{"title":"Production of Bioethanol From Kappaphycus alvarezii Algae by Using Pichia kudriavzevii Yeast","authors":"N. Fadillah, W. L. Taufan, M. Aska","doi":"10.20956/IJAB.V2I1.4391","DOIUrl":"https://doi.org/10.20956/IJAB.V2I1.4391","url":null,"abstract":"Kappaphycus alvarezii is a red algae that can be used as alternative raw material for bioethanol production. This is because K. alvarezii contains a high carbohydrate that reaches 60%. This study aims to determine the effect of fermentation nutrition and fermentation duration of hydrolysis results of K. alvarezii algae on pH changes, sugar levels, cell biomass and ethanol content. There are two methods was used in this research, namely hydrolysis method and fermentation method. The hydrolysis method was used α-amylases enzyme which is 150 KNU/L with 0.5%, 1.0%, 1.5% and 2.0% concentrations. The next method, K. alvarezii algae was fermented by Pichia kudriavzevii and used Gandasil-D® as an anorganic suplementation and yeast extract which is 0 g/L, 1 g/L, 2 g/L and 3 g/L. The results showed that K. alvarezii can be fermented into bioethanol after enzymatic hydrolysis process. The optimal ethanol content was produced at 48 hours of incubation duration.","PeriodicalId":33480,"journal":{"name":"International Journal of Applied Biology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42944432","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The Effect of Method and Ripening Duration on the Content of β-Carotene of Fe’i Banana (Musa troglodytarum L.)","authors":"Fredy Leiwakabessy","doi":"10.20956/IJAB.V2I1.4020","DOIUrl":"https://doi.org/10.20956/IJAB.V2I1.4020","url":null,"abstract":"Research was conducted to determine the effect of the method and duration of ripening on the content of β-carotene of Fe’i banana, as well as determine the method and duration of ripening is effective in maintaining the nutritional value of Fe’i Banana. Through laboratory experimental with randomized complete design consisting of 19 groups of treatments and three repetitions, the data obtained were analyzed using statistical tests ANOVA and continued with Duncan test. Results of the analysis showed that method ofripening statistically significant difference (p <0,05) on the content of β-carotene in the banana sticks sky. Long ripening length statistically significant difference (p <0, 05) on the content of β-carotene in the Fe’i Banana. Grilled ripening method is more effective against β-carotene content in the Fe’i Banana and takes1 0 minutes of ripening length more effectively on the content of β-carotene on a Fe’i Banana.","PeriodicalId":33480,"journal":{"name":"International Journal of Applied Biology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48347059","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Development Michrosphere Bioadhesive Delivery System From Secondary Metabolit Actynomycetes M10a Rhizosphere Mangrove As Supportive Therapy of Colon Cancer Patients","authors":"F. Basri","doi":"10.20956/IJAB.V2I1.4615","DOIUrl":"https://doi.org/10.20956/IJAB.V2I1.4615","url":null,"abstract":"Colon cancer is a cancer that occurs in the large intestine when abnormal cells grow in the colon or rectum layer. Inflammation Symptoms in the colon are usually characterized by a polyp that has cancer risk. Approximately 96% of colon cancer cases are caused by adenocarcinomas that develop from the glandular tissue. Utilization of secondary metabolites from Actynomycetes Mangrove forests can be used as supportive therapy for colon cancer patients that have already through apoptotic testing so it can directly treat the target of illness. This study was prepared in the form of Michrosphere Bioadhesive using secondary metabolite Actynomycetes M10a rhizosphere mangrove. The purpose of this study was to obtain the development of Michosphere Bioadhesive preparations from secondary metabolites Actynomycetes M10a rhizosphere mangrove as supportive therapy for colon cancer patients. Method of the research is base on Laboratory experiment which started with Purification of Actynomycetes isolate, Secondary Metabolite Fermentation, Michrosphere Bioadhesive Formulation and evaluation of Michrosphere Bioadhesive preparation. The concentration of secondary metabolite Actynomycetes M10a rhizosphere mangrove that been used for each formula are 400 μl, 500 μl, and 600 μl, where the percentage of submerged in F1, F2, and F3 are 93,94%, 96,96%, and 94, respectively, 12%. Particular Particle Size Distribution is F1 (3.1μm), F2 (4.4 μm), F3 (3.9 μm). The rest angles obtained are F1 (0.002O), F2 (0.002O), F3 (0.0016O), respectively. Applied efficiency was obtained by F1 (214 nm), F2 (210 nm), F3 (211 nm). Dissolution results show the absorbance results at 266 nm wavelength. From the results obtained that meet the specifications seen in Formula 2. Keywords: Colon Cancer, Actynomycetes M10a rhizosphere mangrove, Michosphere Bioadhesive.","PeriodicalId":33480,"journal":{"name":"International Journal of Applied Biology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42888615","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Production Banana Glucose Syrup with the α-Amylase Supplementation","authors":"Ashifa Trisnaputri","doi":"10.20956/ijab.v2i1.4753","DOIUrl":"https://doi.org/10.20956/ijab.v2i1.4753","url":null,"abstract":"Indonesia is one of the largest producing country of banana in the world, but approximately only 1,5 million tons were consumed. As one of the fruit which contains the highest carbohydrate, the overripe banana with the high contains of sugar has a potential to be developed as the raw material of glucose syrup. According to the research, the banana with a lot of bruise or black mark on the skin has 2,6% strach, 33,6% reduction sugar, and 53,2% sucrose. In the making of banana glucose syrup, it will use α-amylase to hydrolize the strach. Based on the reduction sugar analysis there are nosignificantly different between the addition of 0,25 ml and 0,5 ml α-amylase. The results has qualified the minimum standard of glucose percentage (min. 30%) based on the reference SNI 01-2985-1992. The utilization of overripe bananas will greatly improve our glucose syrup production and ensure more of the banana waste can be saved in the future.","PeriodicalId":33480,"journal":{"name":"International Journal of Applied Biology","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49588788","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}