Veterinaria Montevideo最新文献_第2页

Estudio retrospectivo de hallazgos ultrasonográficos en útero y ovarios de perras no preñadas para determinar la frecuencia de aparición de las principales patologías 对未怀孕母狗子宫和卵巢超声检查结果的回顾性研究，以确定主要疾病的发病频率

Veterinaria Montevideo Pub Date : 2022-06-01 DOI: 10.29155/vet.58.217.5

引用次数: 0

Situación sanitaria de las abejas melíferas en Uruguay Novedades de la última década Autores/as 乌拉圭蜜蜂的健康状况过去十年的发展作者/as

Veterinaria Montevideo Pub Date : 2022-06-01 DOI: 10.29155/vet.58.217.4

引用次数: 5

Clostridioides (Clostridium) difficile en porcinos Caracterización, consideraciones epidemiológicas y resistencia a los antimicrobianos 猪艰难梭菌的特征、流行病学考虑和抗菌素耐药性

Veterinaria Montevideo Pub Date : 2022-03-30 DOI: 10.29155/vet.58.217.2

引用次数: 0

Síndrome de Insuficiencia Adrenocortical en un caballo de resistencia 耐力马的肾上腺皮质功能不全综合征

Veterinaria Montevideo Pub Date : 2022-03-03 DOI: 10.29155/vet.58.217.1

引用次数: 0

Presentaciones en congresos: ¿cuál es su objetivo, y cómo deben considerarse en una evaluación? 会议报告:它们的目标是什么?在评估中应如何考虑?

Veterinaria Montevideo Pub Date : 2021-10-01 DOI: 10.29155/vet.57.216.6

引用次数: 0

Utilidad del proteinograma electroforético capilar en el diagnóstico de la Peritonitis Infecciosa Felina (PIF) no efusiva: estudio de caso 毛细管电泳蛋白图在猫感染性非渗出性腹膜炎诊断中的应用:个案研究

Veterinaria Montevideo Pub Date : 2021-10-01 DOI: 10.29155/vet.57.216.5

引用次数: 0

Outer membrane protein CD of Moraxella bovis as a potential immunogen against infectious bovine keratoconjunctivitis 牛莫拉菌外膜蛋白CD作为抗传染性牛角膜结膜炎的潜在免疫原

Veterinaria Montevideo Pub Date : 2021-10-01 DOI: 10.29155/vet.57.216.4

Sofía Acquistapace, A. Umpiérrez, Sofía Fernández-Ciganda, J. Sotelo-Silveira, P. Zunino

{"title":"Outer membrane protein CD of Moraxella bovis as a potential immunogen against infectious bovine keratoconjunctivitis","authors":"Sofía Acquistapace, A. Umpiérrez, Sofía Fernández-Ciganda, J. Sotelo-Silveira, P. Zunino","doi":"10.29155/vet.57.216.4","DOIUrl":"https://doi.org/10.29155/vet.57.216.4","url":null,"abstract":"Infectious bovine keratoconjunctivitis (IBK) is a severe ocular disease that affects cattle and has a significant economic impact worldwide. The principal etiological agent of IBK is Moraxella bovis. Another species, Moraxella bovoculi, has also been reported as a possible etiological agent. Due to the health problems caused by IBK, it is necessary to develop effective vaccines to prevent the disease. Outer membrane protein-CD (OmpCD) has exhibited potential as a protective antigen in related species such as Moraxella catarrhalis. The objective of this study was to produce a recombinant M. bovis OmpCD and evaluate its immunogenicity in CD-1 mice. The ompCD gene was amplified in all of the M. bovis and M. bovoculi strains studied, and sequence alignment revealed highly conserved regions. The reactivity of M. bovis recombinant OmpCD was seen when sera and tears of diseased bovines were used in Western blots. CD-1 mice were immunized and the antibody response was analyzed in serum and bronchoalveolar lavage (BAL) fluid. Cytokine levels in the BAL fluid were also determined. The trials in mice allowed us to demonstrate that OmpCD administered systemically induced a significant increase in sera IgG specific antibodies while the intranasal immunization induced a significant increase in IgA and IL-4 levels in BAL. Moreover, the combination of OmpCD and a mucosal adjuvant significantly increased the IgA response in BAL. These results encourage further studies to assess the role of OmpCD as a possible vaccine candidate against IBK, using challenge models in cattle.","PeriodicalId":32689,"journal":{"name":"Veterinaria Montevideo","volume":"7 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84041613","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Diagnóstico molecular de Neospora caninum en fetos abortados espontáneamente en bovinos de Uruguay.

