Journal of Animal Reproduction and Biotechnology最新文献_第9页

CDDO-Me alleviates oxidative stress in human mesenchymal stem cells CDDO-Me减轻人间充质干细胞氧化应激

Journal of Animal Reproduction and Biotechnology Pub Date : 2021-12-31 DOI: 10.12750/jarb.36.4.285

Hye Jin Cho, Tae Min Kim

{"title":"CDDO-Me alleviates oxidative stress in human mesenchymal stem cells","authors":"Hye Jin Cho, Tae Min Kim","doi":"10.12750/jarb.36.4.285","DOIUrl":"https://doi.org/10.12750/jarb.36.4.285","url":null,"abstract":"Mesenchymal stem cells (MSCs) have been recognized as a therapeutic tool for various diseases due to its unique ability for tissue regeneration and immune regulation. However, poor survival during in vitro expansion and after being administrated in vivo limits its clinical uses. Accordingly, protocols for enhancing cell survivability is critical for establishing an efficient cell therapy is needed. CDDO-Me is a synthetic C-28 methyl ester of 2-cyano-3,12-dioxoolean-1,9-dien-28-oic acid, which is known to stimulate nuclear factor erythroid 2-related factor 2 (Nrf2)-antioxidant response element (ARE) pathway. Herein, report that CDDO-Me promoted the proliferation of MSCs and increased colony forming units (CFU) numbers. No alteration in differentiation into tri-lineage mesodermal cells was found after CDDO-Me treatment. We observed that CDDO-Me treatment reduced the cell death induced by oxidative stress, demonstrated by the augment in the expression of Nrf2-downstream genes. Lastly, CDDO-Me led to the nuclear translocation of NRF2. Our data indicate that CDDO-Me can enhance the functionality of MSCs by stimulating cell survival and increasing viability under oxidative stress.","PeriodicalId":33755,"journal":{"name":"Journal of Animal Reproduction and Biotechnology","volume":"11 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80661145","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Olfactory receptor (OR7D4 and OR1I1) expression in stallion testes 种马睾丸嗅觉受体OR7D4和OR1I1的表达

Journal of Animal Reproduction and Biotechnology Pub Date : 2021-12-31 DOI: 10.12750/jarb.36.4.292

Junyoung Kim, Youngwook Jung, H. Jung, Muhammad Shakeel, M. Yoon

{"title":"Olfactory receptor (OR7D4 and OR1I1) expression in stallion testes","authors":"Junyoung Kim, Youngwook Jung, H. Jung, Muhammad Shakeel, M. Yoon","doi":"10.12750/jarb.36.4.292","DOIUrl":"https://doi.org/10.12750/jarb.36.4.292","url":null,"abstract":"Olfactory receptors (OR) are primarily responsible for the detection of odorant molecules. We previously demonstrated that OR7D4, an OR for androstenone, is expressed in the vomeronasal organ and olfactory epithelium tissue of stallions. Recently, the expression of OR1I1 in the human testes was reported and the possible roles of OR1I1 in the testicular cells were suggested. The objectives of this study were 1) to explore the expression of OR7D4 and OR1I1 in stallion testes, and 2) to define the specific localization of OR7D4 and OR1I1 in the testicular tissues. Stallion testicular tissue samples were used for this study. Western blot was performed to confirm the cross-reactivity of OR7D4 and OR1I1 antibody with stallion testicular tissue samples. OR7D4 and OR1I1 gene expressions were investigated using reverse transcription-polymerase chain reaction (RT-PCR) in stallion testes. Immunofluorescence was performed to investigate the expression of OR7D4 and OR1I1 in stallion testicular tissues. The protein bands for OR7D4 and OR1I1 from the testes were observed at approximately 38 kDa and 43 kDa, respectively. The mRNA of OR7D4 and OR1I1 were detected in stallion testes. Immunolabeling of OR7D4 and OR1I1 in the cytoplasm of both spermatogonia and Leydig cells was observed. In conclusion, androstenone and another odorant chemical, which is recognized by OR1I1, may play an important role in stallion testes.","PeriodicalId":33755,"journal":{"name":"Journal of Animal Reproduction and Biotechnology","volume":"68 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75701573","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Traf4 is required for tight junction complex during mouse blastocyst formation 在小鼠囊胚形成过程中，Traf4是紧密连接复合体所必需的

Journal of Animal Reproduction and Biotechnology Pub Date : 2021-12-31 DOI: 10.12750/jarb.36.4.307

