S. Sarker, Begum Fatema Zohara, Azizunnesa, M. Islam, F. Bari
{"title":"Testicular and epididymal ultrasonography for the assessment of semen quality in the indigenous ram","authors":"S. Sarker, Begum Fatema Zohara, Azizunnesa, M. Islam, F. Bari","doi":"10.12750/jarb.36.4.212","DOIUrl":"https://doi.org/10.12750/jarb.36.4.212","url":null,"abstract":"The aims of this study were to measure the ultrasonographic biometry of genitalia of the indigenous rams and observe the relationship of biometry on semen parameters. The epididymal volume was significantly reduced (p < 0.01) after semen collection compared with before collection for both left and right part in all rams. The cumulative results showed that although there was no significant difference in length, width and volume of epididymis between before and after semen collection, however the values were lower after collection. The epididymal length was significantly correlated with epididymal volume (p < 0.01), semen motility (p < 0.05) and semen morphology (p < 0.01). Epididymal width was only significantly correlated with epididymal volume (p < 0.01) not with the semen parameters. Epididymal volume had a significant correlation only with semen morphology (p < 0.01).The scrotal circumference had the significant correlation with semen density, mass activity, concentration and motility (p < 0.01). The epididymis had the similar or slightly increased echogenicity as compared to the normal testis. During whole study, some white spots were found on testis which did not affect the semen quantity and quality. Significant variation was observed only for semen concentration and motility among the rams (p < 0.05). The overall normal morphology was 90.5 ± 4.6% with highest percentage of coiled tail abnormalities.","PeriodicalId":33755,"journal":{"name":"Journal of Animal Reproduction and Biotechnology","volume":"17 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76078061","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Case report of amniotic membrane derived-stem cells treatment for feline chronic obstructive pulmonary disease","authors":"Taeho Kim, S. Noh, J. Ju","doi":"10.12750/jarb.36.4.323","DOIUrl":"https://doi.org/10.12750/jarb.36.4.323","url":null,"abstract":"","PeriodicalId":33755,"journal":{"name":"Journal of Animal Reproduction and Biotechnology","volume":"36 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83152870","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Effects of Mito-TEMPO on the survival of vitrified bovine blastocysts in vitro","authors":"Jae-Hoon Jeong, S. Yang, Hyo-Jin Park, D. Koo","doi":"10.12750/jarb.36.4.299","DOIUrl":"https://doi.org/10.12750/jarb.36.4.299","url":null,"abstract":"","PeriodicalId":33755,"journal":{"name":"Journal of Animal Reproduction and Biotechnology","volume":"54 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74869890","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Antioxidant effect of Lonicera Caerulea on heat stress-treated male mice","authors":"Donghun Kang, Daeyoung Kim","doi":"10.12750/jarb.36.4.220","DOIUrl":"https://doi.org/10.12750/jarb.36.4.220","url":null,"abstract":"Over the past few decades, the increasing number of infertility factors in young men worldwide and the gradual deterioration of sperm quality have led to lower sperm parameters proposed by the World Health Organization (WHO) (Cohen et al., 2015). There is growing evidence that human sperm quality is declining. Decreased sperm quality is caused by exposure to negative lifestyle habits such as obesity, cell phone use, smoking, and alcohol consumption (Dissanayake et al., 2019). Among them, the most direct cause is genital heat stress due to high testicular heat, which is a real risk factor for male infertility. The testes are the male reproductive organs responsible for sperm production. In mammals, the temperature of the testes to form normal sperm must be 2-8°C below body temperature. Heat stress is applied to the testicles in daily living environments such as prolonged sitting or exposure to radiant heat, varicose veins, latent