Theoretical and Applied Veterinary Medicine最新文献

{"title":"The effectiveness of Imrovac® in industrial pork production","authors":"V. V. Samoilіuk, D. Bilyi, M. Koziy, S. Maslikov, Т. L. Spitsina","doi":"10.32819/2021.91001","DOIUrl":"https://doi.org/10.32819/2021.91001","url":null,"abstract":"The clinical efficacy of the use of immunobiological castration was studied in comparison with boars in which orchidectomy was performed by open method «on a ligature», as well as in non-castrated animals. It was found that the disadvantage of surgical castration is the risk of bleeding, the development of purulent inflammatory process (edges of the surgical wound, scrotal tissues, stump, common vaginal membrane), as well as intra- and postoperative hernias, which are accompanied by prolapse of the omentum, intestines, and bladder. The low incidence of these complications is offset by the high death rate caused by them. A relatively high level of cryptorchidism was established on the farm, among which bilateral was 27.6%. As a result of the use of immunological castration, in comparison with surgical castration, and with non-castrated boars, the average daily gains were increased, the fattening period was reduced and feed costs were reduced against the background of feed conversion increase by 14 – 27%. The use of Improvac® (Pfizer Ltd) led to better assimilation of feed, an increase in the amount of meat on the carcass, in particular, lean meat by 12 – 16%, lumbar depth, the protein content in meat and a decrease of the fat percentage and fat thickness, eliminating specific odor, and reduction in the fattening period, as well as costs for feeding. In particular, compared to non-castrated boars, the weight of chilled half carcasses increased by 1.2 times (P < 0.05). Immunological castration led to atrophy of the testicles: their weight, length and width were smaller compared to non-castrated boars, which again confirms a decrease in the level of boars’ sex hormones. It is important to use Improvac® in breeding boars that have exhausted their potential, eliminating the need to keep them for about three months to reduce their meat and lard smell. Reducing the period of disappearance of the odor of products obtained from boars led to a reduction in the cost of feeding, maintenance, and veterinary care. Production indicators justify the economic feasibility of using immunological castration with Improvac® in order to obtain additional income, given the absence of probable complications associated with the conventional surgical procedure.","PeriodicalId":33793,"journal":{"name":"Theoretical and Applied Veterinary Medicine","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70175072","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Modern concept of physiological signaling systems in cattle fetuses with the participation of FC-γ-receptors","authors":"D. M. Masіuk","doi":"10.32819/2021.92010","DOIUrl":"https://doi.org/10.32819/2021.92010","url":null,"abstract":"The article presents a literature review on the modification of expression and recycling of Fc-γ-receptors of fetal jejunal enterocytes in cattle. Based on the analyzed data, a modern concept of physiological signaling systems in cattle fetuses with the participation of Fc-γ receptors has been developed. These results indicated age-related modulation of FcγR intestinal cells expression during the entire fetal period of cattle, and the dynamics of changes in the content of polypeptides with different molecular weights that exhibit Fc-γ-binding activity in the basolateral and apical regions of the enterocyte’s plasma membrane and have certain characteristic features for each groups of receptors for IgG. It has been proven that the regulation of FcγR expression by plasma membrane of jejunal enterocytes in cattle during the fetal period of ontogenesis is controlled by mechanisms associated with fetal development. The obtained research results and their in-depth analysis made it possible to propose a scientific concept on the physiological functions of FcγR during the fetal period of cattle ontogenesis. In particular, they translocate signals by the mother-placenta-fetus chain, form one of the key signaling systems for regulating the development of enterocytes, recognize specific signals from immunoglobulins and antigens, play an important role in the transcytosis and recycling of IgG from the amniotic fluid into fetal circulation, and form the immune mechanisms of the fetus for the intrauterine functioning body adaptation and prepare it for antigenic pressure after birth. So, the expression modulation, localization and identification of polypeptides on the plasma membrane that exhibit Fc-γ-binding activity, makes it possible to form a signaling system and control the development of barrier and immune functions in the fetus with the participation of FcγR.","PeriodicalId":33793,"journal":{"name":"Theoretical and Applied Veterinary Medicine","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70175178","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effect