Jian Lee, I. Choi

引用次数: 0

Effect of antibodies binding to Y chromosome-bearing sperm conjugated with magnetic nanoparticles on bull sperm characteristics 结合Y染色体精子的抗体与磁性纳米粒子结合对公牛精子特性的影响

Journal of Animal Reproduction and Biotechnology Pub Date : 2021-12-31 DOI: 10.12750/jarb.36.4.239

So-Yeon Jo, Yong Hwangbo, Sang-Hee Lee, H. Cheong, Dong-Ku Kim, Choon-keun Park

引用次数: 1

The studies on characteristics of return to estrus in postpartum dairy cattle 产后奶牛回发情特征的研究

Journal of Animal Reproduction and Biotechnology Pub Date : 2021-12-31 DOI: 10.12750/jarb.36.4.230

Doo Kim, Ji-Hwan Lee, G. Jang, Eun-Jeong Choi, Jin-Ju Kim, Jian Lee, J. Son

引用次数: 1

Hormone induced recipients for embryo transfer in mice 激素诱导小鼠胚胎移植受体

Journal of Animal Reproduction and Biotechnology Pub Date : 2021-12-31 DOI: 10.12750/jarb.36.4.247

Yeonmi Lee, Eunju Kang

{"title":"Hormone induced recipients for embryo transfer in mice","authors":"Yeonmi Lee, Eunju Kang","doi":"10.12750/jarb.36.4.247","DOIUrl":"https://doi.org/10.12750/jarb.36.4.247","url":null,"abstract":"Assisted reproductive technologies (ARTs) have applications in a variety of fields including human clinics and basic science research (Mochida, 2020). In animals such as mice, ART is important for the conservation of genetic resources and the generation of genetically engineered animals (Hasegawa et al., 2017). Many ARTs have been developed, such as cryopreservation of sperm and embryos, superovulation, in vitro fertilization (IVF), embryo culture, and embryo transfer (ET) (Ha et al., 2013; Lee et al, 2015; Hasegawa et al., 2017; Lee et al., 2018; Barsuren et al., 2019; Chio et al., 2019; Kim and Park, 2019; Jung et al., 2020). The success of several ARTs such as cryopreservation or IVF and the production of genetically engineered mice is dependent upon the ability of successful pregnancy after ET, therefore ET is an indispensable related to ART in mice (Lamas et al., 2020). Many factors could affect successful pregnancy after ET, such as age or weight of the recipients, pseudopregnancy of recipients, the type of anesthetic and analgesic, genetic backgrounds of the embryo and recipient strains, embryo stage and quality, the number of transferred embryos, the genetic modifications of embryos, and the surgical technique (Bradford, 1979; Ertzeid and Storeng, 2001; Mahabir et al., 2018; Lamas et al., 2020). For conventional ET procedures, the preparation of pseudopregnant recipients is inefficient. Females at the Hormone induced recipients for embryo transfer in mice Yeonmi Lee and Eunju Kang* Department of Biomedical Science, College of Life Science, CHA University, Seongnam 13488, Korea Center for Embryo and Stem Cell Research, CHA Advanced Research Institute, CHA University, Seongnam 13488, Korea Original Article J Anim Reprod Biotechnol 2021;36:247-252 pISSN: 2671-4639 • eISSN: 2671-4663 https://doi.org/10.12750/JARB.36.4.247 JARB Journal of Animal Reproduction and Biotechnology","PeriodicalId":33755,"journal":{"name":"Journal of Animal Reproduction and Biotechnology","volume":"22 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-12-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86494186","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Genetic diversity and relationship of Korean chicken breeds using 12 microsatellite markers 利用12个微卫星标记分析韩国鸡品种的遗传多样性和亲缘关系

Journal of Animal Reproduction and Biotechnology Pub Date : 2021-09-30 DOI: 10.12750/jarb.36.3.154