orchiopathy, over-the-lap use of laptops, sauna, and warm baths (Durairajanayagam et al., 2015; Hamilton et al., 2016). In elevated testicular temperature, sperm stem cells are damaged, affecting the differentiation and proliferation of sperm (Ahn et al., 2020). In addition, heat stress and Antioxidant effect of Lonicera Caerulea on heat stress-treated male mice","PeriodicalId":33755,"journal":{"name":"Journal of Animal Reproduction and Biotechnology","volume":"248 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76988535","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Oral administration of Grifola frondosa affect lipid metabolism and insulin signaling pathway on BKS. Cg-+Leprdb/+Leprdb/OlaHsd mouse","authors":"Seong-Bo Yun, Dae-Young Kim","doi":"10.12750/jarb.36.4.203","DOIUrl":"https://doi.org/10.12750/jarb.36.4.203","url":null,"abstract":"Author’s Position and Orcid no. Yun SB, MS student, https://orcid.org/0000-0003-0068-0694 Kim DY, Professor, https://orcid.org/0000-0003-1608-8441 ABSTRACT Diabetic mellitus (DM) is a carbohydrate metabolic disorder that involves high blood sugar because insulin works abnormally. Type 2 diabetes accounts for most of them. However, diabetes treatments such as GLP-1 and DPP-4 inhibitors commonly caused side effects including gastrointestinal disorders. Grifola frondosa (G. frondosa) revealed various pharmacological effects in recent studies. It has a variety of anti-cancer polysaccharides through host-mediated mechanisms. D-fraction in G. frondosa has apoptotic effects, promoting myeloid cell proliferation and differentiation into granulocytes-macrophages. It has also been shown to reduce the survival rate of breast cancer cells. Though, no further study has been conducted on the specific effects of G. frondosa in the db/db mouse. Therefore, we would like to research the blood glucose improving effect of G. frondosa, a natural material, in type 2 diabetes model mouse, in this study. G. frondosa was administered to the disease model mouse (BKS.Cg-+Lepr/+Lepr/OlaHsd) for 8 weeks to monitor weight and blood glucose changes every week. And we evaluated anti-diabetes effects by checking biomarker changes shown through blood. Experiment did not show statistically significant weight differences, but control groups showed significantly higher weight gain than G. frondosa administered groups. We collected blood from the tail veins of the db/db mouse each week. As a result, the lowest blood sugar level was shown in the 500 mg/ kg group of G. frondosa. Glucose in the blood was examined with HBA1c, and 7.8% was shown in the 500 mg/kg administration group, lower than in other groups. These results suggest the potential improvements of diabetes in G. frondosa.","PeriodicalId":33755,"journal":{"name":"Journal of Animal Reproduction and Biotechnology","volume":"9 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74322617","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Junyoung Kim, Youngwook Jung, H. Jung, Muhammad Shakeel, M. Yoon
{"title":"Olfactory receptor (OR7D4 and OR1I1) expression in stallion testes","authors":"Junyoung Kim, Youngwook Jung, H. Jung, Muhammad Shakeel, M. Yoon","doi":"10.12750/jarb.36.4.292","DOIUrl":"https://doi.org/10.12750/jarb.36.4.292","url":null,"abstract":"Olfactory receptors (OR) are primarily responsible for the detection of odorant molecules. We previously demonstrated that OR7D4, an OR for androstenone, is expressed in the vomeronasal organ and olfactory epithelium tissue of stallions. Recently, the expression of OR1I1 in the human testes was reported and the possible roles of OR1I1 in the testicular cells were suggested. The objectives of this study were 1) to explore the expression of OR7D4 and OR1I1 in stallion testes, and 2) to define the specific localization of OR7D4 and OR1I1 in the testicular tissues. Stallion testicular tissue samples were used for this study. Western blot was performed to confirm the cross-reactivity of OR7D4 and OR1I1 antibody with stallion testicular tissue samples. OR7D4 and OR1I1 gene expressions were investigated using reverse