of humic substances on carbohydrate metabolism in alloxan-induced diabetes mellitus in rats","authors":"V. Utkina, L. Stepchenko","doi":"10.32819/2021.92015","DOIUrl":"https://doi.org/10.32819/2021.92015","url":null,"abstract":"The article presents study results of the effect of humic nature biologically active additive «Humilid» on carbohydrate metabolism, namely on glucose, insulin and C-peptide in alloxan-induced diabetes mellitus in rats. The purpose of the experiment was to investigate how “Humilid” affects carbohydrate metabolism in rats; to study and analyze the effect of the humic nature biologically active substance «Humilid» on metabolic processes. In the work we used biochemical methods for studying the blood of experimental rats. Adult male white rats were taken for the experiment, from which four groups were formed. The experiment lasted three weeks, during which the animals from the first experimental group received daily in addition to the main feed an aqueous solution of feed additive «Humilid» at dose 5 mg / kg of body weight of the active substance, and had free access to water. Rats of the second and third groups were injected with alloxan as a single intraperitoneal injection. The rats of the third group additionally received an aqueous solution of feed additive «Humilid». It was identified that “Humilid” has an antioxidant activity, which may be one of the mechanisms of its antidiabetic action, as it is known that alloxan causes free radical damage to β-cells with weak antioxidant protection. The use of a biologically active feed additive of humic nature was found to have a hypoglycemic effect in both healthy animals as well as in rats with alloxan-induced diabetes mellitus. It was found that the use of “Humilid” in rats enhances energy processes in their bodies of experimental animals. There was a decrease in glucose content in rats from second group by 11.9% (P ≤ 0.001) compared with the control one. At the same time, the glucose content in animals from third and fourth groups was higher by 35.9% (P ≤ 0.001) and 20% (P ≤ 0.001) higher than in animals from the control one. The insulin concentration in the blood of rats from the second group was 7.4% higher (P ≤ 0.05) compared to the animals from first group. The concentration of C-peptide was within the reference values. The levels of insulin and C-peptide concentration in animals of the third and groups were lower by 47.8% (P ≤ 0.001) and 32.4% (P ≤ 0.001) and 55.5% (P ≤ 0.001) and 37.5% (P ≤ 0.001) in comparison with animals of first group. A pronounced hypoglycemic effect, which was due to an increase in the sensitivity of tissues to glucoregulatory hormones and an increase in the body’s tolerance to excessive intake of carbohydrates. The obtained results of the study indicate the regulatory effect of humic feed additive «Humilid» on key links in the regulation of carbohydrate metabolism, which is characterized by the decrease in blood glucose level due to activation of the synthesis and secretion of insulin and C-peptide.","PeriodicalId":33793,"journal":{"name":"Theoretical and Applied Veterinary Medicine","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70175188","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Increase of the growth rate in young pigs while using the feed additive «Natufactant»","authors":"N. Begma, O. Musich","doi":"10.32819/2021.91006","DOIUrl":"https://doi.org/10.32819/2021.91006","url":null,"abstract":"The improvement of production indicators (feed conversion ratio, average daily body weight gain, growth rate, etc.) in animal husbandry implies a high energy requirement. Such a need can only be met by including fats in the diet. Fat is the main and most efficient source of feed energy, but at the same time, one of the most expensive feed component. Using a fat emulsifier is an increase in the quality of the obtained product and a decrease in its cost. The addition of synthetic emulsifiers is a relatively new opportunity to increase fats’ active surface compared to other widely used feed additives. This reduces the cost for oil in the feed and also a chance to reduce feed total cost. By increasing fats’ active surface, emulsifiers expand the action of lipases and promote micelles formation. Higher the fat percentage in the diet, lower the percentage of its absorption. In high-energy diets without the use of complex emulsifiers, from 36% to 70% of the fat introduced into the feed is not absorbed and is excreted from the body. The beneficial effect of emulsifiers is that the digestibility of fat decreases, and it grows with an increase in the fat level in the diet. An assessment of the effect of the «Natufactant» feed additive on the growth rates of young pigs has been carried out. The maintenance and feeding of pigs were carried out in accordance with the technology adopted on the farm. The nutritional value of the main diet that is used on the farm, as well as after the inclusion of the feed