Yesong Kim, J. Yun, Seon Jeong Moon, J. Seong, H. Kong

{"title":"Genetic diversity and relationship of Korean chicken breeds using 12 microsatellite markers","authors":"Yesong Kim, J. Yun, Seon Jeong Moon, J. Seong, H. Kong","doi":"10.12750/jarb.36.3.154","DOIUrl":"https://doi.org/10.12750/jarb.36.3.154","url":null,"abstract":"A number of Korean Chicken breeds were registered in Domestic Animal Diversity Information System (DAD-IS, http://dad.fao.org/) of the Food and Agriculture Organization (FAO). Evaluation of genetic diversity and relationship of local breeds is an important factor towards the identification of unique and valuable genetic resources. Therefore, this study aimed to analysis the genetic diversity and relationship of 22 Korean Chicken breeds using 12 microsatellite (MS) markers. The mean number of alleles for each variety was 5.52, ranging from a 3.75 (Leghorn F; NF) to a 7.0 (Ross). The most diverse breed was the Hanhyup3 (HCC), which had the highest expected heterozygosity (H Exp ) (0.754) and polymorphic information content (PIC) (0.711). The NF was the least diverse population, having the lowest H Exp (0.467) and PIC (0.413). As a result of the principal coordinates analysis (PCoA) and factorial correspondence analysis (FCA) confirmed that Hy-line Brown (HL) and Lohmann Brown (LO) are very close to each other and that Leghorn and Rhode Island Red (RIR) are clearly distinguished from other groups. Thus, the reliability and power of identification using 12 types of MS markers were improved, and the genetic diversity and probability of individual discrimination were confirmed through statistical analysis. This study is expected to be used as basic data for the identification of Korean chicken breeds, and our results indicated that these multiplex PCR marker sets will have considerable applications in population genetic structure analysis.","PeriodicalId":33755,"journal":{"name":"Journal of Animal Reproduction and Biotechnology","volume":"39 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90267944","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

TRPV1 activation induces cell death of TM3 mouse Leydig cells TRPV1激活诱导TM3小鼠间质细胞死亡

Journal of Animal Reproduction and Biotechnology Pub Date : 2021-09-30 DOI: 10.12750/jarb.36.3.145

Eun-Jin Kim, L. Dang, Marie Merci Nyiramana, A. Siregar, M. Woo, Chang-Woon Kim, D. Kang

引用次数: 1

Investigation of single nucleotide polymorphism in TSH-β and CaSR associated with body weight in Korean native chickens (Gray Brown) 韩国土鸡TSH-β和CaSR与体重相关的单核苷酸多态性研究(灰褐色)

Journal of Animal Reproduction and Biotechnology Pub Date : 2021-09-30 DOI: 10.12750/jarb.36.3.129

D. Oh, J. Ha, J. Yi, Daehyun Kim, S. Oh, Songmi Kim, Kyudong Han, Yong-Soo Park

引用次数: 0

DNA recombinase Rad51 is regulated with UVinduced DNA damage and the DNA mismatch repair inhibitor CdCl2 in HC11 cells 在HC11细胞中，DNA重组酶Rad51受紫外线诱导的DNA损伤和DNA错配修复抑制剂CdCl2的调控

Journal of Animal Reproduction and Biotechnology Pub Date : 2021-09-30 DOI: 10.12750/jarb.36.3.121

Hyeong-ju You, Ga-Yeon Kim, Seung-Yeon Kim, M. Kang

{"title":"DNA recombinase Rad51 is regulated with UVinduced DNA damage and the DNA mismatch repair inhibitor CdCl2 in HC11 cells","authors":"Hyeong-ju You, Ga-Yeon Kim, Seung-Yeon Kim, M. Kang","doi":"10.12750/jarb.36.3.121","DOIUrl":"https://doi.org/10.12750/jarb.36.3.121","url":null,"abstract":"Both first authors contributed equally to this work. ABSTRACT Increasing the efficiency of HR (homologous recombination) is important for a successful knock-in. Rad51 is mainly involved in homologous recombination and is associated with strand invasion. The HR-related mismatch repair system maintains HR fidelity by heteroduplex rejection and repair. Therefore, the purpose of this study is to control Rad51, which plays a critical role in HR, through UV-induced DNA damage. It is also to confirm the effect on the expression of MMR related genes (Msh2, Msh3, Msh6, Mlh1, Pms2) and HR-related genes closely related to HR through treatment with the MMR inhibitor CdCl2. The mRNA expression of Rad51 gene was confirmed in both HC11 cells and mouse testes, but the mRNA expression of Dmc1 gene was confirmed only in mouse testes. The protein expression of Rad51 and Dmc1 gene increased in UV-irradiated HC11 cells. After 72 hours of treatment with 1 μm of CdCl2, the mRNA expression level of Msh3, Pms2, and Rad51 decreased, but the mRNA expression level of Msh6 and Mlh1 increased in HC11 cells. There was no significant difference in Msh2 mRNA expression between CdCl2 untreated-group and the 72 hours treated group. In conclusion, HR-related gene (Rad51) was increased by UV-induced DNA damage. Treatment of the MMR inhibitor CdCl2 in HC11 cells decreased the mRNA expression of Rad51.","PeriodicalId":33755,"journal":{"name":"Journal of Animal Reproduction and Biotechnology","volume":"404 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77700103","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0