Biotechnology Journal International最新文献

{"title":"Evaluation of Saccharomyces cerevisiae Improved Strains Potential in the Bioethanol Production from Bagasse","authors":"Adegbehingbe Kehinde Tope, A. Johnson, Omodara Tolani Rachael","doi":"10.9734/bji/2023/v27i5693","DOIUrl":"https://doi.org/10.9734/bji/2023/v27i5693","url":null,"abstract":"Plant biomass can be utilized to produce bioethanol, because they are abundantly available in nature. The cost of ethanol production from lignocellulosic materials is relatively high with low yield. But this can be solved by strain improvement processes. This study is aimed at evaluate bioethanol production potential of improved strains of Saccharomyces cerevisiae developed through random mutagenesis. Bagasse was hydrolysed with 1% NaOH and 1.0M H2SO4 respectively for five days. The hydrolysed bagasse was saccharified using Aspergillus niger isolated from soil samples. Saccharomyces cerevisiae isolated from locally produced wines; sorghum (burukutu) oil palm wine (emu) and raphia palm wine (oguro) with the highest ethanol production (5.0g/ml) were used, and then treated with physical mutagen (ultraviolet light) and chemical mutagens (Acridine dye, Bromo acetaldehyde, dithiothreitol, Ketoconazole and Nitrous acid) respectively to develop mutant with high ethanol producing efficiency under varied operational parameters. Three mutant strains of Saccharomyces cerevisiae namely;- SUV, SCD and SCK produced higher volumes of ethanol (7.5 g/ml, 9.8 g/ml, 11.2 g/ml respectively). SCD and SCK were able to grow at 25% ethanol concentration indicating that they had higher ethanol tolerance ability than the other strains. The optimum temperature and pH for ethanol production by all the strains were 350C and 6.0 respectively. The improved strains of Saccharomyces cerevisiae developed through random mutation techniques had produced more ethanol from the bagasse than the wild-type.","PeriodicalId":330877,"journal":{"name":"Biotechnology Journal International","volume":"114 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116446618","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Plant Growth Regulators on Callus Induction of Black Rice (Oryza sativa L.)","authors":"Rajkumari Sarita Devi, B. Roy, Gadge Sushan Sundarrao, U. Maity","doi":"10.9734/bji/2023/v27i5692","DOIUrl":"https://doi.org/10.9734/bji/2023/v27i5692","url":null,"abstract":"Aims: Black rice of Manipur is known for its aroma and nutritional quality. As per the reports on tissue culture of rice, callus induction response of traditional cultivars was much lower than the modern high yielding varieties. Considering the importance of the black rice, and effort was taken to standardized callus induction protocol of black rice of Manipur. \u0000Study Design:  Complete Randomized Design. \u0000Place and Duration of Study: Department of Seed Science and Technology, Faculty of Agriculture, Uttar Banga Krishi Viswavidyalaya, Pundibari, Cooch Behar 736165, West Bengal, India, between June 2020 and July 2023. \u0000Methodology: Mature seeds of two black rice varieties used in this work. The seeds were dehusked manually, washed with running tap water. Then sterilized with 1% bavistin and again washed with running tap water. Seeds were surface sterilized with 0.2% HgCl2 for 15 minutes. Sterilised seeds were rinsed with sterile distilled water inside laminar air flow hood. The seeds were blotting dry using autoclaved tissue paper and sterile seeds were cultured with the scutellum pointing up wards on callus induction medium fortified with different concentrations and combinations of plant growth regulators. \u0000Results: MS medium fortified with 2,4-D was found to be better than picloram and TDZ to induce callus and the 2,4-D @ 1.00 mg/L and 2,4-D 2.00 mg/L was found to be the best concentrations. Callus health was also good when MS medium invigorated with 2,4-D. \u0000Conclusion: An efficient embryogenic callus induction and plantlet regeneration protocol for black rice was established by using mature seeds. In callus induction studies, with different concentrations of PGR, 2,4-D, higher callus induction frequency was observed with 1.00 mg/L 2,4-D and 2,4-D @ 2 mg/L for both rice varieties.","PeriodicalId":330877,"journal":{"name":"Biotechnology Journal International","volume":"28 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"126963412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Effect of Jingdusha on Cucumber Chlorophyll Fluorescence Parameters under Corynespora cassiicola Stress","authors":"Jinghang