additive «Natufactant», were determined. To conduct research according to the principle of analogs were taking into account the breed, live weight, and general physiological state, two groups of animals of a large white pig breed were created, 25 animals in each, with a live weight of 28 kg. The first group served as a control, and in addition to the main diet, the multicomponent emulsifier «Natufactant» was administered to the piglets of the research group at a dose of 250 g/t of feed, which was given together with the feed once per day. It was found that high average daily gains were obtained from animals to which «Natufactant» was added at the rate of 250 g per 1 ton of compound feed from the first day of the experiment and for 60 days in a row. As a result of the production check, it was found that the introduction of a feed additive into the diet of young pigs made it possible to increase the gross increase in animals’ live weight in comparison with the control, by 10.7 centners with the same level of feeding. New influence aspects of the fat emulsifier usage on the pigs’ physiological state and productivity were disclosed. It has been proven that the inclusion of the «Natufactant» feed additive into the animals’ diet stimulates the digestion processes and assimilation of basic nutrients, improves their physiological state, and increases the average daily weight gain of pigs by 15.3%. The solution to the problem of fats’ digestibility is the creation of ","PeriodicalId":33793,"journal":{"name":"Theoretical and Applied Veterinary Medicine","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70175365","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cytomorphological changes of a cat’s cadaver’s parenchymal organs in the early postmortem period in the forensic veterinary examination aspect","authors":"R. Kazantsev, I. Yatsenko","doi":"10.32819/2021.93023","DOIUrl":"https://doi.org/10.32819/2021.93023","url":null,"abstract":"The cytomorphological changes of a cat’s cadaver’s parenchymal organs in the early postmortem period in the aspect of forensic veterinary examination are established in the work. The aim of the work is to clarify the processes of necrobiosis at the cellular level under aerobic environment in the first day after the death of the animal as a cytomorphological criterion in terms of forensic veterinary examination in deciding the age of death in the early postmortem period. It is proved that the most dynamic cytomorphological changes are found in the spleen and pancreas tissue; cytoarchitectonics of liver, lung and heart tissue changes less intensively. Intense cytomorphological changes in kidney tissue and brain were not observed. It was found that parenchymal organs samples cytological research after the first day of the postmortem period to determine the cells histological topography is impossible. It was found that the cytoarchitectonics dynamics of kidney and brain tissue is uninformative to solve the problems of forensic veterinary examination, but the necrobiosis of liver, lung and heart cells is moderately informative. High informativeness of cytomorphological picture change in spleen and pancreas tissue has been established, as the intensity of cytomorphological changes dynamics and their informativeness to solve the question of the age of death are directly correlated. It is recommended to use the cytological rapid method as an additional in the practice of forensic veterinary examination to address the issue of animal death age in terms of its non-obviousness before the appearance of late cadaveric phenomena.","PeriodicalId":33793,"journal":{"name":"Theoretical and Applied Veterinary Medicine","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70175566","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Limiting factors of cows’ productive longevity on the industrial complex","authors":"I. Pishchan, S. Pishchan, L. Lytvyschenko, N. Kapshuk, H. S. Hutsuliak","doi":"10.32819/2021.93019","DOIUrl":"https://doi.org/10.32819/2021.93019","url":null,"abstract":"The research was carried out in the conditions of a large industrial complex ‟Agro-Soyuz” of the Dnipropetrovsk region for the production of the milk from Holstein cows. It has been established that due to ineffective management decisions in the industrial complex, animals leave the herd annually because of metabolic disorders and operational risks with certain dynamics depending on age. It was found that the stimulation of lactogenic function in cows with an increased rate of concentrated feed causes some metabolic disorders, which account for 30.4% of the total number of non-infectious diseases and animals retirement from the herd. It is characteristic that metabolic disorders are observed almost to the same extent in first-calf heifers and in older lactation cows. At the same time, the displacement of the abomasum in animals of the third and fourth lactations under the conditions of an industrial complex was not observed, and depletion of the body (cachexia) was