Journal of Pharmacogenomics and Pharmacoproteomics最新文献_第10页

To Test or Not To Test: Colon Cancer Pharmacogenetics and Predisposition Genetics 检测或不检测:结肠癌药物遗传学和易感性遗传学

Journal of Pharmacogenomics and Pharmacoproteomics Pub Date : 2011-03-19 DOI: 10.4172/2153-0645.1000E101

L. Manace

引用次数: 3

Penicillin Production from Transformed Protoplast of Penicillium chrysogenum by Fermentation 由青霉菌原生质体转化发酵生产青霉素

Journal of Pharmacogenomics and Pharmacoproteomics Pub Date : 2010-12-04 DOI: 10.4172/2153-0645.1000102

M. Sukumar, M. Sundar, M. Sivarajan

{"title":"Penicillin Production from Transformed Protoplast of Penicillium chrysogenum by Fermentation","authors":"M. Sukumar, M. Sundar, M. Sivarajan","doi":"10.4172/2153-0645.1000102","DOIUrl":"https://doi.org/10.4172/2153-0645.1000102","url":null,"abstract":"The Protoplast was prepared from the Penicillium chrysogenum and confirmed by microscopic observation and strains from the Penicillium chrysogenum were intrafused and protoplast was regenerated. The Penicillin was produced by fermentation method. The DNA was isolated from the Sclerotium rolfsii and it was characterized by electrophoresis and Protoplast of Penicillium chrysogenum was transformed with DNA of Sclerotium rolfsii. Penicillin was produced from transformed protoplast of Penicillium chrysogenum and at last HPLC was used to quantify the penicillin. Penicil - lin was isolated and purified and injected into experimental animals, where it was found not only to cure infections but also to possess incredibly low toxicity for the animals. This fact ushered into being the age of antibiotic chemotherapy, and an intense search for similar antimicrobial agents of low toxicity to animals that might prove useful in the treatment of infectious disease. Penicillium and Cephalosporium molds produce beta-lactam antibiotics such as penicillin and cephalosporin and their relatives. They also produce the base molecule for development of semisynthetic beta-lactam antibiotics, such as amoxacillin and ampicillin. Beta-lactams are used to treat about one-third of outpatients with bacte - rial infections.","PeriodicalId":333396,"journal":{"name":"Journal of Pharmacogenomics and Pharmacoproteomics","volume":"12 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2010-12-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115670888","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

A High-throughput Real-time PCR Approach to Pharmacogenomics Studies 药物基因组学研究的高通量实时PCR方法

Journal of Pharmacogenomics and Pharmacoproteomics Pub Date : 1900-01-01 DOI: 10.4172/2153-0645.1000133

T. Hartshorne, F. Le, Jordan Lang, H. Leong, K. Hayashibara, D. Dewolf, Elliot J Shelton

{"title":"A High-throughput Real-time PCR Approach to Pharmacogenomics Studies","authors":"T. Hartshorne, F. Le, Jordan Lang, H. Leong, K. Hayashibara, D. Dewolf, Elliot J Shelton","doi":"10.4172/2153-0645.1000133","DOIUrl":"https://doi.org/10.4172/2153-0645.1000133","url":null,"abstract":"Advances in personalized medicine have led to an increase in pharmacogenomics studies that involve testing individuals for drug metabolism enzyme and transporter gene polymorphisms implicated in drug response. As a consequence, there is a growing demand for affordable, easy to use technologies with fast sample-to-results workflows that can accommodate testing customizable sets of target gene variants and a changeable number of samples. Additionally, data analysis tools are needed to facilitate translation of an individual’s genetic information to their diploid content of gene-level star allele haplotypes, which can be correlated with drug metabolism enzyme phenotypes. Here we describe the development of a comprehensive pharmacogenomics experiments workflow solution to meet this need. High quality data was generated from purified buccal swab DNAs run with TaqMan® SNP genotyping and copy number assays in OpenArray® and 384-well plate formats, respectively, on the QuantStudio™ 12K Flex system. Data analysis was accomplished using TaqMan® Genotyper™ Software to examine SNP genotyping assay results and CopyCaller® Software to examine copy number assay results, followed by translation of this genetic data for individual samples to star allele genotypes using the recently developed AlleleTyper™ Software. The specific TaqMan® SNP Genotyping and Copy Number Assays to gene variants used can be tailored to suit the needs of a given pharmacogenomics study. This low cost, high throughput pharmacogenomics workflow can be completed in a single day, from sample preparation to data analysis.","PeriodicalId":333396,"journal":{"name":"Journal of Pharmacogenomics and Pharmacoproteomics","volume":"36 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114494183","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10

Linking Histone Methylation to Active DNA Demethylation 将组蛋白甲基化与活性DNA去甲基化联系起来

Journal of Pharmacogenomics and Pharmacoproteomics Pub Date : 1900-01-01 DOI: 10.4172/2153-0645.1000E133

T. Fandy

引用次数: 0

The Sequential Combination Paradigm in Epigenetic Therapy 表观遗传治疗的顺序组合范式

Journal of Pharmacogenomics and Pharmacoproteomics Pub Date : 1900-01-01 DOI: 10.4172/2153-0645.1000E124