Hong, Zhangtong Ma, J. Zhao, Junqiu Li, T. Song","doi":"10.9734/bji/2023/v27i5691","DOIUrl":"https://doi.org/10.9734/bji/2023/v27i5691","url":null,"abstract":"Aims: The paper aimed to clarify the effect of Jingdusha on chlorophyll fluorescence parameters of cucumber seedlings under Corynespora cassiicola stress. \u0000Study Design: We applied the method of artificial inoculation in the pot and analyzed the changes in the content of chlorophyll and chlorophyll fluorescence parameters. \u0000Place and Duration of Study: In 2018, these experiments were conducted in the College of Bioscience and Biotechnology of Shenyang Agricultural University (Lab 240). \u0000Methodology: The seedlings in the two-leaf period were induced by 2.0 mL·L-1 of Jingdusha, then inoculated C. cassiicola for 24 h. Cucumber seedlings of each treatment group were randomly selected for the content of chlorophyll and chlorophyll fluorescence parameters determination. The leaves of cucumber seedlings in each treatment group were randomly collected at 1 d, 3 d, 5 d, 7 d and 9 d after inoculation for the determination of the content of chlorophyll and chlorophyll fluorescence parameters. \u0000Results: When C. cassiicola infects cucumber, Jingdusha can effectively increase the energy conversion efficiency of the PSII central response and the potential activity of PSII, besides, the photosynthetic reaction activity did not change significantly in the early stage, but increased significantly in the later stage and rescure the degree of photosynthetic structural damage. \u0000Conclusion: In conclusion, under C. cassiicola stress, plant immune resistant inducer Jingdusha can effectively increase the capacity of PSII and the efficiency of PSII operation under the light in cucumber seedlings, reduce the damage of photosynthetic structure, increase the ability of PSII to accept and transfer electrons, as well as reduce the degradation of chlorophyll and the dissipation of light energy under C. cassiicola stress.","PeriodicalId":330877,"journal":{"name":"Biotechnology Journal International","volume":"27 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-07-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"117162344","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Growth, Yield and Nutritional Quality of Pleurotus pulmonarius and Pleurotus ostreatus, Grown on Different Substrates Amended with Wheat Bran","authors":"J. A. Obiaigwe, C. Adenipekun, Samson O. Egbewale, G. Aruwa","doi":"10.9734/bji/2023/v27i4690","DOIUrl":"https://doi.org/10.9734/bji/2023/v27i4690","url":null,"abstract":"Pleurotus species (oyster mushroom) cultivation is receiving global attention owing to their cheap labour requirements and nutritional/health benefits. In this study, 10 g of Pleurotus pulmonarius and Pleurotus ostreatus each were cultivated on 400 g of sugarcane bagasse and cotton waste individually. Each substrate was supplemented with wheat bran separately at varying compositions (0%, 5%, 10%, 15% and 20% w/w) in triplicate and incubated at 28 ± 2°C for 35 days. After incubation, the mushroom growth parameters and qualities (yields, minerals, and proximate composition) were evaluated. Overall, P. pulmonarius cultivated on sugarcane bagasse with wheat bran additives, irrespective of percentage concentration, had better mushroom quality/size, yields, biological efficiency, mineral content, and proximate composition than P. ostreatus. P. ostreatus cultivated on cotton waste performed best compared to P. pulmonarius at flush 1 (P ≤ 0.05). Also, relative low calcium, magnesium, and phosphorus contents were observed on both substrates but were significantly rich in potassium (1.32 – 6.817%) and protein (26.60 – 30.46%) contents. Thus, this study will guide farmers in selecting mushroom strains, substrates and percentage additives for a healthy and vigorous mushroom.","PeriodicalId":330877,"journal":{"name":"Biotechnology Journal International","volume":"221 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121860416","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Extended-Spectrum Beta-lactamase and Metallo-beta-lactamase Production among Escherichia coli and Klebsiella pneumoniae Strains from Urine of Pregnant Women in Afikpo, Ebonyi State, Nigeria","authors":"O. Uzochukwu, Okonkwo Evelyn Ijeoma, Ogundapo Solomon Segun, Nkama Onochie Jeff., Uchendu Damian Odochi, Eze Blessing Nwadimkpa","doi":"10.9734/bji/2023/v27i4689","DOIUrl":"https://doi.org/10.9734/bji/2023/v27i4689","url":null,"abstract":"Aim: The aim of this study was to determine the occurrence