characteristic of first-calf heifers, which among metabolic disorders occupied 33.3%. Cows of the fourth lactation did not suffer from enteritis. Because of adipose tissue purulent inflammation (phlegmon), an average of 5.92% of cows were eliminated from the herd. As the animals age, there was an increase in extremities disease and a decrease in the incidence of hypogalactia. It has been proven that first-calf heifers were more sensitive to mastitis of the udder than older animals. Young cows left the herd due to mastitis at the level of 17.16%, while for hypogalactia - only 7.5%. Through exploitation risks, an average of 27.04% of animals are leaving the herd annually, and this indicator grows with age: if the first-heifers are leaving at the level of 23.13%, then the cows of the second lactation were culled at the level of 28.57%, the third and fourth – respectively 30.56 and 29.41%.","PeriodicalId":33793,"journal":{"name":"Theoretical and Applied Veterinary Medicine","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70175713","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of the death causes in sturgeon fish on a farming environment","authors":"N. Hubanova, R. Novitskiy, A. Horchanok, L. Bajdak, N. Prysiazhniuk","doi":"10.32819/2021.93024","DOIUrl":"https://doi.org/10.32819/2021.93024","url":null,"abstract":"Artificial breeding of sturgeon fish ensures the preservation of the species as a component of the organic world system, restoration of the population and obtaining a high-quality and valuable fish product. The fact of a significant number death of Siberian (Lena) sturgeon (Acipenser baerii) raised on a farm (Dnipropetrovsk region, Ukraine) was investigated. A complex of hydrochemical, ichthyological, hydrobiological, mycological and microbiological research methods was used. It was found that due to the violation of the technological conditions for keeping sturgeon fish in the enterprise, there were changes in their behavior, peculiarities in movement, loss of appetite with the death of a significant number of fish. An increase in water temperature by two degrees, technical work nearby the pools led to a deterioration in water quality, a change in the behaviour of fish and its death. This case is an example of the lack of education in employees from enterprise, the economy of the owner’s funds: the norms for planting fish of the third and fourth years of life have been violated. No infectious diseases were found in the fish pools. As a result of this work, recommendations were made on the conditions of keeping sturgeons, maintaining the water quality at the proper level, using various types of feed and vitamins to restore the physiological state of animals and to prevent the occurrence of such situations in the future.","PeriodicalId":33793,"journal":{"name":"Theoretical and Applied Veterinary Medicine","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70175685","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Slaughter animals’ meat identification according to suitability for consumption using developed methods","authors":"N. Bogatko, T. Mazur, А. F. Bogatko","doi":"10.32819/2021.91002","DOIUrl":"https://doi.org/10.32819/2021.91002","url":null,"abstract":"It is recommended for state veterinary inspectors simple, improved and new methods for identifying meat of slaughter animals according to suitability for consumption and compliance with its shelf life due to the detection of the peroxidase enzyme in the filtered meat-water extract; the use of an improved formol reaction at the facilities for meat production and turnover as a result of the substitution of suitable meat for human consumption with meat from sick animals, having reliability in tests of 99.8% and 96.4%, respectively. And also a new method of bacterioscopic assessment of the degree of bacterial contamination of the meat from slaughter animals by staining smears-prints according to Gram in Hooker’s modification was developed and recommended to establish the degree of freshness, which has a reliability of 99.5%. It was found that the largest percentage of meat was obtained from healthy animals with an improved method for detecting the peroxidase enzyme at meat production facilities − from 60.00 to 89.58%, at wholesale depots − from 61.54 to 81.82%; when sold in supermarkets − from 56.52 to 90.48%; in agriood markets − from 69.70 to 78.26%. However, the percentage of meat obtained from suspiciously sick animals at its production facilities ranged from 9.52 to 30.00%, at wholesale depots − from 13.63 to 26.92%; when sold in supermarkets − from 9.52 to 27.27%, in agrifood markets − from 8.70 to 21.21%; and meat obtained from sick animals, respectively, at production facilities and wholesale depots − from 4.76 to 10.00% and from 4.55 to 11.54%; in supermarkets and agrifood markets, respectively − from 4.76 to 17.39% and from 9.09 to 13.04%. It was found that the largest percentage of meat was obtained from healthy animals with an improved formol reaction method