{"title":"EPR Study of Paramagnetic Centers in Human Blood","authors":"T. Kubiak, R. Krzyminiewski, B. Dobosz","doi":"10.2478/ctb-2013-0006","DOIUrl":"https://doi.org/10.2478/ctb-2013-0006","url":null,"abstract":"Abstract Electron paramagnetic resonance investigations of paramagnetic centers in whole human blood were carried out at 170 K using X-band EPR spectrometer. The study included a group of patients and healthy volunteers. The EPR signals from high spin Fe3+ ions in transferrin (g = 4.2) and Cu2+ ions in ceruloplasmin (g = 2.05) are characteristic of each frozen blood sample. An overview of all recorded spectra revealed in several cases additional lines derived from high spin Fe3+ ions in methemoglobin (g = 5.8 - 6), free radicals (g = 2.002 - 2.005) and various low spin ferriheme complexes (g = 2.21 - 2.91). The lines from cytochromes (g = 3.03 and 3.27) were observed only twice. The EPR measurements have not confirmed the correlation between the occurrence of a particular type of low-spin iron complex and a specific disease entity. Moreover, the presence of EPR lines from trivalent iron also did not differentiate patients from healthy volunteers.","PeriodicalId":333495,"journal":{"name":"Current Topics in Biophysics","volume":"23 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128131999","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Application of the Laser Interferometry in Studies of Biophysical Model Systems","authors":"S. Wąsik","doi":"10.2478/ctb-2013-0005","DOIUrl":"https://doi.org/10.2478/ctb-2013-0005","url":null,"abstract":"Abstract In this paper some selected applications of laser interferometry method in studies of biophysical model systems are presented. With the aid of a laser interferometric method, specific experiments were performed, which confirm that the gravitational field significantly modifies the amount of transported substance and affects the concentration profiles as well as the time evolution of the concentration field. The laser interferometry was also used to investigation of diffusion of antibiotics (ciprofloxacin or ampicillin) into the water phase from mixtures of neutral or negatively charged liposomes, and antibiotic-liposome interactions. Differences in the diffusion kinetics of ciprofloxacin and ampicillin from liposomal solutions to the water phase were observed. Moreover, the amount of ampicillin and ciprofloxacin released from the anionic liposomal phase was higher than that from the neutral one.","PeriodicalId":333495,"journal":{"name":"Current Topics in Biophysics","volume":"4 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128489551","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}