effect seems to be shared by other cells of epithelial origin but not by other cell types. We found a regulation of cell proliferation when NMMG cells are cultured in the presence of conditioned media from Swiss 3T3 or 3T3-L1 cells. We found a possible point of regulation for the mammary factor on a key enzyme of the lipid metabolic pathway, the glycerol-3-phosphate dehydrogenase. The inhibitory factor seems to have an effect on this enzyme's activity and reduces it. The results presented herein contribute to the understanding of cell-cell communication in a model of a normal mammary gland.","PeriodicalId":342778,"journal":{"name":"Biocell : official journal of the Sociedades Latinoamericanas de Microscopia Electronica ... et. al","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2007-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125893833","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

P53-Rb signaling pathway is involved in tubular cell senescence in renal ischemia/reperfusion injury. P53-Rb信号通路参与肾缺血再灌注损伤小管细胞衰老过程。

Biocell : official journal of the Sociedades Latinoamericanas de Microscopia Electronica ... et. al Pub Date : 2007-08-01 DOI: 10.32604/BIOCELL.2007.31.213

Kai-long Li, Xiaolan Du, He Yani, Zhao Lin, Yang Jv-rong, Ruihua Song, Chen Lin

{"title":"P53-Rb signaling pathway is involved in tubular cell senescence in renal ischemia/reperfusion injury.","authors":"Kai-long Li, Xiaolan Du, He Yani, Zhao Lin, Yang Jv-rong, Ruihua Song, Chen Lin","doi":"10.32604/BIOCELL.2007.31.213","DOIUrl":"https://doi.org/10.32604/BIOCELL.2007.31.213","url":null,"abstract":"OBJECTIVE\u0000To investigate the course of tubular cell senescence and expressions of p53, p21, and Rb during the late phase of ischemia/reperfusion (IRI) in the kidney, and assess the effects of the p53-Rb pathway on tubular cell senescence.\u0000\u0000\u0000METHODS\u0000Experimental models of unilateral renal IRI were used in p53 (+/+) and p53 (-/-) mice. Histological changes at the tubular level, progress of cell senescence, and the expression of Rb, p21, and/or p53 proteins in tubular cells were studied at different moments in time after IRI.\u0000\u0000\u0000RESULTS\u0000Chronic tubulointerstitial fibrosis was much more severe and widely distributed in IRI kidneys of p53 (+/+) mice in later stages than in earlier stages. Senescent tubular cells were significantly increased at 3 and 6 months after IRI. In contrast, in contralateral kidneys of p53 (+/+) mice and in both kidneys of p53 (-/-) mice, almost no senescent cells were observed at 1 and 3 months after IRI, and only a few senescent cells were detected in IRI kidneys of p53 (-/-) mice at 6 months. In mice of both genotypes, cell senescence was correlated with the expression levels of p53, p21, and Rb proteins.\u0000\u0000\u0000CONCLUSION\u0000The IRI accelerated tubular cell senescence is presumed to be one of the mechanisms of the \"long-term effect\" of IRI. Furthermore, the activation of p53-Rb signaling pathway may play a vital role in tubular cell senescence induced by IRI.","PeriodicalId":342778,"journal":{"name":"Biocell : official journal of the Sociedades Latinoamericanas de Microscopia Electronica ... et. al","volume":"435 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2007-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"131736087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 25

An ultrastructural study of spermiogenesis in two species of Sitophilus (Coleoptera: Curculionidae). 两种象虻精子发生的超微结构研究(鞘翅目:象虻科)。

Biocell : official journal of the Sociedades Latinoamericanas de Microscopia Electronica ... et. al Pub Date : 2007-08-01 DOI: 10.32604/BIOCELL.2007.31.229

K. Name, Giuliano Pagy Felipe dos Reis, S. Báo

引用次数: 13

Nitric oxide synthase activity in tissues of the blowfly Chrysomya megacephala (Fabricius, 1794). 大头金蝇组织中一氧化氮合酶的活性(fabicius, 1794)。

Biocell : official journal of the Sociedades Latinoamericanas de Microscopia Electronica ... et. al Pub Date : 2007-08-01 DOI: 10.32604/BIOCELL.2007.31.205

