Eurasian Journal of Biological and Chemical Sciences最新文献

Investigation of in vitro antidiabetic and antioxidant activity of hawthorn vinegar obtained from Endemic Crataegus tanacetifolia (Poir.) Pers. 从地方山楂（Crataegus tanacetifolia (Poir.) Pers.）中提取的山楂醋的体外抗糖尿病和抗氧化活性研究

Eurasian Journal of Biological and Chemical Sciences Pub Date : 2023-11-16 DOI: 10.46239/ejbcs.1228402

Feyza Akgün, Nigar Sıla Tuğlu, Yasemin Gülbahar Açil, Nuraniye Eruygur

{"title":"Investigation of in vitro antidiabetic and antioxidant activity of hawthorn vinegar obtained from Endemic Crataegus tanacetifolia (Poir.) Pers.","authors":"Feyza Akgün, Nigar Sıla Tuğlu, Yasemin Gülbahar Açil, Nuraniye Eruygur","doi":"10.46239/ejbcs.1228402","DOIUrl":"https://doi.org/10.46239/ejbcs.1228402","url":null,"abstract":"In this study, the in vitro antidiabetic, antioxidant activity and total flavonoid content (TFC) and total phenolic content (TPC) of vinegar obtained from endemic Crataegus tanacetifolia (Lam.) Pers. (Rosaceae), (hawthorn) were examined. Kemal Unlu brand hawthorn vinegar obtained from Malatya province (MS) and Temmuz Organic Farm vinegar obtained from Konya were used as study material. Their antidiabetic activity was determined by α-amylase and α-glucosidase inhibitory methods. Antioxidant activities were determined using 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and ferrous iron chelating (FCA) assays. The absorbance were read in the Elisa reader and evaluated with Excel and GraphPad programs. The MS has been found to have higher α- amylase (95.12± 3.71%) and α-glucosidase inhibitory (81.62 ± 0.33%) effects. The TS demonstrated (94.13 ± 3.85%) α-amylase and (75.35 ± 2.19%) α-glucosidase inhibitory activity, respectively. The TPC was found to be in TS (467.59 ± 6.73) mg GAE/mL MS (328.46 ± 5.50) mg GAE/mL. The TFC was found as (1.94 ± 10.36) mg CE/mL and (1.32 ± 10.96) mg CE/mL in TS and MS vinegar, respectively. The FCA was found to be in TS (33.37 ± 0.53%) MS (31.08 ± 10.87%). The DPPH radical scavenging activity was found as (73.82 ± 2.12%) in TS and (80.12 ± 4.45%) in MS. ABTS radical scavenging activity was found to be the highest in TS with (82.51± 0.78%) and in MS found as (78.65 ± 0.55%). The antidiabetic, antioxidant activity, TPC and TFC determinations of these vinegars were performed for the first time with these methods.","PeriodicalId":338101,"journal":{"name":"Eurasian Journal of Biological and Chemical Sciences","volume":"1 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139269265","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Endüstriyel amaçlı kullanılan bazı boyar maddelerin mutajenik etkilerinin Drosophila kanat benek testi ile in vitro olarak belirlenmesi 通过果蝇翅斑试验体外测定一些工业用染料的致突变效应

Eurasian Journal of Biological and Chemical Sciences Pub Date : 2023-11-05 DOI: 10.46239/ejbcs.1377815