Veterinaria Montevideo Pub Date : 2021-09-15 DOI: 10.29155/vet.57.216.3

C. Rodríguez, Javier Regidor-Cerrillo, Cristina Easton, Florencia Pieruccioni, Agustín Romero, P. Peraza, América Mederos, F. Dutra

{"title":"Diagnóstico molecular de Neospora caninum en fetos abortados espontáneamente en bovinos de Uruguay.","authors":"C. Rodríguez, Javier Regidor-Cerrillo, Cristina Easton, Florencia Pieruccioni, Agustín Romero, P. Peraza, América Mederos, F. Dutra","doi":"10.29155/vet.57.216.3","DOIUrl":"https://doi.org/10.29155/vet.57.216.3","url":null,"abstract":"espanolEn Uruguay el diagnostico de rutina de Neospora caninum se realiza fundamentalmente por estudios histopatologicos de los fetos abortados complementando esta informacion con resultados deserologia y/o Inmunohistoquimica (IHQ). El objetivo de este estudio fue estandarizar un protocolo de amplificacion de secuencias mediante PCR (polymerase chain reaction) para la deteccion de Neospora caninum en organos de fetos abortados espontaneamente en bovinos para su incorporacion a la rutina diagnostica. Para dicho proposito se realizo un estudio retrospectivo utilizando muestras congeladas de archivo de un periodo comprendido entre el ano 2013 y 2016, con diagnostico positivo o negativo a Neospora caninum. Los resultados mostraron que de 31 muestras positivas a Neospora caninum por las tecnicas de rutina, 26 fueron positivas por PCR (84%), demostrando una alta sensibilidad de diagnostico de la tecnica. Todas las muestras con diagnostico negativo mediante histopatologia (n=20) fueron confirmadas como negativo mediante PCR. La comparacion de los resultados de PCR con los de histopatologia, mediante el test de Cohen’s Kappa, indico un acuerdo muy alto (81%, p EnglishThe diagnosis of Neospora caninum in Uruguay is carried out by histopathological studies of aborted fetuses, complementing this information with serology test and/or immunohistochemistry (IHC) results. The aim of this study was to standardize a proto-col of polymerase chain reaction (PCR) to detect of N. caninum in organs of spontaneously aborted bovine fetus. A retrospective study was conducted using frozen samples from a period between 2013 and 2016, with positive or negative diagnosis of N. cani-num. Results showed that 26 of 31 samples positives for N. cani-num by routine techniques were positive by PCR, demonstrating a high sensitivity of the PCR. All negative samples by Histopa-thology (n=20) were confirmed as negative by PCR. Cohen’s Kappa test indicated a very high agreement (81%, p","PeriodicalId":32689,"journal":{"name":"Veterinaria Montevideo","volume":"125 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80542306","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Comparación de ELISA indirecto en suero sanguíneo versus ELISA indirecto en leche, para el diagnóstico de paratuberculosis en bovinos de tambo.Realizada en la provincia de Buenos Aires, Argentina 血清间接ELISA法与牛奶间接ELISA法诊断坦博牛副结核的比较。发生在阿根廷的布宜诺斯艾利斯省

Veterinaria Montevideo Pub Date : 2021-08-26 DOI: 10.29155/vet.57.216.2

Pedro Sebastián Sosa, M. F. A. Pinedo, L. D. Paolo, Luis María Peralta, Gabriel Eduardo Travería