transcription-polymerase chain reaction (RT-PCR) in stallion testes. Immunofluorescence was performed to investigate the expression of OR7D4 and OR1I1 in stallion testicular tissues. The protein bands for OR7D4 and OR1I1 from the testes were observed at approximately 38 kDa and 43 kDa, respectively. The mRNA of OR7D4 and OR1I1 were detected in stallion testes. Immunolabeling of OR7D4 and OR1I1 in the cytoplasm of both spermatogonia and Leydig cells was observed. In conclusion, androstenone and another odorant chemical, which is recognized by OR1I1, may play an important role in stallion testes.","PeriodicalId":33755,"journal":{"name":"Journal of Animal Reproduction and Biotechnology","volume":"68 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75701573","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Traf4 is required for tight junction complex during mouse blastocyst formation","authors":"Jian Lee, I. Choi","doi":"10.12750/jarb.36.4.307","DOIUrl":"https://doi.org/10.12750/jarb.36.4.307","url":null,"abstract":"","PeriodicalId":33755,"journal":{"name":"Journal of Animal Reproduction and Biotechnology","volume":"5 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78667495","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"CDDO-Me alleviates oxidative stress in human mesenchymal stem cells","authors":"Hye Jin Cho, Tae Min Kim","doi":"10.12750/jarb.36.4.285","DOIUrl":"https://doi.org/10.12750/jarb.36.4.285","url":null,"abstract":"Mesenchymal stem cells (MSCs) have been recognized as a therapeutic tool for various diseases due to its unique ability for tissue regeneration and immune regulation. However, poor survival during in vitro expansion and after being administrated in vivo limits its clinical uses. Accordingly, protocols for enhancing cell survivability is critical for establishing an efficient cell therapy is needed. CDDO-Me is a synthetic C-28 methyl ester of 2-cyano-3,12-dioxoolean-1,9-dien-28-oic acid, which is known to stimulate nuclear factor erythroid 2-related factor 2 (Nrf2)-antioxidant response element (ARE) pathway. Herein, report that CDDO-Me promoted the proliferation of MSCs and increased colony forming units (CFU) numbers. No alteration in differentiation into tri-lineage mesodermal cells was found after CDDO-Me treatment. We observed that CDDO-Me treatment reduced the cell death induced by oxidative stress, demonstrated by the augment in the expression of Nrf2-downstream genes. Lastly, CDDO-Me led to the nuclear translocation of NRF2. Our data indicate that CDDO-Me can enhance the functionality of MSCs by stimulating cell survival and increasing viability under oxidative stress.","PeriodicalId":33755,"journal":{"name":"Journal of Animal Reproduction and Biotechnology","volume":"11 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80661145","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
So-Yeon Jo, Yong Hwangbo, Sang-Hee Lee, H. Cheong, Dong-Ku Kim, Choon-keun Park
{"title":"Effect of antibodies binding to Y chromosome-bearing sperm conjugated with magnetic nanoparticles on bull sperm characteristics","authors":"So-Yeon Jo, Yong Hwangbo, Sang-Hee Lee, H. Cheong, Dong-Ku Kim, Choon-keun Park","doi":"10.12750/jarb.36.4.239","DOIUrl":"https://doi.org/10.12750/jarb.36.4.239","url":null,"abstract":"","PeriodicalId":33755,"journal":{"name":"Journal of Animal Reproduction and Biotechnology","volume":"77 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88560370","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Doo Kim, Ji-Hwan Lee, G. Jang, Eun-Jeong Choi, Jin-Ju Kim, Jian Lee, J. Son
{"title":"The studies on characteristics of return to estrus in postpartum dairy cattle","authors":"Doo Kim, Ji-Hwan Lee, G. Jang, Eun-Jeong Choi, Jin-Ju Kim, Jian Lee, J. Son","doi":"10.12750/jarb.36.4.230","DOIUrl":"https://doi.org/10.12750/jarb.36.4.230","url":null,"abstract":"","PeriodicalId":33755,"journal":{"name":"Journal of Animal Reproduction and Biotechnology","volume":"22 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90741335","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}