Hormone induced recipients for embryo transfer in mice

Abstract

Assisted reproductive technologies (ARTs) have applications in a variety of fields including human clinics and basic science research (Mochida, 2020). In animals such as mice, ART is important for the conservation of genetic resources and the generation of genetically engineered animals (Hasegawa et al., 2017). Many ARTs have been developed, such as cryopreservation of sperm and embryos, superovulation, in vitro fertilization (IVF), embryo culture, and embryo transfer (ET) (Ha et al., 2013; Lee et al, 2015; Hasegawa et al., 2017; Lee et al., 2018; Barsuren et al., 2019; Chio et al., 2019; Kim and Park, 2019; Jung et al., 2020). The success of several ARTs such as cryopreservation or IVF and the production of genetically engineered mice is dependent upon the ability of successful pregnancy after ET, therefore ET is an indispensable related to ART in mice (Lamas et al., 2020). Many factors could affect successful pregnancy after ET, such as age or weight of the recipients, pseudopregnancy of recipients, the type of anesthetic and analgesic, genetic backgrounds of the embryo and recipient strains, embryo stage and quality, the number of transferred embryos, the genetic modifications of embryos, and the surgical technique (Bradford, 1979; Ertzeid and Storeng, 2001; Mahabir et al., 2018; Lamas et al., 2020). For conventional ET procedures, the preparation of pseudopregnant recipients is inefficient. Females at the Hormone induced recipients for embryo transfer in mice Yeonmi Lee and Eunju Kang* Department of Biomedical Science, College of Life Science, CHA University, Seongnam 13488, Korea Center for Embryo and Stem Cell Research, CHA Advanced Research Institute, CHA University, Seongnam 13488, Korea Original Article J Anim Reprod Biotechnol 2021;36:247-252 pISSN: 2671-4639 • eISSN: 2671-4663 https://doi.org/10.12750/JARB.36.4.247 JARB Journal of Animal Reproduction and Biotechnology