Handan Uysal, Hatice Çeli̇k

{"title":"Endüstriyel amaçlı kullanılan bazı boyar maddelerin mutajenik etkilerinin Drosophila kanat benek testi ile in vitro olarak belirlenmesi","authors":"Handan Uysal, Hatice Çeli̇k","doi":"10.46239/ejbcs.1377815","DOIUrl":"https://doi.org/10.46239/ejbcs.1377815","url":null,"abstract":"Sentetik boyar maddeler tekstil, kozmetik, gıda, mobilya, ilaç ve otomotiv endüstrisi gibi yaygın kullanım alanları nedeniyle ticari öneme sahip endüstriyel ürünlerdir. Birçok ülkede kullanılan bu boyaların yaklaşık 10.000 çeşidi bulunmaktadır. Yıllık 700.000 ton üretim hacmine sahip olan boyar maddeler, tüm endüstriyel atık miktarının önemli bir kısmını (1/5) oluşturmaktadırlar. Tekstil endüstrisine ait deşarjların tarım alanlarına ve su kaynaklarına karışması toprak gözeneklerinin tıkanıp verimin düşmesine, içme ve sulama suyunun insan tüketimi için elverişsiz hale gelmesine neden olmaktadır. Bu çalışmada üç farklı tekstil boyasının (Süperfix Black NNX (SBNNX), Syanacryl Black XFDL (SBXFDL) ve Reaktive Blue 19 (RB19) mutajenik ve rekombinojenik etkileri in vivo olarak Drosophila kanat benek testi ile belirlenmiştir.Bu amaçla distile su negatif ve EMS pozitif kontrol grupları hazırlanmıştır. Ayrıca uygulama grupları için de farklı dozlarda her üç tekstil boyası (150, 300 ve 450 ppm) kullanılmıştır. Elde edilen verilere göre, tüm tekstil boyaları hem mutajenik hem de rekombinojenik etkili bulunmuştur. Uygulama gruplarına ait veriler, distile su kontrol grubu ile karşılaştırıldığında aralarındaki fark istatistiki olarak anlamlıdır (p SBXFDL > RB19 olarak belirlenmiştir.","PeriodicalId":338101,"journal":{"name":"Eurasian Journal of Biological and Chemical Sciences","volume":"41 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139288870","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Application of a new inhibitor for the corrosion of iron in acidic solution: Electrochemical effect of a scorpion venom 一种新抑制剂在酸性溶液中对铁腐蚀的应用：蝎子毒液的电化学效应

Eurasian Journal of Biological and Chemical Sciences Pub Date : 2023-11-05 DOI: 10.46239/ejbcs.1373150

D. Özkır, O. Seyyar

{"title":"Application of a new inhibitor for the corrosion of iron in acidic solution: Electrochemical effect of a scorpion venom","authors":"D. Özkır, O. Seyyar","doi":"10.46239/ejbcs.1373150","DOIUrl":"https://doi.org/10.46239/ejbcs.1373150","url":null,"abstract":"In this study, the venom of the species Leiurus abdullahbayrami, known as the yellow scorpion from the Buthidae family, was applied for the first time as a natural corrosion inhibitor for iron in hydrochloric acid solution. The effectiveness of scorpion venom as an eco-friendly and natural inhibitor was determined by electrochemical methods such as electrochemical impedance spectroscopy (EIS), potentiodynamic polarization (Tafel extrapolation method) and linear polarization resistance (LPR) after an hour of immersion. Four different concentrations were determined for the green and natural inhibitor scorpion venom in 1.0 M HCl, and it was observed that the corrosion of iron in these solutions was significantly inhibited. In general, the inhibition efficiency was above 80%. According to the potentiodynamic polarization data, it has been determined that the Leiurus abdullahbayrami venom acts as a cathodic-type inhibitor on the Fe surface. Finally, the surface images of the iron electrodes in 1.0 M HCl solutions without and with Leiurus abdullahbayrami venom after 1 h immersion were examined by field emission scanning electron microscope (FE-SEM), it was concluded that the surface containing scorpion venom had a flatter compared to the uninhibited surface.","PeriodicalId":338101,"journal":{"name":"Eurasian Journal of Biological and Chemical Sciences","volume":"119 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139289180","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparison of glutathione peroxidase-1 in free divers with their counterparts: A model study for sports informatics 自由潜水员与同龄人谷胱甘肽过氧化物酶-1 的比较：体育信息学模型研究

Eurasian Journal of Biological and Chemical Sciences Pub Date : 2023-10-18 DOI: 10.46239/ejbcs.1251033

Levent Çavaş, Elif Ci̇reli̇, Osman Ateş

引用次数: 0

Possible inhibitory effects of hoslundal, hoslundin and hoslunddiol on human lactate dehydrogenases: a bioinformatics proof 细辛、细辛定和细辛二醇对人类乳酸脱氢酶的可能抑制作用：生物信息学证明