{"title":"Comparación de ELISA indirecto en suero sanguíneo versus ELISA indirecto en leche, para el diagnóstico de paratuberculosis en bovinos de tambo.Realizada en la provincia de Buenos Aires, Argentina","authors":"Pedro Sebastián Sosa, M. F. A. Pinedo, L. D. Paolo, Luis María Peralta, Gabriel Eduardo Travería","doi":"10.29155/vet.57.216.2","DOIUrl":"https://doi.org/10.29155/vet.57.216.2","url":null,"abstract":"espanolEl objetivo de este trabajo fue comparar las caracteristicas ope-rativas de un ELISA en suero, con un ELISA en leche, para el diagnostico de paratuberculosis bovina. Utilizando analisis ROC, el area bajo la curva fue de 0,84 para ELISA en suero y 0,88 para ELISA en leche, sin diferencias significativas (p= 0,30) entre ambas AUC; la sensibilidad fue de 91,7% y 87,5%, la especificidad de 82,0% y 86,5% para ELISA en suero y ELI-SA en leche respectivamente. Tambien se consideraron como variables, los dias en lactancia y el numero de lactancias. Los dias en lactancia se analizaron con correlacion y regresion line-al simple, evidenciando una relacion positiva (p 2da lactancias. Los resultados preliminares obtenidos, demostraron que el ELISA en suero presenta caracteristicas ope-rativas similares al ELISA en leche, siendo el ELISA en leche una alternativa practica y menos invasiva al muestreo EnglishThe aim of this paper was to compare the operational charac-teristics of serum ELISA with milk ELISA, for the diagnosis of bovine paratuberculosis. Using ROC analysis, the area under the curves were 0.84 in serum ELISA and 0.88 in milk ELISA, without significant differences (p= 0.30) between both AUC; the sensitivities were 91.7% and 87.5%, the specificities 82.0% and 86.5% for serum ELISA and milk ELISA respectively. In addi-tion, days in lactation and lactation numbers were also included as variables. Days in lactation were analyzed with correlation and simple linear regression, showing a positive relationship (p 2nd lactations. The preliminary results demon-strate that serum ELISA presents similar operational charac-teristics to milk ELISA, being milk ELISA a practical and less invasive alternative to sampling","PeriodicalId":32689,"journal":{"name":"Veterinaria Montevideo","volume":"49 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-08-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74618232","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Detección en la conjuntiva y el oído externo de especies potencialmente patógenas del género Mycoplasma en ovinos 绵羊结膜和外耳潜在致病性支原体物种的检测

Veterinaria Montevideo Pub Date : 2021-08-06 DOI: 10.29155/VET.57.216.1

N. Gaeta, M. Alemán, Jorge Timenetsky, L. Gregory

{"title":"Detección en la conjuntiva y el oído externo de especies potencialmente patógenas del género Mycoplasma en ovinos","authors":"N. Gaeta, M. Alemán, Jorge Timenetsky, L. Gregory","doi":"10.29155/VET.57.216.1","DOIUrl":"https://doi.org/10.29155/VET.57.216.1","url":null,"abstract":"Mycoplasmas are responsible for several infections in small ruminants. Potentially pathogenic mycoplasmas have already been detected in the external ear canal of Brazilian healthy goats and cattle, and in association with mites, but not in sheep. The present research aimed to evaluate the role of the conjunctiva and external ear as a silent source of micoplasmas in sheep. Ocular and external ear canal swab samples were obtained from forty-five male and female sheep. Molecular detection (PCR) and isolation of Mycoplasma spp. were performed. Minimum inhibitory concentration was performed using oxytetracycline, tylosin, chloramphenicol and nalidixic acid. We describe the isolation of Mycoplasma conjunctivae from the ocular conjunctivae of a healthy sheep, besides the molecular detection of M. agalactiae and M. mycoides subsp. capri in both anatomical sites in sheep from the State of Sao Paulo. Phylogenetic analysis revealed proximity and similarity with an American sheep and goat isolates. The ocular conjunctiva and the external ear of sheep are keepers of potentially pathogenic veterinary mycoplasmas which can infect vulnerable sheep.","PeriodicalId":32689,"journal":{"name":"Veterinaria Montevideo","volume":"12 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-08-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87561735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1