T. Fandy

{"title":"The Sequential Combination Paradigm in Epigenetic Therapy","authors":"T. Fandy","doi":"10.4172/2153-0645.1000E124","DOIUrl":"https://doi.org/10.4172/2153-0645.1000E124","url":null,"abstract":"Copyright: © 2012 Fandy TE. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Epigenetic therapy is a novel therapeutic approach that targets DNA methylation, histone modifications or microRNAs (miRNAs). Drugs targeting DNA methylation (azacitidine and decitabine) and histone acetylation (vorinostat) are currently FDA-approved for the treatment of Myelo Dysplastic Syndromes (MDS) and cutaneous T-Cell Lymphoma (CTCL), respectively. Drugs targeting miRNAs and other histone modifications are currently in preclinical and clinical trials. A central hypothesis in epigenetic therapy is the combination of epigenetic modifiers in a specific sequential order to achieve optimal expression of epigenetically silenced tumor suppressor genes. This paradigm was established based on the observation that inhibitors of class I & II Histone Deacetylase (HDAC) enzymes cannot re-express genes silenced by promoter hypermethylation [1]. The use of a DNA Methyl Transferase (DNMT) inhibitor followed by an HDAC inhibitor is required for the re-expression of genes silenced by promoter hypermethylation. Consequently, the sequential or overlapping combination of DNA hypomethylating agents and HDAC inhibitors has been utilized in several clinical trials based on this hypothesis [2,3].","PeriodicalId":333396,"journal":{"name":"Journal of Pharmacogenomics and Pharmacoproteomics","volume":"15 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123868140","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

The Achilles Heel of Cervical Cancer: An Overlooked Target? 宫颈癌的致命弱点:一个被忽视的目标?

Journal of Pharmacogenomics and Pharmacoproteomics Pub Date : 1900-01-01 DOI: 10.4172/2153-0645.1000E128

Alfonso González

引用次数: 2

Highlights of European Regulatory Experience in Pharmacogenomics 欧洲药物基因组学监管经验的亮点

Journal of Pharmacogenomics and Pharmacoproteomics Pub Date : 1900-01-01 DOI: 10.4172/2153-0645.1000130

K. Prasad

引用次数: 0

Pharmacogenomics and Pharmacoproteomics Studies ofPhosphodiesterase-5 (PDE5) Inhibitors and Paclitaxel Albumin-StabilizedNanoparticles as Sandwiched Anti-Cancer Nano Drugs between TwoDNA/RNA Molecules of Human Cancer Cells 磷酸二酯酶-5 (PDE5)抑制剂和紫杉醇白蛋白稳定纳米颗粒作为夹在人癌细胞两种odna /RNA分子之间的抗癌纳米药物的药物基因组学和药物蛋白质组学研究

Journal of Pharmacogenomics and Pharmacoproteomics Pub Date : 1900-01-01 DOI: 10.4172/2153-0645.1000E153

A. Heidari

{"title":"Pharmacogenomics and Pharmacoproteomics Studies ofPhosphodiesterase-5 (PDE5) Inhibitors and Paclitaxel Albumin-StabilizedNanoparticles as Sandwiched Anti-Cancer Nano Drugs between TwoDNA/RNA Molecules of Human Cancer Cells","authors":"A. Heidari","doi":"10.4172/2153-0645.1000E153","DOIUrl":"https://doi.org/10.4172/2153-0645.1000E153","url":null,"abstract":"One of the most important goals in the medicine, pharmacology, pharmaceutical, physiological, clinical, biological, medical and medicinal sciences, biochemistry, pharmacogenomics and pharmacoproteomics of anti-cancer Nano drugs such as phosphodiesterase-5 (PDE5) inhibitors and paclitaxel albuminstabilized nanoparticles is the treatment. The present methods and techniques of Nano-structuring anti-cancer Nano drugs, especially phosphodiesterase-5 (PDE5) inhibitors and paclitaxel albuminstabilized nanoparticles, are expected to reach their limitations in the next decades. The smallest anti-cancer Nano drugs are of about five nanometers wide and hundreds of millions of them maybe integrated on a single molecule. Below this size the controlled doping becomes more and more difficult. The next important step in the treatment might be done by reducing the anti-cancer Nano drugs such as phosphodiesterase-5 (PDE5) inhibitors and paclitaxel albuminstabilized nanoparticles to the scale of DNA/RNA molecules of human cancer cells (Figures 1 and 2). This new filed of medicine, pharmacology, pharmaceutical, physiological, clinical, biological, medical and medicinal sciences, biochemistry, pharmacogenomics and pharmacoproteomics is called pharmachemotherapy. Recently, several researchers have measured electron transport in single or small groups of bioorganic molecules such as DNA/RNA of human cancer cells connected to metal such as Cadmium or Ruthenium (Figure 3) [1-29].","PeriodicalId":333396,"journal":{"name":"Journal of Pharmacogenomics and Pharmacoproteomics","volume":"250 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"1900-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122637080","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 113

Role of Micro-RNAs 221/222 on Statin Induced Nitric Oxide Release in Human Endothelial Cells 微rna 221/222在他汀类药物诱导的人内皮细胞一氧化氮释放中的作用

Journal of Pharmacogenomics and Pharmacoproteomics Pub Date : 1900-01-01 DOI: 10.4172/2153-0645.1000131

C. Fajardo

引用次数: 0

Endoscopic Discectomy with Amniotic Allograft Tissue Implantation for Treatment of Lumbar Disc Herniation 内窥镜椎间盘切除术联合羊膜异体移植治疗腰椎间盘突出症

Journal of Pharmacogenomics and Pharmacoproteomics Pub Date : 1900-01-01 DOI: 10.4172/2153-0645.1000174

R. Panganiban

引用次数: 0