Eurasian Journal of Biological and Chemical Sciences Pub Date : 2023-09-09 DOI: 10.46239/ejbcs.1281018

Yagmur Bi̇lgi̇n, Yasir Yalnizoğlu, Levent Çavaş

{"title":"Possible inhibitory effects of hoslundal, hoslundin and hoslunddiol on human lactate dehydrogenases: a bioinformatics proof","authors":"Yagmur Bi̇lgi̇n, Yasir Yalnizoğlu, Levent Çavaş","doi":"10.46239/ejbcs.1281018","DOIUrl":"https://doi.org/10.46239/ejbcs.1281018","url":null,"abstract":"The development of anti-malarial drugs is of great importance due to the detrimental effects of this disease all around the world. In recent years, bioinformatics tools provide considerable contributions to develop new small molecules which have important bioactivities against many bio-targets. However, biases in the methodologies or aims of the studies in which in silico tools are used may reveal problematic cases. Hoslundal, hoslundin, and hoslunddiol were proposed by Shadrack et al. (2016) to inhibit Plasmodium falciparum lactate dehydrogenase (Pf-LDH) to fight malaria. But these molecules may have potential to inhibit mammalian LDHs. To investigate whether these molecules have inhibitions on mammalian LDHs or not, we studied a comprehensive and comparative molecular docking studies as described in the present paper. According to the results, the vina scores of hoslundal without NADH for Pf-LDH, HM-LDH, HH-LDH were found as -7.5, -7.6 and -8.2 kJ/mol, respectively. Moreover, multiple sequence alignment analysis reveals high similarities among sequences. In the light of molecular studies, hoslundal were found to be connected to Pf-LDH, HM-LDH, HH-LDH (31, 26, 34), (2, -7, 154), (11, 41, 54), respectively. In conclusion, novel small molecules which are developed via in silico tools could show excellent activities against bio-targets of the pathogenic microorganisms. However, it should not be forgotten that active site of the enzymes is conserved, therefore, after a possible proposal of small molecule, its molecular docking and also Swiss-ADME studies should be necessarily carried out.","PeriodicalId":338101,"journal":{"name":"Eurasian Journal of Biological and Chemical Sciences","volume":"33 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139341521","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Makro gözenekli karbonun peroksidaz mimik aktivitesi 大孔碳的过氧化物酶模拟活性

Eurasian Journal of Biological and Chemical Sciences Pub Date : 2023-08-31 DOI: 10.46239/ejbcs.1215182

Bekir Çakiroğlu

引用次数: 0

Contributions to the fauna of Geometridae in the north eastern provinces 对东北三省尺蠖科动物的贡献

Eurasian Journal of Biological and Chemical Sciences Pub Date : 2023-08-14 DOI: 10.46239/ejbcs.1291057

M. Koyuncu, Murat Kütük, Mehmet Yaran

引用次数: 0

Kırıkhan–Reyhanlı Bölgesi Tarım Topraklarının Molibden İçeriği ve Topraktaki Bazı Ağır Metaller ile İlişkilerinin Belirlenmesi 克勒克汗-雷汉勒地区农业土壤中钼含量的测定及其与土壤中某些重金属的关系

Eurasian Journal of Biological and Chemical Sciences Pub Date : 2023-08-08 DOI: 10.46239/ejbcs.1320487