of Extended-spectrum beta-lactamase (ESBL) and Metallo-beta-lactamase (MBL) among Escherichia coli and Klebsiella pneumoniae strains from pregnant women attending Mater Misericordia Hospital Afikpo, Ebonyi state, Nigeria. \u0000Study Design: This is a laboratory based prospective study carried out on pregnant women suspected of having urinary tract infection and was requested to undergo diagnosis at microbiology laboratory of the hospital. \u0000Place and Duration of Study: The study was conducted in the Department of Science Laboratory Technology, Akanu Ibiam Federal Polytechnic, Unwana, Afikpo, Ebonyi State, Nigeria from October, 2022 to January, 2023. \u0000Methodology: Clean-catch midstream urine samples were collected from 206 pregnant women suspected of having urinary tract infection and were requested to undergo medical diagnosis at microbiology laboratory of the hospital. The urine samples were processed following standard microbiological procedure. Antimicrobial susceptibility testing was determined using the disc diffusion method, while ESBL phenotypes were determine by the Double-Disc Synergy Test (DDST). Disc potentiation test was performed to check for MBL production. \u0000Results: Out of the 206 urine samples processed, 24 (11.7 %) E. coli and 12 (5.8 %) K. pneumoniae were isolated. The antimicrobial susceptibility of the isolates recorded a 100 % resistance with Amoxicillin/Clavulanic acid and Cotrimoxazole.  The Gram-negative isolates showed a high sensitivity of 100 % to Netilmicin, Meropenem and Ofloxacin. Overall, 35 (97.2 %) multidrug resistance (MDR) was observed of the bacteria isolates. A total of 9 (37.5 %) E. coli and 4 (33.3 %) K. pneumoniae was found positive for ESBL production whereas, 5 (20.8 %) E. coli and 2 (16.7 %) K. pneumoniae were MBL positive. \u0000Conclusion: The level of drug resistance in this study underscores the need for regular surveillance for effective management of urinary tract infection in pregnancy.","PeriodicalId":330877,"journal":{"name":"Biotechnology Journal International","volume":"131 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-07-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116721559","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of Total Hydrocarbon Contents in the Leaves of Selected Crops Grown on Crude Oil Polluted Agricultural Soil","authors":"Mercy Ngozi Madubuike, T. E. Ogbulie, Chidinma Alvana Unegbu, Janet Damilola Johnson, Chimela Emmanuel Ibe","doi":"10.9734/bji/2023/v27i4688","DOIUrl":"https://doi.org/10.9734/bji/2023/v27i4688","url":null,"abstract":"Aim: The Total Hydrocarbon Content (THC) levels in the leaves of three edible plants (Allium cepa, Telfairia occidentalis and Zea mays) grown on soil polluted with 100 ml of crude oil were examined. \u0000Study Design: This experiment was conducted in two groups where the soil samples were polluted before planting (PB) and polluted 2 weeks after planting (PA). \u0000Place and Duration of Study: The research was carried out at the Federal University of Technology, Owerri (FUTO), Imo State, Nigeria, within a period of 4 weeks based on each group’s pollution time. \u0000Methodology: The tests for total hydrocarbon content (THC) were analyzed at different wavelengths for polluted soil and leaf samples using UV-Vis spectrophotometric method. Soil THC tests were determined on days 0, 14 and 28 respectively while THC tests on leaves of study plants were assessed on day 14 and 28. \u0000Results: The total hydrocarbon content values of the unpolluted soil (UPS) and the non-planted polluted soil (PS) subjected to this analysis on day zero were (UPS = 96.38 mg/kg; PS = 1082.80 mg/kg). However, results obtained on day 14 and 28 showed significant difference (P < 0.05) between the unpolluted soil sample (UPS) and all the planted polluted soil for Telfairia occidentalis (TOPB, TOPA), Allium cepa (ACPB, ACPA), Zea mays (ZMPB, ZMPA) as well as, the non-planted polluted soil (PS). For the leaf samples, TOPB had the highest THC value of 14.47mg/kg and 36.73 mg/kg for day 14 and 28 respectively while ZMPB had the lowest value of 5.38mg/kg at day 14 and ZMPA, the least THC value of 7.76mg/kg at day 28. \u0000Conclusion: Based on the varying THC levels observed in the leaves of crops used for this study; it was connoted that, bioaccumulation of hydrocarbons depends on the plants phytoremediation capability and the mode of pollution as observed in TOPB.","PeriodicalId":330877,"journal":{"name":"Biotechnology Journal International","volume":"753 ","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-07-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133970030","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of Spent Lubricating Oil Spiked Compost on Microbial Counts and Hydrocarbon Degradation Rate in Soils","authors":"M. Ntekpe, M. Ekpo, U. Ndubuisi-Nnaji, E. O. Mbong, E. Ntino","doi":"10.9734/bji/2023/v27i4687","DOIUrl":"https://doi.org/10.9734/bji/2023/v27i4687","url":null,"abstract":"This study evaluated the influence of organic fertilizers produced from spent lubricating oil (SLO) spiked aerobic composting technique on hydrocarbon degradation rate in soils. The compost windrows (Ft2 and Ft4), consisting of kitchen and agricultural wastes, were spiked with varying concentrations (2% and 4%) of SLO. The resultant organic fertilizers were employed as amendment in pollution simulated potted soils laid out in a complete randomized block design with three replications for 90 days. Results revealed higher counts of hydrocarbon utilizing microbes (HUB: 4.2±0.02×104cfu/g in Ft2, 3.0±0.02×104cfu/g in Ft4; HUF: 3.9±0.2×104cfu/g in Ft2, 2.5±0.02×104cfu/g in Ft4) in spiked compost compared to the control, Ft0 (HUB: 7.9±0.02×103cfu/g; HUF: 6.0±0.2×103cfu/g). Mean count in amended soils reflected a dose-dependent increase which followed the trend: Ft2 ˃ Ft0 ˃ Ft4 for the 5% (3.7×108 cfu/g), 10% (9.2×107 cfu/g) and 15% (6.9×107 cfu/g) levels of fertilizer treatments respectively. There was a significant (P<0.05) reduction in the TPH content of soils after 90days treatment with organic fertilizers. Generally, remediation efficiency followed the order: Ft2 ˃ Ft0 ˃ Ft4, with the highest (11.51%) achieved at 5% Ft2 application. Spiking technique was responsible for the higher counts of hydrocarbon utilizing microbes and enhanced bioremediation associated with the use of fertilizers Ft2.","PeriodicalId":330877,"journal":{"name":"Biotechnology Journal International","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-06-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114186401","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Cyanobacterial Combinations on Peanut Yield","authors":"G. M. Salem, A. A. Shaheen, M. F. Ghazal","doi":"10.9734/bji/2023/v27i4686","DOIUrl":"https://doi.org/10.9734/bji/2023/v27i4686","url":null,"abstract":"The characteristics of two cyanobacterial strains, Anabaena oryzae and Nostoc muscorum, were studied in order to use them as biofertilizers in a field experiment conducted in the two winter seasons of 2021 and 2022 at the Ismailia Agricultural Research Center Station to study the effect of both strains on peanut plant in sandy soil. Cyanobacterial strains were used individually by coating seed, soil drench, and foliar applications, as well as mixed applications of two strains in various ways. Both cyanobacterial strains morphological examination revealed that they both have heterocysts, nitrogen, phosphorus, and potassium in their culture filtrate, and they were able to produce chlorophyll a and phosphatase enzymes. The results of an agricultural experiment showed that using Nostoc muscorum and Anabaena oryzae separately had a positive effect on peanut plants in a variety of applications, but combining both of these applications with 75% nitrogen increased the growth traits, nutrient contents, and soil biological activities in both peanut plants and their rhizosphere soil. The soil drench treatment with A. oryzae and Nostoc muscorum plus 75% nitrogen produced the highest growth results and peanut yields in a single application. The A. oryzae Soil Drench Application (S) + N. muscorum Foliar Application (F) with 75% N reported the best outcomes in mixed treatments. However, compared to single applications, all blended applications displayed better growth and yield characteristics. The results of the study suggest that employing cyanobacteria in a mixed application will enhance its advantages over a single use.","PeriodicalId":330877,"journal":{"name":"Biotechnology Journal International","volume":"55 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134458555","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synergetic Influence of Some Tropical Leaf Meals and Garlic on the Haemato-biochemical Parameters and Antioxidant Activities of Weaner Pigs","authors":"M. Adegbenro, A. Ayeni, M. O. Olasunkanmi, O. Olabanji, F. E. Ajayi, T. Oladayo, O. Olaleye, J. Agbede","doi":"10.9734/bji/2023/v27i3684","DOIUrl":"https://doi.org/10.9734/bji/2023/v27i3684","url":null,"abstract":"Aim: This experiment was designed to study the effects of using