A. Faraldo, A. Sá-Nunes, L. Faccioli, E. Bel, E. Lello

{"title":"Nitric oxide synthase activity in tissues of the blowfly Chrysomya megacephala (Fabricius, 1794).","authors":"A. Faraldo, A. Sá-Nunes, L. Faccioli, E. Bel, E. Lello","doi":"10.32604/BIOCELL.2007.31.205","DOIUrl":"https://doi.org/10.32604/BIOCELL.2007.31.205","url":null,"abstract":"Although insects lack the adaptive immune response of the mammalians, they manifest effective innate immune responses, which include both cellular and humoral components. Cellular responses are mediated by hemocytes, and humoral responses include the activation of proteolytic cascades that initiate many events, including NO production. In mammals, nitric oxide synthases (NOSs) are also present in the endothelium, the brain, the adrenal glands, and the platelets. Studies on the distribution of NO-producing systems in invertebrates have revealed functional similarities between NOS in this group and vertebrates. We attempted to localize NOS activity in tissues of naïve (UIL), yeast-injected (YIL), and saline-injected (SIL) larvae of the blowfly Chrysomya megacephala, using the NADPH diaphorase technique. Our findings revealed similar levels of NOS activity in muscle, fat body, Malpighian tubule, gut, and brain, suggesting that NO synthesis may not be involved in the immune response of these larval systems. These results were compared to many studies that recorded the involvement of NO in various physiological functions of insects.","PeriodicalId":342778,"journal":{"name":"Biocell : official journal of the Sociedades Latinoamericanas de Microscopia Electronica ... et. al","volume":"11 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2007-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134577201","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 9

Cloning and analysis of IFRG (interferon responsive gene) in rabbit oocytes and preimplantation embryos. 兔卵母细胞和着床前胚胎干扰素应答基因IFRG的克隆与分析。

Biocell : official journal of the Sociedades Latinoamericanas de Microscopia Electronica ... et. al Pub Date : 2007-08-01 DOI: 10.32604/BIOCELL.2007.31.199

B. Qi, X. Zheng, Wenyong Li, Q. Wei, Qingxuan Chen

引用次数: 3

Hydrogen peroxide in micropropagation of Lilium. A comparison with a traditional methodology. 过氧化氢在百合微繁中的应用。与传统方法的比较。

Biocell : official journal of the Sociedades Latinoamericanas de Microscopia Electronica ... et. al Pub Date : 2006-12-01 DOI: 10.32604/BIOCELL.2006.30.497

N. Curvetto, P. Marinangeli, G. Mockel

{"title":"Hydrogen peroxide in micropropagation of Lilium. A comparison with a traditional methodology.","authors":"N. Curvetto, P. Marinangeli, G. Mockel","doi":"10.32604/BIOCELL.2006.30.497","DOIUrl":"https://doi.org/10.32604/BIOCELL.2006.30.497","url":null,"abstract":"The micropropagation of Lilium longiflorum requires adequate equipment which may not be afforded by small laboratories or producers. In this work we compared traditional methodology with a protocol that included easily available elements to sterilize materials and culture media, together with addition of hydrogen peroxide (H2O2) into the nutrient media as chemical sterilizer. A series of H2O2 concentrations (0.005, 0.010, 0.015 and 0.020% p/v) was used to control contamination during in vitro establishment and subsequent cultivation; the explant organogenic response was also examined and compared to the traditional micropropagation technique. The level of culture contamination was within acceptable limits in all treatments, though it was higher in the H2O2 treatments (40%) compared to the traditional methodology (20%). There were not significant differences in the number of bulblets per explant, and at the end of the multiplication phase, bulblets from 0.02% H2O2 treatment had greater biomass than from other treatments, indicating a beneficial effect. These bulblets also had a higher relative growth ratio with respect to the traditional method when cultivated for an additional period and showed the highest average bulblet fresh weight. It is expected that this higher bulblet mass would result in better performance during ex vitro cultivation.","PeriodicalId":342778,"journal":{"name":"Biocell : official journal of the Sociedades Latinoamericanas de Microscopia Electronica ... et. al","volume":"16 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2006-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116257521","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 12