M. Yalçin

{"title":"Kırıkhan–Reyhanlı Bölgesi Tarım Topraklarının Molibden İçeriği ve Topraktaki Bazı Ağır Metaller ile İlişkilerinin Belirlenmesi","authors":"M. Yalçin","doi":"10.46239/ejbcs.1320487","DOIUrl":"https://doi.org/10.46239/ejbcs.1320487","url":null,"abstract":"Bu çalışmada Hatay ili Kırıkhan-Reyhanlı bölgesi tarım topraklarının molibden içeriğinin belirlenmesi ve toprak içerisindeki bazı ağır metaller ile ilişkilerinin saptanması amaçlanmıştır. Bu amaç için Kırıkhan-Reyhanlı bölgesi tarım topraklarını temsil edecek şekilde iki farklı derinlik (0-20 ve 20-40 cm) ve 30 ayrı noktadan olmak üzere toplamda 60 toprak örneği alınmıştır. Alınan toprak örneklerinde; Kadmiyum (Cd), Cobalt (Co), Krom (Cr), Nikel (Ni), Bakır (Cu), Demir (Fe) ve Molibden (Mo) içerikleri belirlenmiştir. Araştırma sonuçlarına göre; toprakların Cd içerikleri 0.009-0.041 µg/kg; Co içeriği 0.011-0.317 µg/kg; Cr 0.008-0.187 µg/kg, Ni içerikleri 0.787-6.211 ppm; Cu içerikleri 1.11-3.77 ppm; Fe içerikleri 2.80-15.09 ve Mo içerikleri 0.006-0.101 µg/kg arasında bulunmuştur. Toprakların Mo ile Co ve Ni içerikleri arasında pozitif önemli ilişkiler belirlenirken Cr içeriği ile negatif önemli ilişkiler belirlenmiştir. Aynı zamanda Cd ile Co ve Ni; Co ile Ni ve Cu ile Fe aralarında ise pozitif önemli ilişkiler belirlenmiştir. Bölge topraklarının ağır metal içerikleri sınır değerler ile karşılaştırıldığında herhangi bir ağır metal kirliliğine rastlanmamıştır.","PeriodicalId":338101,"journal":{"name":"Eurasian Journal of Biological and Chemical Sciences","volume":"7 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139351348","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Moringa oleifera bitki yaprağının mineral ve yağ asidi bileşenlerinin belirlenmesi

Eurasian Journal of Biological and Chemical Sciences Pub Date : 2023-06-27 DOI: 10.46239/ejbcs.1227956

Serpil Kiliç, Murat Kiliç

引用次数: 0

High Performance Liquid Chromatography with Size Exclusion Column (HPLC-SEC) Method for Identifying the Major Whey Proteins of Whey Protein Products 乳清蛋白产品中主要乳清蛋白的高效液相色谱-隔离柱鉴别方法

Eurasian Journal of Biological and Chemical Sciences Pub Date : 2023-06-27 DOI: 10.46239/ejbcs.1143057

L. Diamante

{"title":"High Performance Liquid Chromatography with Size Exclusion Column (HPLC-SEC) Method for Identifying the Major Whey Proteins of Whey Protein Products","authors":"L. Diamante","doi":"10.46239/ejbcs.1143057","DOIUrl":"https://doi.org/10.46239/ejbcs.1143057","url":null,"abstract":"This study was carried-out: a) to develop a high-performance liquid chromatography with size exclusion column (HPLC-SEC) method for the identification of the major whey proteins from selected whey protein products; and b) use the method to estimate the relative composition of the major whey proteins in different whey protein products. \u0000An HPLC Shimadzu (LC-10AD VP liquid chromatograph) with system controller (SCL-10A VP) equipped with a pump and auto-injector (SIL-10AD VP) and UV-vis detector (SPD-10AV) was used in the identification of whey proteins in standards and whey protein products. The size exclusion column (SEC) was a Yarra 3 µm, SEC-3000 Column, 7.8 mm I.D. x 30 cm with a security guard. \u0000The HPLC-SEC method was successful in identifying the major whey proteins of the different whey protein products. \u0000The β-LG contents had the highest level among the whey proteins for all the whey protein products studied, followed by the α-LA and then IgG in both WPC products. However, the α-LA and IgG of the Procream product had almost the same level which was probably due to a different process used in WPC \u0000All the major whey proteins with the highest pump flowrate had the shortest elution times while the whey proteins with the lowest pump flowrate had the longest elution times. The optimal pump flowrate was 0.75 mL/min since it gave a faster analysis but differentiate the peaks of the different major whey proteins.","PeriodicalId":338101,"journal":{"name":"Eurasian Journal of Biological and Chemical Sciences","volume":"55 80 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124774504","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0