at meat production facilities − from 72.73 to 93.18%, at wholesale depots for storage − from 69.56 to 77.78%, when sold in supermarkets − from 67.39 to 78.26%, and in agrifood markets − from 75.00 to 78.57%. Although, the percentage of meat obtained from suspiciously sick animals at the facilities for its production ranged from 6.82 to 18.18%, at wholesale depots − from 16.67 to 26.09%; when sold in supermarkets ranged from 13.04 to 23.91%; in agrifood markets − from 15.28 to 20.00%; and meat obtained from seriously ill animals at at meat production facilities and wholesale depots, respectively, from 3.23 to 9.09% and from 4.35 to 8.33%; in supermarkets and agrifood markets, respectively − from 6.98 to 8.70% and from 4.76 to 7.69%.","PeriodicalId":33793,"journal":{"name":"Theoretical and Applied Veterinary Medicine","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70175080","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Seasonal effect on milk productivity and cases of mastitis in Ukrainian Brown Swiss Cows","authors":"R. Mylostyvyi, O. Izhboldina, O. Кalinichenko, O. Orishchuk, I. Pishchan, O. Khramkova, N. Kapshuk, P. Skliarov, V. Sejian, G. Hoffmann","doi":"10.32819/2021.92011","DOIUrl":"https://doi.org/10.32819/2021.92011","url":null,"abstract":"Seasonality affects milk production, its composition, as well as the spread of mastitis in dairy cows. The aim of the research work was to study the way the seasons affect milk productivity and the manifestation of mastitis among Ukrainian brown Swiss cows at a commercial dairy unit, with the animals kept in naturally ventilated premises. The relationship between the indicators was assessed by Spearman’s rank-order correlation coefficient. The influence of a seasonal factor and individual air parameters on cows’ milk productivity was evaluated using Factorial ANOVA in Statistica 12 software. The results of the study revealed a high correlation between the content of milk fat and milk protein and the weather conditions (temperature, relative humidity, as well as temperature-humidity index) by seasons. Moreover, the greatest negative relationship between these characteristics was observed in spring (r = 0.4‒0.8) and in autumn (r = 0.6), and not in summer during the heat, as we had predicted earlier. The influence rate of the «season» factor was significant both in terms of the daily milk yield and milk components (51–59%) and mastitis prevalence rate in cows (56%) at the dairy unit. In general, the reduction of milk yields in summer and especially in autumn, and the spread of udder pathology in cows during this period should provide for the introduction of managerial and preventive veterinary measures to mitigate the effects of hot summer among Ukrainian Brown Swiss cows at year-round housing of animals in naturally ventilated premises.","PeriodicalId":33793,"journal":{"name":"Theoretical and Applied Veterinary Medicine","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70175234","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Species composition of microorganisms isolated from biomaterials of sick calves and cows","authors":"O.E. Ayshpur, I. Mushtuk, V. V. Gumeniuk, О. М. Yermolenko, V. Hlebeniuk","doi":"10.32819/2021.94027","DOIUrl":"https://doi.org/10.32819/2021.94027","url":null,"abstract":"Bacteriosis of cattle, the main cause of which is opportunistic microflora, cause significant damage to the livestock industry. Therefore, the study of the etiological structure of bacteriosis in cattle is relevant. During 2018, 2020–2021, 824 biomaterials’ samples of from 97 livestock farms in different regions of Ukraine were studied, including milk samples from patients with mastitis and vaginal lavages during cow endometritis, nasal mucus from calves with respiratory syndrome, feces from animals with diarrhea, exudate during inflammatory processes of the extremities. During bacteriological studies of biomaterials, 35 types of microorganisms were isolated. It should be noted that from 23.7% to 24.2% of Escherichia coli isolates were isolated; bacteria of the genus Proteus – from 10.8% to 16.7%. The important role of staphylococci in the occurrence of cattle bacteriosis has been proved, they have been isolated from 21.43% to 28.75%, while the proportion of Staphylococcus aureus isolation increased up to 21.6%. In the course of the biomaterials studies, up to 16.3% of enterococci were identified. According to our long-term research, simultaneous or sequential infection of animals with two or more pathogens has been established. Over the last year of observations (2021) the same trend was revealed towards an increase in the cases of two or more pathogens detection from one animals’ biomaterial with bacteriosis. If one is isolated in 31.11% of samples, while two – in 39.68%, three – in 18.41%.","PeriodicalId":33793,"journal":{"name":"Theoretical and Applied Veterinary Medicine","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70175588","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}