garlic-composite leaf meals produced from four (4) different leaves and garlic: bitter leaf (Vernonia amygdalina), scent leaf (Ocimum gatissimum), Neem leaf (Azadirachta indica), Moringa leaf (Moringa oleifera) and Garlic (Allium sativum) as a premix in the diets of growing pigs. The leaves and garlic were air dried, milled and sieved separately. Thereafter the leaves and garlic were mixed in the ratio of 4 (Vernonia amygdalina): 3 (Moringa oleifera): 1 (Ocimum gatissimum): 1 (Azadirachta indica) and 1 (Allium sativum) to produce the garlic-composite tropical leaf meals. Individual leaves and their composite mix were analyzed for proximate, mineral, antioxidant and the phytochemical components of the leaves were determined using GCMS and other standard methods. \u0000Methodology: Eighteen large white weaner-pigs of eight weeks were allocated in a completely randomized design for this experiment comprising three treatments and three replicates with two pigs per replicate. The average weight of the pigs were 13 kg. Basal diet were formulated and subdivided into three portions in which garlic-composite leaf meals were fed at 0g/kg, 10g/kg, and 20g/kg were used as an additives to the diets of weaner pigs and the diets were designated as I, II and III respectively. The pigs were then assigned to these 3 dietary treatments which were fed to the pigs at 5% of their body weight for 12 weeks experimental period. Water was supplied ad libitum throughout the experimental period. All data were subjected to analysis of variance. \u0000Results: Dietary inclusion of GCLM on haematology, serum biochemistry indices  and antioxidants significantly (P<0.05) affected the Packed Cell Volume (%), Mean Corpuscular Volume (fl) Lymphocytes (%), Granulocytes (%), Alanine aminotransferase (IU/L), Aspartate aminotransferase (IU/L), Total Protein (g/l)  and catalase (Ku) of the experimental pigs. \u0000Conclusions: It could be concluded within the limit of this study, that garlic-composite leaf meals had high nutrient potentials for pigs and could completely help growing pigs to improve in body weight as the composite leaf meals increases in pig diets.","PeriodicalId":330877,"journal":{"name":"Biotechnology Journal International","volume":"49 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114590256","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Control Efficacy of Jingdusha against Corynespora cassiicola (Hyphomycetales: Dematiaceae) on Cucumber","authors":"Zhangtong Ma, Jinghang Hong, J. Zhao, Mingke Zuo, T. Song","doi":"10.9734/bji/2023/v27i3683","DOIUrl":"https://doi.org/10.9734/bji/2023/v27i3683","url":null,"abstract":"Aims: The paper aimed to clarify the effect of cucumber target leaf spot (TLS) under the Jingdusha (JDS) treatment. \u0000Study Design: We applied the method of artificial inoculation in the pot, and analyzed the changes in growth indexes and physiological characteristics. \u0000Place and Duration of Study: In 2018, these experiments were conducted in College of Bioscience and Biotechnology of Shenyang Agricultural University (Lab 240). \u0000Methodology: The seedlings in the two-leaf period were induced by the best application scheme of JDS, then inoculated Corynespora cassiicola for 24 h. Cucumber seedlings of each treatment group were randomly selected for photographing and growth index determination after inoculation for 5 d. The leaves of cucumber seedlings in each treatment group were randomly collected at 1 d, 3 d, 5 d, 7 d, and 9 d after inoculation for the determination of physiological and biochemical indicators. \u0000Results: When C. cassiicola infects cucumber, JDS can effectively improve the growth and photosynthetic pigment content of cucumber, reduce the degradation of chlorophyll (Chl) under the stress of C. cassiicola, strengthen the variety of metabolic responses in the plant, repair the enzyme protection system of cucumber leaves, reduce the accumulation of reactive oxygen species, shorten the process of membrane lipid peroxidation in blades. \u0000Conclusion: Taken together, these results suggest that JDS can improve the resistance of cucumber seedlings to C. cassiicola by regulating growth indexes and physiological characteristics. This work will provide a theoretical basis for further elucidating the molecular mechanism of JDS in cucumber defense against C. cassiicola.","PeriodicalId":330877,"journal":{"name":"Biotechnology Journal International","volume":"6 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124801501","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}